• Proceedings of the Optical Society of Korea Conference
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• 2008.02a
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• pp.453-454
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• 2008

femtosecond laser를 광원으로 하는 optical tweezers는 광포획 뿐만 아니라 비선형 현상을 발생시킬 수 있다는 장점을 가지고 있다. 그러나 높은 첨두 출력에 의하여 포획된 세포는 쉽게 손상되어 질 수 있다. 본 논문에서는 LTRS(Laser Tweezers Raman Spectroscopy)를 통하여 femtosecond laser와 CW laser에 의한 optical tweezers 상에서의 optical damage를 비교, 분석하였다.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.1501-1506
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• 2005

This paper presents the design of a multiple beam optical tweezers instrument used for manipulating micro/nano-sized components. The basic equations used in designing the optical tweezers are derived and the stable and time-sharing multiple beam optical tweezers are constructed with scanning mirrors. The laser beam passes through a series of optical components such as lenses, mirrors, and scanning mirrors, and overfills the entrance aperture of microscope objective, which gives a stable trap. By rotating the laser beam with the scanning mirror, the focal positions are translated in the specimen plane and multiple micro/nano-sized objects can be moved. The constructed optical tweezers is used to manipulate cells and liposomes simultaneously and to trap multiple nano-wires. The experiments prove that the developed optical tweezers can be a very versatile manipulation tool for studying gene therapy and nano device fabrication.

• Korean Journal of Computational Design and Engineering
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• v.12 no.4
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• pp.298-307
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• 2007

In recent years, researchers are actively investigating new methods that are applicable for manufacturing micrometer to nanometer scale structures. Among them, optical tweezers that can manipulate microscopic objects using a laser is receiving one of the key attentions. Optical tweezers have been used actively in the field of science. For example, for measuring mechanical characteristics in the scale of piconewtons or for manipulating and sorting large numbers of particles, bacteria, cells. etc. However, little works have been reported for "manufacturing" objects. In this paper, we present a new method for manufacturing micrometer scale structures using micrometer scale biotin coated polystyrene particles. Particles will be controlled with a user interface that utilizes a CyberGlove and glued together by the bonding force between biotin and streptavidin.

• Korean Journal of Optics and Photonics
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• v.19 no.2
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• pp.132-139
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• 2008

In scanning laser optical tweezers, high speed scanning stages are used to manipulate a laser beam spot. Due to the inertia of the stage, the output scanning signal decreases with increased frequency of the input signal. This discrepancy in the signals is difficult to observe since most of the energy from the laser beam is blocked out to avoid CCD damage. In this paper, we propose two methods to alleviate these problems. Firstly, frequency responses of piezo stages are measured to analyze the signal drops and the input signal is compensated accordingly. Secondly, an overlay of the scanning path is drawn on the live monitoring screen to enhance the visibility of the scanning path. The result is a drop-compensated scanning with clear path view.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.29 no.1 s.232
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• pp.110-115
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• 2005

A novel technique for fine particle beam focusing under the atmospheric pressure is introduced using a radiation pressure assisted aerodynamic lens. To introduce the radiation pressure in the aerodynamic focusing system, a 25m plano-convex lens having 2.5mm hole at its center is used as an orifice. The particle beam width is measured for various laser power, particle size, and flow velocity. In addition, the effect of the laser characteristics on the beam focusing is evaluated comparing an optical tweezers type and pure gradient force type. For the pure aerodynamic focusing system, the particle beam width was decreased as increasing particle size and Reynolds number. Using the optical tweezers type, the particle beam width becomes smaller than that of the pure aerodynamic focusing system about $16\%,\;11.4\%\;and\;9.6\%$ for PSL particle size of $2.5{\mu}m,\;1.0{\mu}m,\;and\;0.5{\mu}m$, respectively. Particle beam width was minimized around the laser power of 0.2W. However, as increasing the laser power higher than 0.4W, the particle beam width was increased a little and it approached almost a constant value which is still smaller than that of the pure aerodynamic focusing system. For pure gradient force type, the reduction of the particle beam width was smaller than optical tweezers type but proportional to laser power. The radiation pressure effect on the particle beam width is intensified as Reynolds number decreases or particle size increases relatively.

