• Korean Journal of Fisheries and Aquatic Sciences
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• v.21 no.1
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• pp.35-41
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• 1988

In order to set up a predictive model for effective spat collection of sea mussel Mytilus edulis , the survival rate and time required at each developmental stage of drifting larvae were surveyed during the period from March 14 to July 20 in 1987 at the Naesan Ri, Jinhae Bay, the southern part of Korea. The advent of D - shape larvae ca. $120\times90um$ um long had three peaks in that area: April 15, May 13 and June 7. Umbo shape larvae ca.$188\times162{\mu}m$ and full grown larvae ca. $289\times280{\mu}m$ long also showed three peaks: April 27. May 24 and June 20 for the former, and May 10, June 5 and June 30 for the latter. Eleven to thirteen days were required for D - shape larvae to develop to umbo - shape larvae. The instantaneous death rate was 0.1300 and the daily survival rate 0.8781 at this intermorphological stage. The turnover time of umbo to full grown larvae varied from ten to thirteen days with a instantaneous death rate of 0.1520, daily survival rate of 0.8590, and mean survival rate of $16.89\%$. Twenty - three to twenty - five days were required for each group of the D - shape larvae to reach a full grown stage, and their mean survival rate was $3.55\%$ during this developmental period.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.5
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• pp.471-476
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• 1985

In order to set up a predictive model for effective spat collection of Pacific oyster, Crassostrea gigas, the survival rate and time required at each developmental stage of drifting larvae were surveyed during the period from June 13 to October 16 in 1984 at the main oyster farming area, Hansan Bay, the southern part of Korea. The advent of D-shape larvae ca. $72{\times}61{\mu}m$ long had two peaks in that area : July 16 and September 20. Umbo-shape larvae ca. $189{\times}217{\mu}m$ and full grown larvae co. $303{\times}308{\mu}m$ long also showed two peaks: July 21 and September 25 for the former, and July 28 and September 30 for the latter. About five days were required for D-shape larvae to develop to umbo-shape larvae. The instantaneous death rate was 0.24 and the daily survival rate 0.78 with a mean survival rate of $29.38\%$, at this intermorphological stage. The turnover time of umbo to full grown larvae varied from five to seven days with a instantaneous death rate of 0.15, daily survival rate of 0.86 and mean survival rate of $38.54\%$. Ten to twelve days were required for each group of the D-shape larvae to reach a full grown stage, and their mean survival rate was $11.32\%$ during this developmental period.

• Parasites, Hosts and Diseases
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• v.59 no.5
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• pp.519-522
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• 2021

The Chinese edible frogs, Hoplobatrachus rugulosus (n=20), and the striped snakehead fish, Channa striata (n=34), were purchased from local markets in 3 administrative regions of Cambodia (Phnom Penh, Pursat, and Takeo Provinces) from May 2017 to April 2019, and their infection status with Gnathostoma sp. larvae was investigated. The frogs and fish were transported to the laboratory with ice and examined using the artificial digestion method. Advanced 3rd-stage larvae (AdL₃) of Gnathostoma spinigerum, 24 in total number (1-6 larvae/frog), were detected from 6 (60.0%) out of 10 frogs purchased from Phnom Penh. No gnathostome larvae were detected in 10 frogs purchased from Takeo Province and 34 snakeheads from Phnom Penh, Pursat, and Takeo Provinces. AdL₃ isolated from the frogs were 2.55-3.90 mm long and 0.31-0.36 mm wide. They had a characteristic head bulb (0.081×0.191 mm in average size) with 4 rows of hooklets, a muscular long esophagus (0.950-1.230 mm long), and 2 pairs of cervical sacs (0.530-0.890 mm long). The average number of hooklets in the 1st, 2nd, 3rd, and 4th rows was 41, 45, 48, and 51, respectively. These features were consistent with G. spinigerum AdL₃. By the present study, it has been first confirmed that the Chinese edible frog, H. rugulosus, from Phnom Penh serves as a second intermediate host for G. spinigerum, although their intensity of infection was not so high compared to other previously reported localities.

• Proceedings of the Korean Aquaculture Society Conference
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• 2005.05a
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• pp.80-81
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• 2005

To investigate the food organisms of the Acanthogobius hasta during the juveniles stage, the stomach contents of fish, captured in the intertidal zone of Chungchong- nam-do Sochon-gun Su-myon Dodun-ri (Fig. 1) between on early June from end of May 2003, were observed. Total length of the juveniles of A. hasta was 5.5 mm ${\sim}$ 8.9 mm size, and the participation rate of feeding was 69.6%. Main food organisms were such as copepods, decapods, polychaete larvae, amphipods and these occupied more than dry weight 3.9%. Copepods among them dominated the most quantitys by average 62.7%, and next, food organisms appeared much by order of decapods, polychaete larvae and amphipods etc.. Therefore, most important food organisms of juvenile stage of A. hasta were copepods, decapods, polychaete larvae, amphipods etc.

• Proceedings of the Korean Aquaculture Society Conference
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• 2005.05a
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• pp.82-83
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• 2005

To investigate the food organisms of the Chaenogobius mororanus during the juveniles stage, the stomach contents of fish, captured in the intertidal zone of Chungchong- nam-do Sochon-gun Su-myon Dodun-ri (Fig. 1) between on early June from end of May 2003, were observed. Total length of the juveniles of C. mororanus was 5.0 mm ${\sim}$ 8.4 mm size, and the participation rate of feeding was 71.4%. Main food organisms were such as copepods, decapods, polychaete larvae, amphipods and these occupied more than dry weight 3.9%. Copepods among them dominated the most quantitys by average 62.0%, and next, food organisms appeared much by order of decapods, polychaete larvae and amphipods etc.. Therefore, most important food organisms of juvenile stage of C. mororanus were copepods, decapods, polychaete larvae, amphipods etc.

