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PROMOTER METHYLATION OF THE CDH-13 GENE IN THE ORAL SQUAMOUS CELL CARCINOMA (구강 편평상피암종에서 CDH-13 유전자의 promoter methylation에 대한 연구)

  • Lee, Moon-Joo;Han, Se-Jin;Kim, Kyung-Wook
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.34 no.5
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    • pp.525-531
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    • 2008
  • CDH-13(T-cadherin), which is one of a kind among the 20 cadherins, can be found mainly in wall of aorta, neuron, spleen, blood vessel etc. It is also called H-cadherin. This structural difference can explain that CDH-13 is thought to play a key role in maintaining mutual relation between extra and intra-cellular environment rather than in cell adhesion. The main function of CDH-13 is to participate in blood vessel function. Additionally, it is known to regulate cell growth and cell contact inhibition. When cells are proliferating, cell surface perceives other cells so that substance such as CDH-13 can inhibit their growth or proliferation resulting in homeostasis without endless proliferation or invasion of connective tissue boundaries. However, tumor cell itself appears to be different from normal cells' growth, invasion or transmission. Therefore, it can be diagnosed that these characteristics are closely related to expression of CDH-13 in tumor cells. This study is to investigate expression of CDH-13 in SCC and its correlation with promoter methylation. 20 of tissue species for the study are excised and gathered from 20 patients who are diagnosed as SCC in department of OMS, dental hospital, dankook university. To find development of CDH-13 in each tissue samples, immunohistochemical staining, RT-PCR gene analysis and methylation specific PCR are processed. The results are as follows. 1.Immunohistochemical staining: In normal oral squamous epithelial tissue, strong expression of CDH-13 was found in cell plasma membrane of basal cell layer. On the other hand, in case of low-differentiated oral SCC, development of CDH-13 was hardly seen. 2.The development of CDH-13 gene: In 9 of samples, expression of CDH-13 gene could be seen and 2 of them showed low expression compared to the others. And rest of the 11 samples showed no expression of CDH-13 gene. 3.Methylation of CDH-13 gene: Among 9 samples which expressed CDH-13 gene, 7 of them showed unmethylation. In addition, among 11 samples without CDH-13 gene expression, 10 showed methylation. According to the results stated above, promoter methylation were found in 13 samples(65%) among 20 of oral SCC samples. In low-differentiated SCC, suppression of gene expression could be seen accompanying promoter methylation. These phenomenon of gene expression was proved by immunohistochemical investigation. Finally, for development of oral SCC, conclusions can be made that suppression of CDH-13 played a main role and suppression of gene expression was originated from promoter methylation. Considering this, it is expected that suppression of CDH-13 from promoter methylation to be utilized as a good diagnostic marker of oral SCC.

Fabrication of Small SOFC Stack Based on Anode-Supported Unit Cells and Its Power Generating Characteristics (음극지지형 단전지를 사용한 소형 SOFC 스택의 제조 및 출력특성)

  • Jung, Hwa-Young;Kim, Woo-Sik;Choi, Sun-Hee;Kim, Joosun;Lee, Hae-Weon;Ko, Haengjin;Lee, Ki-Chun;Lee, Jong-Ho
    • Journal of the Korean Ceramic Society
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    • v.41 no.10 s.269
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    • pp.777-782
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    • 2004
  • In this research, $5\times5cm^2$ unit cells were fabricated via liquid condensation process and uniaxial pressing followed by the screen printing of electrolyte and cathode layer. The SOFC stack was assembled with unit cells, gasket-type sealant and metal interconnect. The stack was designed to have a single column with internal-manifold and cross-flow type gas-channels. The SOFC stack produced 15 W, which is $50\%$ of the maximum power being expected from the maximum power density of the unit cell. Controlling factors for the proper operation of the SOFC stack and other designing factors of stack manifold and gas channels were discussed.

