• Title/Summary/Keyword: laboratory's safety

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Experimental study on the condensation of sonic steam in the underwater environment

  • Meng, Zhaoming;Zhang, Wei;Liu, Jiazhi;Yan, Ruihao;Shen, Geyu
    • Nuclear Engineering and Technology
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    • v.51 no.4
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    • pp.987-995
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    • 2019
  • Steam jet condensation is of great importance to pressure suppression containment and automatic depressurization system in nuclear power plant. In this paper, the condensation processes of sonic steam jet in a quiescent subcooled pool are recorded and analyzed, more precise understanding are got in direct contact condensation. Experiments are conducted at atmospheric pressure, and the steam is injected into the subcooled water pool through a vertical nozzle with the inner diameter of 10 mm, water temperature in the range of $25-60^{\circ}C$ and mass velocity in the range of $320-1080kg/m^2s$. Richardson number is calculated based on the conservation of momentum for single water jet and its values are in the range of 0.16-2.67. There is no thermal stratification observed in the water pool. Four condensation regimes are observed, including condensation oscillation, contraction, expansion-contraction and double expansion-contraction shapes. A condensation regime map is present based on steam mass velocity and water temperature. The dimensionless steam plume length increase with the increase of steam mass velocity and water temperature, and its values are in the range of 1.4-9.0. Condensation heat transfer coefficient decreases with the increase of steam mass velocity and water temperature, and its values are in the range of $1.44-3.65MW/m^2^{\circ}C$. New more accurate semi-empirical correlations for prediction of the dimensionless steam plume length and condensation heat transfer coefficient are proposed respectively. The discrepancy of predicted plume length is within ${\pm}10%$ for present experimental results and ${\pm}25%$ for previous researchers. The discrepancy of predicted condensation heat transfer coefficient is with ${\pm}12%$.

Safety Assessment of Lactiplantibacillus (formerly Lactobacillus) plantarum Q180

  • Kwon, Yoo Jin;Chun, Byung Hee;Jung, Hye Su;Chu, Jaeryang;Joung, Hyunchae;Park, Sung Yurb;Kim, Byoung Kook;Jeon, Che Ok
    • Journal of Microbiology and Biotechnology
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    • v.31 no.10
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    • pp.1420-1429
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    • 2021
  • The safety of the probiotic strain Q180, which exerts postprandial lipid-lowering effects, was bioinformatically and phenotypically evaluated. The genome of strain Q180 was completely sequenced, and single circular chromosome of 3,197,263 bp without any plasmid was generated. Phylogenetic and related analyses using16S rRNA gene and whole-genome sequences revealed that strain Q180 is a member of Lactiplantibacillus (Lp., formerly Lactobacillus) plantarum. Antimicrobial resistance (AMR) genes were bioinformatically analyzed using all Lp. plantarum genomes available in GenBank, which showed that AMR genes are present differently depending on Lp. plantarum strains. Bioinformatic analysis demonstrated that some mobile genetic elements such as prophages and insertion sequences were identified in the genome of strain Q180, but because they did not contain harmful genes such as AMR genes and virulence factor (VF)- and toxin-related genes, it was suggested that there is no transferability of harmful genes. The minimum inhibition concentrations of seven tested antibiotics suggested by the European Food Safety Authority guidelines were slightly lower than or equal to the microbiological cut-off values for Lp. plantarum. Strain Q180 did not show hemolytic and gelatinase activities and biogenic amine-producing ability. Taken together, this study demonstrated the safety of strain Q180 in terms of absence of AMR genes and VF- and toxin-related genes as a probiotic strain.

Systematizing Information Use to Address Determinants of Health Worker Health in South Africa: A Cross-sectional Mixed Method Study

  • Muzimkhulu Zungu;Annalee Yassi ;Jonathan Ramodike;Kuku Voyi;Karen Lockhart;David Jones;Spo Kgalamono;Nkululeko Thunzi;Jerry Spiegel
    • Safety and Health at Work
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    • v.14 no.4
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    • pp.368-374
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    • 2023
  • Background: Recognizing that access to safe and healthy working conditions is a human right, the World Health Organization (WHO) calls for specific occupational safety and health (OSH) programs for health workers (HWs). The WHO health systems' building blocks, and the International Labour Organization (ILO), highlight the importance of information as part of effective systems. This study examined how OSH stakeholders access, use, and value an occupational health information system (OHIS). Methods: A cross-sectional survey of OSH stakeholders was conducted as part of a larger quasi experimental study in four teaching hospitals. The study hospitals and participants were purposefully selected and data collected using a modified questionnaire with both closed and open-ended questions. Quantitative analysis was conducted and themes identified for qualitative analysis. Ethics approval was provided by the University of Pretoria and University of British Columbia. Results: There were 71 participants comprised of hospital managers, health and safety representatives, trade unions representatives and OSH professionals. At least 42% reported poor accessibility and poor timeliness of OHIS for decision-making. Only 50% had access to computers and 27% reported poor computer skills. When existing, OHIS was poorly organized and needed upgrades, with 85% reporting the need for significant reforms. Only 45% reported use of OHIS for decision-making in their OSH role. Conclusion: Given the gap in access and utilization of information needed to protect worker's rights to a safe and healthy workplace, more attention is warranted to OHIS development and use as well as education and training in South Africa and beyond.

