• Asian-Australasian Journal of Animal Sciences
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• v.33 no.6
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• pp.879-887
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• 2020

Objective: Numerous studies have indicated that the apoptosis and proliferation of granulosa cells (GCs) are closely related to the normal growth and development of follicles and ovaries. Previous evidence has suggested that miR-126-3p might get involved in the apoptosis and proliferation of GCs, and phosphatidylinositol 3-kinase regulatory subunit 2 (PIK3R2) gene has been predicted as one target of miR-126-3p. However, the molecular regulation of miR-126-3p on PIK3R2 and the effects of PIK3R2 on porcine GCs apoptosis and proliferation remain virtually unexplored. Methods: In this study, using porcine GCs as a cellular model, luciferase report assay, mutation and deletion were applied to verify the targeting relationship between miR-126-3p and PIK3R2. Annexin-V/PI staining and 5-ethynyl-2'-deoxyuridine assay were applied to explore the effect of PIK3R2 on GCs apoptosis and proliferation, respectively. Real-time quantitative polymerase chain reaction and Western Blot were applied to explore the regulation of miR-126-3p on PIK3R2 expression. Results: We found that miR-126-3p targeted at PIK3R2 and inhibited its mRNA and protein expression. Knockdown of PIK3R2 significantly inhibited the apoptosis and promoted the proliferation of porcine GCs, and significantly down-regulated the mRNA expression of several key genes of PI3K pathway such as insulin-like growth factor 1 receptor (IGF1R), insulin receptor (INSR), pyruvate dehydrogenase kinase 1 (PDK1), and serine/threonine kinase 1 (AKT1). Conclusion: MiR-126-3p might target and inhibit the mRNA and protein expressions of PIK3R2, thereby inhibiting GC apoptosis and promoting GC proliferation by down-regulating several key genes of the PI3K pathway, IGF1R, INSR, PDK1, and AKT1. These findings would provide great insight into further exploring the molecular regulation of miR-126-3p and PIK3R2 on the functions of GCs during the folliculogenesis in female mammals.

• 한국유가공학회:학술대회논문집
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• 2004.06a
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• pp.21-39
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• 2004

Fermented milks have been recognized as healthy foods since ancient times, but those using intestinal bacteria such as Bifidobacterium and lactic acid bacteria (LAB) are even more valuable from the standpoint of view of maintaining health. They have also now come to be recognized as important in the field of preventive medicine. Although advances in the medical sciences in the last 50 years have significantly increased the human lift span, an unfortunate fact is that many of us are now living long enough to experience chronic disorders such as coronary heart disease, hypertension, osteoporosis, diabetes and cancer. In recent years there has been renewed interest in health promotion and disease prevention by incorporating probiotic bacteria into foods to counteract harmful bacteria in the intestinal tract. Therefore, there are now a wide variety of commercial products containing prospective probiotics that claim health-promoting effects, such reductions in large botvel carcinogens and mutagens, antitumor properties, cholesterol-lowering effects, increased lactose digestion, relief from con-stipation, stimulation of immunocomponent cells and enhancement of phagocytosis. Two well-known representative probiotic is LAB and Bifidobaclerium. Traditional probiotic dairy strains of LAB which have been designated as GRAS (Generally Recognized As Safe) bacteria have a long history of safe use and most strains are considered comestible microorganisms with no pathogenic potential. Accordingly, there is considerable interest in extending the range of foods containing probiotic organisms from dairy foods to infant formulas, baby foods, and pharmaceuticals. In addition, the ingestion of probiotics, prebiotics, and symbiotic as wll as combinations of pro- and prebiotics has recently aroused renewed interest as enhancing the beneficial relationship between the host and intestinal microflora in both healthy and diseased indivisuals. Non-communicable chronic diseases such as cancer, cerebral hemorrhage, is chemic heart disease, and diabetes mellitus has recently been recognized as adult diseases in Japan as well as other countries. and are considered to be inevitably associated with aging. These diseases occur as a result of individual life styles. The Japanes Government. Ministry of Health, Labor and Welfare has proposed substituting the term 'adult diseases' with 'lifestyle-related diseases'. It has emphasized the importance of prevention rather than treatment. since the well-known increase in the elderly population in Japan is predicted to result in a variety of socioeconomic problems. n this lecture on the Present status of fermented milk products in Japan, I will report a strategy for the development of fermented milk products in Japan from the standpoint of view of research in Japan on LAB and Bifidobacteria. They could play an important role in preserving human health by controlling intestinal microflora capable of producing toxic effects on the host.

• Membrane Journal
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• v.16 no.1
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• pp.16-24
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• 2006

We report on the preparation and characterization of sulfonated polymer membranes containing perfluorocyclobutane (PFCB) units and fluorene units. The polymers were prepared through three synthetic steps, that is, the synthesis of a trifluorovinylether-terminated monomer, its thermal polymerization, and post-sulfonation using chlorosulfonic acid. A series of sulfonated polymers with different ion exchange capacity (IEC) were prepared by changing the content of chlorosulfonic acid during the post-sulfonation reaction. All the synthesized compounds were characterized by FT-IR, $^{1}H-NMR,\;^{19}F-NMR$, and Mass spectroscopy. As the content of chlorosulfonic acid increased, the SD, IEC, water uptake, and ion conductivity of the sulfonated polymer membranes increased. The sulfonated polymer 4 showed higher values of ion conductivity than the Nafion-$115^{\circledR}$ in a wide range of temperatures ($25{\sim}80^{\circ}C$).