A Study on Internal-External Morphology and Pattern Analysis in Angelicae Pubescentis Radix (독활(獨活)의 외부(外部) 및 내부형태(內部形態)와 이화학패턴연구)

  • Kim, Hong-Jun;Kim, Ja-Young;Choi, Go-Ya;Jeong, Seung-Il;Ju, Young-Sung
    • Korean Journal of Oriental Medicine
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    • v.12 no.3 s.18
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    • pp.101-115
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    • 2006
  • The purpose of this study was to introduce the differential standard of the types of Angelicae Pubescentis Radix. We established the classificatory standard according to the external and internal morphology and the physicochemistrical pattern for the four types of Aucklandiae Radix. The results follow: 1. At the external shape, Angelica pubescens f. biserrata has rising lenticel and dark brown surface, Its section is light gray and its xylem is yellow gray. Aralia continentalis has big stem mark in upper portion, its section has no dense tissue with yellow gray. Heracleum hemsleyanum has dark brown surface and little lenticel, its cortical layer is white yellow and xylem is isabella and powdered. 2. At the internal shape, Angelica pubescens f. biserrata has phloem with half of the root and rare vessel. Aralia continentalis has phloem with two-thirds of the root and it has broad cutting and putting vessel that formed 1-2 row. Heracleum hemsleyanum also has phloem with half of the root and wide scattered latex tube and many large vessel. 3. At the TLC pattern, Heracleum hemsleyanum has remarkable dark spot at $R_{f}$ 0.23 on the sulphuric acid color pattern test, but others have faint. 4. At the HPLC pattern, all samples have generally patterns. But Angelica pubescens f. biserrata shows diminutive continentalic acid content and the peak at Rt 20.278min comes out on Heracleum hemsleyanum, but do not come out on Aralia continentalis and Angelica pubescens f. biserrata. Heracleum hemsleyanum has remarkable peak at Rt 20.278min, but shows no peak at Rt 29.023min unlike Aralia continentalis or Angelica pubescens f. biserrata. Also Aralia continentalis and Angelica pubescens f. biserrata show one remarkable peak at Rt 29.023, but Heracleum hemsleyanum do not show. Consequently, Aralia continentalis and Angelica pubescens f. biserrata are comparable whit continentalic acid content and Heracleum hemsleyanum is comparable with the peak at Rt 20.273 and Rt 29.023. So it is thought that content of continentalic acid and the peaks at Rt 20.278 and Rt 29.023 can apply to differentiate a species from other. It is considered the results of this study will be furnished the basis to succeeding studies and it is needed to extensive comparative study for the same genus-degree of relatedness.

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A Study on Improvement of Durability for Run-out Table Roller with Hot Rolling by Porous Self-fluxing Alloy Coating (다공질 자용성 합금 피복에 의한 열간 압연용 런-아웃 테이블 롤러의 내구성 향상에 관한 연구)

  • Bae, Myung-Whan;Park, Byoung-Ho;Jung, Hwa
    • Journal of Advanced Marine Engineering and Technology
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    • v.36 no.2
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    • pp.276-285
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    • 2012
  • The objective of this research is to develop the coating technique by a porous self-fluxing alloy for improving the mechanical properties of run-out table roller surface with the hot rolling. To enhance the durability of run-out table roller with the hot rolling, the high hardness of roller surface should be maintained at high temperatures, and the improvement of wear resistance, corrosion resistance, heat resistance, burn resistance and adhesion resistance should be maintained. In order to be able to transport reliably a hot rolled steel sheet, also, the appropriate friction coefficient on the roller surface should be maintained and the slip between roller and steel should not occur. In this study, the wear resistance of roller increases after the self-fluxing alloy is changed to a cermet by adding the tungsten carbide(WC), and the coefficient of friction increases and the ability of grip is improved because the porosities are made by coating with fine iron powder on the roller surface. As a result, it is found that the ability of grip between the steel and the roller coated by a porous self-fluxing alloy contained to 5 ~ 10 wt% of Fe in the coating layer is improved compared to the roller coated by Ni-Cr. This is because the porosities are made after Fe contained in the roller is partially alloyed by heating with a furnace in the fusing process and the rest is eliminated by oxidation and dissolution.

