• Proceedings of the Korean Geotechical Society Conference
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• 2002.03a
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• pp.445-452
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• 2002

In the field of civil and geotechnical engineering, students conduct a variety of soil tests to fulfill undergraduate soil mechanics course requirement. There is a range of problems in soil laboratory instruction, such as, some students not getting hands on experience of conducting tests because of inadequate number of apparatus, time constraints and inability in exciting students to seriously conduit the experiments, However when these laboratory soil tests are simulated with multimedia interaction ann visualization techniques, the students conceptual understanding of soil mechanics is enhanced. The simulation program for website teaching is a computer based instructional package intended to complement, and potentially replace, some physical testing in a real soil laboratory. The overall aim of this project is to develop an experimental simulation program toward active learning and development of critical thinking skills, including data interpretation, understanding of the precesses and influential factors, and problem solving. Therefore enable students to access website to team experimental procedure at any time or place.

• Korean Journal of Family Medicine
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• v.39 no.6
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• pp.360-363
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• 2018

Background: Acute alcoholic intoxication patients (AAIP) are a common public health problem. The aim of this study was to perform a comprehensive laboratory analysis for these patients to investigate the co-morbid medical problem. Methods: We retrospectively reviewed laboratory findings of AAIP who were transferred to the emergency department (ED) from January 2017 to June 2017. Results: A total of 160 male patients were enrolled. Sixteen patients (16/160, 10.0%) and three patients (3/160, 1.9%) had macrocytic anemia and microcytic anemia, respectively. A total of 33 patients (33/160, 20.6%) showed thrombocytopenia ($<150{\times}10^9/L$). Twelve patients (12/159, 7.5%) showed low serum albumin level (<3.5 g/dL). Three patients (3/160, 1.9%) had chronic kidney disease stages 3-4 based on estimated glomerular filtration rate. Six patients (6/27, 22.2%) had high hemoglobin A1c (HbA1c) level (>7.0%). Positive rates of hepatitis B surface antigen and antiHBs antibody (anti-HBs Ab) were 3.5% (5/141) and 49.0% (68/141), respectively. Conclusion: Patients with AAIP who were transferred to ED had various laboratory abnormalities (anemia, thrombocytopenia, high HbA1c). They had low positive rate of anti-HBs Ab. This might be a public health problem, suggesting the need of hepatitis B virus vaccination program for AAIP. Our data suggest the need of further nationwide studies.

• Nuclear Engineering and Technology
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• v.54 no.5
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• pp.1804-1812
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• 2022

The aim of this paper is to assess the reliability and accuracy of the PSI standard method, used in many previous works, for the quantification of ND uncertainties in the SPERT-III RIA transient, by quantifying the discrepancy between the actual inserted reactivity and the original static reactivity worth and their associated uncertainties. The assessment has shown that the inherent S3K neutron source renormalization scheme, introduced before starting the transient, alters the original static reactivity worth of the transient CR and reduces the associated uncertainty due to the ND perturbation. In order to overcome these limitations, two additional methods have been developed based on CR adjustment. The comparative study performed between the three methods has showed clearly the high sensitivity of the obtained results to the selected approach and pointed out the importance of using the right procedure in order to simulate correctly the effect of ND uncertainties on the overall parameters in a RIA transient. This study has proven that the approach that allows matching the original static reactivity worth and starting the transient from criticality is the most reliable method since it conservatively preserves the effect of the ND uncertainties on the inserted reactivity during a RIA transient.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.6
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• pp.2935-2937
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• 2014

Studies of the causes of cancer, early detection, prevention or treatment need accurate, comprehensive, and timely cancer data. The clinical laboratory provides important cancer information needed for physicians which influence clinical decisions regarding treatment, diagnosis and patient monitoring. Poor communication between health care providers and clinical laboratory personnel can lead to medical errors and wrong decisions in providing cancer care. Because of the key impact of laboratory information on cancer diagnosis and treatment the quality of the tests, lab reports, and appropriate lab management are very important. A laboratory information management system (LIMS) can have an important role in diagnosis, fast and effective access to cancer data, decrease redundancy and costs, and facilitate the integration and collection of data from different types of instruments and systems. In spite of significant advantages LIMS is limited by factors such as problems in adaption to new instruments that may change existing work processes. Applications of intelligent software simultaneously with existing information systems, in addition to remove these restrictions, have important benefits including adding additional non-laboratory-generated information to the reports, facilitating decision making, and improving quality and productivity of cancer care services. Laboratory systems must have flexibility to change and have the capability to develop and benefit from intelligent devices. Intelligent laboratory information management systems need to benefit from informatics tools and latest technologies like open sources. The aim of this commentary is to survey application, opportunities and necessity of intelligent clinical laboratory as a tool to increase cancer care management productivity.

• Clinical and Experimental Pediatrics
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• v.58 no.10
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• pp.369-373
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• 2015

Purpose: In 2004, the American Heart Association (AHA) had published an algorithm for the diagnosis of incomplete Kawasaki disease (KD). The aim of the present study was to investigate characteristics of supplemental laboratory criteria in this algorithm. Methods: We retrospectively examined the medical records of 355 patients with KD who were treated with intravenous immunoglobulin (IVIG) during the acute phase of the disease. Laboratory data were obtained before the initial IVIG administration and up to 10 days after fever onset. In 106 patients, laboratory testing was performed more than twice. Results: The AHA supplemental laboratory criteria were fulfilled in 90 patients (25.4%), and the frequency of laboratory examination (odds ratio [OR], 1.981; 95% confidence interval [CI], 1.391-2.821; P<0.001) was a significant predictor of it. The fulfillment of AHA supplemental laboratory criteria was significantly associated with refractoriness to the initial IVIG administration (OR, 2.388; 95% CI, 1.182-4.826; P=0.013) and dilatation of coronary arteries (OR, 2.776; 95% CI, 1.519-5.074; P=0.001). Conclusion: Repeated laboratory testing increased the rate of fulfillment of the AHA supplemental laboratory criteria in children with KD.