• Journal of Korean Tunnelling and Underground Space Association
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• v.25 no.2
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• pp.175-186
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• 2023

The buffer materials in disposal hole are exposed to the decay heat from spent nuclear fuels and groundwater inflow through adjacent rockmass. Since understanding of thermal-hydro-mechanical-chemical (T-H-M-C) interaction in buffer material is crucial for predicting their long-term performance and safety of disposal repository, it is necessary to investigate the heating-hydration characteristics and consequent T-H-M-C behavior of the buffer materials under disposal conditions considering geochemical factors. In response, the Korea Atomic Energy Research Institute developed a laboratory-scale 'Lab.THMC' experiment system, which characterizes the T-H-M behavior of buffer materials under different geochemical conditions by analyzing heating-hydration process and stress changes. This technical report introduces the detail design of the Lab.THMC system, summarizes preliminary experimental results, and outlines future research plans.

• The Journal of the Korean dental association
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• v.51 no.5
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• pp.255-264
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• 2013

Many concept of occlusion have been introduced in the past, but there isn't any generally accepted unified theory since a single occlusion theory cannot represent the natural perfection, especially considering the difference in clinical experience, the dynamic occlusion, and individual diversity in mastication pattern. NAT(Naturgemasse Aufwachstechnik)/NFR((Natur Funktionsgerechte Rekonstruktion) occlusion is not just a theory on occlusion, but a clinical occlusion that can be practically applied to clinical practice by providing dynamic occlusion adjusted to the individual mastication pattern and biomechanics. This report aims to provide a guide to a clinically biomechanical occlusion through the teamwork of both the dentist and the lab technician by introducing the NAT occlusion through following: reviewing the concept of occlusion in terms of morphology, its correlation with NAT/NFR occlusion, reviewing the limitation of conventional occlusion theories, and introducing the clinical application of NAT/NFR.

• 한국생태학회:학술대회논문집
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• 1999.05a
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• pp.55-62
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• 1999

Four species of Paraphysomonas collected from the fast- ice covered area Syowa Station, East Ongul Island ($69^{\circ}00'S,\;39^{\circ}35'$) ,Antarctica occurred in the seawater throughout the year and occasionally in the sea ice. P.. antarctica is distributed to a water depth of 35m at 51.3 during the period from August 1983 to January 1984 and also down to 600m St. 5 in September 1983 at cell concentrations of 300-350 cells/ml. The Paraphysomonas spp. were dominant during the period from July to November 1983 in the area studied. The mode of the occurrence and vertical distribution of Paraphysomonas apparently coresponds to those of the bacteria and orgarnic debris-like matter in the seawater. The main components of the plankton population in the area studied, under ice-covered conditions, are Paraphysomonas, Choanoflagellates and bacteria. This work clarified that Paraphysomonas is one o f the most important bacterivores in the microbial loop of the Antarctic marine ecosystem.

• Proceedings of the Materials Research Society of Korea Conference
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• 2011.10a
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• pp.19.1-19.1
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• 2011

We report on the metal-insulator transition that occurs as a function of film thickness in ultrathin $La_{0.75}Sr_{0.25}VO_3$ films. The metal-insulator transition displays a critical thickness of 5 unit cell. Above the critical thickness, metallic films exhibit a temperature driven metal-insulator transition with weak localization behavior. With decreasing film thickness, oxygen octahedron rotation in the films increases, causing enhanced electron-electron correlation. The electron-electron correlations in ultrathin films induce the transition from metal to insulator in addition to Anderson localization.

• The Journal of Korean Medicine
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• v.27 no.4
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• pp.209-214
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• 2006

Objective : Report on a hepatitis B patient with abnormal AST/ALT, HBeAg(+), and habitual diarrhea stemming from irritable colon. Methods : Lab-evaluation of intervals of herb medicine treatment for a patient with hepatitis B with HBeAg and irritable colon. Result & Conclusion : AST/ALT level was normalized and did not rise again. Seroconversion to HBeAb repeatedly appeared just druing the time of the treatment, but typically repeated seroconversion between treatment period and no treatment period confirmed that the treatment was effective for the immune system against hepatitis B. The exact mechanism is not clear but the result provides an indication that oriental herbal medicine has potential capacity to treat liver diseases.

• The Journal of Korean Medicine
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• v.28 no.3 s.71
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• pp.108-115
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• 2007

Objectives : The purpose of this study was to investigate the effect of Sojukjeongwon-san (이하 SJJWS) on a rectal cancer patient. Methods : This clinical case was analyzed on a patient who was admitted on August 11th and discharged on September 7th, 2006. We recordedhis abdominal pain, urine, anal pain, bleeding, mucous stools, frequency and hardness and amount of the stools, weight, lab follow-ups and so on. Results : After the treatment, the chief complaints of the patient, such as rectal pain, bloody excrement, loss of weight, etc., had improved. Conclusion : In this study we suggest that SJJWS reduced symptoms for a rectal cancer patient and improved the patient's quality of life.

• Journal of information and communication convergence engineering
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• v.8 no.3
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• pp.281-288
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• 2010

This paper investigates the performance of IEEE 802.11p wireless access in vehicular environments (WAVE) system in small-scale fading models reported by Georgia Institute of Technology (Georgia Tech). We redesign the small-scale fading models to be applied to the computer simulation and develop the IEEE 802.11p WAVE physical layer simulator to provide the bit error rate and packet error rate performances. Moreover, a new channel estimator using decision directed channel estimation and linear minimum mean square error smoothing is proposed in order to improve the performance of the conventional least square channel estimator using two identical long training symbols. The simulation results are satisfactorily coincident with the scenarios of Georgia Tech report, and the proposed channel estimator significantly outperforms the conventional channel estimator.