• Journal of fish pathology
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• v.26 no.3
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• pp.303-310
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• 2013

Since March in 2013, Inland Aquaculture Research Center, NFRDI has cultivated 1,000 wild swamp eel(Monopterus albus) for species conservation research. While cultivating, 100 fishes showed clinical sign that darkness color, mucus hypersecretion and anus rubor. Even some of them were died. Result of anatomical test, all 100 fishes were infected with intestinal parasite, acanthocephalan. So we were going to determine the case of acanthocephalans infection in swamp eel(Monopterus albus) as histopathologically. Acanthocephalan was founded in alimentary canal only. Parasite were confirmed 19 unit in individual fish, averagely. Heavy infected fishes were confirmed enterocleisis by acanthocephalans. Worms were attachment in submucosa layer of alimentary canal by invading proboscis. Characteristic symptoms were observed in stomach and intestine, including hyperemia in mucous epithelium, infiltration of eosinophills in submucosa layer, inflammation, parasitic granuloma. Some fishes showed vacuolization of gastricgland epithelium, necrosis of intestinal mucosa. Other organs, excluding alimentary canal, were not found lesion. The results of this study, the effect of the acanthocephalan infection on swamp eel(Monopterus albus) could find and it seems to be a big help in the future swamp eel(Monopterus albus) cultivation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.2
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• pp.163-169
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• 2018

This study examined the expression of heat shock protein 70 (Hsp70) in red seabream Pagrus major infected by the, acanthocephalan parasites Longicollum pagrosomi. We cloned the full-length Hsp70 cDNA from the liver of the red seabream. The full-length cDNA had a 1,950 bp open reading frame (ORF) that encoded a protein of 650 amino acids. The deduced amino acid sequence of Hsp70 contained all of the conserved Hsp70 family signature sequences and an adenosine triphosphate (ATP)/guanosine triphosphate (GTP) binding motif, including the EEVD (consensus sequence that terminates in Hsp70 family) consensus sequence. The expression of Hsp70 mRNA was upregulated int the fish head-kidney and liver, as determined by quantitative real-time PCR. We quantified the Hsp70 mRNA expression in normal red seabream and fish infected fish by L. pagrosomi. The expression of Hsp70 mRNA was significantly higher in the infected red seabream. These results suggest that Hsp70 play a role of protection against stress and inflammation caused by the parasite and may help maintain homeostasis.

• Korean Journal of Veterinary Service
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• v.40 no.3
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• pp.169-175
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• 2017

Acanthocephalan worms were harvested from the posterior intestines of the cultured marine fishes from January to July 2016 in a fish market located in Daejeon metropolitan city, Republic of Korea. Totally 450 cultured fishes (rock fish 100, olive flounder 250, red sea bream 100) were surveyed. Of the 100 red sea bream fish, 37 fishes (37%) were diagnosed as positive for Longicollum pagrosomi by light, electron microscopic and molecular examination. The number of worm was 25~78 (mean $51{\pm}13$). However, we can't found any worm from the cultured rock fish and olive flounder. After sequencing, none of Pomphorhynchidae family are not identical based on 18S rRNA gene, and this data were identified with the first report for 18S rRNA gene sequence of L. pagrosomi. Furthermore, we confirmed that L. pagrosomi of the cultured red sea bream in Republic of Korea is very common parasite.