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A New Method for Cultivation of Sclerotium of Grifola umbellata

  • Choi, Kyung-Dal;Lee, Kyung-Tae;Shim, Jae-Ouk;Lee, Youn-Su;Lee, Tae-Soo;Lee, Sang-Sun;Guo, Shun-Xing;Lee, Min-Woong
    • Mycobiology
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    • v.31 no.2
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    • pp.105-112
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    • 2003
  • Sclerotia of Grifola umbellata were cultivated by two methods such as burying and root inoculation methods. The sclerotia of G. umbellata produced by the burying method were $6.0{\sim}6.8{\times}3.4{\sim}4.6{\times}1.8{\sim}1.9cm$(Width$\times$Length$\times$Thickness) in size and $17.3{\sim}19.6g$ in weight, respectively. Their increase rate was $1.10{\times}1.12$ times. On the other hand, the sclerotia cultivated by the root inoculation method were $18.3{\sim}31.5{\times}12.5{\sim}26.4{\times}3.1{\sim}3.7cm(W{\times}L{\times}T)$ in size and $219.1{\sim}576.6g$ in weight, respectively. Their growth increment was $11.18{\sim}39.77$ times. The rhizomorphs of Armillaria mellea were developed with a high density under fallen leaves layer covering cultivation site, and distributed mainly between soil surface and soil depth of about 10 cm as well as colonized prominently on the inoculated wood logs. Fungal interaction between G. umbellata and A. mellea were observed mainly in the stage of white sclerotium of G. umbellata. The sclerotia of G. umbellata which were developed newly and harvested in the root inoculation method were twined with root hairs of host tree and rhizomorphs of A. mellea. The sclerotia of G. umbellata decomposing root hairs of host tree were confirmed through SEM examination. Physiochemical characteristics of soil in all cultivation sites had no significant differences. Soil pH were in the range of pH $3.98{\sim}4.40$. Organic matters were the range of $17.97{\sim}23.86%$ and moisture contents of soil were $12.00{\sim}18.20%$. Soil temperatures showed $12.9{\sim}13.8^{\circ}C$ in November and $22.0{\sim}23.9^{\circ}C$ in August, respectively. In conclusion, the root inoculation method seems to be a practical method for cultivating sclerotia of G. umbellata due to its many advantages such as simplicity of inoculation process, shortening of cultivation periods and facility of harvest.

Study on Physical Oceanographic Environments in the Coastal Sea of Chung-Moon, Cheju Island (제주도 중문 연안역의 물리해양환경에 대한 연구)

  • Hong, Chang-Su;Oh, Kyung-Hee;Pang, Ig-Chan
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.6 no.4
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    • pp.211-217
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    • 2001
  • Physical oceanographic environments in the coastal sea of Chung-Moon located in the south coast of Cheju Island, Korea, where water pollutions by growing tourism complex possibly start to influence on the ecological system, are studied with hydrographic data observed monthly during July 1997 to June 2000. Winter and summer characteristics are shown in December to April and June to October, respectively, and transitional characteristics are shown in May and November. Waters show 14{\sim}16^{\circ}C$ and 34${\sim}$34.7 psu in winter and $15{\sim}27^{\circ}C$ and 32${\sim}$34.3 psu in summer. It tells that Tsushima water distributes in the whole column in winter and in the lower layer in summer, and Yangtze coastal water appears in the surface water in summer. When the influence of Yangtze coastal water is strong, salinities below 30psu are shown. Stratification is formed in the depth of about 20 m from June to October, so that it is not shown in the near shore stations, of which the depth is about10 m. Isotherms and isohalines sometimes tend to be perpendicular to the coast line in the surface, which seems to show influences from the steam power plant near St. 1 and the sewage disposal plant near St. 3. During the observation period, temperatures in St. 1 are a little higher than those in St. 2 and St. 3 except for a couple of months in summer and salinities in St. 3 are mostly a little lower than those in St. 1 and St. 2. Their effects seem to be no more than $0.4^{\circ}C$ in a distance of 300 m and no more than 0.1 psu within a distance of 30 m.

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The Design of a Structure of Network Co-processor for SDR(Software Defined Radio) (SDR(Software Defined Radio)에 적합한 네트워크 코프로세서 구조의 설계)

  • Kim, Hyun-Pil;Jeong, Ha-Young;Ham, Dong-Hyeon;Lee, Yong-Surk
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.2A
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    • pp.188-194
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    • 2007
  • In order to become ubiquitous world, the compatibility of wireless machines has become the significant characteristic of a communication terminal. Thus, SDR is the most necessary technology and standard. However, among the environment which has different communication protocol, it's difficult to make a terminal with only hardware using ASIC or SoC. This paper suggests the processor that can accelerate several communication protocol. It can be connected with main-processor, and it is specialized PHY layer of network The C-program that is modeled with the wireless protocol IEEE802.11a and IEEE802.11b which are based on widely used modulation way; OFDM and CDM is compiled with ARM cross compiler and done simulation and profiling with Simplescalar-Arm version. The result of profiling, most operations were Viterbi operations and complex floating point operations. According to this result we suggested a co-processor which can accelerate Viterbi operations and complex floating point operations and added instructions. These instructions are simulated with Simplescalar-Arm version. The result of this simulation, comparing with computing only one ARM core, the operations of Viterbi improved as fast as 4.5 times. And the operations of complex floating point improved as fast as twice. The operations of IEEE802.11a are 3 times faster, and the operations of IEEE802.11b are 1.5 times faster.

