• 한국응용곤충학회지
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• 제27권1호
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• pp.21-27
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• 1988

인삼 해충, 큰컴정풍뎅이와 참검정풍뎅이의 형태 및 생화사를 구명키 위해 1984-1986년에 걸쳐 야외조사 및 실내실험을 실시하여 다음과 같은 결과를 얻었다. 큰검정충뎅이는 형태적으로 모든 충태에 있어서 유사하였으며, 유충은 두폭과 체장에 있어 큰 검풍뎅이가 다소 컸으나 육안으로 식별할 수 있을 정도는 아니었다. 큰검정풍뎅이는 년 1에 발생하여 산란기는 7월 중순-8월 하순에 9월에는 대부분 종유충인 3형충이 된 후 초동하여 이듬해 5월 하순부터 용화하여 6월 하순에는 우화하였다. 참검정풍뎅 2년에 1에 발생하며 산란기는 5월 하순-7월 상순으로 9월에는 대부분 3영춘이 된후 초동하여 이듬해 4월이면 다시 지표 가까이로 올라와 생식을 계속하다 7월 하순부터 용화, 8월 중순부터 우화하여 성충으로 그대로 추동하였다. 우리 나라에서는 대부분 홀수 해에 비산 및 산란하는 것으로 생각된다. 두 종간에 산란기는 서로 비슷하였으나 유충기간 및 용기간은 참검정풍뎅이가 현저히 길었으며, 특히 3영기간은 55일이다.

• 한국동물학회지
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• 제32권1호
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• pp.1-8
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• 1989

Pieris rapae의 휴면 유도 및 타파기작을 알아보기 위하여 비휴면기 유충과 휴면중인 번데기에 20-HE과 JH을 처리하여 변화를 관찰하는 한편, 체내의 각 호르몬의 농도도 측정하여 보았다. 비휴면기의 종령유충초기에 20-HE과 JH을 주입한 결과 어느 경우에도 휴면이 유도되지 않았다. Pre-diapausing 종령 유충에서는 20-HE 농도가 매우 낮았으며, 비휴면기용 2일의 20-HE peak가 휴면기에서는 나타나지 않았다. JH-I의 농도는 두 시기에 있어 뚜려한 차이가 없었다. 휴면중인 번데기의 초기와 말기에 20-HE, JH-I, JH-III을 주입한 결과 0.1-0.5 $\mu$g의 20-HE에 의해 휴면이 타파되었으나, JH-I과 JH-III에 의한 휴면 타파는 일어나지 않았다. 위의 결과로 배추흰나비의 휴면은 20-HE 농도가 저하됨으로 인해 유도되며, 휴면이 진행되는 동안 20-HE 농도가 점진적으로 증가하여 휴면이 타파되는 것으로 생각된다.

• Applied Microscopy
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• 제12권2호
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• pp.69-79
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• 1982

In order to define the morphological changes of the secreting cells of prothoracic gland during larva-pupal molt, ultrastructural observations were carried out using Bombyx mori L. as the experimental material. At first stage of present experiment, 4 day old 5th instar larva, the polyhedral secreting cells were centrally located in the prothoracic gland surrounded by the connective sheath. The secreting cells were attached to the neighboring cells by the prominent desmosomes, and the plasma membrane contacted with connective sheath were highly infolded. In cytoplasm, the most of the cell organelles, such as rod-like mitochondria, rough surfaced endoplasmic reticulum, ribosome were developed. As the stages advance from larva to pupa, general feature of the secreting cells were retained, but structural changes of the various cytoplasmic organelles-ribosome, rough surfaced endoplasmic reticulum, mitochondria, Golgi apparatus, lamellar body, and vesicle-were noted. In the perinuclear cytoplasm of the secreting cells at the stage of 6 day old 5th instar larva, it is peculiar that only a large amount of ribosomes were distributed and the other organelles were retreated from the juxtanuclear region. Just before and after spining cocoon, these features were more remarkable. Rough surfaced endoplasmic reticulum were gradually increased from the stage just before spining cocoon to the pharate pupa. Rod-like mitochondria with irregular cristae and the matrix showing low density were distributed throughout the cytoplasm in the secreting cells of the 4 day old 5th instar larva. Sometimes, longitudinally distended and curved mitochondria were observed. At the stage of pharate pupa, most of mitochondria were deformed. The rod-like mitochondria of the secreting cells of pupal prothoracic gland were narrower than those of 4 day old 5th instar larva, and the electron density of the mitochondrial matrix is increased in pupa. Golgi apparatus were a few in number in both stages, last instar larva and spining cocoon. In stages of the pharate pupa, the Golgi apparatus were frequently observed. Cytoplasmic vesicles were observed for the first time in the secreting cells of one day after spining cocoon, and the number and the size of cytoplasmic vesicles were distinctly increased inpharate pupa and just after pupation. In the secretory cells of the PG, it in concluded that the RER was closely related to syntheting the enzymes seem to produce the ecdysone.

