• Title/Summary/Keyword: labeling data

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Comprehensive Analysis of Proteomic Differences between Escherichia coli K-12 and B Strains Using Multiplexed Isobaric Tandem Mass Tag (TMT) Labeling

  • Han, Mee-Jung
    • Journal of Microbiology and Biotechnology
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    • v.27 no.11
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    • pp.2028-2036
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    • 2017
  • The Escherichia coli K-12 and B strains are among the most frequently used bacterial hosts for scientific research and biotechnological applications. However, omics analyses have revealed that E. coli K-12 and B exhibit notably different genotypic and phenotypic attributes, even though they were derived from the same ancestor. In a previous study, we identified a limited number of proteins from the two strains using two-dimensional gel electrophoresis and tandem mass spectrometry (MS/MS). In this study, an in-depth analysis of the physiological behavior of the E. coli K-12 and B strains at the proteomic level was performed using six-plex isobaric tandem mass tag-based quantitative MS. Additionally, the best lysis buffer for increasing the efficiency of protein extraction was selected from three tested buffers prior to the quantitative proteomic analysis. This study identifies the largest number of proteins in the two E. coli strains reported to date and is the first to show the dynamics of these proteins. Notable differences in proteins associated with key cellular properties, including some metabolic pathways, the biosynthesis and degradation of amino acids, membrane integrity, cellular tolerance, and motility, were found between the two representative strains. Compared with previous studies, these proteomic results provide a more holistic view of the overall state of E. coli cells based on a single proteomic study and reveal significant insights into why the two strains show distinct phenotypes. Additionally, the resulting data provide in-depth information that will help fine-tune processes in the future.

NMR study of the interaction of T4 Endonuclease V with DNA

  • Lee, Bong-Jin;Im, Hoo-Kang;Hyungmi Lihm;Yu, Jun-Suk
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1995.04a
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    • pp.80-80
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    • 1995
  • T4 Endonuclease V (Mw 16,000) acts as a repair enzyme for UV induced pyrimidine dimers in DNA. Many researchers have studied the biochemical characteristics of the enzyme. However the precise action mechanism of T4 endo V has not fully elucidated yet. In our laboratory NMR spectroscopy technique is being used for the structural study of T4 endo V. Because of its low temperature stability and high content of ${\alpha}$-helix, the conventional $^1$H NMR technique was inapplicable. Therefore we utilized stable isotope labeling technique and so far prepared about 10 amino acid specific labeled proteins. The HSQC spectra of amino acid specific labeled proteins will help us to interpret the triple resonance 3D, 4D data which are under processing, We also studied the behaviors of specific amino acid residues whose roles might be critical. When the enzyme labeled by $\^$15/N-Thr was mixed with the substrate oligonucleotide (semispecific -TT- sequence), one crosspeak in its HSQC spectrum was completely desappeared, which means that one of seven Thr residues is in the binding site of the enzyme with DNA, This result is well consistent with previous report that implicated the Thr 2 residue in the activity of the enzyme. Similar studies were carried on the behaviors of Arg and Tyr residues.

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Protective Effects of Gamipalmi-hwan on Elastase-induced Apoptosis of A549 Cells (가미팔미환(加味八味丸)의 elastase 유도성 A549 세포사멸에 대한 보호효과)

  • Oh, Ji-Seok;Park, Yang-Chun
    • The Journal of Korean Medicine
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    • v.31 no.2
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    • pp.137-148
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    • 2010
  • Objective: This study aimed to evaluate the protective effects of Gamipalmi-hwan (GPH) on elastase-induced lung cell injury. Materials and Methods: As an in vitro model of emphysema, the current study was performed to investigate potential activity of GPH in regulating injury responses of A549 human type II cell line mediated by elastase treatment. Results: GPH treatment increased the number of A549 cells which was reduced by elastase digestion. Elastin protein level, which was reduced by elastase treatment, was increased by GPH treatment. Labeling intensity with caspase 3 protein in elastase-treated cells was reduced by GPH treatment. Both Erk1/2 and Cdc2 protein levels, which were decreased by elastase treatment, were increased to a level similar to that of the normal cells. mRNA levels encoding IL-$1{\beta}$ and TNF-$\alpha$ were increased by elastase and then down-regulated by GPH. Conclusion: The present data suggest that A549 cells are subjected to inflammatory damage by elastase and can be recovered by GPH treatment. Further studies examining the protective activity of GPH in elastase-treated lung tissue would be useful for therapeutic strategies of emphysema treatment.

