• Title/Summary/Keyword: labeling data

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Dynamics of the mobile insert helix in the domain III-IV of Aux/IAA17 probed by site-directed spin labeling and paramagnetic NMR spectroscopy

  • Han, Mookyoung;Suh, Jeong-Yong
    • Journal of the Korean Magnetic Resonance Society
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    • v.19 no.2
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    • pp.61-66
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    • 2015
  • The plant hormone auxin is involved in all stages of plant development. Aux/IAAs are the transcriptional repressors that bind to the Auxin Response Factors (ARFs) to regulate the gene expression upon auxin release. Aux/IAA have highly conserved C-terminal domains (domains III-IV) that mediate both homotypic and heterotypic interactions between Aux/IAA and ARF family proteins. Recent studies revealed that the conserved domains III-IV share a common ${\beta}$-grasp fold that oligomerizes in a front-to-back manner. In particular, Aux/IAA contains a mobile insert helix in the domain III-IV, whereas ARFs do not. Here, we investigated the dynamics of the insert helix using paramagnetic NMR spectroscopy. The insert helix exhibited fast motions in the ps-ns time scale from $^{15}N$ relaxation data, but the amplitude of the motion is likely limited to the local neighborhood. Our result suggests that the motion of the helix may have functional implications in protein-protein interactions for transcriptional regulations.

A Review on Mutagenicity Testing for Hazard Classification of Chemicals at Work: Focusing on in vivo Micronucleus Test for Allyl Chloride

  • Rim, Kyung-Taek;Kim, Soo-Jin
    • Safety and Health at Work
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    • v.6 no.3
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    • pp.184-191
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    • 2015
  • Chemical mutagenicity is a major hazard that is important to workers' health. Despite the use of large amounts of allyl chloride, the available mutagenicity data for this chemical remains controversial. To clarify the mutagenicity of allyl chloride and because a micronucleus (MN) test had not yet been conducted, we screened for MN induction by using male ICR mice bone marrow cells. The test results indicated that this chemical is not mutagenic under the test conditions. In this paper, the regulatory test battery and several assay combinations used to determine the genotoxic potential of chemicals in the workplace have been described. Further application of these assays may prove useful in future development strategies of hazard evaluations of industrial chemicals. This study also should help to improve the testing of this chemical by commonly used mutagenicity testing methods and investigations on the underlying mechanisms and could be applicable for workers' health.

On the 2D Vision Inspection Algorithm for Semiconductor Chip Package (반도체 패키지의 2차원 비전 검사 알고리즘에 관한 연구)

  • Yu, Sang-Hyun;Kim, Yong-Kwan
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.12C
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    • pp.1157-1164
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    • 2006
  • In this paper, we proposed a method for measuring accurate positions and sizes of package and balls in a micro BGA. To find defects of BGA accurately, we focused on finding positions of package and balls. After labeling, we detected connected components of package and balls using feature parameters. After the detection of package component, we measured position and size of package by employing rectangular model which was constructed by the package information. After the detection of the ball components, we measured positions and diameters of balls by employing circular models which were constructed by the ball informations. We did calibration based on landmarks to measure real length, and we compared the measured results with the SEM data. Finally, we found that the accuracy of the proposed method is 94% in terms of ball's radius.

Analysis of the Effects of an Educational Program regarding Food Safety for Children (아동의 식품 안전 교육 프로그램에 대한 효과 분석)

  • Kim, Mee-Ra;Jeon, Mi-Kyung;Kim, Hyo-Chung
    • Journal of the Korean Home Economics Association
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    • v.44 no.6 s.220
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    • pp.113-120
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    • 2006
  • The purpose of this study was to examine the effects of an educational program regarding food safety for children. The data were collected from the results of a self-administered questionnaire that was given to 510 6th grade elementary school students in the Youngnam region who had undergone a food safety education program. The results of this study were as follows. First, the educational need for food safety was high in each food safety category, with the educational need for prevention against foodborne illness being the highest of the 10 categories. On the basis of the study results, educational materials were developed which targeted personal hygiene, food labeling, food purchase, food washing, food storage, cooking, foodborne illness prevention, food additives, endocrine disruptors, and illegal foods. Additionally, about 37% of the respondents aswered that they preferred the food safety education to be conducted at home. Second, the food safety education significantly improved the levels of knowledge and behavior of children toward food safety. These results imply that food safety education that addresses the concerns of children and their parents' is needed to improve the level of knowledge and behavior toward food safety.