Ecological Study of Regenerations in Experimental Canopy Gaps of a Fagus Sylvatica Forest in Germany (독일 너도밤나무(Fagus sylvatica L.) 숲 틈새에서의 갱신생태학적 연구)

  • 변무섭
    • Korean Journal of Environment and Ecology
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    • v.17 no.2
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    • pp.144-152
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    • 2003
  • The purpose of this study was to investigate the effects of lime fertilization and light intensity on beech regeneration strategy in a mature beech forest(ca. 150 years old) gaps, which was located in the soiling areas Germany, where the soil is an acid brown earth. For the experiment canopy gaps of 30m diameter were cut, and one canopy gap remained as untreated while the other was limed(3t dolomite h $a^{-1}$). Soil chemistry, soil water potential, light intensity(PAR), ground vegetation, beech seedlings and mycorrhiza were investigated along transect from the stands into gaps to the north. The results of this study are followings: 1) In the limed gap, soil chemistry was greatly improved and a vigorous herb layer developed as compared to the unlimed gap. 2) Development and growth of the beech seedlings in the unlimed gap were generally deficient. Specially, developments of lateral roots were very lack. 3) Through the lime fertilization, the minerals contents of beech leaves such as calcium(Ca) and magnesium(Mg) were increased, while manganese(Mn) was decreased. There was antagonism between potassium(K) and calcium(Ca) or magnesium(Mg). 4) The relative mycorrhiza frequency(RM) in beech seedlings of the southern edge was very higher than that of the middle part, and through the lime fertilization extension of the species Laccaria amethystina appeared clearly. The results of this study showed that lime fertilize in forest, where the soil is acid, will be necessary..

The Vegetational Characteristics of Bongamsa Forest Genetic Resources Reserve Area in Mt. Heuyang (희양산 봉암사 산림유전자원보호구역 산림군락구조 특성)

  • Lee, Ho-Young;Oh, Choong-Hyeon;Choi, Song-Hyun;Lee, Soo-Dong
    • Korean Journal of Environment and Ecology
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    • v.26 no.3
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    • pp.382-393
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    • 2012
  • The purpose of this study was to research the vegetation structure of the Bongamsa Forest Genetic Resourses Resreve Area in Mt. Heuyang, Mungyeong, Gyeongsangbuk-do. For doing this, ninety-two plots($100m^2/plot$) were set up and investigated, and then Classification analysis and Ordination analysis were carried out. As a result, the vegetation of this area is divided to nine communities; Quercus mongolica community, Quercus variabilis community, Pinus densiflora community, Pinus densiflora-Quercus serrata community, Pinus densiflora-Quercus mongolica community, Quercus serrata community, Decideous broad leaf community, Pinus koraiensis community, Larix kaempferi community. To compare between the communities, statistical analyses were conducted with topographical condition and the results of the vegetational investigation of each community. In altitude, slope, the number of species, the number of individuals in canopy and the number of individuals of understory layer, the mean averages among the communities were different in statistically significance. Then we analysed the vegetation community structure with Importance Percentage of each stratum. The oak tree communities were expected to keep or expand the actual communities because oak trees are spread widely in canopy and understory layers. But the pine tree dominant communities were expected to be succeeded to oak tree communities in the future because of the wide expansion of oak trees.

A Study on the Influence Range of Lateral Movement of Abutment on the Soft Clay by MCC Model (MCC 모델에 의한 연약지반의 교대측방이동 영향범위에 관한 연구)

  • Park, Choon Sik;Kim, Jong Hwan;Baek, Jin Sool
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.1
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    • pp.195-205
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    • 2013
  • This study, using the MCC Model to consider consolidation, estimated the range within which no influences occur from lateral movement and its amount of the foundation pile and abutment on the soft ground. This study performed finite element analyses, with variations on the adhesiveness and internal friction angle, depth of soft clay, embankment height, consolidation parameters, and separation distance between the abutment and embankment. The abutment's horizontal displacement exhibits linear change with a longer separation distance, and changes into an exponential form as the embankment gets closer to the abutment. As the soft clay layer becomes 10 m deeper, the horizontal displacement tends to increase 1.5~3.0 times. However, it decreases at a rate of 0.3~0.95 when adhesiveness is increased by 10 $kN/m^2$ and internal friction angle is increased by $5^{\circ}$. The increase change rate in a lateral movement amount becomes greater if it is closer to the abutment when the abutment separation distance is long. When the distance is short, the change rate of horizontal displacement increases in similar a way, but it tends to be decreasing overall.

