• Carbon letters
• /
• 제10권2호
• /
• pp.97-100
• /
• 2009

In this work, effects of carbon matrix on sliding friction and wear behavior of four kinds of C/C have been investigated against 40 Cr steel ring mate. Composite A with rough lamination carbon matrix (RL) shows the highest volume loss and coefficient of friction, while composite D with smooth lamination/resin carbon matrix (SL/RC) shows the lowest volume loss. The worn surface of composite A appears smooth, whereas that of composite C with smooth lamination carbon (SL) appears rough. The worn surface of composite D appears smooth under low load but rough under high load. Atomic force microscope images show that the size of wear particles on the worn surface is also dependent on the carbon matrix.

• Geomechanics and Engineering
• /
• 제18권2호
• /
• pp.133-140
• /
• 2019

Strain-rate and temperature have significant effects on the one-dimensional (1D) compression behavior of soils. This paper focuses on the bonding degradation effect of soil structure on the time and temperature dependent behavior of soft structured clay. The strain-rate and temperature dependency of preconsolidation pressure are investigated in double logarithm plane and a thermal viscoplastic model considering the combined effect of strain-rate and temperature is developed to describe the mechanical behavior of unstructured clay. By incorporating the bonding degradation, the model is extended that can be suitable for structured clay. The extended model is used to simulate CRS (Constant Rate of Strain) tests conducted on structural Berthierville clay with different strain-rates and temperatures. The comparisons between predicted and experimental results show that the extended model can reasonably describe the effect of bonding degradation on the stain-rate and temperature dependent behavior of soft structural clay under 1D condition. Although the model is proposed for 1D analysis, it can be a good base for developing a more general 3D model.

• Geomechanics and Engineering
• /
• 제18권1호
• /
• pp.71-83
• /
• 2019

This investigation presented conventional triaxial and creep-permeability tests on sandstones considering thermally-induced damage (TID). The TID had no visible effects on rock surface color, effective porosity and permeability below $300^{\circ}C$ TID level. The permeability enlarged approximately two orders of magnitude as TID increased to $1000^{\circ}C$ level. TID of $700^{\circ}C$ level was a threshold where the influence of TID on the normalized mass and volume of the specimen can be divided into two linear phases. Moreover, no prominent variations in the deformation moduli and peak strength and strain appeared as TID< $500^{\circ}C$ level. It is interesting that the peak strength increased by 24.3% at $700^{\circ}C$ level but decreased by 11.5% at $1000^{\circ}C$ level. The time-related deformation and steady-state creep rate had positive correlations with creep loading and the TID level, whereas the instantaneous modulus showed the opposite. The strain rates under creep failure stresses raised 1-4 orders of magnitude than those at low-stress levels. The permeability was not only dependent on the TID level but also dependent on creep deformation. The TID resulted in large deformation and complexity of failure pattern for the sandstone.

• Geomechanics and Engineering
• /
• 제25권3호
• /
• pp.195-205
• /
• 2021

Time effect on the deformation and strength characteristics of geogrid reinforced sand retaining wall has become an important issue in geotechnical and transportation engineering. Three physical model tests on geogrid reinforced sand retaining walls performed under various loading conditions were simulated to study their rate-dependent behaviors, using the presented nonlinear finite element method (FEM) analysis procedure. This FEM was based on the dynamic relaxation method and return mapping scheme, in which the combined effects of the rate-dependent behaviors of both the backfill soil and the geosynthetic reinforcement have been included. The rate-dependent behaviors of sands and geogrids should be attributed to the viscous property of materials, which can be described by the unified three-component elasto-viscoplastic constitutive model. By comparing the FEM simulations and the test results, it can be found that the present FEM was able to be successfully extended to the boundary value problems of geosynthetic reinforced soil retaining walls. The deformation and strength characteristics of the geogrid reinforced sand retaining walls can be well reproduced. Loading rate effect, the trends of jump in footing pressure upon the step-changes in the loading rate, occurred not only on sands and geogrids but also on geogrid reinforced sands retaining walls. The lateral earth pressure distributions against the back of retaining wall, the local tensile force in the geogrid arranged in the retaining wall and the local stresses beneath the footing under various loading conditions can also be predicted well in the FEM simulations.