A study on importance of MSDS education (MSDS 교육의 중요성에 관한 연구)

  • Choi, Sung-Jai
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.15 no.6
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    • pp.209-215
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    • 2015
  • Following the semiconductor industry's growing, various types of toxic gases and caustic chemicals, HF(Hydrofluoric acid), HCI (Hydochloric acid), $H_2O_2$ (Hydrogen peroxide), $H_2SO_4$ (Sulfuric acid), and Piranha, were using on the semiconductor manufacturing process. Therefore many gas leakage accidents that produce huge losses of lives were caused by the processes. This research deeply considers two basic solutions that the necessity of MSDS education on university for reducing damage of lives and protecting life from chemical leak accidents such as a HF accident in Gumi, Korea and the use of GHS, REACH and the comprehension of propriety about using MSDS for keeping safety from conflagrations by released poison chemical materials.

Phellodendri Cortex Herbal-acupuncture Solution Induced Apoptosis in Human Cervical Cancer Cells, SNU-17

  • Seo, Yong-Seok;Seo, Jung-Chul;Lim, Seong-Chul;Jung, Tae-Young;Han, Sang-Won
    • Journal of Acupuncture Research
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    • v.22 no.2
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    • pp.103-110
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    • 2005
  • Phellodendri Cortex (PC) has been used traditionally in Korea for damp heat leukorrhea with thick, yellow, discharge, foul-smelling diarrhea or dysentery. We investigated whether the Phellodendri Cortex Herbal-acupuncture Solution (PCHS) induced cell-death on SNU-17, human cervical cancer cell. 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was performed to find out the cytotoxicity of PCHS. The cell death was identified as apoptosis from the results of 4, 6-diamidineo-2-phenylindole (DAPI) staining, terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. The expression of proapototic gene, Bax, was increased and the expression of apoptotic gene, Caspase-3, was also increased. Considering the above results, PCHS could induce the apoptosis on SNU-17, human cervical cancer cell, via Bax-related Caspase-3 activation. And it might provide the experimental data for the clinical use of Phellodendri Cortex on cervical cancer.

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Awareness and Information Acquisition Behavior Regarding Food Hygiene and Safety of College Students in Yeungnam Region (영남지역 대학생의 식품 위생 및 안전성에 대한 인식도와 정보획득행동에 관한 연구)

  • Kim, Mee-Ra;Kim, Hyo-Chung
    • Journal of the East Asian Society of Dietary Life
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    • v.22 no.2
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    • pp.305-314
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    • 2012
  • This study examined awareness and information acquisition behavior regarding food hygiene and safety and information search behavior of college students. The sample was obtained from 284 students in Yeungnam region through a self-administered questionnaire. To describe characteristics of the respondents, frequency distributions were used. In addition, $t$ tests, analysis of variance and Duncan multiple range tests were conducted. Data were analyzed by SPSS Windows V.18.0. The results of the study were as follows: (1) most respondents were concerned about food hygiene and safety. They were mostly interested in the expiration date label on breads, milk/dairy products, fish products and meat products, and price of cookies. Many respondents answered that foods produced and distributed in Korea were not safe. The respondents were most worried about endocrine disruptors among various food risk factors. The respondents received information on food hygiene and safety from blogs, mini-homepages, or Kin-searches on the Internet, electronic media, and food labeling in decreasing order.

