• Title/Summary/Keyword: leaf analysis

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Degree of Heterosis and Heterobeltiosis in F1 Hybrids of Eggplant (가지 F1세대의 잡종강세 정도)

  • Oh Ju-Sung;Hwang Pil-Seong;Chung Won-Bok
    • Journal of Life Science
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    • v.15 no.1 s.68
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    • pp.132-135
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    • 2005
  • This experiment was performed to obtain the basic information on heterosis and heterobeltiosis of quantitative characters in the eggplant by crossing of varieties. The heterosis and heterobeltiosis were estimated in Fl generation. The results were as follows, based on the graphical analysis, the characters, plant height, stem diameter, fruit weight, leaf length, leaf width, and fruit number per plant(yield) were assumed to inherit dominance, and fruit length and fruit diameter were found to inherit incomplete dominance. The degree of positive heterosis of plant height, stem diameter, fruit weight, fruit length, fruit diameter, leaf length, leaf width, and fruit diameter, leaf width, leaf length, fruit weight, and stem diameter showed negative heterobeltiosis.

STUDIES ON THE GENERIC ANALYSIS AMONG BURLEY, FLUE-CURED AND SUN-CURED TYPE TOBACCO II. Heterosis, Correlation and Combining Ability of $F_2$ Hybrid. (버어리종, 황색종, 양건종, 담배의 유전분석에 관한 연구 II. $F_2$의 잡종강세, 상관 및 조합능력)

  • 한철수
    • Journal of the Korean Society of Tobacco Science
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    • v.7 no.2
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    • pp.111-121
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    • 1985
  • The present study was carried out to obtain some basic informations of the breeding of tobacco varieties. Genetically divergent 8 varieties, 3 flue-cured, 2 burley and 3 sun-cured tobaccos, were used in half diallel cross. In order to analyze the heterosis, combining abilities, modes of inheritance and correlations for some agronomic and chemical characters, 8 parents and 28 $F_2$ were tested. The results obtained were summarized as follows: The percentages of heterosis for yield, stem diameter, internode length and total sugar content in $F_2$ hybrid were positive , where-as those for the other characters were negative. Yield had significant positive genotypic and phenotypic correlations with plant height, days to flower, leaves per plant, leaf length, leaf width and leaf shape index (Leaf length/leaf width). General (GCA) and specific combining abilities (SCA) for all characters of $F_2$ hybrid were significant. In the effect of GCA, yield was greater in combinations of Bulgaria and Xanthi, days to flower and leaves per plant were those of Burley 21 and Va 528, leaf length and width were those of Coker 139, respectively. In the effect of SCA, the major part of characters were greater than those of others in the combinations of Burley 21, Va 528, Hicks.

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Development of Granular Tea by Using Astringent Persimmon and Persimmon Leaves (떫은 감과 감잎을 이용한 과립차 개발에 관한 연구)

  • Byun, Gwang-In;Kwon, Yong-Ju;Park, Mi-Lan
    • Culinary science and hospitality research
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    • v.14 no.2
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    • pp.273-285
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    • 2008
  • The purpose of this study was to find appropriate processing condition of astringent persimmon and persimmon leaf granular tea. Under the condition of 4 hour extraction and $50^{\circ}brix$, extraction yield and concentrating of astringent persimmon were the greatest. Under the condition of 2 hour extraction and $7^{\circ}brix$, extraction yield and concentrating of persimmon leaf were the greatest. The optimum condition of granular tea with astringent persimmon and persimmon leaf added was the best in astringent persimmon concentrate of 16% and persimmon leaf concentrate of 4%. It had the best taste after the condition in a brix value of 16.15% and a pH of 5.57. The L-value of chromaticity was -79.13, a-value 2.27, and b-value 2.41. In the sensory properties correlation analysis of astringent persimmon and persimmon leaf granular tea, sweet taste, color acceptability, odor acceptability and taste accept-ability were positively significant correlation with most sensory properties. However, astringent taste was no significant correlation with sensory acceptability. Bitter taste and after taste were negatively significant correlation with most sensory properties.

