• Journal of Life Science
• /
• v.16 no.3 s.76
• /
• pp.522-526
• /
• 2006

To investigate the effects of UV-B on fatty acid composition, lipid peroxidation and biochemical defense responses of plant, kidney bean (Phaseolus vulgaris L.) was subjected to enhanced UV-B irradiation [daily dose : 0.02.(No UV-B) and 11.36 (enhanced UV-B) $kJ\;m^{-2};UV-B_{BE}$] for 3 weeks. UV-B drastically inhibited both height and dry weight of kidney bean. The content of malondialdehyde significantly increased by about 50% after 3 weeks of UV-B irradiation. The ratio of unsaturated to saturated fatty acids of kidney bean was increased by UV-B irradiation. Three major polyamines of kidney bean leaves : putrescine, spermidine and spermine, were observed. All of the polyamine contents were increased with UV-B irradiation. These results suggested that enhanced UV-B radiation caused oxidative stress on lipids and biochemical protection responses might be activated to prevent from damaging effects of oxidative stress generated by UV-B irradiation.

• Proceedings of the Botanical Society of Korea Conference
• /
• 2002.04a
• /
• pp.89-89
• /
• 2002

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.33 no.3
• /
• pp.518-522
• /
• 2004

In this study we examined effects of a new dietary supplement on the lipid level and body weight. The efficacy of this weight-reduction supplement, based on natural ingredients consisting of Phaseolus vulgaris, Garcinia cambogia, and microstalline cellulose, was investigated by randomized, placebo-controlled double-blind study. The 36 subjects were assigned evenly into weight-reduction supplemented (Active) and placebo groups (Placebo). The supplement could reduce the absorption of different types of sugar from the gastro-intestinal tract. A significant difference in weight reduction was shown in the active group (3.5 kg). Body composition measurements indicated that about 34% of fat loss in the active group could be achieved. These findings suggest that this dietary supplement could reduce body weight and fat gains, and its inhibitory effects might lead to obesity improvement.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.83-83
• /
• 2017

The National Agrobiodiversity Center (NAS, RDA, Republic of Korea) has continually collected new valuable genetic resources. In this study, we regenerated conserved kidney bean (Phaseolus vulgaris L.) germplasm which couldn't be available because of seed quantity and quality, and we also surveyed their morphological characters for the sustainable utilization. A total of 431 kidney bean accessions were regenerated and 18 morphological traits were surveyed according to the characterization guideline of RDA Genebank. Among the surveyed traits, flowering time ranged from May 23 to September 4 and 73.8% of tested accessions were mainly flowering in June. The maturity time ranged from July 1 to October 15 and main flowering time was July (91.4%). For plant type, 270 accs (62.6%) were climbing type followed by medium type of 86 accs (20.0%) and dwarf type of 65 accs (15.1%). The seed coat colors were various; yellow (34.6%), white (22.3%), brown (17.9%), red (10.7%), black (5.8%), violet (11%), pink (1.4%), navy (0.9%). Principal component analysis indicated that five principal components (PCs) with Eigen values >1 accounted for more than 65.8% variability. The first PC was more related to growth habits such as growth type, flowering time, and plant type. The second and third PCs showed higher values of the pigment characters such as seed coat color, flower color, and pod color. In fourth and fifty PCs, there were the higher positive values of the pod shapes. Our results provided insight into the characteristics kidney beans, thus the utilization basis of kidney beans might be elevated for bio-industry.

• Korean Journal of Food Science and Technology
• /
• v.33 no.1
• /
• pp.117-121
• /
• 2001

White kidney bean, Phaseolus vulgaris, contains proteinaceous inhibitors of ${\alpha}-amylase$. Two inhibitors have been purified by conventional protein fractionation methods such as ethanol precipitation, ammonium sulfate fractionation, DEAE-Sephadex ion exchange chromatography and Sephadex G-100 gel chromatography. The inhibitors were purified as I-1 and I-2 based on their elution order from the DEAE-Sephadex column. The overall purification ratio were about 15.0 and 14.8 for I-1 and I-2, respectively. The molecular weights of purified ${\alpha}-amylase$ inhibitors were 50,000 and 45,000 determined by Sephadex G-100 gel filtration and SDS-polyacrylamide gel electrophoresis. They contain $17.6{\sim}17%$ of carbohydrate, $70{\sim}80%$ of protein. The carbohydrates were composed of glucose : xylose : mannose : N-acetylglucosamine (5 : 3 : 50 : 42).

• Korean Journal of Food Science and Technology
• /
• v.19 no.6
• /
• pp.528-532
• /
• 1987

Lipids of kidney bean were extracted by the mixture of chloroform-methanol-water (1:2:0.8 v/v), fractionated into neutral lipids, glycolipids and phospholipids by silicic acid column chromatography, and the composition of these lipid classes were determined by TLC and GLC. The lipid content of kidney bean was 1.9%, and the lipid was consisted of 48.3% neutral lipids. 7.5% glycolipids and 44.2% phospholipids. Triglyceride was the major component of neutral lipids (64.6%). The major glycolipid and phosphlolipid were esterified steryl glycoside (38.3%) and phosphatidyl choline (32.9%). The major fatty acids of kidney bean lipid were linolenic, linoleic, palmitic and oleic acid. Linolenic acid contents were very high to be 37.1% in total lipid and 50.5% in neutral lipid.

