• Journal of Yeungnam Medical Science
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• 제5권2호
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• pp.247-254
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• 1988

저자들은 예후가 매우 불량했던 2예의 심근경색증 환자에서 혈청 lactate dehydrogenase isoenzymesd의 extra band($LD_6$)를 경험하였기에 자세한 임상적 경과를 기술하고, 이에 대한 국내 및 국외 문헌의 고찰도 함께 보고하는 바이다.

• 한국환경보건학회지
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• 제21권4호
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• pp.87-95
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• 1995

The concentrations of cadmium, metallothionein(MT), superoxide dismutase(SOD), and lactate dehydrogenase(LDH) were investigated in liver and kidney of rats which were fed the water containing 100 ppm cadmium chloride with basal diet and 5% Agaricus bisporus diet during 16 weeks. Cadmium concentrations in liver and kidney increased during 16 weeks, and there were significantly higher accumulation of cadmium in the kidney than in the liver. The concentrations of MTs in liver and kidney decreased linearly during 16 weeks, but there was no significant difference between control and experimental group. MT concentrations of liver were significantly higher than those of kidney. The superoxide dismutase activities and lactate dehydrogenase activities were not affected by the diet, but there was a significant difference by the duration of administration. These data indicate that the kidney is a major target organ of chronic cadmium poisoning, and suggest that Cd-induced hepatic injury, via release of Cd-MT, may play an important role in the nephrotoxicity. In conclusion, induction of MT occurs in both the liver and the kidney after administration of $CdCl_2$. However, the kidney is less responsive than the liver to the induction of MT by cadmium, which may contribute to making the kidney the target organ of toxicity during chronic Cd exposure.

• 대한한의학회지
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• 제20권1호
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• pp.122-131
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• 1999

This study was carried out to investigate whether water extract of Sagunja-Tang and its composing herbs have the inhibitory effects on cytotoxicity and lipid peroxidation induced by oxidant in rat renal cortical slices. Cytotoxicity was estimated by measuring lactate dehydrogenase (LDH) activity and lipid peroxidation was examined by measuring malondialdehyde (MDA). a product of lipid peroxidation. When rat renal cortical slices were treated with tert-butylhydroperoxicle (t-BHP) of 1 mM and water decocted herbs. LDH release from the slices was inhibited in dose dependent manner at low concentrations of herbs. It shows that herbs can reduce cytotoxicity, but overdose of herbs can be toxic to the slices. And MDA measurements show each herb has its own activities of preventing cytotoxicity from oxidants. So further studies should be followed to make clear the mechanisms of anti-oxidative effects of herbs.

• 한국동물학회지
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• 제16권1호
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• pp.55-65
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• 1973

한국에서 서식하는 뱀과, 살모사과, 및 북살모사과에 속하는 몇종의 파충류에서 각 장기별로 MDH 및 LDH isozyme을 조사하였던 바 MDH는 2개의 isozyme으로 구성되어 있는 점이 동일하였다. 그러나 대륙유혈목이의 MDH isozyme은 이동속도가 빠른 점이 다른 종과 다르다. 뱀과, 살모사과 및 북살모사과의 구성원 각 종에서 4개의 LDH isozyme을 확인할 수 있었다. 근과 간조직의 LDH isozyme은 근본적으로 같은 pattern이었고 $LDH_4$가 없는 것이 뱀과, 살모사과 및 북살모사과의 특징이었다. 한편 심장에서는 2개의 LDH isozyme이 발견되는데 근조직과는 반대되는 pattern 이었다. 위 homogenate에서 나타나는 LDH isozyme은 심장의 pattern과 동일하다고 볼수 있는데 살모사과와 북살모사과에서는 4개의 isozyme이 나타나는 점이 독특하였다. 따라서 실험 동물의 MDH 및 LDH isozyme pattern에서 의미있는 다양성을 발견할 수 없었다.

• Toxicological Research
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• 제14권3호
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• pp.365-370
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• 1998