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Composition of Leaf Surface Lipid in Flue-cured Tobacco and Their Changes during Flue-curing (황색종 잎담배의 엽면지질 성분 조성 및 건조중 변화)

  • 지상운;김도연;이문용;김영희;신승구
    • Journal of the Korean Society of Tobacco Science
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    • v.19 no.1
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    • pp.57-63
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    • 1997
  • The composition of leaf surface lipid in Flue-cured tobacco and their changes during curing was investigated. The flue-cured variety, Nicotiana tabacum cv. NC 82 was cultivated at Eumsung experiment station in 1996. The samples of riced leaves with different stalk Position(Lugs, Cutter, Leaf and Tips) and different curing stage at half yellowing(24hr), yellowing(48 hr) , color axing(72 hr), midrib drying(96 hr) and cured(120 hr) were collected for analysis of leaf surface lipid. $\alpha$ - and $\beta$ - 4, 8, 13-Duvatriene-1, 3-diol($\alpha$, $\beta$-DVT) were major components in leaf surface lipid extracted with methylene chloride and sugar ester was detected slightly DVT content was increased with ascending stalk position, but increasing trend of total hydrocarbon was not observed. While DVT was decreased throughout curing of tobacco leaves, hydrocarbon content did not show significant change during curing process. Twenty-two duvane compounds were detected by capillary GC in duvane fraction isolated from leaf surface lipid and of which 11 compounds were identified by GC-MS. These compounds were decreased with curing in all stalk position.

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Optimized Analytical Method of Ether Extract in Leaf Tobacco by XT15(ANCOM) (AOCS Method) (XT15(ANCOM)를 활용한 잎담배 에테르 추출물의 최적 분석방법 구명(AOCS Method))

  • Na, Seung-Ju;Lee, Jung-Lae;Ha, Ah-Rang;Kim, Suk-Jae;Seo, Jung-Kyu
    • Journal of the Korean Society of Tobacco Science
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    • v.32 no.2
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    • pp.88-94
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    • 2010
  • The ether extract(EE) of leaf tobacco contains diverse compounds such as lipid, resinoid, paraffin hydrocarbons and pigments. Although the correlation of EE concentertion with leaf tobacco has not been established clearly, it is known that in some concentration range, EE concentration of leaf tobacco exerts good influence on the quality of tobacco. Recently, The American Oil Chemist's Society introduced new method(Am 5-04; AOCS, 2009) to determine EE concentration by AT15 extractor. This method is based on extraction with organic solvent at relatively high temperature and pressure, which significantly reduces the extraction time and ensures safety. The aim of this study is to optimize analytical condition of AOCS method for analyzing EE concentration of leaf tobacco. When sample pre-drying time and extraction time of XT15 were set to 3 hour and 30 minute and 30 minute respectively, EE concentration obtained from AOAC method. Statistical analysis(T-test) showed that there is no difference(P>0.05) between EE concentrations from two methods.

Neopestalotiopsis Leaf Blight, an Emerging Concern on Leatherleaf Fern in Indonesia

  • Ani Widiastuti;Indah Khofifah Aruan;Alvina Clara Giovanni;Barokati Tsaniyah;Tri Joko;Achmadi Priyatmojo
    • Research in Plant Disease
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    • v.30 no.1
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    • pp.82-87
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    • 2024
  • Leatherleaf fern (Rumohra adiantiformis) is an important ornamental plant in Indonesia and global. Green fern leaves with bold dark green color with long shelf-life, attract florists as decoration. Indonesia is one important leatherleaf fern exporters, however currently an outbreak of leaf blight decreased production significantly. Initial symptom was reddish brown spots from edge of leaf, which was gradually followed by dark-brown necrotic lesions causing leaf blight and dried. This is a study to do Koch-Postulate approach and molecular identification, to identify the pathogen of the "new emerging disease" reported. Based on multigene analysis using primers from ITS, β-tub and tef1-α gene markers, the pathogen was identified as Neopestalotiopsis sp. All sequences have been deposited in GenBank with accession number of OR905551 (ITS), OR899817 (ßtubulin) and OR899816 (TEF). This Neopestalotiopsis leaf blight causes an emerging concern in leatherleaf fern in Indonesia and global biosecurity because it infected an export commodity.