Contents of Monosaccharides in the Hydrolysates of Some Forest Soil Horizons (삼림토양(森林土壤)의 층위별(層位別) 가수분해물중(加水分解物中) 단당류(單糖類)의 함량(含量))

  • Kim, Jeong-Je;Jang, Yong-Seon;Shin, Young-Oh
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.3
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    • pp.191-196
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    • 1989
  • Monosaccharide content of four forest soils were analyzed. Two soils under coniferous forest trees and another two under duciduous forest trees of Mts. Zeombong and Odae in Kangweon-Do were sampled from the surface horizon down into the subhorizons. 1. The largest amount of monosaccharide is found in the surface organic horizon of each soil and with increasing depth the amount decreases as might be expected considering total organic matter content. 2. Hexoses (galactose, glucose, mannose) predominate over pentoses (arabinose, ribose, xylose) and deoxyhexoses (fucose, rhamnose), the latter being in the smallest amount. Glucose is the most abundant monosaccharide in all samples regardless of vegetation of soil or depth. In general the content of each monosaccharide follows the order of glucose > manrtose > galactose > arabinose > xylose > rhamnose > fucose > ribose. 3. Very little amount of ribose is present even in organic horizons of coniferous forest soils. In samples taken from deciduous forest soils ribose is virtually absent. 4. The relative proportion of monosacchaiide to the total soil organic matter decreases with increasing depth, which may be resulted from the effect of prolonged humification. The total monosaccharide in the organic surface layer amounts to 27-50% of the total organic carbon or 15.7-29% of the total organic matter. Hexoses alone take the largest share of 20-38% of the carbon, or 12-22% of the organic matter.

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Experimental Analysis of a Supersonic Plasma Wind Tunnel Using a Segmented Arc Heater with the Power Level of 0.4 MW (0.4 MW 급 분절형 아크 히터를 이용한 초음속 플라즈마 풍동 특성 실험)

  • Kim, Min-Ho;Lee, Mi-Yeon;Kim, Jeong-Soo;Choi, Chea-Hong;Seo, Jun-Ho;Moon, Se-Yeon;Hong, Bong-Guen
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.41 no.9
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    • pp.700-707
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    • 2013
  • Experimental analyses on a supersonic plasma wind tunnel of CBNU (Chonbuk National University) were carried out. In these experiments, a segmented arc heater was employed as a plasma source and operated at the gas flow rates of 16.3 g/s and the total currents of 300 A. The input power reached ~350 kW with the torch efficiency of 51.4 %, which is defined as the ratio of total exit enthalpy to the input power. The pressure of plasma gas in the arc heater was measured up to 4 bar while it was down to ~45 mbar in a vacuum chamber through a Laval nozzle. During this conversion process, the generated supersonic plasma was expected to have a total enthalpy of ~11 MJ/kg from the measured input power and torch efficiency. In addition to the measurement of total enthalpy, a cone type probe was inserted into the supersonic plasma flow in order to estimate the angle between shock layer and surface of the probe. From these measurements, the temperature and the Mach number of the supersonic plasma were predicted as ~2,950 K and ~3.7, respectively.

Characteristics of Carbonized Biomass Produced in a Manufacturing Process of Wood Charcoal Briquettes Using an Open Hearth Kiln (평로탄화로를 이용한 성형목탄 제조공정에서 생산된 탄화 바이오매스의 특성)

  • JU, Young Min;LEE, Hyung Won;KIM, Ah-ran;JEONG, Hanseob;CHEA, Kwang-Seok;LEE, Jaejung;AHN, Byoung-Jun;LEE, Soo Min
    • Journal of the Korean Wood Science and Technology
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    • v.48 no.2
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    • pp.181-195
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    • 2020
  • Characteristics of carbonized biomass obtained from a Wood charcoal briquette manufacturing process using an open hearth kiln are analyzed in this research, and differences in the characteristics based on the results of a mechanical screening process and the position within the kiln. One type of biomass and five types of carbonized biomass were collected from a Wood charcoal briquette manufacturer. After screening and grinding processes were performed on samples of 1 type of biomass and 5 types of carbonized biomass extracted from a Wood charcoal briquettes manufacturer to classify by particle size, fixed carbon, ash, volatile matters, elemental composition, and high heating value (HHV) were measured. Experimental results showed that the carbonized biomass collected from the middle layer had the highest HHV, 20.4 MJ/kg, and therefore had the highest fuel quality. In terms of particle size, the carbonized biomass below 100 mesh had the lowest ash content and the highest HHV, carbon content, and fixed carbon content. Correlation analyses showed that ash content had negative correlations with HHV, volatile matters, fixed carbon, and carbon content, which suggested that ash content affected negatively on fuel quality.

