• Journal of Veterinary Science
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• 제22권6호
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• pp.41.1-41.14
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• 2021

Background: Our previously prepared ceftiofur (CEF) hydrochloride oily suspension shows potential wide applications for controlling swine Streptococcus suis infections, while the irrational dose has not been formulated. Objectives: The rational dose regimens of CEF oily suspension against S. suis were systematically studied using a pharmacokinetic-pharmacodynamic model method. Methods: The healthy and infected pigs were intramuscularly administered CEF hydrochloride oily suspension at a single dose of 5 mg/kg, and then the plasma and pulmonary epithelial lining fluid (PELF) were collected at different times. The minimum inhibitory concentration (MIC), minimal bactericidal concentration, mutant prevention concentration (MPC), post-antibiotic effect (PAE), and time-killing curves were determined. Subsequently, the area under the curve by the MIC (AUC_0-24h/MIC) values of desfuroylceftiofur (DFC) in the PELF was obtained by integrating in vivo pharmacokinetic data of the infected pigs and ex vivo pharmacodynamic data using the sigmoid E_max (Hill) equation. The dose was calculated based on the AUC_0-24h/MIC values for bacteriostatic action, bactericidal action, and bacterial elimination. Results: The peak concentration, the area under the concentration-time curve, and the time to peak for PELF's DFC were 24.76 ± 0.92 ㎍/mL, 811.99 ± 54.70 ㎍·h/mL, and 8.00 h in healthy pigs, and 33.04 ± 0.99 ㎍/mL, 735.85 ± 26.20 ㎍·h/mL, and 8.00 h in infected pigs, respectively. The MIC of PELF's DFC against S. suis strain was 0.25 ㎍/mL. There was strong concentration-dependent activity as determined by MPC, PAE, and the time-killing curves. The AUC_0-24h/MIC values of PELF's DFC for bacteriostatic activity, bactericidal activity, and virtual eradication of bacteria were 6.54 h, 9.69 h, and 11.49 h, respectively. Thus, a dosage regimen of 1.94 mg/kg every 72 h could be sufficient to reach bactericidal activity. Conclusions: A rational dosage regimen was recommended, and it could assist in increasing the treatment effectiveness of CEF hydrochloride oily suspension against S. Suis infections.

• 보존과학연구
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• 통권22호
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• pp.5-25
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• 2001

The germicidal and insecticidal properties of volatile components extracted from star anise(Illicium verum Hooker filius) and clove (Eugenia caryophyllata THUNBERG)were evaluated against five microorganisms and three insects for the purpose of developing biocidal active substances from medicinal plants. The volatile components of star anise and clove showed strong antimicrobial effect against Aspergillus niger, Penicillium funiculosum, Mucor hiemalis, Trichoderma viride, and Aureobasidium pullulans. The extracts of each medicine also showed insecticidal effects against Sitophilusoryzae L., Lyctus linearis GOZE, and Reticulitermes spertus kyushuensis Morimoto. Fumigant toxicities to adult insects were determined. In the case of fumiganttoxicity, the extract of star anise showed 100% mortality against R. spertus, S.oryzae, and L. linearis at rates of $2.5\mu\ell$, $50\mu\ell$, $250\mu\ell$/filter paper, respectively but showed no killing effects by clove. The volatile components of star anise and clove were investigated by means of GC/MS. The main constitute, anethole among 20components from star anise and eugenol among 9 components from clove were identified. The mixture of star anise and clove as the volume ratio of 2 : 1 showed higher properties for antimicrobial and insecticidal effect than each volatile component. A. niger was inhibited by the mixture(125ml/$m^3$) for up to 10 days of exposure. Also, from the result of observing state change of organic materials by volatile extracts of star anise and clove, volatile extracts effects have no effect on natural organic materials of organic cultural properties and can be used as biological control agent. As research contents as above, the insecticidal and germicidal agents from star anise and clove and the mixture of them were more efficient and high level to prevent biological damage for conservation of organic cultural properties. So they may be used in new development of biologicalinsecticidal and germicidal agents for conservation of cultural properties.