Comparative Analysis of the Conserved Functions of Arabidopsis DRL1 and Yeast KTI12

  • Jun, Sang Eun;Cho, Kiu-Hyung;Hwang, Ji-Young;Abdel-Fattah, Wael;Hammermeister, Alexander;Schaffrath, Raffael;Bowman, John L.;Kim, Gyung-Tae
    • Molecules and Cells
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    • v.38 no.3
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    • pp.243-250
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    • 2015
  • Patterning of the polar axis during the early leaf developmental stage is established by cell-to-cell communication between the shoot apical meristem (SAM) and the leaf primordia. In a previous study, we showed that the DRL1 gene, which encodes a homolog of the Elongator-associated protein KTI12 of yeast, acts as a positive regulator of adaxial leaf patterning and shoot meristem activity. To determine the evolutionally conserved functions of DRL1, we performed a comparison of the deduced amino acid sequence of DRL1 and its yeast homolog, KTI12, and found that while overall homology was low, well-conserved domains were presented. DRL1 contained two conserved plant-specific domains. Expression of the DRL1 gene in a yeast KTI12-deficient yeast mutant suppressed the growth retardation phenotype, but did not rescue the caffeine sensitivity, indicating that the role of Arabidopsis Elongator-associated protein is partially conserved with yeast KTI12, but may have changed between yeast and plants in response to caffeine during the course of evolution. In addition, elevated expression of DRL1 gene triggered zymocin sensitivity, while overexpression of KTI12 maintained zymocin resistance, indicating that the function of Arabidopsis DRL1 may not overlap with yeast KTI12 with regards to toxin sensitivity. In this study, expression analysis showed that class-I KNOX genes were downregulated in the shoot apex, and that YAB and KAN were upregulated in leaves of the Arabidopsis drl1- 101 mutant. Our results provide insight into the communication network between the SAM and leaf primordia required for the establishment of leaf polarity by mediating histone acetylation or through other mechanisms.

Proteome analysis of sorghum (Sorghum bicolor L.) leaf in response to waterlogging stress

  • Yun, Min-Heon;Park, Hyeong-Jun;Jeong, Hae-Ryong;Roy, Swapan Kumar;Kwon, Soo Jeong;Chun, Hyen Chung;Cho, Seong-Woo;Woo, Sun-Hee
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.119-119
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    • 2017
  • Growth related to morphological and proteome response under waterlogging stress in sorghum has not yet been elucidated. Understanding how plants respond to waterlogging, the present study was conducted in seedlings leaf of the Nam-pung chal cultivar. Regarding 3-leaf stage of sorghum, stem length and plant height were slightly decreased in the treatments during ten days of waterlogging, and chlorophyll contents were also significantly different from 7 days of waterlogging treatment. The results observed from the present study were considered to be influenced by the waterlogging stress more in the $5^{th}$ leaf stage of the growth period of the sorghum, and as the waterlogging treatment progressed, the waterlogging stress gradually influenced the growth difference between the control and the treatment respectively. Using 2-DE method, a total of 74 differentially expressed protein spots were analyzed using LTQ-FT-ICR MS. Of these proteins, 45 proteins were up-regulated in the treatment group, and 32 proteins were down-regulated. Analysis of LTQ-FI-ICR MS showed that about 50% of the proteins involved in carbohydrate metabolic process, metabolic process, and cellular metabolic compound salvage were affected by stress. Malate dehydrogenase protein and Glyceraldehyde-3-phosphate dehydrogenase protein related to carbohydrate metabolic process increased the level of protein expression in both 3 and 5-leaf stage under waterlogging stress. The increased abundance of these proteins may play an active role in response to waterlogging stress. These results provide new insights into the morphological alteration and modulation of differentially expressed proteins in sorghum cultivar.

