• Title/Summary/Keyword: laboratory and field tests

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Comparison of Two Commercial Antibody Enzyme-Linked Immunosorbent Assays for Detection of Porcine Reproductive Respiratory Syndrome Virus Infection (돼지생식기호흡기증후군(PRRS) 바이러스 감염 항체 검출 ELISA 상용 키트의 정확도 비교)

  • Pak, Son-Il;Lee, Seung-Hwan;Park, Kyung-Ae
    • Journal of Veterinary Clinics
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    • v.33 no.2
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    • pp.102-106
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    • 2016
  • More than 20 years after the first report of porcine reproductive and respiratory syndrome virus (PRRSV) in Korea, the disease is still having major impact on domestic pig health and relevant industries. Although ELISA tests are commonly used by veterinarians to guide herd management, data on diagnostic performance of the test in field settings are very limited. The objective of this study was to evaluate two commercially available PRRSV ELISA (IDEXX PRRS X3 ELISA and Bionote PRRSV ELISA 4.0) to detect antibodies against PRRSV on serum samples. To this end, a total of 1,108 sera were recruited from 35 swine farms located in Gyeonggi province and tested at the Gyeonggi Province Veterinary Service Center. All tests were performed according to the manufacturer's instructions, by laboratory technicians who routinely perform PRRS testing on blood samples. Samples were collected from two sources of swine populations with different PRRS prevalence; 60 samples (5.4%) were originated from breeding farms and the remaining 1,048 samples (94.6%) were from farrow-to-finish farms. We applied Bayesian latent class model (LCM) for two-tests in the two-population when the accuracy of the gold standard is not available. The model estimated that Bionote ELISA was a bit more specific but slightly less sensitive. The estimated sensitivity and specificity of the IDEXX ELISA were 99.8% (95% CI 98.1-100%) and 86.4% (95% CI 81.4-96.5%), respectively. Sensitivity, specificity, positive predictive value and negative predictive value for Bionote kit were 98.7% (95% CI 92.8-100%), 89.8% (95% CI 86.2-93.1%), 93.8% (95% CI 91.5-96.0%), and 97.8% (95% CI 87.1-100%), respectively. Based on the Bayesian 95% credible intervals, the sensitivity and specificity of the two ELISAs were not significantly different each other when assuming that two kits were imperfect, indicating that two kits performed equally well in terms of sensitivity and specificity in our filed setting.

Assessment of Impact-echo Method for Cavity Detection in Dorsal Side of Sewer Pipe (하수관거 배면 공동 탐지를 위한 충격반향법의 적용성 평가)

  • Song, Seokmin;Kim, Hansup;Park, Duhee;Kang, Jaemo;Choi, Changho
    • Journal of the Korean Geotechnical Society
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    • v.32 no.8
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    • pp.5-14
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    • 2016
  • The leakage of water under sewer pipelines is one of main sources of sinkholes in urban areas. We performed laboratory model tests to investigate the presence of cavities using impact-echo method, which is a nondestructive test method. To simulate a concrete sewer pipe, a thin concrete plate was built and placed over container filled with sand. The cavity was modeled as an extruded polystyrene foam box. Two sets of tests were performed, one over sand and the other on cavity. A new impact device was developed to apply a consistent high frequency impact load on the concrete plate, thereby increasing the reliability of the test procedure. The frequency and transient characteristics of the measured reflected waveforms were analyzed via fast Fourier transform and short time Fourier spectrum. It was shown that the shapes of Fourier spectra are very similar to one another, and therefore cannot be used to predict the presence of cavity. A new index, termed resonance duration, is defined to record the time of vibration exceeding a prescribed intensity. The results showed that the resonance duration is a more effective parameter for predicting the presence of a cavity. A value of the resonance period was proposed to estimate the presence of cavity. Further studies using various soil types and field tests are warranted to validate the proposed approach.

