• Journal of the korean society of oceanography
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• v.35 no.3
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• pp.153-157
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• 2000

Four different FITC-conjugated lectins were used to visually evaluate lectin binding activity by optical staining quality using confocal laser scanning microscopy (CLSM) of Cochzodinium polykrikoides in nature (wild type) and culture (cultured type). Cells from the field and cultures treated with ConA fluoresced only at the outer cell wall, and the abundance and distribution of the fluorescent signal were similar. Treatment with PWM and HPA did not elicit fluorescence at the cell surface, but the wild type exposed to HPA showed greater binding than did the cultured cells, possibly due to greater concentrations of glucosamine. The wild type cells treated with LBL lectin showed a strong green fluorescence on the cell surface, whereas cultured cells did not. Signal intensity and abundance were greater than for any other lectins tested in this study. These results suggest that wild type and cultured type are significantly different based on surface sugar production. In particular, the wild type cells apear richer in galactosamine-like moieties. Neither glucose nor mannose-like moieties were present in either wild types or cultured cells.

• Journal of the korean Society of Automotive Engineers
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• v.14 no.3
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• pp.80-89
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• 1992

An experimental study of in-cylinder of flow and combustion characteristics in two gasoline engines of different intake ports which are denoted as original port and masked shroud head (MSH) ports is presented. The flows generated by the MSH and the original port are invest- igated by laser Doppler velocimeter(LDV) under steady flow and motoring (non-firing) condit -ions. Combustion characteristics with different swirl levels produced by two intake ports are analyzed by combustion pressure measurement and statistical calculation. The swirl inside the cylinder of the MSH port engine is found to be much higher than the original port, and the MSH has a large eddy motion of cylinder diameter size. Using ensemble average method to valuate engine turbulence under motoring condition, the MSH port engine is shown to have h -igher turbulence intensity than the original port, so that the effect of the MSH port on fast burn is shown. Also the cyclic variations of peak pressure and the reaching time in the MSH port are apparently reduced.

• Journal of the Korean Applied Science and Technology
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• v.22 no.1
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• pp.77-83
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• 2005

The influence of fluorescence, scattering, and absorbance in turbid material by light scattering was interpreted by the scattered fluorescence intensity and wavelength. The effect of optical property in scattering media was investigated. It is very important to study the charge coupled device(CCD) in spectrometry because we can use the molecular energy level, molecular structure, absorption or emission, intermolecular reaction, weakly bound molecular energy, photochemistry, fluorescence and photodynamic therapy. CCD is very essential to study the molecular structure and medical engineering combined laser spectroscopy in the modem physical and chemistry. Accordingly, this study has designed and manufactured the electromagnetic spectrometry with CCD, and has analyzed the hematoporphyrin derivative.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.13 no.6
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• pp.533-537
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• 2000

It was known that chalcogenide glasses have the superior property of the photoinduced anisotropy(PA). In this study we observed the phenomenon of Ag polarized-photodoping in chalcogenide As$_{40}$ /Ge$_{10}$/Se$_{15}$ /S$_{35}$ and the double-layer of Ag doped As$_{40}$ /Ge$_{10}$/Se$_{15}$ /S$_{35}$ thin film using the irradiation with the polarized He-Ne laser light. The Ag polarized-photodoping results in reducing the time of saturation anisotorpy and increasing the sensitivity of linearly anisotropy intensity up to maximum 220% The Ag polrized-photodoping shows improvement of the photoinduced anisotropy property in polarized photodoping of the chalcogenide thin film. It will offer lots of information for the photodoping mechanism and analysis of chalcogenide thin film.in film.ilm.

• Korean Journal of Optics and Photonics
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• v.11 no.4
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• pp.261-264
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• 2000

Continuous ND filters are fabricated on the silver halide photoplates. These filters enable us to get intensity modulated laser beam. Two kinds of continuous ND filters are fabricated. Optical density of one filter is increased radically and that of the other is decreased. In order to get a filter having desirable optical density, a mask which has reversed optical density has to be made. made.