• Asian-Australasian Journal of Animal Sciences
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• 제25권7호
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• pp.988-993
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• 2012

Four experimental diets were formulated to contain 700 g/kg cereal with decreasing levels of wheat:barley inclusion. Diet 1 contained 700 g/kg wheat, diet 2 contained 600 g/kg wheat and 100 g/kg barley, diet 3 contained 500 g/kg wheat and 200 g/kg barley and diet 4 contained 400 g/kg wheat and 300 g/kg barley. The diets were offered to pigs on three trials to investigate effects on the performance of individually (n = 72) and group housed (n = 480) pigs and on nutrient digestibility in pigs housed in metabolizm crates (n = 24). Performance was assessed from 10 wks of age until slaughter and carcass characteristics were measured. For the group performance study, one pig from each pen (in total 24) at 10, 15 wks and at finish were slaughtered to ascertain scores for stomach ulceration, stomach weights and intestinal length. Level of wheat inclusion did not significantly (p>0.05) affect liveweight gain (LWG) or feed conversion ratio (FCR). Feed intake was lowest (p<0.05) for individually housed pigs offered diets containing 700 g/kg wheat during the 10-15 wk period, which indicated that individually housed pigs attempted to eat to a constant energy intake. There was little evidence of stomach ulceration across treatments and increasing wheat inclusion had no detrimental effect. Higher levels of wheat inclusion tended to increase backfat depth at the $P_2$ position which could lead to increasing grading penalties in a commercial situation although more research is required in this area. Increasing level of wheat inclusion increased digestible energy (DE) content but the lack of effect on FCR and killing out percentage indicated that utilization of energy from barley and wheat was similar. Digestibility coefficients increased linearly with increasing wheat content, which can be attributed to the lower level of fibre and higher level of starch in wheat compared with barley.

• Applied Microscopy
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• 제38권3호
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• pp.245-250
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• 2008

전립선암은 남성 사망의 주된 원인이 되는 치명적인 질병으로 남성호르몬 의존형과 비의존형이 있다. Etoposide (Eto)는 현재 전립선암을 치료하는 데 사용하고 있으나 남성호르몬 비의존형에는 치료 성공률이 낮아서, 보다 효과적인 치료제 개발이 절실히 요구되어왔다. 본 연구는 항산화제인 vitamin C (VC)가 전립선 암세포에 어떠한 역할을 하는지 알아보고자 남성호르몬 의존형-전립선 암세포인 LNCaP와 비의존형 암세포인 DU-145에 비교적 낮은 농도의 Eto와 VC를 복합처리한 결과, Eto만을 투여한 것과 비교하여 암세포의 성장이 현저하게 억제되었고, apoptosis의 발생률 역시 유의적으로 증가하였다(p<0.05). 이러한 결과는 VC가 전립선암 치료제로 사용하고 있는 Eto의 효과를 증가시킬 수 있음을 강력히 시사하는 것이다.

• 약학회지
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• 제40권3호
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• pp.357-368
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• 1996

The in vitro antibacterial activity of a novel fluoroquinolone, DWP20373(1-Cyclopropyl-6-fluoro-8-methoxy-7-(2,7-diazabicyclo[3,3,0]oct-4-ene-7-yl)-1,4-dihydro-4 oxoquino line-3carboxylic acid) was compared with those of ciprofloxacin (CPFX), sparfloxacin (SPFX) and ofloxacin (OFLX). DWP20373 was more active than SPFX and OFLX but was less potent than CPFX against gram-negative bacteria. DWP20373 showed an excellent activity against L-MRSA and H-MRSA ($MlC_{90}=0.781{\sim}1.563{\mu}g/ml$).The activity of DWP20373 decreased moderately in the presence of 5mM $Mg^{2+}$. However, pH and serum had no effect on the activity of DWP20373. DWP20373 possessed a rapid bactericidal activity against gram-positive and gram-negative strains.

