• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.2
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• pp.174-180
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• 2016

In this study, the effects of fermented goat milk (F-GM) on immunological activity and physical strength were examined. Splenocytes obtained from mice administered with F-GM showed increased responsinveness to mitogens, concanavalin-A (ConA), and lipopolysaccharide (LPS). Treatment with F-GM also significantly augmented production of interleukin (IL)-2 and interferon (IFN)-${\gamma}$, but not IL-4 or IL-10 from ConA-stimulated splenocytes. The activity of F-GM administration to enhance production of IL-2 and IFN-${\gamma}$ was confirmed based on mRNA expression of these cytokines by reverse transcription polymerase chain reaction. After immunization with keyhole limpet hemocyanin (KLH, 20 mg/mouse), mice administered F-GM showed significantly higher antibody titers against KLH than those of phosphate-buffered saline-treated mice, and showed the highest titer 5 weeks after KLH immunization. Analysis for determining isotypes of antibodies revealed that administration of F-GM elicited KLH-specific antibody titers of IgG1, IgG2a, and IgM. In a delayed type hypersensitivity (DTH) test carried out 7 weeks after the primary immunization, F-GM-treated mice showed a higher DTH reaction than the control mice. Furthermore, the swimming test found that administration of F-GM significantly increased swimming time. These results suggest that administration of F-GM enhances not only immune responses against antigens but also physical strength.

• Journal of Korean Orthopaedic Sports Medicine
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• v.8 no.1
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• pp.19-25
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• 2009

Purpose: We report mid to long - term results of meniscal transplantation and evaluate the important factors for successful outcomes. Materials and Methods: Between December 1999 and September 2002, 25 meniscal transplantations were performed using fresh frozen allograft. The lateral meniscus was transplanted in 19 cases and medial meniscus in 6 cases. The mean age was 34 years (range, 17~50 years) and the mean follow up was 54.8 months (range, 6~85 months). Preoperative measurements were made using a ruler graded in millimeters. Lateral meniscus was fixed by keyhole technique and medial meniscus was fixed by double bone plug technique with suturing the periphery of the meniscal transplant. All patients were evaluated with Knee Assessment Scoring System (KASS), Lysholm knee score, and Tegner activity scale for daily activity. Results: Symptoms improved in all cases. The average KASS score increased from 61.7 preoperatively to 83.8 postoperatively. The average Lysholm knee score increased from 77.7 preoperatively to 87.7 postoperatively (excellent in 3 cases, good in 17 cases, fair 4 cases, poor 1 case). But painful swellings were 3 cases, numbness in 1 case, and granuloma due to non-absorbable suture material in 1 case. Peroneal nerve palsy in 1 case was recovered after 6 weeks postoperatively. Conclusion: Meniscal allograft transplantation after subtotal or total menisectomy can significantly relieve pain and improve function of the knee joint. The exact preoperative sizing and secure fixation are essential for successful outcomes.

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.4
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• pp.275-278
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• 2006

Google Earth enables people to easily find information linked to geographical locations. Google Earth consists of a collection of zoomable satellite images laid over a 3-D Earth model and any geographically referenced information can be uploaded to the Web and then downloaded directly into Google Earth. This can be achieved by encoding in Google's open file format, KML (Keyhole Markup Language), where it is visible as a new layer superimposed on the satellite images. We used KML to create and share fine resolution gridded temperature data projected to 3 climatological normal years between 2011-2100 to visualize the site-specific warming and the resultant earlier blooming of spring flowers over the Korean Peninsula. Gridded temperature and phonology data were initially prepared in ArcGIS GRID format and converted to image files (.png), which can be loaded as new layers on Google Earth. We used a high resolution LCD monitor with a 2,560 by 1,600 resolution driven by a dual link DVI card to facilitate visual effects during the demonstration.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.29 no.3
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• pp.134-149
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• 2016