Development of Real-Time Displacement Measurement System for Multiple Moving Objects of construction structures using Image Processing Techniques (영상처리기술을 이용한 건축 구조물의 실시간 변위측정 시스템의 개발)

  • Kim, Sung-Wook;Seo, Jin-Ho;Kim, Sang-Bong
    • Proceedings of the KSME Conference
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    • 2003.04a
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    • pp.764-769
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    • 2003
  • The paper introduces a development result for displacement measurement system of multiple moving objects based on image processing technique. The image processing method adopts inertia moment theory for obtaining the centroid of the targets and basic processing algorithms of gray, binary, closing, labeling and etc. To get precise displacement measurement in spite of multiple moving targets, a CCD camera with zoom is used and the position of camera is changed by a pan/tilt system. The fiducial marks on the fixed positions are used as the sensing points for the image processing to recognize the position errors in directions of X -Y coordinates. The precise alignment device is pan /tilt of X - Y type and the pan/tilt is controlled by DC servomotors which are driven by 80c196kc microprocessor based controller. The centers of the fiducial marks are obtained by a inertia moment method. By applying the developed precise position control system for multiple targets, the displacement of multiple moving targets are detected automatically and are stored in the database system in a real time. By using database system and internet, displacement data can be confirmed at a great distance and analyzed. The developed system shows the effectiveness such that it realizes the precision about 0.12mm in the position control of X -Y coordinates.

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A Syllabic Segmentation Method for the Korean Continuous Speech (우리말 연속음성의 음절 분할법)

  • 한학용;고시영;허강인
    • The Journal of the Acoustical Society of Korea
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    • v.20 no.3
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    • pp.70-75
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    • 2001
  • This paper proposes a syllabic segmentation method for the korean continuous speech. This method are formed three major steps as follows. (1) labeling the vowel, consonants, silence units and forming the Token the sequence of speech data using the segmental parameter in the time domain, pitch, energy, ZCR and PVR. (2) scanning the Token in the structure of korean syllable using the parser designed by the finite state automata, and (3) re-segmenting the syllable parts witch have two or more syllables using the pseudo-syllable nucleus information. Experimental results for the capability evaluation toward the proposed method regarding to the continuous words and sentence units are 73.5%, 85.9%, respectively.

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SOPPY : A sentiment detection tool for personal online retailing

  • Sidek, Nurliyana Jaafar;Song, Mi-Hwa
    • International Journal of Internet, Broadcasting and Communication
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    • v.9 no.3
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    • pp.59-69
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    • 2017
  • The best 'hub' to communicate with the citizen is using social media to marketing the business. However, there has several issued and the most common issue that face in critical is a capital issue. This issue is always highlight because most of automatic sentiment detection tool for Facebook or any other social media price is expensive and they lack of technical skills in order to control the tool. Therefore, in directly they have some obstacle to get faster product's feedback from customers. Thus, the personal online retailing need to struggle to stay in market because they need to compete with successful online company such as G-market. Sentiment analysis also known as opinion mining. Aim of this research is develop the tool that allow user to automatic detect the sentiment comment on social media account. RAD model methodology is chosen since its have several phases could produce more activities and output. Soppy tool will be develop using Microsoft Visual. In order to generate an accurate sentiment detection, the functionality testing will be use to find the effectiveness of this Soppy tool. This proposed automated Soppy Tool would be able to provide a platform to measure the impact of the customer sentiment over the postings on their social media site. The results and findings from the impact measurement could then be use as a recommendation in the developing or reviewing to enhance the capability and the profit to their personal online retailing company.