• 한국응용곤충학회지
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• 제38권3호
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• pp.217-224
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• 1999

두류 품종별 잎특성에 따른 담배거세미나방 유충의 발육과 식엽량을 조사하였다.유충 기간은 검정콩 l호에서 11.5일로 가장 짧았으며, 대광땅콩에서 15.7일로 가장 길어어 두류 품종에 따라 현저한 차이가 있었다. 유충의 영기별 발육기간은 l령에서 3 .2~5.0일로 가장 길였으며, 4령에서 1.0- I.5일로 가장 짧았다. 유충의 식엽량은 영기가 증가함수록 현저하게 증가하여 마지막 6 령기 동안의 식엽량이 유충기간 총 식엽량의 약 55~74%를 차지하였다. 그리고 유충의 성별 식엽량은 수컷보다 암컷에서 많은 경향이었다. 하지만 유충의 사망율과 성비는 두류 품종별 식엽량과는 관련이 없었다.

• Applied Microscopy
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• 제18권2호
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• pp.102-118
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• 1988

This study was carried out to examine in vitro first whether the storage proteins, which the fat bodies of last larvae from Hyphantria cunea secrete into haemolymph, can be uptaked by the fat body cells of prepupa and then how the uptaked storage proteins can be accumulated in the fat body cells, if uptaken. The fat bodies which had been isolated from last instar larvae were cultured in 1 ml of Grace's insect medium containing $50{\mu}l$ of $^{3}H$-leucine (5.0 mCi/mol, Dupont) at $28{\pm}2^{\circ}C$ for 6 hrs. After the homogenates of the cultured fat bodies were centrifuged at 10,000 rpm for 10 minutes, the proteins included in the supernatant were separated by polyacrylamide gel electrophoreses (non-SDS, 6%). The next treatment of the electrophoresed gel was followed by rinsing. A storage protein band of several bands in the rinsed gel was sliced off. With elution of sliced storage protein bands in Tris-glycine buffer, the purification of radioactive storage proteins from fat bodies was finished. After the purified radioactive storage proteins were added in Grace's insect midis containing fat bodies of the prepupae, they were cultured for the randomly following minutes given as 3, 5, 7, 10, 15, 20 and 30 and for the randomly following hours given as 1, 2, 3 and 4 respectively. The double fixations of the cultured fat bodies in aldehyde and $OsO_4$, were followed by preparation of ultrathin sections from Epon-Araldite blocks through dehydration and embedding. The electron microscope autoradiographic treatment of all prepared sections were performed by the dipping method (Kim et al., 1987). The finally prepared specimens were examined with electron microscope. The fat body cells of the prepupa could be found to uptake the storage preteins of the last instar larvae, which were included in the culture medium, mostly by formation of coated vesicles. The in vitro uptake of the storage proteins actively occurred by 30 minutes after the addition of purified storage proteins in the culture medium. After culture for 7 minutes with the storage proteins, the uptaked radioactive storage proteins labelled a number of lysosomal granules. After culture for 20 minutes with the storage proteins, the radioactive storage proteins were finally incorporated and accumulated in lipid droplets and protein granules. The frequency in the fat body cell of radiolabelled lipid droplets occurs approximately 60%, while the frequency, in which the radiolabelled protein granules occurs in a fat body cell, is approximately 40%.