Objectives : Gal-Geun-Tang (GT) has been described from SANGHAN in Korean traditional medicine and known to act against cold, fever, hypertension, and nasal catarrh. However, little has yet been learned about the effect of GT on immune function. In the current study, in vitro and in vivo immunomodulatory activity of GT (water extract) was investigated.Methods : Water extract of GT induced in vitro proliferation of spleen cells and significantly increased their proliferative responses during anti-CD3 activation. Using purified splenic T and B cells, it was revealed that GT has a mitogenic activity to B cells and promotes their proliferation induced by lipopolysaccharide, whereas T cell proliferation was not triggered and GT was rather inhibitory to T cell activation caused by anti-CD3 antibody. In the presence of antigen presenting cells (APC), GT addition resulted in a significant increase of IFNγ and IL-4, but not IL-2, production. However, addition of high concentration (1,000㎍/㎖) of GT led to a marked reduction in T cell cytokine production and under such condition, GT facilitated apoptosis of T cells when examined by flow cytometry with propidium iodide staining.Results : In vivo immunomdulation of GT was also investigated using a mouse model. Following keyhole limpet hemocyanin (KLH) immunization, GT (1 ㎎/day) was orally administered for 9 days. Cell numbers in thymus, spleen and peripheral blood were not altered by GT administration, indicating that such dose is not immunotoxic. Cell numbers in draining lymph nodes (LN) and ex vivo Ag-specific proliferation of LN cells were significantly elevated by GT administration. However, any preferential stimulation of T or B and CD4⁺ or CD8⁺ T cell subpopulations was not observed in a flow cytometric analysis of LN cells. This result shows that GT does not promote in vivo B cell proliferation while GT enhances Ag-specific proliferation of LN cells, unlike what was observed in vitro.Conclusions : For a further understanding of in vivo immunomodulatory activity of GT, ex vivo cytokine production of LN cells obtained from KLH-immunized mice was evaluated. Ag-specific IFNγ production was significantly higher in GT-treated mice when compared to PBS-treated control mice. In contrast, IL-4 production in GT-treated group was comparable to control group unlike to in vitro data. In addition, GT administration did not result in any significant differences in serum levels of Ig (IgM, IgG1 and IgG2a) between GT-treated and control groups. Taken together, these data strongly support that GT promotes immune response, more profoundly type 1 helper T cell (Th1) activity and GT may be applicable for treatment of intracellular parasite infection such as viral diseases.

• Journal of Navigation and Port Research
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• v.34 no.1
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• pp.9-14
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• 2010

Nautical Publications is a special purpose book, or a specially compiled database, that is issued officially by or on the authority of a Government, authorized Hydrographic Office and is designed to meet the requirements of marine navigation such as SOLAS and PSC. Nautical publications include List of Lights, Sailing directions. The SNPWG(Standardization Nautical Publications Working Group) from the IHO is working to implement in computer systems all those elements of interest for Hydrography and Navigation Concerning nautical publications' digitization process, the SNPWG has defined three types of nautical publications: Type 1 : Hard copy(NP1), Type 2 : Digital version, A slight modification of type 1(NP2), Type 3 : Digital version for ECDIS(NP3). Nautical Publications is essential with nautical charts as reference information to navigate. Development of digital nautical publications is needed for liaison with the chart production system, up-to-dateness of hydrographic information, improvement of service. But, Korean status is positioned in NP1. In our study, we surveyed the present status of nautical publications for development strategy of digital nautical publications. We build database of south coast of sailing direction, develop a manager program, convert the contents to KML( Keyhole Markup Language), develop a user program.

• Journal of Welding and Joining
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• v.18 no.2
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• pp.213-213
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• 2000

