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Scopolamine Production in Suspension Cultures of Tumor Calli from Datura metel L. (흰독말풀(Datura metel L.)종양 캘러스의 현탁배양으로부터 Scopolamine 생성)

  • 이수경;윤길영;김용해;양덕조
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.3
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    • pp.203-211
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    • 2000
  • In this study to produce large-scale scopolamine we were examined in the tumor calli of Datura metel L. induced by Agrobacterium tumefaciens $Ery{101}$. The growth and scopolamine contents of tumor calli were higher under light condition than in dark. The optimum condition of growth and scopolamine production were fluence rate of 16 $\mu$mol $m^{-2}s^{-1}$, spectra of red light region and 16 hour light periods on 50 mL SH liquid medium in 4 weeks culture. To increase of the scopolamine contents in tumor calli, the optimum concentration of nitrogen source were 1.8 mM NH$_4$+ and 40 mM NO$_3$. The optimum elicitor concentration for production of scopolamine were 10 mg/L chitosan and 15 mg/L yeast extract. The effect of precursors were good at the concentration of 0.2 mM tropine and 0.3 mM tropic acid, respectively. In order to increase of growth and scopolamine contents. we induced mutant from Datura metel L. tumor callus. Mutants of tumor calli were obtained by 3 Krad, 4 Krad and 6 Krad of ${60}^Cor-ray$. Among them, 3 Krad tumor callus was excellent on the growth and teratoma induction. The 4 Krad tumor callus was negligible for both growth and teratoma induction. But the 6 Krad tumor callus was the best in growth and teratoma induction. The formation of the mutant calli can be enhanced through hormonal combination of 1 mg/L 2,4-dichlorophenoxyacetic acid and 0.5 mg/L benzyladenine. We carry out selection mutant tumor calli for high content tropane alkaloid and suspension cultures for scopolamine production.

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Han River Pollution Studies (한강의 오염도)

  • Choe, Sang
    • 한국해양학회지
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    • v.7 no.1
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    • pp.24-45
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    • 1972
  • The Han River is an important water source in Seoul and neighbouring districts, for public and industrial supply, and for agriculture and fishery. Nowadays, more than six million inhabitants are supplied withe water from this river. The total length of the river is 470km, and has 17 10$\^$9/㎥ an average annual flow. The hydrographic characteristics at Seoul are 653㎥/sec in an average flow, 4,608㎥/sec in the maximum average flow, and 201㎥/sec in the minimum average flow. These are influenced in some degree by snowmelt in early spring, and greatly by the flood during summer. For the pollution problems, the periods of low flow are critical ones. As a rule they occur around the months November through June. Nowadays, most of the sewage from towns and industries is discharged untreated. Apart from domestic and industrial sewages, there are some discharges of mineral matter by mines in the upriver region. In general, water quality of the Han River is kept very clean and healthy until Kwangnaru of the upper region of Seoul. A large pollution, however, is received in the downstream by the domestic and industrial sewages of Seoul. It can be seen that dissolved oxygen, COD and BOD$\sub$5/ diminish markedly, and the intensity of almost every water parameter of the river continues to increase. Comparison of the figures for 1971 derived from a sampling point 40km downstream of Kwangnaru leads to the conclusion that hardness, Ca and Mg were no changed; alkalinity, Si and soluble- Fe were slightly increased; CO$\sub$2/, acidity, Cl, NO$\sub$2/-N, Cu, Zn and Al were increased in 2 and 3 times; total residue, total ignitious residue, COD, BOD$\sub$5/, NH$\sub$4/-N, PO$\sub$4/-P, Mn, Pb and total-Fe were increased in 4 to 7 times; and SO$\sub$4/, particulate-Fe and Cd were increased in 10 to 11 times. On the other hand, coliforms were increased in 650 times; fecal coliforms in 365 times; enterococci and total plate counts in 30 times, respectively. In view points of water quality standards, the down Han River water is now leveling out in Cd, coliforms and fecal coliforms for the agricultural use; in dissolved oxygen and some trace elements (Cu, Zn, Pb and Cd) for the fishery use; in ammonia, COD, BOD$\sub$5/, and Cd for the drinking use.