• 한국동물학회지
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• 제36권2호
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• pp.238-244
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• 1993

We identified juvenile hormone binding protein (JHBP) from last instar larval hemollvnph of Hvphantria cunea using gel filtration and non-SDS PAGE. Two kinds of JHBP in hemollnnph were found at two peaks by gel filtration (Sephadex G-100) and also at Rm values of 0.13 and 0.57 by non-SDS PAGE. JHBP was partially purified using anion exchange chromatosraphv, preparative gel filteration, and preparative PAGE. Dextrin coated charcoal (DCC) binding assay was employed to monitor the location of JHBP in chromatographic profile during the purification process. Purity of JHBP was checked by silver staining of 1091 SDS-Polyacrvlamide.

• Applied Microscopy
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• 제26권4호
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• pp.421-430
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• 1996

The endocrine cells in the midgut epithelium of the Japanese cockroach, Periplaneta japonica were observed by the light and electron microscopy. The midgut epithelium of the last instar larva and adult cockroach consisted of principal columnar cells, regenerative cells, and secretory granular cells. Midgut endocrine cells were positioned basally as a cone-shaped single cell in the epithelium or underneath the regenerative crypt cells. When midgut epithelium grows and the cell composing it transforms, between the endocrine cells and regenerative cells were made desmosome type junction and large vesicular shaped stretches of loose contact. The endocrine cells were characterized by a clear cytoplasm with abundant Golgi complex and numerous secretory granules. The secretory granules in the cell were spherical and electron dense with their diameter of $200{\sim}400nm$. The secretory granules have been observed as discharged by exocytosis on the basal and lateral side of the cell.

• Animal cells and systems
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• 제2권3호
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• pp.367-370
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• 1998

Two molecular species of apolipophorin-III (spoLp-III) were purified from the last instar larval hemolymph of Galleria mellonella by gel permeation chromatography (Sephadex G-100), ion exchange chromatography (DE-52), heat treatment (90C for 30 min) and Mono S FPLC, and were named apoLp-III-a and apoLp-lll-b, respectively. They were indistinguishable by SDS-PAGE but could be separated by native PAGE. The molecular mass of apoLp-III determined by SDS-PAGE was approximately 18 kDa. The N-terminal amino acid sequence of apoLp-III-b revealed high similarities with the apoLp-III from Manduca sexta.

• 한국동물학회지
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• 제37권4호
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• pp.495-503
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• 1994

Hemolymph SHBP (hJHBP) was partially purified from last instar larvae of Bombyx zori by gel filtration and their optimal reaction conditions of dextrin coated charcoal binding assay were determined. Dissociation constant (KD) of hJHBP for JH III was calculated to be 1.45 $\times$ 10-7 M at $4^{\circ}C.$ The molecular weight of hJHBP was estimated to be 30 kDa by gel filtration on a calibrated Sephadex G-100 column and 33 kDa by SDS-PAGE. These results indicate that hSHBP consists of a single polvpeptide chain. Isoelectric point of hJHBP was found to be pH 5.1 and 19 of the first 20 amino acid residues were determined from N-terminus of purified hJHBP.

• 한국동물학회지
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• 제34권2호
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• pp.196-202
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• 1991

매미나방 종령유층 혈림프내에 존재하는 JHBP을 Dextran Coated Charcoal (DCC)binding assay와 gel filtration에 의해서 확인하였고, JHBP의 pI값은 5.3으로 밝혀졌다. JHBP의 정저는 혈림프단백질을 먼저 PEG로 침전시킨 후 ion exchange chromatography와 gel filtration 방법을 통하여 행하였다. 정체된 fraction의 JH에 대한 binding activity는 [3H] JH-III의 radioactivity 측정과 DCC binding assay를 통해 확인하였고, 정체된 단백질의 순수도는 각 정체단계에 따라 전지영동을 하여 확인하였다.