An Estimation on the Applicability of Hollow FRP Soil Nailing System (중공식 FRP쏘일네일링 시스템의 적용성 평가)

  • Lee, Hyuk-Jin;Koh, Hyung-Seon;Han, Yong-Hee;Kim, Hong-Taek
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.6C
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    • pp.385-393
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    • 2006
  • Soil nailing is a reinforcement method used for stabilizing excavated walls or slopes. Due to its much advantages such as ease of construction and economical efficiency, use of soil nailing is increased. However, the soil nail has much disadvantages for use in urban area. The soil nail needs to be installed inevitably beyond private land boundary, which causes rent for use. For this reason, removable soil nailing system was developed. However, the removal rate of this system is just about 50¢¦70%. To resolve this problem, the Fiber Reinforced Plastic (FRP) soil nailing system which does not need to be removed and allows for the installation beyond private land, is developed. In this paper, through theoretical and experimental studies in laboratory and field such as prototype tests, pullout tests, we evaluate the stability and behavior characteristics of the FRP soil nailing system. And, numerical analyses using FLAC2D were performed with respect to various soil conditions, where prototype test for excavation wall and pullout tests were carried out. As a result of this study, the FRP soil nailing systems show similar behavior characteristics with those of removable soil nailing system. Finally, considering the serviceability and mechanical stability of FRP soil nailing systems, it is enough to be used as a good alternative of general soil nailing system.

Evaluation of the Performance and Moisture Retaining Ability in Semi-Rigid Pavement (반강성포장의 성능 및 보수성 평가)

  • Park, Tae-Soon
    • International Journal of Highway Engineering
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    • v.10 no.2
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    • pp.69-79
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    • 2008
  • This study presents the test results on the performance and the moisture retaining ability of semi rigid pavement using the moisture retaining grouting. The two kinds of the grouting materials were used for the Laboratory tests. The method of the tests includes the compression(3 hours and 7 days) and flexural strength(7 days) varying the P lot flow values. The test results show that the variation of the P lot value has no great effects on the strength, however, the different strength was found as the different grouting materials were used. The performance of the semi rigid pavement was evaluated varying the air void ration of the base asphalt pavement. The test results show that the flexural strength of the semi rigid pavement increases with increasing the air void of the base asphalt pavement so that the flexural strength of the semi rigid asphalt pavement can be effected by the air void of the base asphalt pavement. The moisture retaining tests were conducted and compared in the field the comparisons were made with the dense grade asphalt pavement and the semi rigid asphalt pavement with and without spraying the water. The difference of the temperature of the semi rigid pavement with the spraying water has recorded $11^{\circ}C$ when it compared with the dense grade asphalt pavement and $4^{\circ}C$, when it compared with the semi rigid pavement without the spraying the water. It can be seen that decrease the temperature of the pavement by the moisture retaining ability from the semi rigid pavement.

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Estimation of design parameters of TBM using punch penetration and Cerchar abrasiveness test (압입시험 및 세르샤 마모시험에 의한 TBM의 설계변수 추정)

  • Jeong, Ho-Young;Lee, Sudeuk;Jeon, Seokwon
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.16 no.2
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    • pp.237-248
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    • 2014
  • Linear cutting test is known to be very effective to determine machine parameters (i.e. thrust force and torque) and to estimate penetration rate of TBM and other operation conditions. Although the linear cutting test has significant advantages, the test is expensive and time-consuming because it requires large size specimen and high load capacity of the testing machine. Therefore, a few empirical prediction models (e.g. CSM, NTNU and QTBM) alternatively adopt laboratory index tests to estimate design parameters of TBM. This study discusses the estimation method of TBM machine parameters and disc cutter consumption using punch penetration test and Cerchar abrasion test of which the researches are rare. The cutter forces and cutter consumption can be estimated by the empirical models derived from the relationship between laboratory test result with field data and linear cutting test data. In addition, the estimation process was programmed through which the design parameters of TBM (e.g. thrust, torque, penetration rate, and cutter consumption) are automatically estimated using laboratory test results.

Oversea & Domestic Case Studies on Excavation Damaged Zone for Deep Geological Repository for Spent Nuclear Fuel (사용후핵연료 심층 처분장을 위한 국내외 굴착손상영역 사례연구)

  • Jeonghwan Yoon;Ki-Bok Min;Sangki Kwon;Myung Kyu Song;Sean Seungwon Lee;Tae Young Ko;Hoyoung Jeong;Youngjin Shin;Jaehoon Jung;Juhyi Yim
    • Tunnel and Underground Space
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    • v.34 no.1
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    • pp.15-27
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    • 2024
  • In this case study, detailed survey of the Excavation Damaged Zone (EDZ) evaluation for the deep geological repository for high level nuclear waste was conducted. Oversea and Domestic case studies were compiled and investigated. EDZ is considered a crucial factor in the performance assessment of spent fuel disposal, leading to numerous studies worldwide aiming to understand the characteristics of the EDZ and quantitatively assessment of its extent through field and laboratory tests at Underground Research Laboratory (URL) sites. To enhance the understanding of EDZ, this study begins with defining and exploring the history of EDZ, compiling factors influencing EDZ, and summarizing the impacts caused by EDZ. Subsequently, an analysis of EDZ and rock properties is performed, followed by presenting generalized outcomes, limitations drawn from previous research, and proposing future research directions.