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Knowledge based Genetic Algorithm for the Prediction of Peptides binding to HLA alleles common in Koreans (지식기반 유전자알고리즘을 이용한 한국인 빈발 HLA 대립유전자에 대한 결합 펩타이드 예측)

  • Cho, Yeon-Jin;Oh, Heung-Bum;Kim, Hyeon-Cheol
    • Journal of Internet Computing and Services
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    • v.13 no.4
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    • pp.45-52
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    • 2012
  • T cells induce immune responses and thereby eliminate infected micro-organisms when peptides from the microbial proteins are bound to HLAs in the host cell surfaces, It is known that the more stable the binding of peptide to HLA is, the stronger the T cell response gets to remove more effectively the source of infection. Accordingly, if peptides (HLA binder) which can be bound stably to a certain HLA are found, those peptieds are utilized to the development of peptide vaccine to prevent infectious diseases or even to cancer. However, HLA is highly polymorphic so that HLA has a large number of alleles with some frequencies even in one population. Therefore, it is very inefficient to find the peptides stably bound to a number of HLAs by testing random possible peptides for all the various alleles frequent in the population. In order to solve this problem, computational methods have recently been developed to predict peptides which are stably bound to a certain HLA. These methods could markedly decrease the number of candidate peptides to be examined by biological experiments. Accordingly, this paper not only introduces a method of machine learning to predict peptides binding to an HLA, but also suggests a new prediction model so called 'knowledge-based genetic algorithm' that has never been tried for HLA binding peptide prediction. Although based on genetic algorithm (GA). it showed more enhanced performance than GA by incorporating expert knowledge in the process of the algorithm. Furthermore, it could extract rules predicting the binding peptide of the HLA alleles common in Koreans.

Evaluation of Water Quality Variation and Sediment of a Shallow Artificial Lake (Lake llgam) in Located the Metropolitan Area (도심의 얕은 인공호인 일감호의 수질변화특성과 퇴적환경의 평가)

  • Kim, Ho-Sub;Ko, Jae-Man;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.36 no.2 s.103
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    • pp.161-171
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    • 2003
  • The present study evaluated water quality variation, limiting nutrient, and sediment of a shallow eutrophic lake (Lake Ilgam) in the metropolitan area from 2000 to 2002. According to annual mean chl.a ($77.2{\pm}36.6\;{\mu}g/l) and TP ($66.6{\pm}20.5\;{\mu}g/l) concentration and trophic state index (>60), Lake llgam was in very eutrophic status. Both inorganic nitrogen ($NH_3-N$ and $NH_3-N$) and phosphorus (SRP) concentrations in the water column increased during winter and spring, but decreased during summer followed by the phytoplankton development. Evidence for phosphorus and nitrogen as being the potential limiting nutrients for phytoplankton growth was supported by the ratio of DIN/DIP (by mass) (${\sim}$835.8), TSI derivations analysis, and algal growth potential bioassay. Based on the results of TSI derivations, strong nutrient limitation by both N and P occurred from September to November when P content in sediment (114.6 mg P/kg) was relatively low compared with the summer. Sediment contained a large amount of nitrogen (TKN: 4,452${\pm}$283.0mg N/kg dry sediment). Phosphorus content in sediment (TP: 313${\pm}$155 mg P/kg) was relatively low with temporal change. P release rate (0.29${\pm}$0.02 mg $m^{-2}$ $day^{-1}$) was high under the aerobic condition at pH 9. These results indicate that the sediment could play an important role as a source of a limiting nutrient, and temporal change of P content in the sediment is closely related with water quality, especially algal biomass change in Lake llgam.

A Study on Solving of Double-layer Pattern Problem in Daejeon Correlator (대전상관기에서 복층패턴 문제의 해결에 관한 연구)

