• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.450-455
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• 2004

A reducing glucose-carrying polymer, called poly [3-O-(4'-vinylbenzyl)-D-glucose］(PVG), was interacted with HepG2 cells including a type-l glucose transporter (GLUT-1) on the cell membrane. The cooperative interaction between a number of GLUT-1s and a number of reducing 3-O-methyl-D-glucose moieties on the PVG polymer chain was found to be responsible for the increase in the interaction with HepG2 cells. The affinity between the cells and the PVG was studied using RITC-labeled glycopolymers. The specific interaction between the GLUT-1 on HepG2 cells and the PVG polymer carrying reducing glucose moieties was suppressed by the inhibitors, phloretin, phloridzin, and cytochalasin B. Direct observation by confocal laser microscopy with the use of RITC-labeled PVG and pretreatment of HepG2 cells with the inhibitors demonstrated that the cells interacted with the soluble form of the PVG polymer via GLUT-1, while fluorescence labeling of the cell surface was prevented after pretreatment with the inhibitors of GLUT-1.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2001.07a
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• pp.644-647
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• 2001

Amorphous As-Ge-Se-S thin films have been studied with the aim of identifying optimum etching condition which can be used to produce holographic grating structure for use as diffractive optical elements. In this study, holographic gratings have been formed using He-Ne laser(632.8nm), and fabricated by the method of wet etching using NaOH etchant with various concentration(0.26N, 0.33N, 0.40N). The diffraction efficiency was obtained by +1st order intensity of the diffracted beam. The formed grating profiles were observed by atomic force microscope and showed that the expected grating profile could be achieved by controlling the etching time. Over-etching resulted in under-cutting of the grating lines. The highest 1st order diffraction efficiency for these gratings was about 5.05%.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.38 no.4
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• pp.195-203
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• 2012

Dental implants using titanium have greatly advanced through the improvement of designs and surface treatments. Nonetheless, the anatomical limits and physiological changes of the patient are still regarded as obstacles in increasing the success rate of implants further, even with the enhancement of implant products. So there have been many efforts to overcome these limits. The intrinsic potential for bone regeneration can be stimulated through adjuvant treatments with the continuous improvement of implant properties, and this can play an important role in achieving optimum osseointegration toward peripheral bone tissue and securing ultimate long-term implant stability in standard surgical procedures. For this purpose, various chemical, biological, or biophysical measures were developed such as bone grafts, materials, pharmacological agents, growth factors, and bone formation proteins. The biophysical stimulation of bone union includes non-invasive and safe methods. In the beginning, it was developed as a method to enhance the healing of fractures, but later evolved into Pulsed Electromagnetic Field, Low-Intensity Pulsed Ultrasound, and Low-Level Laser Therapy. Their beneficial effects were confirmed in many studies. This study sought to examine bone-implant union and its latest trend as well as the biophysical stimulation method to enhance the union. In particular, this study suggested the enhancement of the function of cells and tissues under a disadvantageous bone metabolism environment through such adjunctive stimulation. This study is expected to serve as a treatment guideline for implant-bone union under unfavorable circumstances caused by systemic diseases hampering bone metabolism or the host environment.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2002.04b
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• pp.81-84
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• 2002

The chalcogenide glasses of thin films have the superior property of photoinduced anisotrophy(PA). In this study, we observed the linear dichroism(D) using the irradiation with polarized He-Ne laser light, in the $Ag/As_{10}Ge_{10}Se_{15}S_{35}$ multi-layer. Mutilayer structures formed by alternating metal(Ag) and chalcogenide($As_{10}Ge_{10}Se_{15}S_{35}$). The Ag polarized photodoping result in reducing time of saturation anisotrophy and increasing sensitivity of linearly anisotrophy intensity, up to maximum 220%. In the thin films of chalcogenide, the Ag polarized photodoping will be show a capability of new method that suggested more improvement of photoinduced anisotrophy property.

• Journal of the Korean Society of Visualization
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• v.7 no.1
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• pp.9-13
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• 2009

Fluorescence recovery after photobleaching (FRAP) has been widely used for the measurement of molecular diffusion in living cells and tissues. We developed an image-based FRAP (iFRAP) technique using a modified real-time microscope and a 488 nm Ar-ion laser. A fractional intensity curve was obtained from the time-lapse images of fluorescence recovery in the bleached spot to determine the diffusion coefficient of fluorescently labeled macromolecules in porous medium. We validated iFRAP through experiments with agar gels (0.5% and 1.5% w/v) containing FITC-Dextrans (10, 70 and 500 kDa MW). Further validation was performed by a Monte Carlo approach, where we simulated the three-dimensional random walk of macromolecules in agar gel model. Diffusion coefficients were deduced from the mean square displacement curves and showed good agreements with those measured by iFRAP.