Proposition Empirical Equations and Application of Artificial Neural Network to the Estimation of Compression Index (압축지수의 추정을 위한 인공신경망 적용과 경험식 제안)

  • 김병탁;김영수;배상근
    • Journal of the Korean Geotechnical Society
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    • v.17 no.6
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    • pp.25-36
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    • 2001
  • The purpose of this paper is to discuss the effects of soil properties such as liquid limit, water content, etc. on the compression index and to propose the empirical equation of compression index far regional clay and to verify the application Back Propagation Neural Network(BPNN). The compression index values obtained from laboratory tests are in the range of 0.01 to 3.06 for clay soils sampled in eleven regions. As the compare with the results of laboratory test and the predicted compression index value from the proposed empirical equations, the results of empirical equations including single soil parameter have a possibility to be overestimated. Also, the results of empirical equations including multiple soil parameters closed to the measured value more than that of empirical equations including single soil parameter, but the standard error for measured value obtained larger than 0.05. For these reasons, the empirical equations including single or multiple soil parameters proposed base on the results of laboratory test and the determination coefficient is up to 0.89. The result of BPNN shows that correlation coefficient and standard error between test and neural network result is larger than 0.925 and smaller than 0.0196, which means high correlativity, respectively. Especially, the estimated result by neural network, using only three parameters such as natural water content, dry unit weight and in-situ void ratio among various factors is available to the estimation of compression index and the correlation coefficient is 0.974. This result verified the possibility that if BPNN use, the compression index can be predicted by the parameters, which obtained from simplex field test.

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An Experimental Study on the Evaluation of Smear Effect Considering In-situ Conditions (현장여건을 고려한 스미어 영향 평가에 관한 실험적 연구)

  • Park, Yeong-Mog
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.8
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    • pp.85-94
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    • 2012
  • Evaluation of the smear effect caused by mandrel penetration into soft ground for a vertical drain installation is very important to predict the consolidation time of soft ground improvement. 30 kinds of laboratory model tests considering in situ conditions were conducted to investigate the formation of a smear zone and the decrease of coefficient of permeability in the disturbed zone. Three types(C(clay):M(silt)=1:1, 0.5:0.5, and 0:1) of reconstituted samples were used for 3 dimensional smear zone test. An experimental study was performed focusing on length of mandrel penetration, mandrel shape and size, earth pressure, and ground condition(unit weight and grain size distributions). Laboratory test results show that the length of mandrel penetration is the most critical factor for the formation of smear zone. As a result, the ratio between diameter of the smear zone($d_s$) and that of mandrel($d_m$) at field using long mandrel becomes larger than conventional $d_s/d_m$. The ratio between $d_s$ and $d_m$ ranges from 1.89 and 2.48 with the sample at C:M=1:0. It was also found that the $d_s/d_m$ value with the round shape of the mandrel is smaller than that of diamond one. The value of $d_s/d_m$ decreased with larger mandrel size, lower unit weight, and higher earth pressure. However, higher silt content led to increase of $d_s/d_m$. The ratio between coefficient of horizontal permeability in the smear zone($k_{hs}$) and that of undisturbed zone($k_{ho}$) ranged from 0.70 to 0.85. The test results imply that factors and values affecting $k_{hs}/k_{ho}$ show similar tendency with $d_s/d_m$.

A Study on the Area of Reinforcement in the Cross Tunnel by Laboratory Model Test (실내모형실험을 통한 교차터널의 보강영역에 관한 연구)

  • Jung, Hyuk Sang;Hong, Jong Ouk;Baeg, Seung In;Chun, Byung Sik
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.32 no.4C
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    • pp.129-137
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    • 2012
  • With the increase in infrastructure needs and tunnel construction, more complicated geometries have come to light, especially in cross tunnel design and construction. The major factors to influence existing tunnels are spacing between tunnels, relative position, size of the new tunnel, construction techniques, topographic and geologic conditions, structure, and alternative construction. In this study, settlement from an excavation for a new tunnel near an existing tunnel, settlement from a change in position of the new tunnel relative to an existing tunnel, and the distribution of deformations of the existing tunnel as a result of excavating the cross-location are analyzed through laboratory model tests. As the results, in condition of the new tunnels go through below the existing tunnel, not only analysed through the standard of the diameter of the tunnel, so it would need to set up to strengthen the field within each side of the 1D, but also determined the part of the cross in the existing and the new tunnel, should implement the reinforcement from the part of new tunnel to the existing tunneling influence of excavation.