• Korean Journal of Ecology and Environment
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• v.38 no.spc
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• pp.27-30
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• 2005

Diel vertical migration (DVM) and horizontal distribution of Chaoborus flavicans larvae were studied in Lake Fukami-ike (maximum depth 7.75 m, Central Japan). The larvae showed a clear migration in the 4 ${\sim}$ 7 m layer during the day and throughout the water column at night. It should be noted that, while old instar distributed throughout the column during the night. Young instar was found only in the 1 ${\sim}$ 4 m layer. An examination of horizontal distribution revealed that larval stage was clearly related with the depth. It suggests that Chaoborus appear to exist in the deeper area during the larval stage.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.359-369
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• 2011

Twelve species of food microalgae were investigated to clarify the digestion index of Crassostrea gigas larvae using epifluorescence microscopy to choose an appropriate diet for artificial seed production in hatchery. An experiment was conducted using 1 (D shaped stage), 4 (Early umbo stage), 8 (umbo stage) and 12 (Full grown stage) days old larvae. larvae were stocked in 1 L flasks at 5 individuals/mL and fed $10{\times}10^4$ algal cells/mL of each species individually. Prior to larvae were fed for 3 h and then were observed under the microscope to detect ingestion; larvae were then sieved and replaced in 1 L flasks containing filtered seawater and were observed after 3, 5 and 8 h to analyse the digestion index. Values of digestion indices were specific for each alga. No evidence for the ingestion of Thalassiosira weissflogii was evident at all larval development stages tested. Digestion indices of others microalgae were 0.8-99.7% at 4 stage of larval development stages: Chlorella ellipsoidea (0.8-5.4%), Nannochloris oculata (1.4-5.0%), Isochrysis galbana (99.1-99.5%), Pavlova lutheri (99.1-99.5%), I. aff. galbana (99.4-99.5%), Cheatoceros calcitrans (0.0-99.2%), C. gracilis (0.0-99.7%), C. simplex (0.0-95.9%), Phaeodactylum tricornutum (0.0-99.6%), Tetraselmis tetrathele (0.0-99.7%) and Dunaliella tertiolecta (0.0-99.6%), respectively. Therefore, it is assumed that food microalgae showing the high digestion such as I. galbana should be supplied to the early umbo stage larvae, and then after the umbo larval stage, the mixed microalgae with diatoms and light green algae should be supplied to the full grown stage larvae to increase the digestion of their larvae.

• Development and Reproduction
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• v.18 no.4
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• pp.311-319
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• 2014

Newly hatched black porgy larvae (Acanthopagrus schlegeli) swam to the surface, with the mouth and anus still closed and were 1.90-2.11 mm (mean, 2.0 mm) in total length (TL). The larvae were 2.71-2.94 mm TL (mean, 2.82 mm) on day 2 after hatching. At this time, about two-thirds of the yolk was absorbed, the bladder and intestines had formed, and the mouth and anus were open. Total length was 4.32-4.66 mm (mean, 4.45 mm) at the post-larval stage on days 5-6 after hatching, and the yolk and oil globule were almost absorbed. The end of the notochord began to flex, and 6-8 caudal fin rays were visible. The larvae were 15.37-16.1 mm TL (mean, 15.83 mm) at the juvenile stage on days 30-32 after hatching, and the number of rays in all fins was completely revealed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.5
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• pp.489-494
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• 2010

The nutritional demand of oyster larva (Crassostrea gigas) were investigated to determine the optimal culture conditions and improve micro-algae utilization. Changes in nutrients and shell growth were examined in fed and 96-h (48 h in late umbone stage) oysters at four larval stages. Shell growth increased significantly in D shape larvae, regardless of feeding variations. No growth was observed in starved larvae, except in shell length of umbone (to 11.9 ${\mu}m$). Fed larvae showed significant growth in all development stages (P < 0.05). During starvation, lipids were most significantly decreased in all larval stages (by 76.8%, 68.3%, 76.3%, and 40.3%, respectively), followed by protein (41.1%, 31.1%, 33.1%, 16.7%) and nitrogen-free extracts (40.8%, 24.3%, 36.9%, 20.1%), Gross energy (kcal/g) consumption in each larval stage was 49.6%, 35.1%, 39.1%, and 20.4%, respectively. Our results indicate that lipids are the most important energy source during the early larval development stages of C. gigas.

• Animal cells and systems
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• v.12 no.4
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• pp.279-286
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• 2008

Development and distribution of larval Dungeness crab, Cancer magister Dana, 1852 were investigated in southeastern Alaska from late May to mid-September in 2004. Larvae were collected during daylight hours at three inner and two outer Glacier Bay stations at the two different depths in the water column, above and below the thermocline. Larval density decreased dramatically for three larval stages, zoeae I(ZI), zoeae IV, and zoeae V(ZV), but relatively little for zoeae II and zoeae III. ZI predominated at all stations in late May and were collected until late July. Larval stages progressed seasonally from ZI to ZV and density decreased from ZI through ZV. The densities of each zoeal stage at the inner and outer bay stations and at the shallow and deep depths were similar. The density of each larval stage above(shallow) and below(deep) the thermocline and between inner and outer bay stations were not significantly different. The occurrence of larval Dungeness crab is dramatically later than in other parts of the species range, in that larvae appear in abundance beginning in late May. The pattern of spatial distribution of larval stages for the inland waters of Alaska was also markedly different than the patterns reported for Dungeness crab larvae from other parts of the species range, in that the early and intermediate stages occurred within inland waters; from British Columbia to California these larval stages increase in abundance with distance offshore.