• 한국어병학회지
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• 제26권3호
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• pp.303-310
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• 2013

2013년 3월부터 국립수산과학원 내수면양식연구센터에서는 종 보존 연구를 위해 1,000미의 드렁허리(Monopterus albus)를 양성하고 있었다. 그러던 중에 100미의 드렁허리에서 체색흑화, 점액과다, 항문돌출과 같은 임상증상을 보였으며 몇몇 개체들은 폐사하였다. 해부 검사한 결과 100미 전부 구두충에 감염된 것을 확인할 수 있었다. 이에 드렁허리에 감염된 구두충을 병리조직학적으로 관찰하였다. 구두충은 소화관 전반에서 관찰되었으며 개체 당 평균 19미의 충체를 확인할 수 있었다. 중감염된 개체에서는 구두충에 의한 장폐색 증상을 보였으며 충체는 소화관 점막하층에 proboscis를 침투시켜 기생하고 있었다. 특징적으로 위와 장에서 점막상피내 충혈과 점막하조직내 호산성 세포의 침윤, 염증반응, 기생충성 육아종 소견을 보였다. 일부 개체에서는 위선상피의 공포화와 장 점막층의 괴사도 관찰되었다. 소화관 외 기타장기에서는 특징적인 병변은 관찰되지 않았다. 본 연구로 드렁허리에 감염된 구두충의 감염정도를 알 수 있었고, 향후 드렁허리 양식에 큰 도움이 될 것으로 보인다.

• 한국동물학회지
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• 제33권1호
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• pp.103-107
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• 1990

BSA로 면역시킨 드렁허리의 혈청에서 lgM 유사면역글로불린의 정제한 후 그 구조적 특성을 구명하였다. 면역후 항혈청에서는 항원특이 항체능이 증가되었다. 정제된 lgM 유사면역글로불린은 199kD의 분자량을 갖는 단량체 4개가 결합된 800kD의 단백질이었으며, 단량체는 70kD인 H-사슬 2개와 29.5kD인 L-사슬 2개가 결합된 형태였다. 또한 드렁허리의 lgM 유사면역글로불린은 lgM의 특성과 같이 포유류 적혈구에 대하여 약한 응집력을 나타냈다.

• Parasites, Hosts and Diseases
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• 제58권6호
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• pp.695-699
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• 2020

Present study was performed to know the infection status of Gnathostoma sp. larvae in swamp eels from Cambodia. We purchased total 30 Asian swamp eels, Monopterus albus, from local markets in Pursat and Takeo Provinces and Phnom Penh on May and November 2017 and May 2018. All collected eels were transferred to our laboratory with ice and each of them was examined by artificial digestion method. A total of 15 larval gnathostomes (1-5 larvae) were detected from 55.6% (5/9) swamp eels in Pursat Province. No larval gnathostomes were found in 21 swamp eels in Takeo Province and Phnom Penh. The advanced third-stage larvae (AdL3) detected were 2.575-3.825 (3.250) mm in length and 0.375-0.425 (0.386) mm in width. They had the characteristic head bulb (av. 0.104×0.218 mm) with 4 rows of hooklets, long muscular esophagus (1.048 mm), and 2 pairs of cervical sacs (0.615 mm). The number of hooklets in 4 rows on the head bulb was 41, 44, 47, and 50. In scanning electron microscopy, characteristic features were 4 rows of hooklets on the head bulb, cervical papillae, tegumental spines regularly arranged in transverse striations, and anus. The larval gnathostomes were identified as AdL3 of Gnathostoma spinigerum based on the morphological characters. By the present study, it has been confirmed that G. spinigerum larvae are infected in Asian swamp eels, M. albus, in Pursat Province, Cambodia.

• Parasites, Hosts and Diseases
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• 제53권5호
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• pp.619-625
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• 2015

The present study was performed to determine the infection status of swamp eels with Gnathostoma sp. larvae in Myanmar. We purchased total 37 Asian swamp eels, Monopterus albus, from a local market in Yangon in June and December 2013 and 2014. All collected eels were transferred with ice to our laboratory and each of them was examined by the artificial digestion technique. A total of 401 larval gnathostomes (1-96 larvae/eel) were detected in 33 (89.2%) swamp eels. Most of the larvae (n=383; 95.5%) were found in the muscle. The remaining 18 larvae were detected in the viscera. The advanced third-stage larvae ($AdL_3$) were 2.3-4.4 mm long and 0.25-0.425 mm wide. The characteristic head bulb ($0.093{\times}0.221mm$ in average size) with 4 rows of hooklets, muscular long esophagus (1.025 mm), and 2 pairs of cervical sacs (0.574 mm) were observed by light microscopy. The average number of hooklets in the 1st, 2nd, 3rd, and 4th rows was 41, 45, 48, and 51, respectively. As scanning electron microscopic findings, the characteristic 4-5 rows of hooklets on the head bulb, a cervical papilla, tegumental spines regularly arranged in the transverse striations, and an anus were well observed. Based on these morphological characters, they were identified as the AdL3 of Gnathostoma spinigerum. By the present study, it has been confirmed for the first time that Asian swamp eels, M. albus, from Yangon, Myanmar are heavily infected with G. spinigerum larvae.