This study was performed to investigate types and formation mechanism of cracks in two Al alloy welds, A5083 and A7NO1 spot-welded by pulse Nd: YAG laser, using SEM, EPMA and Micro-XRD. In the weld zone, three types of crack were observed: center line crack($C_{C}$), diagonal crack($C_{D}$), and U shape crack($C_{U}$). Also, HAZ crack($C_{H}$), was observed in the HAZ region, furthermore, mixing crack($C_{M}$), consisting of diagonal crack and HAZ crack was observed.White film was formed at the hot crack region in the fractured surface after it was immersed to 10%NaOH water. In the case of A5083 alloy, white films in C crack and $C_D crack region were composed of low melting phases, Fe₂Si$Al_8$ and eutectic phases, Mg₂Al₃ and Mg₂Si. Such films observed near HAZ crack were also consist of eutectic Mg₂Al₃. In the case of A7N01 alloy, eutectic phases of CuAl₂, $Mg_{32}$ (Al,Zn) ₃, MgZn₂, Al₂CuMg and Mg₂Si were observed in the whitely etched films near $C_{C}$ crack and $C_{D}$ crack regions. The formation of liquid films was due to the segregation of Mg, Si, Fe in the case of A5083 alloy and Zn, Mg, Cu, Si in the case of A7N01 aooly, respectively.The $C_{D}$ and $C_{C}$ cracks were regarded as a result of the occurrence of tensile strain during the welding process. The formation of $C_{M}$ crack is likely to be due to the presence of liquid film at the grain boundary near the fusion line in the base metal as well as in the weld fusion zone during solidification. The $C_{U}$ crack is considered a result of the collapsed keyhole through incomplete closure during rapid solidification. (Received October 7, 1999)

• Biomolecules & Therapeutics
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• v.3 no.4
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• pp.266-272
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• 1995

The analysis of membrane receptors for hormones and neurotransmitters has progressed considerably by pharmacological and biochemical means and more recently through the use of specific antibodies. To generate and characterize a moloclonal antibody against $\beta$-adrenergic receptor, a synthetic $\beta$2-adrenergic receptor peptide (Phe-Gly-Asn-Phe-Trp-Cys-Phe-Trp-Thr-Ser-lle-Asp-Val-Leu) which may comprise part of $\beta$-adrenergic receptor ligand binding pocket was coupled to Keyhole Limpet Hemocyanin (KLH) and used as an immunogen. Male BALB/C mice were immunized with this antigen and the immunized spleen was fused with myeloma SP2/0-Ag14 cells to produce monoclonal antibodies. Two clones were obtained but one of monoclonal antibodies, mAb5G09, was used throughout in this study because the other clone, mAb5All showed weak immunoreactivity against KLH as well. The mouse monoclonal antibody mAb5G09 produced in this study showed immunoreactivity to peptide-KLH conjugates and also to human A43l cells and guinea pig lung $\beta$2-adrenergic receptor as revealed by ELISA and western blot. In the course of determination of the effects of mAb5G09 on $\beta$-receptor ligand binding, it was observed that mAb5G09 specifically bound $\beta$-adrenergic radioligand [$^3$H]dihydroalprenolol (DHA) with a dissociation constant (Kd) of 60 nM. The [$^3$H]DHA binding activity of mAb5G09 had characteristics of immunoglobulins and the binding activity was not observed in the control anti-KLH monoclonal antibody. The monoclonal antibody, mAb5G09 produced in this study may provide useful models for the study of the structure of receptor binding sites.

• Korean Journal of Materials Research
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• v.28 no.11
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• pp.663-670
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• 2018

H13 tool steels are widely used as metallic mold materials due to their high hardness and thermal stability. Recently, many studies are undertaken to satisfy the demands for manufacturing the complex shape of the mold using a 3D printing technique. It is reported that the mechanical properties of 3D printed materials are lower than those of commercial forged alloys owing to micropores. In this study, we investigate the effect of microstructures and defects on mechanical properties in the 3D printed H13 tool steels. H13 tool steel is fabricated using a selective laser melting(SLM) process with a scan speed of 200 mm/s and a layer thickness of $25{\mu}m$. Microstructures are observed and porosities are measured by optical and scanning electron microscopy in the X-, Y-, and Z-directions with various the build heights. Tiny keyhole type pores are observed with a porosity of 0.4 %, which shows the lowest porosity in the center region. The measured Vickers hardness is around 550 HV and the yield and tensile strength are 1400 and 1700 MPa, respectively. The tensile properties are predicted using two empirical equations through the measured values of the Vickers hardness. The prediction of tensile strength has high accuracy with the experimental data of the 3D printed H13 tool steel. The effects of porosities and unmelted powders on mechanical properties are also elucidated by the metallic fractography analysis to understand tensile and fracture behavior.