Measurement of maximum deviation of leads using partial image of SMD mounted on PCB (PCB에 장착된 SMD 의 부분영상을 이용한 리드의 최대 벗어난 양의 측정)

  • Shin, Dong-Won;You, Jun-Ho
    • Journal of Institute of Control, Robotics and Systems
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    • v.5 no.6
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    • pp.698-704
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    • 1999
  • There are several types of defects of SMDs mounted on PCB, that is, missing components, misalignment, wrong parts and poor solder joints. This research study mainly focuses on measuring of deviation of SMD leads using the partial image of component, not using the full image. This processing based on the partial image has the advantage of the reduction in calculation time compared to the full image. Since position of lead is calculated with respect of the reduction in calculation time compared to the full image. Since position of lead is calculated with respect to pad, the accuracy of the system is not dependent on percise positioning stage. The grabbed image of gray scale is converted into binary format using a cutomatic threshold. After small fragments in the image is removed by a series of morphology operations such as opening and closing, the centroids of PCB pads and SMD leads is obtained together with labeling of blobs. Translational shift and rotationial angle of SMD are succedingly estimated using above information and chip data. The expression that can calculate the maximum deviation of leads with respect to PCB pads has been derived, and inferior mounting of SMD is judged by a given criterion. Some experiments have been executed to verify this measuring scheme.

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Target segmentation in non-homogeneous infrared images using a PCA plane and an adaptive Gaussian kernel

  • Kim, Yong Min;Park, Ki Tae;Moon, Young Shik
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.9 no.6
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    • pp.2302-2316
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    • 2015
  • We propose an efficient method of extracting targets within a region of interest in non-homogeneous infrared images by using a principal component analysis (PCA) plane and adaptive Gaussian kernel. Existing approaches for extracting targets have been limited to using only the intensity values of the pixels in a target region. However, it is difficult to extract the target regions effectively because the intensity values of the target region are mixed with the background intensity values. To overcome this problem, we propose a novel PCA based approach consisting of three steps. In the first step, we apply a PCA technique minimizing the total least-square errors of an IR image. In the second step, we generate a binary image that consists of pixels with higher values than the plane, and then calculate the second derivative of the sum of the square errors (SDSSE). In the final step, an iteration is performed until the convergence criteria is met, including the SDSSE, angle and labeling value. Therefore, a Gaussian kernel is weighted in addition to the PCA plane with the non-removed data from the previous step. Experimental results show that the proposed method achieves better segmentation performance than the existing method.

Measurement of job Satisfaction of Nurses and Health Workers in Health Centers (직무만족 측정 도구개발)

  • 박성애;윤순녕
    • Journal of Korean Academy of Nursing
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    • v.22 no.3
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    • pp.316-324
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    • 1992
  • The purpose of this study was to develop a ratio scale to measure job satisfaction of nurses and health workers in health centers. Of the 224 health centers in Korea, 105 were drawn as the sample using disproportion리 stratified random sampling. The data collection period was from May 1 to June 30, 1990 and 771 nurses and health workers working at 75 health centers participated. Subjects were instructed to rate, according to the level of satisfaction, from one to four points, 42 items revised from the tool made by Stamps et. al. (1978). The internal consistency reliability of the tool was measured by Cronbach $\alpha$ and the corrected item-total correlation coefficient. As a result of the item analysis, one item was excluded since the item had negative correlation with total items. As a result of factor analysis on the 41 items with varimax or above. Labeling and numbering of 6 factors were as follows ; factor 1 (8 items) ; Professional status of job. factor 2 (6 items) ; Interaction. factor 3 (8 items) ; Autonomy. factor 4 (8 items) ; Administration. factor 5 (7 items) ; Pay. factor 6 (4 items) ; Job(Task). Further studies are needed to identify the relationship between this tool and the effectiveness of the health center and more research is needed for generalization.

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