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.4
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• pp.912-919
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• 1994

The engine combustion is one of the most important processes affecting performance and emissions. One effective way to improve the engine combustion is to control the motion of the charge inside a cylinder by means of optimum induction system design, because the flame speed is mainly determined by the turbulence at compression(TDC) process in S.I. engine. It is believed that the tumble and swirl motion generated during intake stroke breaks down into small-scale turbulence in the compression stroke of the cycle. However, the exact nature of this relationship is not well known. This paper describes the tumble flow measurements inside the cylinder of a 4-valve S.I. engine using laser Doppler velocimetry(LDV) under motoring(non-firing) conditions. This is conducted on an optically assesed single cylinder research engine under motored conditions at an engine speed of 1000rpm. Three different cylinder head intake port configurations are studied to develop a better understanding the tumble flow generation, development, and breakdown mechanisms.

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.4
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• pp.920-932
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• 1994

The combustion phenomena of a reciprocating engine is one of the most important processes affecting performance and emissions. One effective way to improve the engine combustion is to control the motion of the charge inside a cylinder by means of optimum induction system design, because the flame speed is mainly determined by the turbulence at compression(TDC) process in S.I. engine. It is believed that the tumble and swirl motion generated during intake breaks down into small-scale turbulence in the compression stroke of the cycle. However, the exact nature of their relationship is not well known. This paper describes cycle resolved LDV measurement of turbulent flow inside the cylinder of a 4-valve engine under motoring(non-firing) conditions, and studies the effect of intake port configurations on the turbulence characteristics using following parameters ; Eulerian temporal autocorrelation coefficient, turbulence energy spectral density function, Taylor micro time scale, integral time scale, and integral length scale.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.25 no.6
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• pp.819-828
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• 2001

The operating point of a small-sized axial fan is strongly dependent upon the system resistance. Therefore, the turbulent flow characteristics around a small-sized axial fan may change significantly according to the operating point. This study represents three-dimensional turbulent flow characteristics around a small-sized axial fan measured at the ideal design point $\phi$=0.25, which is equivalent to the maximum total efficiency point, by using three dimensional fiber-optic type LDA system. This LDA system is composed of a 5 W Argon-ion laser, two optics in back-scatter mode, three BSAs, a PC, and a three-dimensional automatic traversing system. A kind of paraffin fluid is used to supply particles by means of fog generator. Mean velocity profiles downstream of a small-sized axial fan along the radial distance show that the streamwise and the tangential components exist in a predominant manner, while the radial component has a small scale distribution and shows the inflection which its flow direction is inward or outward. Moreover, the turbulent intensity profiles show that the radial component exists the most greatly among turbulent energies.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.33 no.7
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• pp.477-485
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• 2009

The study of nitrogen dilution effect on the flame stability was experimentally investigated in a non-premixed turbulent lifted hydrogen jet with coaxial air. Hydrogen gas was used as a fuel and coaxial air was used to make flame liftoff. Each of hydrogen and air were injected through axisymetric inner and outer nozzles ($d_F=3.65\;mm$ and $d_A=14.1\;mm$). And both fuel jet and coaxial air velocity were fixed as $u_F=200\;m/s$ and $u_A=16\;m/s$, while the mole fraction of nitrogen diluents gas was varied from 0.0 to 0.2 with 0.1 step. For the analysis of flame structure and the flame stabilization mechanism, the simultaneous measurement of PIV/OH PLIF laser diagnostics had been performed. The stabilization point was selected in the most upstream region of the flame base and defined as the point where the turbulent flame propagation velocity was equal to the axial component of local flow velocity. We found that the turbulent flame propagation velocity increased with the decrease of nitrogen mole fraction. We concluded that the turbulent flame propagation velocity was expressed as a function of turbulent intensity and axial strain rate, even though nitrogen diluents mole fraction was changed.

• Bulletin of the Korean Chemical Society
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• v.24 no.2
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• pp.205-208
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• 2003

Laser-induced Raman spectroscopy has been utilized to demonstrate its feasibility for studying the kinetics of imine formation in chloroform solvent. The imine formation, by the nucleophilic addition of primary amine to the carbonyl group of ketone, has been monitored at ten minute intervals for eight hours. The intensity of the C=O stretching mode at 1684 $cm^{-1}$ was measured to determine the rate constant of the reaction. In order to correct the sample-to-sample fluctuations in Raman peak area, this peak was normalized to the C-Cl bending peak at 666 $cm^{-1}$. By the peak area change during the course of reaction, the second order rates at three different temperatures have been determined. The substituent effects on the π conjugations of imine product have also been investigated. On the basis of Raman frequency shifts, the delocalization properties of the aromatic system modified by substitution of a hydrogen atom with -Cl and $-CH_3O$ groups could be clearly understood.