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A Study on the Dry Matter Production and Growth Analysis of Zoysia japonica (잔디의 물질생산과 성장해석에 관한 연구)

  • Jin, AHee Sung;Joon Huh
    • The Korean Journal of Ecology
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    • v.9 no.3
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    • pp.161-184
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    • 1986
  • An experiment was made in order to analyze the growth characteristics and productivity of Zoysia japonica under control, mowing and TIBA treatment conditions at the experimental farm of Kyung Hee univ. from May 26 to Oct. 1 in 1985. The field was planned by the randomized block design method and each treatment was given to three plots (2${\times}$2, 4${\times}$4 and 10${\times}$10 cm) and was replicated three times. Each plot was 1$m^2$ for Zoysia japonica. The sampling of each plot was taken once a week after sowing. In order to know the dry matter of total standing crops, kept 9$0^{\circ}C$ and weighted each organ of the plants. Total leaf area of a plant was measured by drawing method. The author adopted the growth analysis of English School. The increasing rates of numbers of leaf and stem were remarkably high in each plot between 10th and 12th weak after sowing and it was highest in TIBA treated plot. The increasin rate of leaf area in each plot was remarkably high beween 10th and 12th week and the maximum value of leaf area was 274.00c$m^2$ in TIBA treated plot of 100 plants/$m^2$. The increasing rate of standing crop was remarkably high between 10th and 12th week and the high increasing tendency in TIBA plot resulted from TIBA. The positive correlationship was founded between standing crop and leaf area. The evaporation rather than temperature acted as a main factor of negative correlation with standing crop during the experiment period. Solar radiation had a high positive correlation in the lower density of plot. C/F ratio was low, during the growth period, from 10th to 12th week after sowing and was low in the higher density under each treated plot. T/R ratio was not constant during the sampling period but was high in the lower density. The increasing rates of RGR and NAR were high between 11th and 12th week after sowing. Leaf area ratio was high in higher density in each plot and not constant in all treated plot.

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Quality Characteristics of Dolsan Leaf Mustard according to Various Blanching Conditions and Liquid Chromatography with Photodiode Array and Tandem Mass Spectrometry Analysis (다양한 데침조건에서 돌산갓의 이화학적 특성 및 LC-PDA/MS/MS 분석)

  • Son, Hae-Reon;Oh, Sun-Kyung;Tsukamoto, Chigen;Choi, Myeong-Rak
    • KSBB Journal
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    • v.31 no.1
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    • pp.20-26
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    • 2016
  • This study aims to examine the optimum blanching conditions as a pretreatment condition to improve the storage stability of Dolsan leaf mustard pickle. The effects of the blan- ching temperature and time were investigated at a temperature range of $80-100^{\circ}C$. Sampling was done for 1 month after a 5 days interval. The L value of the Dolsan leaf mustard was found to be the highest at $80^{\circ}C$. The cutting force increased as the blanching temperature increased. The tensile strength decreased at $95^{\circ}C$ and $100^{\circ}C$. In addition, the sensory evaluation scores were the best at $80^{\circ}C$. The storage stability was assessed at various blanching temperatures to increase the sinigrin content during storage. Liquid chromatography with photodiode array and tandem mass spectrometry (LC-PDA/MS/MS) analysis was conducted to identify and quantify the sinigrin content in the Dolsan leaf mustard. Sinigrin as an internal standard was co-injected into each sample solution. The sample was monitored by recording the ultraviolet absorbance at 228 nm and by electrospray ionization (ESI) positive ion mode in the m/z 50-1,500 range. Blanching the sample at $80^{\circ}C$ showed the highest sinigrin concentration during storage among various temperatures and the maximum concentration was 350 ppm at 15 days storage. Study on utilization of vegetable from food processing of leaf mustard and preservation conservation results suggest that blanching at $80^{\circ}C$ is expected to improve the palatability of the pickle.