Characteristics of Purchasers and Non-Purchasers of Environmental Products (환경상품 구매자와 비구매자의 특성 비교 분석)

  • 안창희;정순희
    • Journal of Families and Better Life
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    • v.22 no.1
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    • pp.55-64
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    • 2004
  • The major purpose of this study was to investigate purchasing behavior of environmental products by Korean consumers, which will ultimately help foster sustainable consumption. Environmental consciousness, environmental behaviors, level of awareness of environmental products, and purchasing of environmental products were examined. Mean differences between purchasers and non-purchasers of environmental products were compared in terms of environmental consciousness and behaviors, and the level of awareness of environmental products. A survey was conducted on 310 consumers in the greater Seoul metropolitan area. The data were analyzed by frequencies, percentages, logistic regression, and t-tests using a variable for interval scale and a variable for nominal scale. There were significant mean differences between purchasers and non-purchasers of environmental products on three variables of environmental consciousness and behaviors. Those who were educated on environmental issues showed a higher preference in purchasing environmental products. Among socio-demographic variables, the income level was the only variable that showed a significant mean difference between the two groups. Also, there was a remarkable difference in purchasing behavior between the two groups. For the purchasers of environmental products, the purchasing decisions took into account environment-friendliness of products. Non-purchasers of environmental products put more emphasis on price or quality of products. The results of the logistic regression analysis indicated that those who had higher education, who viewed environmental pollution as a serious problem, and who are more cognizant of the environmental labeling tend to purchase more environmental products.

The Block Segmentation and Extraction of Layout Information In Document (문서의 영역분리와 레이아웃 정보의 추출)

  • 조용주;남궁재찬
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.17 no.10
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    • pp.1131-1146
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    • 1992
  • In this paper, we suggest a new algorithm applied to the segmentation of published documents to obtain constituent and layout information of document. Firstly, we begin the process of blocking and labeling on a 300dpi scanned document. Secondly, we classify the blocked document by individual sub-regions. Thirdly, we group sub-regions into graphic areas and text areas. Finally, we extract information for layout recognition by using the data. From an experiment on papers of an academic society, we obtain the above 98% of region classification rate and extraction rate of information for the layout recognition.

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Color Image Segmentations of a Vitiligo Skin Image with Android Platform Smartphone (안드로이드 기반의 스마트폰을 활용한 백반증 피부 영상 분할)

  • Park, Sang-Eun;Kim, Hyun-Tae;Kim, Jeong-Hwan;Kim, Kyeong-Seop
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.63 no.1
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    • pp.173-178
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    • 2014
  • In this study, the new color image processing algorithms with an android-based mobile device are developed to detect the abnormal color densities in a skin image and interpret them as the vitiligo lesions. Our proposed method is firstly based on transforming RGB data into HSI domain and segmenting the imag into the vitiligo-skin candidates by applying Otsu's threshold algorithm. The structure elements for morphological image processing are suggested to delete the spurious regions in vitiligo regions and the image blob labeling algorithm is applied to compare RGB color densities of the abnormal skin region with them of a region of interest. Our suggested color image processing algorithms are implemented with an android-platform smartphone and thus a mobile device can be utilized to diagnose or monitor the patient's skin conditions under the environments of pervasive healthcare services.

Evaluation of the Serving Sizes of Packaged Processed Food in Korea

  • Chang, Soon-Ok
    • Nutritional Sciences
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    • v.5 no.1
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    • pp.41-46
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    • 2002
  • This study was carried out to evaluate the adequacy of serving sizes of those packaged processed foods in Korea which are designed to provids one meal or snack for one person. There is a lack of data on reference serving sizes which are essential components in the nutrition information provided on food labels. After compiling the average weight of packaged processed foods available in the market in Korea, a questionnaire was formulated to evaluate the adequacy of the serving sizes on 56 packaged processed food items of 188products using 25 female university students as a consumer group. The results showed that 65.5% of the packaged processed foods had adequate serving sizes. In general, current packaged sizes for bread and carbonated drinks were found to be too large. The proposed reference serving sizes in this research appear to be somewhat different from the ones suggested by the Korean Nutrition Society s $7^{th}$ Recommended Dietary Allowances for Koreans and by the Food and Drug Administration (USFDA) in the U.S. The serving sizes suggested by the USFDA appear to be too large for beverages and too small for snack foods when these are applied to the Korean population. It is suggested that the size of beverages in the Korean market should be reduced, and smaller sub-packets of snacks (each for one serving) should be packaged in a larger pack, for ease of use of nutrition information by consumers as well as for the reduction of food waste. In the future, other representative population groups should be included in the determination of reference serving sizes.