• 한국초지조사료학회지
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• 제4권3호
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• pp.226-234
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• 1984

Italian ryegrass의 종자생산(種子生産)을 위한 파종적기(播種適期)와 파종적량(播種適量)을 구명(究明)하기 위하여 4배체품종인 Tetrone을 공시(供試)하여 $'83.\;8.\;20.{\sim}9.\;25.$까지 10일간격(日間隔)으로 5개(個) 파종기(播種期)와 $1{\sim}4kg/10a$의 4개(個) 수준(水準)의 파종량(播種量)으로 분할구(分割區) 배치(配置) 3 반복(反覆) 시험(試驗)한바 그 결과(結果)를 요약(要約)하면 아래와 같다. 1. 월동전(越冬前)에 형성(形成)된 약자(藥子)를 동사면(凍死面)에서 볼때 완전동사(完全凍死), 기부(基部)는 생존(生存)하고 생장점(生長点) 동사(凍死),그리고 생장점(生長点)도 동사(凍死)하지 않은 것 3군(群)으로 구분(區分)할 수 있었다. 2. 9월(月) 9 일이전(日以前) 파종(播種)은 월동전(越冬前) 생육량(生育量)이 과다(果多)하여 추위에 약(弱)하였고 분약시원체가 엽초내에 생존(生存)해 있어도 모체의 고엽량(枯葉量)이 많아 봄에 출현(出現)이 늦고 따라서 출수(出穗)가 늦으며 간장(稈長)도 짧고 종자수량(種子收量)도 저조(低調)하였다. 3. 생육(生育)이 중용(中庸)인 것 중에서 생장점(生長点)은 동해(凍害)를 받았으나 하부(下部)가 생존(生存)해 있고 동해(凍害)를 받지 않은 것에서는 하부절(下部節)의 분약시원체가 발육(發育), 일찍 출현(出現)하여 이것들의 대부분(大部分)이 유효경이 되었다. 4. 간장(稈長)과 수장(穗長) 그리고 이삭의 숙기(熟期)는 파종기(播種期), 파종량(播種量)의 영향(影響)보다도 약자출현기(藥子出現期)의 영향(影響)이 더컸다. 5. 종자수량(種子收量) 구성요인(構成要因)중 단위면적당(單位面積當) 이삭수가 수량(收量)에 제일 크게 영향(影響)을 미쳤다. 6. 월동(越冬)을 잘시켜 종자(種子) 생산년도(生産年度)에 단위면적당(單位面積當) 충분(充分)한 이삭수를 확보(確保)하여 수량(收量)을 높이기 위해서는 수원지방(水原地方)에 파종적기(播種適期)는 9월(月) 하순(下旬)이었고 파종량(播種量)은 10a당(當) 2-3 kg였다. 특(特)히 파종적기내(播種適期內)에서 일찍 파종(播種)할 때는 2kg/10a 늦게 파종(播種)할 때는 3kg/10a가 좋았다.

• Food Science and Biotechnology
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• 제14권4호
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• pp.479-486
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• 2005

Arabinoxylan, a complex polysaccharide in cereal cell walls, has recently received research attention as a biological response modifier. The immunomodulating effect of arabinoxylans from rice bran (AXrb) was studied using a combined process of extrusion and commercial hemicellulase treatment in order to elucidate the augmentation mechanism of cell-mediated immunity in vitro. The cytotoxicity of mouse spleen lymphocytes against YAC-1 tumor cells was significantly enhanced by treatment with AXrb at $10-100\;{\mu}g/mL$. In an attempt to investigate the mechanism by which AXrb enhance NK cytotoxicity, we examined the effect of AXrb on cytokine production by spleen lymphocytes. Culture supernatants of the cells incubated with AXrb were collected and analyzed for IL-2 and IFN-${\gamma}$ synthesis by ELISA. IL-2 and IFN-${\gamma}$ production were increased significantly. These results suggest that AXrb may induce Th1 immune responses. Macrophages play an important role in host defenses against tumors by killing them and producing secretory products, which protect against bacterial, viral infection and malignant cell growth. AXrb were examined for their ability to induce secretory and cellular responses in murine peritoneal macrophages. When macrophages were treated with various concentrations ($10-100\;{\mu}g/mL$) of AXrb, AXrb induced tumoricidal activity, as well as increasing phagocytosis and the production of NO, $H_2O_2$, TNF-${\alpha}$, IL-$1{\beta}$, and IL-6. These results indicate that reactive oxygen species, reactive nitrogen species, and inflammatory cytokines are likely to be the major mediators of tumoricidal activity in AXrb-treated macrophages. Therefore, AXrb may be useful in cancer immunotherapy and it is anticipated that AXrb obtained using extrusion and subsequent enzyme treatment can be used as an ingredient in nutraceuticals and cereal-based functional food.