• BMB Reports
• /
• 제40권5호
• /
• pp.723-730
• /
• 2007

Kinesin is an ATP-driven microtubule motor protein that plays important roles in control of microtubule dynamics, intracellular transport, cell division and signal transduction. The kinesin superfamily is composed of numerous members that are classified into 14 subfamilies. Animal kinesins have been well characterized. In contrast, plant kinesins have not yet to be characterized adequately. Here, a novel plant-specific kinesin gene, GhKCH2, has been cloned from cotton (Gossypium hirsutum) fibers and biochemically identified by prokaryotic expression, affinity purification, ATPase activity assay and microtubule-binding analysis. The putative motor domain of GhKCH2, $M_{396-734}$ corresponding to amino acids Q396-N734 was fused with 6$\times$His-tag, soluble-expressed in E. coli and affinity-purified in a large amount. The biochemical analysis demonstrated that the basal ATPase activity of $M_{396-734}$ is not activated by $Ca^{2+}$, but stimulated 30-fold max by microtubules. The enzymatic activation is microtubule-concentration-dependent, and the concentration of microtubules that corresponds to half-maximum activation was about 11 ${\mu}M$, much higher than that of other kinesins reported. The cosedimentation assay indicated that $M_{396-734}$ could bind to microtubules in vitro whenever the nucleotide AMP-PNP is present or absent. As a plant-specific microtubule-dependent kinesin with a lower microtubule-affinity and a nucleotide-independent microtubule-binding ability, cotton GhKCH2 might be involved in the function of microtubules during the deposition of cellulose microfibrils in fibers or the formation of cell wall.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권8호
• /
• pp.4147-4151
• /
• 2012

The current study was conducted to explore the inhibitory effects of a small interfering RNA (siRNA) on 3-phosphoinositide-dependent protein kinase 1 (PDK1) expression in esophageal cancer 9706 (EC9706) cells and the influence on their biological behavior. After transfection of a synthesized PDK1 siRNA, PDK1 mRNA and protein expression and the phosphorylation level of the downstream Akt protein were assessed using RT-PCR and Western blot analysis. Proliferation, apoptosis, cell invasion and in vivo tumor formation capacity were also investigated using MTT, flow cytometry, Transwell invasion trials, and nude mouse tumor transplantion, respectively. PDK1 siRNA effectively suppressed PDK1 mRNA and protein expression, and down-regulated the phosphorylation level of the Akt protein in the EC9706 cells (P < 0.05). It also inhibited cell proliferation and invasion, and promoted apoptosis; such effects were particularly obvious at 48 h and 72 h after transfection (P < 0.05). Growth of transplanted tumors was inhibited in nude mice, with decreased PDK1 expression in tumor tissues. PDK1 may be closely correlated with proliferation, apoptosis and invasion of esophageal cancer cells and thus may serve as an effective target for gene therapy.

• 전자공학회논문지CI
• /
• 제47권1호
• /
• pp.1-14
• /
• 2010

3D 콘텐츠 산업 분야의 급격한 성장과 더불어, 3D 콘텐츠 인증 및 신뢰, 검색을 위한 콘텐츠 해싱 기술이 요구되어지고 있다. 그러나 영상 및 동영상과 같은 2D 콘텐츠 해싱에 비하여 3D 콘텐츠 해싱에 대한 연구가 아직까지 미비하다. 본 논문에서는 키 기반의 3D 표면 계수 분포를 이용한 강인한 3D 메쉬 모델 해싱 기법을 제안한다. 제안한 기법에서는 기본적인 Euclid 기하학 변환에 강인한 3D SSD와 표면 곡률의 평면계 기반의 블록 표면 계수를 특징 벡터로 사용하며, 이를 치환 키 및 랜덤 변수 키에 의하여 최종 이진 해쉬를 생성한다. 실험 결과로부터 제안한 해싱 기법은 다양한 기하학 및 위상학 공격에 강인하며, 모델 및 키별로 해쉬의 유일성을 확인하였다.