• Nuclear Engineering and Technology
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• v.52 no.5
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• pp.937-946
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• 2020

The aim in the present work is to simulate accident scenarios of AP1000 during the small-break loss-of-coolant accident (SBLOCA) and investigate the performance and behavior of automatic depressurization system (ADS) during accidents by using MIDAC (The Module In-vessel Degradation severe accident Analysis Code). Four types of accidents with different hypothetical conditions were analyzed in this study. The impact on the thermal-hydraulic of the reactor coolant system (RCS), the passive core cooling system and core degradation was researched by comparing these types. The results show that the RCS depressurization becomes faster, the core makeup tanks (CMT) and accumulators (ACC) are activated earlier and the effect of gravity water injection is more obvious along with more ADS valves open. The open of the only ADS1-3 can't stop the core degradation on the basis of the first type of the accident. The open of ADS1-3 has a great impact on the injection time of ACC and CMT. The core can remain intact for a long time and the core degradation can be prevent by the open of ADS-4. The all results are significant and meaningful to understand the performance and behavior of the ADS during the typical SBLOCA.

• Parasites, Hosts and Diseases
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• v.55 no.1
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• pp.15-20
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• 2017

The aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. A combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites of Eimeria stiedae. Protein spots were recognized by the sera of New Zealand rabbits infected artificially with E. stiedae. The proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) analysis in combination with bioinformatics. Approximately 868 protein spots were detected by silver-staining, and a total of 41 immunoreactive protein spots were recognized by anti-E. stiedae sera. Finally, 23 protein spots were successfully identified. The proteins such as heat shock protein 70 and aspartyl protease may have potential as immunodiagnostic or vaccine antigens. The immunoreactive proteins were found to possess a wide range of biological functions. This study is the first to report the proteins recognized by sera of infected rabbits with E. stiedae, which might be helpful in identifying potential targets for vaccine development to control rabbit coccidiosis.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7433-7438
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• 2013

Aim: To identify new biomarkers for NPC diagnosis with an anti-EBV Western blot test kit. Methods: Serum samples from 64 NPC patients and healthy subjects with four specific VCA-IgA/EA-IgA profiles were tested with an anti-EBV Western blot test kit from EUROIMMUN AG. Proteins were quantified with scores of intensity visually assigned to the protein bands. The markers which showed statistical differences between the NPC and non-NPC subjects were further evaluated in another 32 NPC patients and 32 controls in comparison with established biomarkers including VCA-IgA, EA-IgA, EBV-related protein IgG, and EBV DNA. Results: Among the markers screened, EA-D p45-IgG showed a statistically significant difference (p < 0.05) between NPC and non-NPC subjects with VCA-IgA positivy. In 32 VCA-IgA positive NPC patients and 32 control subjects, the diagnostic accuracy of EA-D p45-IgG was 78.1% with a positive predictive value of 77.8% and a negative predictive value of 78.6%. In the verification experiment, the specificity and sensitivity of EA-D p45-IgG were 75.0% and 90.6 %, respectively. Conclusions: EA-D p45-IgG might be a potential biomarker for NPC diagnosis, especially among VCA-IgA positive subjects.

• Journal of Microbiology and Biotechnology
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• v.33 no.2
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• pp.151-166
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• 2023

Yeast extract is a product prepared mainly from waste brewer's yeast, which is rich in nucleotides, proteins, amino acids, sugars and a variety of trace elements, and has the advantages of low production cost and abundant supply of raw material. Consequently, yeast extracts are widely used in various fields as animal feed additives, food flavoring agents and additives, cosmetic supplements, and microbial fermentation media; however, their full potential has not yet been realized. To improve understanding of current research knowledge, this review summarizes the ingredients, production technology, and applications of yeast extracts, and discusses the relationship between their properties and applications. Developmental trends and future prospects of yeast extract are also previewed, with the aim of providing a theoretical basis for the development and expansion of future applications.

• Animal Bioscience
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• v.36 no.6
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• pp.829-839
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• 2023

Objective: The aim of this study was to clone the mRNA sequence of the Acyl-CoA dehydrogenase long chain (ACADL) gene of goats and explore the effect of ACADL on the differentiation of subcutaneous fat cells on this basis. Methods: We obtained the ACADL gene of goats by cloning and used quantitative real-time polymerase chain reaction (qPCR) to detect the ACADL expression patterns of different goat tissues and subcutaneous fat cells at different lipid induction stages. In addition, we transfect intramuscular and subcutaneous adipocytes separately by constructing overexpressed ACADL vectors and synthesizing Si-ACADL; finally, we observed the changes in oil red stained cell levels under the microscope, and qPCR detected changes in mRNA levels. Results: The results showed goat ACADL gene expressed in sebum fat. During adipocyte differentiation, ACADL gradually increased from 0 to 24 h of culture, and decreased. Overexpression of ACADL promoted differentiation of subcutaneous adipocytes in goat and inhibited their differentiation after interference. Conclusion: So, we infer ACADL may have an important role in positive regulating the differentiation process in goat subcutaneous adipocytes. This study will provide basic data for further study of the role of ACADL in goat subcutaneous adipocyte differentiation and lays the foundation for final elucidating of its molecular mechanisms in regulating subcutaneous fat deposition in goats.