• Korean Journal of Food Science and Technology
• /
• v.19 no.6
• /
• pp.533-536
• /
• 1987

Lipids of Kidney bean were extracted by the mixture of chloroform-methanol-water (1:2:0.8, v/v) and from these lipids, triglycerides were separated by thin-layer chromatography(TLC) and then reseparated into different fractions by high performance liquid chromatography (HPLC) depending on the polarity of the triglycerides. The triglyceride and fatty acid composition of these fractions were determined by gas liquid chromatography (GLC). From these results, the major triglycerides of Kidney bean lipids were estimated to be $C_{18:2}C_{18:3}C_{18:3}$ (26.6%), $C_{18:2}C_{18:2}C_{18:3}$ (18.5%), $C_{18:3}C_{18:3}C_{18:3}$ (14.9%).

• Korean Journal of Soil Science and Fertilizer
• /
• v.25 no.2
• /
• pp.149-154
• /
• 1992

A study was carried out to know the effects of two kinds of paper mill sludge manure compost(SMC) on the growth and chemical components of kidney bean(Phaseolus vulgaris) : Control(SMC-0) contained chemical fertilizer only, sludge manure compost-1(SMC-1) contained 18% of swine manure and 8% of sawdust of the total weight of the manure in addition to the chemical fertilizer, and sludge manure compost-2(SMC-2) contained swine manure without sawdust a third of the total by weight on a fresh weight basis, 1) Kidney bean treated with SMC-1 and SMC-2 showed the increasing tendency of values meristem height, stem diameter, pod number per plot and total grain yield when compared to control(SMC-0) treatment 2) Though there was not a significant difference between SMC-1 and SMC-2 treatments on the growth, SMC-1 tended to increase the value of meristem height, stem diameter, pod number per plot and total grain yield of kidney bean, 3) The contents of aluminium, iron and zinc in kidney bean were higher than other elements, though there was no significant difference between the treatments, And those of chromium, lead and cadmium were negligible. 4) From the results obtained, it can be said that SMC application has more favorable effects on the growth of the plant than control(SMC-0). The effects of SMC-1 application on the growth of kidney bean was more favorable than those of SMC-2.

• Korean Journal of Plant Tissue Culture
• /
• v.25 no.6
• /
• pp.477-485
• /
• 1998

To underatand in vitro regulation of differentiation, the effects of growth regulators and nitrogen source on metabolism of cell wall polysaccharides in suspension culture of kidney bean (Phaseolus vulgaris L.) were investigated. The suspension cells (cell clusters) were directly induced from the epicotyl segments of the seedlings, which were cultivated in MS medium supplemented with 1.0mg/L of 2,4-D and 0.5 mg/L of kinetin. When compared with cell wall sugar contents of the epicotyl segments, the cellulose content of the suspension-cultured cells decreased; while the pectin and hemicellulose content increased; suggesting increases of rhamnogalacturonan I and arabinogalactan IIduring the dedifferentiation, respectively, The effects of growth regulators(2,4-D, 1.0mg/L and kinetin, 0.5mg/L) and nitrogen source (potasium nitrate, 19.0mg/L and ammonium nitrate, 16.5 g/L) in the medium on the proliferation and the turnover of the cell wall polysaccharides were investigated for 30 days. In the medium with growth regulators and without nitrogen source, the proliferation rate was extremely high (16 folds). Growth regulators and nitrogen source increased the pectin content. Analysis of neutral sugar composition of pectin fraction showed that nitrogen source enhanced rhamnose level remarkably, suggesting that rhamnogalacturonan I was the one most likely synthesized. In hemicellulose fraction, growth regulators reduced arabinose level, suggesting that arabinogalactan II was degraded. And nitrogen source reduced galactose level, suggesting that xyloglucan was also degraded.

• Journal of Bio-Environment Control
• /
• v.17 no.2
• /
• pp.96-100
• /
• 2008

To extend the shelf-life of kidney bean (Phaseolus vulgaris L.) after harvest, we investigated the effect of packaging material and storage temperature. In case of film package, there was no weight loss during storage, but in paper-box package, remarkable weight loss occurred as storage period extended and storage temperature risen. Firmness of kidney bean was greater in low temperature than ambient temperature storage. Content of vitamin C was decreased rapidly during storage, and in paper package, low temperature storage group had lower decrease of vitamin C than that of room temperature. Total chlorophyll content was not difference among storage temperatures in film package treatments. However, in paper-box package, decrease of chlorophyll in room temperature storage was greater than that of low temperature ($8{\sim}10^{\circ}C$). In terms of freshness of kidney bean, we suggest that shelf-life by low temperature storage ($8{\sim}10^{\circ}C$) after PP film or paper package was 8 days, but that by room temperature storage after paper package was 4 days.