• Korean Journal of Optics and Photonics
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• v.19 no.1
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• pp.80-83
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• 2008

Optical tweezers are a tool that can use a tightly focused laser beam to trap and manipulate micron-sized dielectric particles that are immersed in a medium with lower refractive index. In this paper, the calculation of the trapping force of optical tweezers is presented. A nonparaxial Gaussian beam is used to represent a tightly focused Gaussian beam, and the FDTD (Finite-Difference Time-Domain) method is used for computing the electromagnetic field distributions in the dielectric medium. Scattered-field formulation is used for analytical expression of the incident fields. Using the electromagnetic field distribution from FDTD simulation, the trapping force is calculated based on Maxwell's stress tensor.

• Proceedings of the Optical Society of Korea Conference
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• 2008.02a
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• pp.237-238
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• 2008

• Journal of the Optical Society of Korea
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• v.11 no.4
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• pp.162-172
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• 2007

Scanning Laser Optical Tweezers(SLOT) is an optical instrument frequently employed on a microscope with laser being delivered through its various ports. In most SLOT systems, a mechanical tilt stage with a mirror on top is used to dynamically move the laser focal point in two-dimensions. The focal point acts as a tweezing spot, trapping nearby microscopic objects. By adding a mechanical translational stage with a lens, SLOT can be expanded to work in three-dimensions. When two mechanical stages operate together, the focal point can address a closed three-dimensional volume that we call a workspace. It would be advantageous to have a large workspace since it means one can trap and work on multiple objects without interruptions, such as translating the microscope stage. However, previous studies have paid less consideration of the volumetric size of the workspace. In this paper, we propose a new method for designing a SLOT such that its workspace is maximized through optimization. The proposed method utilizes a matrix based ray tracing method and genetic algorithm(GA). To demonstrate the performance of the proposed method, experimental results are shown.

• Journal of the Optical Society of Korea
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• v.20 no.1
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• pp.78-87
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• 2016

Red blood cells (RBCs) are customarily adhered to a bio-functionalised substrate to make them stationary in interferometric phase-imaging modalities. This can make them susceptible to receive alterations in innate morphology due to their own weight. Optical tweezers (OTs) often driven by Gaussian profile of a laser beam is an alternative modality to overcome contact-induced perturbation but at the same time a steeply focused laser beam might cause photo-damage. In order to address both the photo-damage and substrate adherence induced perturbations, we were motivated to stabilize the RBC in OTs by utilizing a laser beam of ‘arbitrary intensity profile’ generated by a source having cavity imperfections per se. Thus the immobilized RBC was investigated for phase-imaging with sinusoidal interferograms generated by a compact and robust Michelson interferometer which was designed from a cubic beam splitter having one surface coated with reflective material and another adjacent coplanar surface aligned against a mirror. Reflected interferograms from bilayers membrane of a trapped RBC were recorded and analyzed. Our phase-imaging set-up is limited to work in reflection configuration only because of the availability of an upright microscope. Due to RBC’s membrane being poorly reflective for visible wavelengths, quantitative information in the signal is weak and therefore, the quality of experimental results is limited in comparison to results obtained in transmission mode by various holographic techniques reported elsewhere.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.402.1-402.1
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• 2014

Photonic force microscopy (PFM) is an optical tweezers-based scanning probe microscopy, which measures the forces in the range of fN to pN. The low stiffness leads proper to measure single molecular interaction. We introduce a novel photonic force microscopy to stably map various chemical properties as well as topographic information, utilizing weak molecular bond between probe and object's surface. First, we installed stable optical tweezers instrument, where an IR laser with 1064 nm wavelength was used as trapping source to reduce damage to biological sample. To manipulate trapped material, electric driven two-axis mirrors were used for x, y directional probe scanning and a piezo stage for z directional probe scanning. For resolution test, probe scans with vertical direction repeatedly at the same lateral position, where the vertical resolution is ~25 nm. To obtain the topography of surface which is etched glass, trapped bead scans 3-dimensionally and measures the contact position in each cycle. To acquire the chemical mapping, we design the DNA oligonucleotide pairs combining as a zipping structure, where one is attached at the surface of bead and other is arranged on surface. We measured the rupture force of molecular bonding to investigate chemical properties on the surface with various loading rate. We expect this system can realize a high-resolution multi-functional imaging technique able to acquire topographic map of objects and to distinguish difference of chemical properties between these objects simultaneously.