To evaluate the renal toxicity of the antitumor agent, p-{N,N,-Bis(2-chloroethyl)amino}-4-phenyl acetyl-amino-2,6-piperidinedione(CK-15), rats were treated with CK-15 (acute: 50mg/kg. i.p., single and subacute: 5mg/kg, i.p., daily for 7 days). The changes in the body weight, water consumption, kidney weights and urine volume after and during the treatment were observed. The concentrations of urinary creatinine and portein, the activities of N-acetyl-${\beta}$-D-glucosaminidase (NAG), alanine aminopeptidase (AAP), ${\gamma}$-glutamyl transpeptidase (${\gamma}$-GT) and lactate dehydrogenase (LDH) in 24hr urine were also determined. The body weight, water consumption, and urine volume were decreased after the acute and subacute administration. However the weights of kidney were not changed after the treatments. The excretion of creatinine was significantly decreased 1 day after acute administration but, returned to the control value. In subactute administration, the excretion of creatinine was gradually decreased. However, the protein excretion did not changed in both treatment. Those indicate that CK-15 might decrease the metabolic rate of muscle. THe urinary activities of NAG, AAP, ${\gamma}$-GT, and LDH were significantly affected bythe drug treatment. The urinary activities of NAG, AAP and ${\gamma}$-GT were significantly increased 1 day after the acute administration and then returned to the control value. However, the urinary activities of LDH were not changed in acute treatment. In subacute treatment, although the urinary activities of NAG were not changed, those of AAP and ${\gamma}$-GT were significantly increased 2.3 times at 3 days during the subacute administration. Also the urinary activities of LDH were significantly increased at 7 day after the administration. These results indicate that the high and subacute administration might induce a damage in the kidney cells. Furthermore the present results suggest that the toxic effects of CK-15 might be due to the accumulation of the metabolites.

• Parasites, Hosts and Diseases
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• 제55권6호
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• pp.623-630
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• 2017

Plasmodium lactate dehydrogenase (pLDH) is a strong target antigen for the determination of infection with Plasmodium species specifically. However, a more effective antibody is needed because of the low sensitivity of the current antibody in many immunological diagnostic assays. In this study, recombinant Plasmodium vivax LDH (PvLDH) was experimentally constructed and expressed as a native antigen to develop an effective P. vivax-specific monoclonal antibody (mAb). Two mAbs (2CF5 and 1G10) were tested using ELISA and immunofluorescence assays (IFA), as both demonstrated reactivity against pLDH antigen. Of the 2 antibodies, 2CF5 was not able to detect P. falciparum, suggesting that it might possess P. vivax-specificity. The detection limit for a pair of 2 mAbs-linked sandwich ELISA was 31.3 ng/ml of the recombinant antigen. The P. vivax-specific performance of mAbs-linked ELISA was confirmed by in vitro-cultured P. falciparum and P. vivax-infected patient blood samples. In conclusion, the 2 new antibodies possessed the potential to detect P. vivax and will be useful in immunoassay.

• Asian-Australasian Journal of Animal Sciences
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• 제25권2호
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• pp.189-193
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• 2012

This experiment was conducted to study variations of serum testosterone and seminal characteristics of Markhoz male goats. Blood samples were obtained via jugular vein, and semen was collected by using an artificial vagina from 14 fertile male goats (2-3 years of age), at 15-day intervals starting on 15 July and ending on 30 October 2010 (during breeding and non-breeding season). Semen volume, total sperm (volume${\times}$concentration), live sperm (%), abnormal sperm (%) and semen pH were significantly superior during the late summer and early autumn (breeding season). Variation of sperm density, motility and progressive motility was not significant during the sampling period. The results presented show that the lowest and highest levels of lactate dehydrogenase in the seminal plasma were recorded in late October (2.82 U/ml) and in late August (4.81 U/ml), respectively. Moreover, the study indicated that the serum testosterone concentration was higher during late summer and early autumn (p<0.05) than at any other of sampling period. There were negative correlations between volume and sperm density (-0.135, p<0.05), and positive correlations between volume and percentage live sperm (0.224) and percentage progressive motility (0.194, p<0.01). Sperm density was correlated with live sperm (0.200, p<0.05) and progressive motility (0.202, p<0.01). The correlation between live sperm and progressive motility was 0.554 (p<0.01). Furthermore, the results in this study indicated a significant positive correlation between live sperm and LDH (0.450) and a negative correlation between sperm density and LDH concentration (-0.272) (p<0.01). Significant, but positive correlations were found between sperm motility and LDH (0.542) and testosterone concentration (0.522), respectively (p<0.05). In conclusion, this study demonstrated that the best obtained semen was collected in late summer (during decreasing photoperiod) and early autumn (September and October). This also coincides with the natural breeding season of Markhoz goats in Iran.

• Allergy, Asthma & Respiratory Disease
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• 제6권6호
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• pp.295-302
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• 2018