Enhancement of Signal-to-noise Ratio Based on Multiplication Function for Phi-OTDR

  • Li, Meng;Xiong, Xinglong;Zhao, Yifei;Ma, Yuzhao
    • Current Optics and Photonics
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    • v.2 no.5
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    • pp.413-421
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    • 2018
  • We propose a novel methodology based on the multiplication function to improve the signal-to-noise ratio (SNR) for vibration detection in a phi optical time-domain reflectometer system (phi-OTDR). The extreme-mean complementary empirical mode decomposition (ECEMD) is designed to break down the original signal into a set of inherent mode functions (IMFs). The multiplication function in terms of selected IMFs is used to determine a vibration's position. By this method, the SNR of a phi-OTDR system is enhanced by several orders of magnitude. Simulations and experiments applying the method to real data prove the validity of the proposed approach.

The Correlation of Lower Flash Point data with Activity Coefficient Models

  • Ha, Dong-Myeong;Lee, Sungjin
    • International Journal of Safety
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    • v.10 no.1
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    • pp.5-9
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    • 2011
  • Two popular activity coefficient models, Wilson and NRTL equations have been used to correlate the published flash point data on the n-propanol + propionic acid and n-butanol + propionic acid systems through the optimization method. The results of these correlation were compared with the results calculated by Raoult's law. The optimization method were found to be better than those based on the Raoult's law. The optimization method based on the Wilson equation described the published data more effectively than was the case when the optimization method was based upon the NRTL equation.

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Solution for surrounding rock of strain-softening considering confining pressure-dependent Young's modulus and nonlinear dilatancy

  • Liang, Peng;Gao, Yongtao;Zhou, Yu;Zhu, Chun;Sun, Yanhua
    • Geomechanics and Engineering
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    • v.22 no.4
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    • pp.277-290
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    • 2020
  • This paper presents an elastic-plastic solution for the circular tunnel of elastic-strain softening behavior considering the pressure-dependent Young's modulus and the nonlinear dilatancy. The proposed solution is verified by the results of the field measuring and numerical simulation from a practical project, and a published closed-form analysis solution. The influence of each factor is discussed in detail, and the ability of Young's modulus and dilatancy characterizing the mechanical response of surrounding rock is investigated. It is found that, in low levels of support pressure, adopting the constant Young's modulus model will seriously misestimate the surrounding rock deformation. Using the constant dilatancy model will underestimate the surrounding rock deformation. When adopting the constant dilatancy model, as the dilation angle increases, the range of the plastic region increases, and the surrounding rock deformation weakens. When adopting the nonlinear dilatancy, the plastic region range and the surrounding rock deformation are the largest. The surrounding rock deformation using pressure-dependent Young's modulus model is between those resulted from two constant Young's modulus models. The constant α of pressuredependent Young's modulus model is the main factor affecting the tunnel displacement. The influence of α using a constant dilatancy model is much more apparent than that using a nonlinear dilatancy model.

Experimental study on steel-concrete composite beams with Uplift-restricted and slip-permitted screw-type (URSP-S) connectors

  • Duan, Linli;Chen, Hongbing;Nie, Xin;Han, Sanwei
    • Steel and Composite Structures
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    • v.35 no.2
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    • pp.261-278
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    • 2020
  • In steel-concrete composite beams, to improve the cracking resistance of the concrete slab in the hogging moment region, a new type of connector in the interface, named uplift-restricted and slip-permitted screw-type (URSP-S) connector has been proposed. This paper focuses on the behavior of steel-concrete composite beams with URSP-S connectors. A total of three beam specimens including a simply supported beam with URSP-S connectors and two continuous composite beams with different connectors arrangements were designed and tested. More specifically, one continuous composite beam was equipped with URSP-S connectors in negative moment region and traditional shear studs in other regions. For comparison, the other one was designed with only traditional shear studs. The failure modes, crack evolution process, ultimate capacities, strain responses at different locations as well as the interface slip of the three tested specimens were measured and evaluated in-depth. Based on the experimental study, the research findings indicate that the larger slip deformation is allowed while using URSP-S connectors. Meanwhile, the tensile stress reduces and the cracking resistance of the concrete slab improves accordingly. In addition, the overall stiffness and strength of the composite beam become slightly lower than those of the composite beam using traditional shear studs. Moreover, the arrangement suggestion of URSP-S connectors in the composite beam is discussed in this paper for its practical design and application.