Effect of Winter Mulching on Growth and Fiber Yield of Ramie Plant (동계피복이 모시풀의 생육 및 섬유수량에 미치는 영향)

  • 권병선;김상곤;정동희;임준택
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.3
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    • pp.208-212
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    • 1993
  • Since ramie suckers distribute soil surface layer within 10cm depth, some of them are dead from low soil temperature in winter. An experiment was conducted to investigate the effects of various mulching methods on safe overwintering of sucker at three or four years old, fully-grown ramie plots. The sprouting dates of ramie was March 28 in transparent vinyl mulching(TPEF), April 1 in transparent vinyl mulching over cover of straw, manure or rice hull at the amount of 500kg /10a (CM), and April 6 in black vinyl mulching(BDEF). These treatments showed earlier sprouting date from 9 to 18 days than the conventional mulching with 1000 kg straw /10a the sprouting date of which was April 15. TPEF treatment showed the earliest but ununiform sprouting. In contrast, sprouts in CM treatment was uniform but emerged somewhat later compared with the treatment of TPEF. The number of stems per unit area increased 50 percent more in TPEF, and 35 to 39 percent more in CM than the treatment of conventional mulching.

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Ultrastructural Study of Vitellogenesis during Oogenesis and Sexual Maturation of the Female Neptunea (Barbitonia) arthritica cumingii on the West Coast of Korea (한국 서해산 암컷 갈색띠매물고둥, Neptunea (Barbitonia) arthritica cumingii의 난자형성과정 중 난황 형성의 미세구조적 연구 및 성 성숙)

  • Chung, Ee-Yung
    • Development and Reproduction
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    • v.9 no.1
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    • pp.23-31
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    • 2005
  • Vitellogenesis during oogenesis, reproductive cycle and first sexual maturity of the female Neptunea (Barbitonia) arthritica cumingii was investigated by light and electron microscope observations. In the early vitellogenic oocyte, the Golgi complex and mitochondria were involved in the formation of lipid droplets and yolk granules. In late vitellogenic oocytes, the rough endoplasmic reticulum and multivesicular bodies were involved in the formation of proteid yolk granules in the cytoplasm. A mature yolk granule was composed of three components: main body(central core), superficial layer, and the limiting membrane. The spawning season was between May and August and the main spawning occurred between June and July when the seawater temperature rose to approximately $18{\sim}23^{\circ}C$. The female reproductive cycle can be classified into five successive stages: early active stage(September to October), late active stage(November to February), ripe stage(February to June), partially spawned stage(May to August), and recovery stage(June to August). The rate of individuals reaching the first sexual maturity was 53.1% in females of 51.0 to 60.9mm in shell height, and 100% in those over 61.0mm.

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Isolation and Characterization of an Agar-hydrolyzing Marine Bacterium, Pseudoalteromonas sp. H9, from the Coastal Seawater of the West Sea, South Korea (서해안 해수로부터 분리한 한천분해 해양미생물 Pseudoalteromonas sp. H9의 동정 및 특성 연구)

  • Chi, Won-Jae;Youn, Young Sang;Kim, Jong-Hee;Hong, Soon-Kwang
    • Microbiology and Biotechnology Letters
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    • v.43 no.2
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    • pp.134-141
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    • 2015
  • An agarolytic marine bacterium (H9) was isolated from the coastal seawater of the West Sea, South Korea. The isolate, H9, was gram-negative and rod-shaped with a smooth surface and polar flagellum. Cells grew at 20-30℃, between pH 5.0 and 9.0, and in ASW-YP (Artificial Sea Water-Yeast extract, Peptone) media containing 1-5% (w/v) NaCl. The G+C content was 41.56 mol%. The predominant isoprenoid quinone in strain H9 was ubiquinone-8. The major fatty acids (>10%) were C16:1ω7c (34.3%), C16:0 (23.72%), and C18:1ω7c (13.64%). Based on 16S rRNA gene sequencing, and biochemical and chemotaxonomic characterization, the strain was designated as Pseudoalteromonas sp. H9 (=KCTC23887). In liquid culture supplemented with 0.2% agar, the cell density and agarase activity reached a maximum level of OD = 4.32 (48 h) and OD = 3.87 (24 h), respectively. The optimum pH and temperature for the extracellular crude agarases of H9 were 7.0 and 40℃, respectively. Thin-layer chromatography analysis of the agarase hydrolysis products revealed that the crude agarases hydrolyze agarose into neoagarotetraose and neoagarohexaose. Therefore, the new agar-degrading strain, H9, can be applicable for the production of valuable neoagarooligosaccharides and for the complete degradation of agar in bio-industries.