  • Oh, Se-Jin;Roh, Duk-Gyoo;Yeom, Jae-Hwan;Chung, Dong-Kyu;Oh, Chung-Sik;Hwang, Ju-Yeon
    • Journal of the Institute of Convergence Signal Processing
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    • v.16 no.4
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    • pp.162-167
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    • 2015
  • This paper describes the reason and the problem solving for the double-layer pattern of a Daejeon correlator operated in Korea-Japan Correlation Center. When the electric power of an input signal in the correlator is charged small enough to be buried in the noise, it is hard to see a signal with a specific pattern in the input signal, but when the electric power is large, a specific one is reported to be seen. By comparing data from observation with one from software correlator, it was confirmed from the analysis using the AIPS software that the amplitude gain of a source signal was affected about 3%. Therefore, in order to solve the problem of double-layer patterns, we found that a problem in the memory management module responsible for both the data input and the data serialization of the correlator is a cause for the double-layer pattern detected periodically. In other words, while data is serialized and read repeatedly in the memory area assigned to serialize the data from the serialization module, redundant last data is generated and an overlap for the memory allocation is occurred. Therefore, by modifying the program of the FPGA memory sections on serialization module to correct the problem, we confirmed that double-layer pattern is disappeared and correlation results are normally acquired.

Resource Weighted Load Distribution Policy for Effective Transcoding Load Distribution (효과적인 트랜스코딩 부하 분산을 위한 자원 가중치 부하분산 정책)

  • Seo, Dong-Mahn;Lee, Joa-Hyoung;Choi, Myun-Uk;Kim, Yoon;Jung, In-Bum
    • Journal of KIISE:Computing Practices and Letters
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    • v.11 no.5
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    • pp.401-415
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    • 2005
  • Owing to the improved wireless communication technologies, it is possible to provide streaming service of multimedia with PDAs and mobile phones in addition to desktop PCs. Since mobile client devices have low computing power and low network bandwidth due to wireless network, the transcoding technology to adapt media for mobile client devices considering their characteristics is necessary. Transcoding servers transcode the source media to the target media within corresponding grades and provide QoS in real-time. In particular, an effective load balancing policy for transcoding servers is inevitable to support QoS for large scale mobile users. In this paper, the resource weighted load distribution policy is proposed for a fair load balance and a more scalable performance in cluster-based transcoding servers. Our proposed policy is based on the resource weighted table and number of maximum supported users, which are pre-computed for each pre-defined grade. We implement the proposed policy on cluster-based transcoding servers and evaluate its fair load distribution and scalable performance with the number of transcoding servers.

XML Fragmentation for Resource-Efficient Query Processing over XML Fragment Stream (자원 효율적인 XML 조각 스트림 질의 처리를 위한 XML 분할)

  • Kim, Jin;Kang, Hyun-Chul
    • The KIPS Transactions:PartD
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    • v.16D no.1
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    • pp.27-42
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    • 2009
  • In realizing ubiquitous computing, techniques of efficiently using the limited resource at client such as mobile devices are required. With a mobile device with limited amount of memory, the techniques of XML stream query processing should be employed to process queries over a large volume of XML data. Recently, several techniques were proposed which fragment XML documents into XML fragments and stream them for query processing at client. During query processing, there could be great difference in resource usage (query processing time and memory usage) depending on how the source XML documents are fragmented. As such, an efficient fragmentation technique is needed. In this paper, we propose an XML fragmentation technique whereby resource efficiency in query processing at client could be enhanced. For this, we first present a cost model of query processing over XML fragment stream. Then, we propose an algorithm for resource-efficient XML fragmentation. Through implementation and experiments, we showed that our fragmentation technique outperformed previous techniques both in processing time and memory usage. The contribution of this paper is to have made the techniques of query processing over XML fragment stream more feasible for practical use.

Scab (Venturia nashicola) Resistant Pear, "Wonkyo Na-heukseong 2" (배 검은별무늬병 저항성 "원교 나-흑성 2호")