Manufacturing Characteristics and Its Color Change of Chewing Gum coated Various Polyols (당알코올로 코팅한 껌의 제조특성과 색택변화에 관한 연구)

  • Lee, Su Han;Lee, Jong Rok;Kim, Jung Hoan
    • Culinary science and hospitality research
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    • v.21 no.6
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    • pp.303-311
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    • 2015
  • This study was carried out to evaluate the possibility of polyols for coating material of chewing gum. Five polyols xylitol, maltitol, isomalt, erythritol, and sorbitol were compared the coating quality, coating and drying time, and color differences. Maltitol was evaluated to be the best quality for coating the gum, whereas erythritol and sorbitol were not considered for coating materials for gum. These results derived from irregular surface layer and low productivity due to increased coating time. According to changes in color of chewing gum, samples coated maltitol and xylitol and isomalt stored at high temperature. In addition, color difference of sample coated maltitol was calculated 2.88 stored at $80^{\circ}C$ for 1 day, but those of xylitol and maltitol were highly evaluated. Sample coated maltitol in polypropylene bag was stored and measured for 1 month. Changes in color of sample was slightly occurred at below $40^{\circ}C$ and the color difference was not more than 3 at $60^{\circ}C$. Chewing gum coated maltitol as coating material was expected more stable in the quality of color during distribution. Current study was performed to color changes during storage, further study will be proceeded about shelf-life of chewing gum coated polyols.

Experimental Study on Heat Flux Partitioning in Subcooled Nucleate Boiling on Vertical Wall (수직 벽면에서 과냉 핵비등 시 열유속 분배에 관한 실험적 연구)

  • Song, Junkyu;Park, Junseok;Jung, Satbyoul;Kim, Hyungdae
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.38 no.6
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    • pp.465-474
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    • 2014
  • To validate the accuracy of the boiling heat flux partitioning model, an experiment was performed to investigate how the wall heat flux is divided into the three heat transfer modes of evaporation, quenching, and single-phase convection during subcooled nucleate boiling on a vertical wall. For the experimental partitioning of the wall heat flux, the wall heat flux and liquid-vapor distributions were simultaneously obtained using synchronized infrared thermometry and the total reflection technique. Boiling experiments of water with subcooling of $10^{\circ}C$ were conducted under atmospheric pressure, and the results obtained at the wall superheat of $12^{\circ}C$ and average heat flux of $283kW/m^2$were analyzed. There was a large difference in the heat flux partitioning results between the experiment and correlation, and the bubble departure diameter and bubble influence factor, which account for a portion of the surrounding superheated liquid layer detached by the departure of a bubble, were found to be important fundamental boiling parameters.

Synthesis and Photovoltaic Properties of a Low Band Gap Polymer for Organic Solar Cell (유기태양전지를 위한 작은 밴드갭 고분자의 합성과 광전특성)

  • Woo, Yong-Ho;Lee, Hyo-Sang;Park, Sungnam;Choi, E-Joon;Kim, BongSoo
    • Polymer(Korea)
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    • v.39 no.1
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    • pp.71-77
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    • 2015
  • We synthesized a low band gap alternating copolymer containing electron-rich units (i.e. dithienosiloles and benzodithiophenes) and electron-deficient units (i.e. difluorobenzothiadiazoles) for high performance organic solar cells. The polymer was prepared by the Stille coupling reaction and characterized using $^1H$ NMR, GPC, TGA, UV-visible absorption spectroscopy, and cyclic voltammetry. Solar cells were fabricated in a structure of ITO/PEDOT:PSS/polymer: $PC_{70}BM/Al$ with five different blending ratios of polymer and $PC_{70}BM$ (1:1.5, 1:2, 1:3, 1:3.5 and 1:4 by weight ratio). The best efficiency was achieved from the 1:3 ratio of polymer and $PC_{70}BM$ in the photoactive layer, and TEM revealed that there is an optimal nanoscale phase separation between polymer and $PC_{70}BM$ in the 1:3 ratio blend film.

Development and Application of Cone Penetrometer with Impact Penetration Rod for Evaluation of Track Substructure (철도궤도 하부구조물 평가를 위한 타격식 관입 롯드가 체결된 콘 관입기의 개발 및 적용)

  • Hong, Wontaek;Byun, Yonghoon;Kim, Sangyeob;Choi, Chanyong;Lee, Jongsub
    • Journal of the Korean GEO-environmental Society
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    • v.16 no.2
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    • pp.45-52
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    • 2015
  • To minimize the cost of maintenance, repair and over-design of track substructure, an accurate evaluation of strength and stiffness of the track substructure is necessary. In this study, a cone penetrometer with impact penetration rod (CPI) is developed for the evaluation of track substructure. For applicability test, the chamber and field tests were performed. As the experimental results of the CPI, dynamic cone penetration endex (DCPI), cone tip resistance ($q_c$), friction resistance ($f_s$) and friction ratio (Fr) were obtained. In the chamber test, the experimental results show reasonable values for the simulated track substructure. In the field test, the CPI clearly detects the interface between the ballast and the subgrade. Also, discontinuous layers are detected in the subgrade. It is expected that the developed CPI may be an effective tool for the evaluation of track substructure by evaluating the ballast layer by dynamic penetration and the subgrade by static penetration of the inner rod.