• Experimental and Molecular Medicine
• /
• 제50권11호
• /
• pp.6.1-6.12
• /
• 2018

Morphine tolerance remains a challenge in the management of chronic pain in the clinic. As shown in our previous study, the dopamine D2 receptor (D2DR) expressed in spinal cord neurons might be involved in morphine tolerance, but the underlying mechanisms remain to be elucidated. In the present study, selective spinal D2DR blockade attenuated morphine tolerance in mice by inhibiting phosphatidylinositol 3 kinase (PI3K)/serine-threonine kinase (Akt)-mitogen activated protein kinase (MAPK) signaling in a ${\mu}$ opioid receptor (MOR)-dependent manner. Levo-corydalmine (l-CDL), which exhibited micromolar affinity for D2DR in D2/CHO-K1 cell lines in this report and effectively alleviated bone cancer pain in our previous study, attenuated morphine tolerance in rats with chronic bone cancer pain at nonanalgesic doses. Furthermore, the intrathecal administration of l-CDL obviously attenuated morphine tolerance, and the effect was reversed by a D2DR agonist in mice. Spinal D2DR inhibition and l-CDL also inhibited tolerance induced by the MOR agonist DAMGO. l-CDL and a D2DR small interfering RNA (siRNA) decreased the increase in levels of phosphorylated Akt and MAPK in the spinal cord; these changes were abolished by a PI3K inhibitor. In addition, the activated Akt and MAPK proteins in mice exhibiting morphine tolerance were inhibited by a MOR antagonist. Intrathecal administration of a PI3K inhibitor also attenuated DAMGO-induced tolerance. Based on these results, l-CDL antagonized spinal D2DR to attenuate morphine tolerance by inhibiting PI3K/Akt-dependent MAPK phosphorylation through MOR. These findings provide insights into a more versatile treatment for morphine tolerance.

• BMB Reports
• /
• 제40권2호
• /
• pp.172-179
• /
• 2007

PCR-mediated recombination describes the process of in vitro chimera formation from related template sequences present in a single PCR amplification. The high levels of genetic redundancy in eukaryotic genomes should make recombination artifacts occur readily. However, few evolutionary biologists adequately consider this phenomenon when studying gene lineages. The cytosolic glyceraldehyde-3-phosphate dehydrogenase gene (GapC), which encodes a NADP-dependent nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase in the cytosol, is a classical lowcopy nuclear gene marker and is commonly used in molecular evolutionary studies. Here, we report on the occurrence of PCR-mediated recombination in the GapC gene family of Hibiscus tiliaceus. The study suggests that recombinant areas appear to be correlated with DNA template secondary structures. Our observations highlight that recombination artifacts should be considered when studying specific and allelic phylogenies. The authors suggest that nested PCR be used to suppress PCRmediated recombination.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제8권10호
• /
• pp.3572-3590
• /
• 2014

Cellular automata (CA) based cryptosystem has been studied for almost three decades, yet most of previously reported researches focus on the symmetric key encryption schemes. Up to now, few CA based public key encryption scheme has been proposed. To fill the gap, in this paper, we propose a new public key encryption scheme based on layered cellular automata (LCA). Specifically, in the proposed scheme, based on the T-shaped neighborhood structure, we combine four one-dimensional reversible CAs (set as the private key) to form the transition rules of a two-dimension CA, where the two-dimension CA is set as the corresponding public key. Based on the hardness assumption of the Decisional Dependent CA problem in LCA, we formally prove the proposed scheme is indistinguishably secure against the chosen-plaintext attack (IND-CPA). In addition, we also use a numeric example to demonstrate its feasibility. Finally, analysis of key space and time efficiency are also carried out along with RSA-1024, and the simulation results demonstrate that our proposed scheme is more efficient.