• 한국콘텐츠학회논문지
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• 제20권4호
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• pp.189-201
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• 2020

동영상으로 자신의 일상을 기록해 친구들과 소통하는 것이 Z세대나 밀레니얼 세대에게 트렌드로 자리 잡으면서 브이로그에 대한 관심이 높아지고 있다. 본 연구는 유튜브 브이로그 이용 경험이 있는 20-30대 일반인 168명을 대상으로 브이로그 이용 동기와 이용자 특성이 브이로그 이용 만족과 지속이용의도에 미치는 영향을 분석하였다. 연구결과, 본 연구에서 설정한 모든 가설이 채택되었다. 즉, 브이로그 이용 동기(습관 및 시간보내기, 정보추구, 오락 및 휴식, 관음주의)와 이용자 특성(개방성, 감정적 동일시)이 이용 만족에 정적인 영향을 미치는 것으로 나타났으며, 브이로그에 대한 이용 만족 역시 지속이용의도에 유의미한 영향을 미치는 것으로 나타났다. 본 연구는 새로운 콘텐츠 분야라고 할 수 있는 브이로그에 대한 실증적 접근을 통해 여러 이론적, 실제적 시사점을 제공했다는 점에 의의가 있다.

• The Korean Journal of Physiology and Pharmacology
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• 제1권2호
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• pp.209-219
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• 1997

Previous studies have demonstrated that oxidative stress involving generation of reactive oxygen species (ROS) is responsible for the cytotoxic action of $TNF{\alpha}$. Protective effect of small heat shock proteins (small HSP) against diverse oxidative stress conditions has been suggeted. Although overexpression of small hsp was shown to provide an enhanced survival of $TNF{\alpha}$-sensitive cells when challenged with $TNF{\alpha}$, neither the nature of $TNF{\alpha}$-induced cytotoxicity nor the protective mechanism of small HSP has not been completely understood. In this study, we have attempted to determine whether $TNF{\alpha}$ induces oxidative DNA damage in $TNF{\alpha}$-sensitive L929 cells. We chose to measure the level of 8-hydroxy-2'-deoxyguanosine (8 ohdG), which has been increasingly recognized as one of the most sensitive markers of oxidative DNA damage. Our results clearly demonstrated that the level of 8 ohdG increased in L929 cells in a $TNF{\alpha}$ dose-dependent manner. Subsequently, we asked whether small HSP has a protective effect on $TNF{\alpha}$-induced oxidative DNA damage. To accomplish this goal, we have stably transfected L929 cells with mouse small hsp cDNA (hsp25) since these cells are devoid of endogenous small hsps. We found that $TNF{\alpha}$-induced 8 ohdG was decreased in cells overexpressing exogenous small hsp. We also found that the cell killing activity of $TNF{\alpha}$ was decreased in these cells as measured by clonogenic survival. Taken together, results from the current study show that cytotoxic mechanism of $TNF{\alpha}$ involves oxidative damage of DNA and that overexpression of the small hsp reduces this oxidative damage. We suggest that the reduction of oxidative DNA damage is one of the most important protective mechanisms of small HSP against $TNF{\alpha}$.

• IMMUNE NETWORK
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• 제5권4호
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• pp.247-251
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• 2005

Background: Natural killer (NK) cells are CD3 (-) CD14 (-) CD56 (+) lymphocytes. They play an important role in the body's innate immune response. They can induce spontaneous killing of cancer cells or virus-infected cells via the Fas/Fas ligand or the granzyme/perforin systems. The corticotropin-releasing hormone (CRH) is an important regulator for the body's stress response. It promotes proliferation and migration of various cancer cells through the CRH type 1 receptor under stress, and also inhibits NK or T cell activity. However, the relationship of CRH and NK cell migration to the target has not been confirmed. Herein, we study the effect of CRH on NK cell migration. Methods: We used the human NK cell line, NK-92MI, and tested the expression of CRH receptor type 1 on NK-92MI by RT-PCR. This was to examine the effect of CRH on tumor and NK cell migration, thus NK cells (NK-92MI) were incubated with or without CRH and then each CRH treated cell's migration ability compared to that of the CRH untreated group. Results: We confirmed that CRH receptor type 1 is expressed in NK-92MI. CRH can decrease NK cell migration in a time-/dose-dependent manner. Conclusion: These data suggest CRH can inhibit NK cell migration to target cells.