  • Shin, Il-Sheob;Hwang, Hae-Sung;Shin, Yong-Uk;Heo, Seong;Kim, Ki-Hong;Kang, Sam-Seok;Kim, Yoon-Kyeong
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.354-357
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    • 2009
  • "Wonkyo Na-heukseong 2" was selected from a cross between "Kiyomaro", late season European cultivar with highly resistance and "Mansoo", late season Asian cultivar with long storability, large size and low susceptibility to pear scab made in 1997 at the National Institute of Horticultural & Herbal Science of Rural Development Administration in Korea. "Kiyomaro", released cross between "Taiheiyo" and "Bartlett" with scab resistance caused by Venturia nashicola in Japan, with no visual symptoms on any leaves was used as scab resistant source after field investigation and artificial inoculation test during 1997~1999. "Wonkyo Na-heukseong 2" blooms 1 day earlier than "Mansoo" and 3 days later than "Kiyomaro" in 2008. It is strong in tree vigor and upright-spreading in tree habit. It is classified as highly resistant to pear scab as "Kiyomaro" and "Bartlett", and cross-compatible with parental variety and Korean major pear varieties such as "Niitaka" and "Wonwhang". The average optimum harvest time of "Wonkyo Na-heukseong 2" was approximately 180 days after full bloom and it matured about 20 days shorter than parental varieties. The fruit is spindle in shape and yellowish greenish brown in skin color. Average fruit weight was 484 g and soluble solids content was $13.2^{\circ}Brix$. The flesh had medium to high juice and negligible grit. Its fruit was crisp like Asian pear.

Current Status of Hyperspectral Remote Sensing: Principle, Data Processing Techniques, and Applications (초분광 원격탐사의 특성, 처리기법 및 활용 현용)

  • Kim Sun-Hwa;Ma Jung-Rim;Kook Min-Jung;Lee Kyu-Sung
    • Korean Journal of Remote Sensing
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    • v.21 no.4
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    • pp.341-369
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    • 2005
  • Hyperspectral images have emerged as a new and promising remote sensing data that can overcome the limitations of existing optical image data. This study was designed to provide a comprehensive review on definition, data processing methods, and applications of hyperspectral data. Various types of airborne, spaceborne, and field hyperspectral image sensors were surveyed from the available literatures and internet search. To understand the current status of hyperspectral remote sensing technology and research development, we collected several hundreds research papers from international journals (IEEE Transactions on Geoscience and Remote Sensing, International Journal of Remote Sensing, Remote Sensing of Environment and AVIRIS Workshop Proceedings), and categorized them by sensor types, data processing techniques, and applications. Although several hyperspectral sensors have been developing, AVIRIS has been a primary data source that the most hyperspectral remote sensing researches were relied on. Since hyperspectral data have very large data volume with many spectral bands, several data processing techniques that are particularly oriented to hyperspectral data have been developed. Although atmospheric correction, spectral mixture analysis, and spectral feature extraction are among those processing techniques, they are still in experimental stage and need further refinement until the fully operational adaptation. Geology and mineral exploration were major application in early stage of hyperspectral sensing because of the distinct spectral features of rock and minerals that could be easily observed with hyperspectral data. The applications of hyperspectral sensing have been expanding to vegetation, water resources, and military areas where the multispectral sensing was not very effective to extract necessary information.

Thickness Effect of SiOx Layer Inserted between Anti-Reflection Coating and p-n Junction on Potential-Induced Degradation (PID) of PERC Solar Cells (PERC 태양전지에서 반사방지막과 p-n 접합 사이에 삽입된 SiOx 층의 두께가 Potential-Induced Degradation (PID) 저감에 미치는 영향)

  • Jung, Dongwook;Oh, Kyoung-suk;Jang, Eunjin;Chan, Sung-il;Ryu, Sangwoo
    • Journal of the Microelectronics and Packaging Society
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    • v.26 no.3
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    • pp.75-80
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    • 2019
  • Silicon solar cells have been widely used as a most promising renewable energy source due to eco-friendliness and high efficiency. As modules of silicon solar cells are connected in series for a practical electricity generation, a large voltage of 500-1,500 V is applied to the modules inevitably. Potential-induced degradation (PID), a deterioration of the efficiency and maximum power output by the continuously applied high voltage between the module frames and solar cells, has been regarded as the major cause that reduces the lifetime of silicon solar cells. In particular, the migration of the $Na^+$ ions from the front glass into Si through the anti-reflection coating and the accumulation of $Na^+$ ions at stacking faults inside Si have been reported as the reason of PID. In this research, the thickness effect of $SiO_x$ layer that can block the migration of $Na^+$ ions on the reduction of PID is investigated as it is incorporated between anti-reflection coating and p-n junction in p-type PERC solar cells. From the measurement of shunt resistance, efficiency, and maximum power output after the continuous application of 1,000 V for 96 hours, it is revealed that the thickness of $SiO_x$ layer should be larger than 7-8 nm to reduce PID effectively.