• Korean Chemical Engineering Research
• /
• 제46권2호
• /
• pp.222-226
• /
• 2008

나노입자는 생체활성물질을 지능적으로 전달하는 다양한 응용분야를 위해 개발되고 응용되어왔다. 본 연구에서는 락토신과 메타크릴산-메틸메타크릴레이트 공중합체(eudragit L100)가 나노입자로 가공되었다. 이 eudragit 고분자는 락토신을 위산으로부터 보호해주고 장에서 용출되도록 할 수 있다. 아세톤과 pH 7의 버퍼용액을 각각 비용매, 용매로 사용하였을 경우, 가장 작은 입자크기인 290 nm를 얻을 수 있었다. 얻은 입자는 분산제인 carrageenan 존재하에 동결건조를 통해, 응집을 최소화하며 건조 분말로 전환시킬 수 있었다. 락토신과 eudragit을 이용하여 얻은 일차입자는 SEM 관찰을 통해 수십나노미터크기임을 확인할 수 있었다.

• Journal of Dairy Science and Biotechnology
• /
• 제25권2호
• /
• pp.5-10
• /
• 2007

Ultrasonication was employed to prepare solid lipid nanoparticles (SLN) for smart probiotic nanoparticles as a nanofood. The model probiotic material, lactocin from Lactobacillus plantarum (CBT-LP2), was incorporated into SLN. The CBT-LP2 loaded SLN (CBT-LP2-SLN) were spherical in the photograph of scanning electron microscope (SEM). The particle size measured by laser diffraction (LD) was found to be $97.3{\pm}8.2nm$. Zeta potential analyzer suggested the zeta potential of LP-SLN was $-29.36{\pm}3.68$ mV in distilled water. The entrapment efficiency (EE%) was determined with the sephadex gel chromatogram and high-performance liquid chromatogram (HPLC), and up to 90.59% of nanofood was incorporated. Stability evaluation showed relatively long-term stability with only slight particle growth (P>0.05) after storage at room temperature for 4 weeks. Therefore, ultrasonication is demonstrated to be a simple, available and effective method to prepare high quality SLN loaded probiotic material.