• Proceedings of the KIEE Conference
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• 1989.07a
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• pp.629-632
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• 1989

The scattered light intensity from a spherical particle passing through the cross-over region of two coherent laser beams, varies periodically. Photodetection of this light beams produces a periodic signal of varying amplitude. The phase of the signal varies with the particle size and refractive index, the beam crossing angle and wavelength, and the position and size of the scattered ligth collecting aperture. In this paper the phase variation with respect to the particle absorptive index of retraction, collecting lens size and beam crossing angle is calculated using both Mie scattering theory and reflection theory. The two theories show good agreement in phase predictions, especially for large absorptive indices and for small collection lenses. Both theories predict phase to be inversely proportional to the beam crossing angle.

• Proceedings of the KIEE Conference
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• 2001.07c
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• pp.1445-1447
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• 2001

We have investigated about the grating formation of the $a-Se_{75}-Ge_{25}$ chalcogenide thin films. In this study, holographic gratings have been formed by using He-Ne laser(632.8nm) with different polarization states(linear, circular polarization). The diffraction efficiency was obtained by +1st order intensity of the diffracted beam. We have obtained maximum efficiency for Ag-doped thin film. It is observed the difference of the diffraction efficiency with polarization states. S:S-polarized state is shown high efficiency than the other polarization.

• Journal of Mechanical Science and Technology
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• v.17 no.1
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• pp.157-167
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• 2003

The exciplex fluorescence technique with the TMPD (tetamethyl-Ρ-phenylene-diamine) / naphthalene dopant system was applied in a combustion-type constant-volume spray chamber. A detailed set of calibration experiments has been performed in order to quantify the TMPD fluorescence signal. It has been demonstrated that the TMPD fluorescence intensity was directly proportional to concentration, was independent of the chamber pressure, and was not sensitive to quenching by either water vapor or carbon dioxide. Using a dual heated-jet experiment, the temperature dependence of TMPD fluorescence up to 1000 K was measured. The temperature field in the spray images was determined using a simple mixing model, and an iterative solution method was used to determine the concentration and temperature field including the additional effects of the laser sheet extinction. The integrated fuel vapor concentration compared favorably with the measured amount of injected fuel when all of the liquid fuel had evaporated.

• Proceedings of the KSME Conference
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• 2002.08a
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• pp.513-516
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• 2002

The flow structure and heat transfer characteristics of a turbulent buoyant jet were investigated experimentally. The instantaneous temperature and velocity fields in the near field were measured using a two-frame PIV and PLIF techniques. A thin light sheet illuminated a two-dimensional cross section of the buoyant jet in which Rhodamine B was added as a fluorescent dye. The intensity variations of LIF signal from Rhodamine B molecules scattered by the laser light were captured by a CCD camera after passing an optical filter. By ensemble averaging the instantaneous temperature and velocity fields, the mean temperature and velocity fields as well as the spatial distributions of turbulent statistics were obtained. The results show the flow structure and convective heat transfer of the developing shear layer in the near field.

• Journal of Mechanical Science and Technology
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• v.16 no.5
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• pp.591-598
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• 2002

Leakage magnetic flux is occurred in the cracked area of magnetized specimens, and also it changes the magnetic domain area of the magneto-optical film positioned on the specimen. It causes the change of the optical permeability of the magnetic domain on the crack area. So crack images can be obtained easily using this principle. On the other hand, utilizing a laser in this method makes possible to perform a remote sensing by detecting the light intensity contrast between cracked area and normal area. This paper shows the application of non-destructive inspection system taking advantage of magneto-optical method for micro-cracks and presents examples applied to the several types of specimens having fatigue cracks and fabricated cracks using this method. Also the authors prove the possibility of this method as a remote sensing system under the oscillation load considering application to real fields.