Purpose: It is thought that Mycoplasma pneumoniae infection is more prevalent and causes more severe pneumonia in school-age children and young adults than in preschool children; however, recent studies suggest that the infection may be underdiagnosed and more severe in preschool children. This study investigated the clinical characteristics of Mycoplasma pneumoniae pneumonia (MPP) and the risk factors of refractory MPP (RMPP) by age. Methods: We retrospectively reviewed the medical records of 353 children admitted due to MPP from January 2015 to December 2016. Demographics, clinical information, laboratory data and radiological findings were collected from all patients in this study. The patients were divided into 2 groups by the age of 6 years. Also, both preschool (< 6 years old) and school-age (${\geq}6$ years old) children were divided into RMPP and non-RMPP patients. Results: Total febrile days, febrile days before admission and the duration of macrolide antibiotic therapy were significantly longer in school-age children than in preschool children. School-age children had significantly greater risk of lobar consolidation (P=0.036), pleural effusion (P=0.001) and extrapulmonary complications (P=0.019). Necrotizing pneumonia and bronchiolitis obliterans tended to occur more frequently in preschool children than in school-age children. In both preschool and school-age children, lactate dehydrogenase (LDH) levels were significantly higher in RMPP patients than in non-RMPP patients. In preschool children, LDH > 722 IU/L (odds ratio [OR], 3.02; 95% confidence interval [CI], 1.44-6.50) and ferritin > 177 ng/mL (OR, 5.38; 95% CI, 1.61-19.49) were significant risk factors for RMPP, while LDH > 645 IU/L (OR, 4.12; 95% CI, 1.64-10.97) and ferritin > 166 ng/mL (OR, 5.51; 95% CI, 1.59-22.32) were so in school-age children. Conclusion: Clinical features of MPP were significantly different between preschool and school-age children. LDH and ferritin may be significant factors of RMPP in preschool and school-age children.

• 대한물리치료과학회지
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• 제27권3호
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• pp.56-66
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• 2020

Background: The purpose of this study was to investigate the effects of pulsed-ultrasound intervention and continued-ultrasound on the PPT (pressure pain threshold), CK (creatine kinase) and LDH (lactate dehydrogenase) recovery of before EIMD (exercise-induced muscle damage). Design: Randomized Controlled Trial. Methods: Thirty subjects who are student in their 20s at a university participated in this study, these subjects were assigned into three groups, a control group (n=10), experiment group I (n=10), and experiment group II (n=10). The subjects in experimental group were intervened by pulsed-ultrasound and continued-ultrasound, while ones on control group weren't by any intervention after induced EIMD. Results: First, In the comparison of the PPT, there were significant variations with the lapse the time in three groups (p<.001) and there was a significant interaction of time and group (p<.001). In the among group comparison, the PPT of experimental group II was significantly larger than those of other groups (p<.01). Second, In the comparison of the CK, there were significant variations with the lapse the time in three groups (p<.001) and there was a significant interaction of time and group (p<.001). In the among group comparison, the CK of experimental group II was significantly smaller than those of other groups (p<.001). Third, In the comparison of the LDH, there were significant variations with the lapse the time in three groups (p<.001) and there was a significant interaction of time and group (p<.001). In the among group comparison, the LDH of experimental group II was significantly smaller than those of other groups (p<.001). Conclusion: The above results revealed that the continued-ultrasound intervention before an exercise had a positive effect of muscle function after EIMD. Therefore we can consider the continued ultrasound as a considerable intervention method to prevent or reduce an exercise injury.

• Preventive Nutrition and Food Science
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• 제6권1호
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• pp.43-50
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• 2001

The effects of cardiac ischemia-reperfusion and $\beta$-adrenergic stimulation on metabolic function and energetics were investigated in Lan gendorff-perfused spontaneously hypertensive rat (SHR) hearts. Sarcoplasmic reticulum {TEX}$Ca^{2+}${/TEX}-dependent ATPase and cardiac lactate dehydrogenase (LDH) are additionally studied. The perfusion medium (1.0 mM {TEX}$Ca^{2+}${/TEX}) contained 5 mM glucose(+5 U/L insulin) and 2 mM pyruvate as substrates. Global ischemia was induced by reducing perfusion pressure of 100 to 40 cm {TEX}$H_{2}${/TEX}O, followed by 20 min reperfusin. Isoproterenol (ISO, 1$\mu$M) was infused for 10 min. Coronary vascular resistance and myocardial oxygen consumption ({TEX}$MVO_{2}${/TEX}) of SHR were increased in parallel with enhanced venous lactate during ischemia and reperfusion compared to those of Sprague Dawley (SD) hearts. Although ischemia-induced increase in venous lactate and combined adenosine plus inosine was abolished, coronary vasodilation produced in SD during reperfusion. In SHR, depressed reactive hyperemia was associated with a fall in cardiac ATP and CrP/Pi ratio and a rise in intracellular lactate/Pyruvate ratio. On the other hand, ISO produced coronary functional hyperemia and an increase in {TEX}$MVO_{2}${/TEX}. However, these responses were less than those in SHR hearts. The ATPase activity of SHR was attenuated in free {TEX}$Ca^{2+}${/TEX} concentrations used under basal condition and with ISO compared to that of SD. Venous lactate output and cardiac LDH activity were augmented in SHR as influenced by ISO. These results demonstrate that coronary reactive and functional hyperemia was dpressed in SHR, which cold be explained by alterations in the cytosolic phosphorylation potential and the cytosolic redox state manipulated by LDH, and by abnormal free calcium handling.