Drainage Performance of Various Subsurface Drain Materials- (배수개선공법개발에 관한 연구(I) -각종 지하배수용 암거재료의 배수성능-)

  • 김철회;이근후;유시조;서원명
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.21 no.3
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    • pp.104-120
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    • 1979
  • I. Title of the Study Studies on the Development of Improved Subsurface Drainage Methods. -Drainage Performance of Various Subsurface Drain Materials- II. Object of the Study Studies were carried out to select the drain material having the highest performance of drainage; And to develop the water budget model which is necessary for the planning of the drainage project and the establishment of water management standards in the water-logged paddy field. III. Content and Scope of the Study 1. The experiment was carried out in the laboratory by using a sand tank model. The drainage performance of various drain materials was compared evaluated. 2. A water budget model was established. Various parameters necessary for the model were investigated by analyzing existing data and measured data from the experimental field. The adaptability of the model was evaluated by comparing the estimated values to the field data. IV. Results and Recommendations 1. A corrugated tube enveloped with gravel or mat showed the highest drainage performance among the eight materials submmitted for the experiment. 2. The drainage performance of the long cement tile(50 cm long) was higher than that of the short cement tile(25 cm long). 3. Rice bran was superior to gravel in its' drain performance. 4. No difference was shown between a grave envelope and a P.V.C. wool mat in their performance of drainage. Continues investigation is needed to clarify the envelope performance. 5. All the results described above were obtained from the laboratory tests. A field test is recommended to confirm the results obtained. 6. As a water balance model of a given soil profile, the soil moisture depletion D, could be represented as follows; $$D=\Sigma\limit_{t=1}^{n}(Et-R_{\ell}-I+W_d)..........(17)$$ 7. Among the various empirical formulae for potential evapotranspiration, Penman's formular was best fit to the data observed with the evaporation pans in Jinju area. High degree of positive correlation between Penman;s predicted data and observed data was confirmed. The regression equation was Y=1.4X-22.86, where Y represents evaporation rate from small pan, in mm/100 days, and X represents potential evapotranspiration rate estimated by Penman's formular. The coefficient of correlation was r=0.94.** 8. To estimate evapotranspiration in the field, the consumptive use coefficient, Kc, was introduced. Kc was defined by the function of the characteristics of the crop soil as follows; $Kc=Kco{\cdot}Ka+Ks..........(20)$ where, Kco, Ka ans Ks represents the crop coefficient, the soil moisture coefficient, and the correction coefficient, respectively. The value of Kco and Ka was obtained from the Fig.16 and the Fig.17, respectively. And, if $Kco{\cdot}Ka{\geq}1.0,$ then Ks=0, otherwise, Ks value was estimated by using the relation; $Ks=1-Kco{\cdot}Ka$. 9. Into type formular, $r_t=\frac{R_{24}}{24}(\frac{b}{\sqrt{t}+a})$, was the best fit one to estimate the probable rainfall intensity when daily rainfall and rainfall durations are given as input data, The coefficient a and b are shown on the Table 16. 10. Japanese type formular, $I_t=\frac{b}{\sqrt{t}+a}$, was the best fit one to estimate the probable rainfall intensity when the rainfall duration only was given. The coefficient a and b are shown on the Table 17. 11. Effective rainfall, Re, was estimated by using following relationships; Re=D, if $R-D\geq}0$, otherwise, Re=R. 12. The difference of rainfall amount from soil moisture depletion was considered as the amount of drainage required. In this case, when Wd=O, Equation 24 was used, otherwise two to three days of lag time was considered and correction was made by use of storage coefficient. 13. To evaluate the model, measured data and estimated data was compared, and relative error was computed. 5.5 percent The relative error was 5.5 percent. 14. By considering the water budget in Jinju area, it was shown that the evaporation amount was greater than the rainfall during period of October to March in next year. This was the behind reasonning that the improvement of surface drainage system is needed in Jinju area.

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