Cryopreservation of Siberian tiger (Panthera tigris altaica) epididymal spermatozoa: pilot study of post-thaw sperm characteristics

  • Ibrahim, Saddah;Talha, Nabeel Abdelbagi Hamad;Kim, Jeongho;Jeon, Yubeol;Yu, Iljeoung
    • Journal of Animal Reproduction and Biotechnology
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    • v.37 no.2
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    • pp.130-135
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    • 2022
  • Epididymal sperm cryopreservation provides a potential method for preserving genetic material from males of endangered species. This pilot study was conducted to develop a freezing method for tiger epididymal sperm. We evaluated post-thaw sperm condition using testes with intact epididymides obtained from a Siberian tiger (Panthera tigris altaica) after castration. The epididymis was chopped in Tyrode's albumin-lactate-pyruvate 1x and incubated at 5% CO2, 95% air for 10 min. The Percoll separation density gradient method was used for selective recovery of motile spermatozoa after sperm collection using a cell strainer. The spermatozoa were diluted with modified Norwegian extender supplemented with 20 mM trehalose (extender 1) and subsequent extender 2 (extender 1 with 10% glycerol) and frozen using LN2 vapor. After thawing at 37℃ for 25 s, Isolate® solution was used for more effective recovery of live sperm. Sperm motility (computerized assisted sperm analysis, CASA), viability (SYBR-14 and Propidium Iodide) and acrosome integrity (Pisum sativum agglutinin with FITC) were evaluated. The motility of tiger epididymal spermatozoa was 40.1 ± 2.0%, and progressively motile sperm comprised 32.7 ± 2.3%. Viability was 56.3 ± 1.6% and acrosome integrity was 62.3 ± 4.4%. Cryopreservation of tiger epididymal sperm using a modified Norwegian extender and density gradient method could be effective to obtain functional spermatozoa for future assisted reproductive practices in endangered species.

Measurement and Prediction of the Flash Points for Flammable Liquid Mixtures with Non-flammable Component

  • Ha, Dong-Myeong;Yu, Hyun-Sik;Kang, Gyeun-Hee;Ann, Jeong-Jin;Lee, Sung-Jin
    • International Journal of Safety
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    • v.7 no.2
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    • pp.12-16
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    • 2008
  • Lower flash points for the binary systems, carbon tetrachloride+o-xylene and water+n-butanol were measured by Pensky-Martens closed cup tester. The Raoult's law and optimization method using van Laar equation were used to predict the lower flash points and were compared with experimental data. The calculated values based on the optimization method were found to be better than those based on the Raoult's law.

Safety and Immunogenicity of Salmonella enterica Serovar Typhimurium llaB in Mice

  • CHO SUN-A;LEE IN-SOO;PARK JONG-HWAN;SEOK SEUNG-HYEOK;LEE HUI-YOUNG;KIM DONG-JAE;BACK MIN-WON;LEE SEOK-HO;HUR SOOK-JIN;BAN SANG-JA;LEE YOO-KYOUNG;PARK JAE-HAK
    • Journal of Microbiology and Biotechnology
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    • v.15 no.3
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    • pp.609-615
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    • 2005
  • The safety and immunogenicity of an attenuated recombinant Salmonella vaccine strain, Salmonella enterica serovar Typhimurium llaB, was assessed. This vaccine strain could survive in low pH condition, and its ability of intracellular survival did not differ from that of S. enterica serovar Typhimurium UK1, which is the wild-type of the vaccine strain. The mortality of the mice orally administered with the vaccine strain was $50\%$ at the dose of $10^7$ CFU. All mice administered with $10^5\;or\;10^3$ CFU of the vaccine strain survived for 3 days postinoculation (pi). However, all mice administered with more than $10^3$ CFU of the vaccine strain died within 3 days pi. To examine the protective effect of the vaccine strain, mice were orally immunized with $10^4\;and\;10^6$ CFU of the bacteria. Control mice were given with 0.5 ml of phosphate buffered saline (PBS). After 8 days, the mice were challenged with $10^9$ CFU of S. enterica serovar Typhimurium UK1, and mortality was examined for 5 days. The survival rates of the mice immunized with $10^4\;and\;10^6$ CFU of the vaccine strain were $60\%\;and\;80\%$, respectively, whereas all control mice died within 2 days after challenging. To investigate the immunogenicity of S. enterica serovar Typhimurium llaB, mice were orally immunized with $10^5\;or\;10^6$ CFU ml of the vaccine strain. Five mice of each group were sacrificed at 5 and 12 days after immunization, and results showed that immunization of the vaccine strain led to increases of IgG1, IgG2, and IgM titers against S. enterica serovar Typhimurium UK1 in mouse sera, cytokine expressions such as IL-2, IL-4, IL-6, and IL-10 in spleen, and the lymphocyte proliferation response to mitogens (concanavalin A or LPS) stimulation.