• Korean Journal of Heritage: History & Science
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• v.52 no.2
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• pp.268-291
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• 2019

In this paper, the features and meanings of the Yocoanabo (cave tombs) excavated in the Gongju and Buyeo regions were examined. The creation of the Yocoanabo in the Korean Peninsula was related to the situation in which the powers of the Northern Kyushu (九州) and Yeongsangang (榮山江) River Basin played certain roles and maintained power in the interactions of Wa and Baekje in the late 5th century until the early 6th century. The people buried in the Yocoanabo in the Gongju and Buyeo regions are most closely related to the Buzen (豊前) area in northern Kyushu. They also interacted with the Yeongsangang River Basin communities. The Yocoanabo in Gongju and Buyeo confirms the settlement of the Wa people. There were collective settlements such as the Danjiri (丹芝里) site on the outskirts of Woongjin (熊津). However, most of them were mixed with small local groups located on the main roads connecting Woongjin and Sabi (泗?) through the Geum River and land routes. Unlike the keyhole-shaped tombs of the Youngsangang River Basin, the Yocoanabo was not built until the 7th century. This explains differences in the backgrounds of the two types of graves. The people buried in the Yocoanabo are considered to have settled in the Baekje Kingdom and to have been responsible for practical work in the political and economic negotiations between Baekje and Wa. They came to the Baekje Kingdom collectively in connection with certain historical events. However, it had been presumed that most of the people came individually and dispersed to the local communities of Gonju and Buyeo.

• Korean Journal of Heritage: History & Science
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• v.43 no.3
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• pp.208-241
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• 2010

This study was conducted to examine the chronology of Taesil Seokham(Taesil Stone Boxes) stored in Taesil of the Joseon Palace and classify the styles to discuss how these boxes have developed. By doing so, this study defined the archaeological styles of taesil seokhams in the chronological order. Although taesil seokhams are placed when taesil are first created, they do not have any texts engraved on them and it is difficult to gain information only from examining taesil seokham. However, Taejiseok(memorial stones buried in Taesil) and Agibi(gravestones buried in Taesil) were created along with taesil. These resources were examined and compared with literature records to find when each taesil seokhams was created. The critical elements to consider for the chronological arrangement were both the cover and container of the boxes and the transition of their style could be classified into four major stages. In detail, the Joseon Dynasty's taesil seokhams initially inherited the style of the Goryeo Dynasty to be formed into rectangular boxes. Through the transition of the mid and late 15th Century, the semi-circular cover with cylindrical container became the popular style in the late 15th Century. In the late 16th Century, the style of the previous period was further developed to add some decorative elements, such as projecting ornaments, but the ornaments were added for functional purposes rather than artistic purposes. However, the style went back to the semi-circular cover with cylindrical container in the early 17th Century. From the mid 17th Century, various styles appeared with ornaments only on the cover, cone-shaped cover with no ornament, or mortar-like container. However, a new style of cone-shaped cover with ornament emerged between the early and mid 18th Century and continued to stay until the mid 18th Century. In the mid 18th Century, the cover remained unchanged, but the container became a keyhole-shaped space with one side forming a "ㄷ" shape. However, in the late 18th Century, the most typical style of the semi-circular cover with cylindrical container reappeared to show that this is the most universal style. Last, in the mid 19th Century, the cover changed from semi-circular to rectangular with flat top. After this, the taesil seokhams began to disappear. In terms of style, it can be classified into four stages. In the early Joseon Dynasty, the taesil seokhams were underdeveloped and inherited the style of the Goryeo Dynasty (Stage I; 1401~mid-15C), but Joseon's unique style began to develop from the late 15th Century (Stage II; 1477~1641). After that, partial ornaments were added for adornment (Stage III; 1660~1754), but the typical style reappeared in the late 18th Century to finally degrade in the late Joseon Dynasty of the mid 19th Century (Stage IV; 1790~1874). This arrangement of style and chronology would greatly help archaeologists anticipate the time and owner of taesil seokhams even if only taesil seokhams are discovered without any records. * Tae(胎) : Placenta and umbilical cord * Taesil(胎室) : A facility(chamber) of burying Tae(胎) in rite when royal descendants are born.