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Dietary Supplementation with Acanthopanax senticosus Extract Modulates Gut Microflora in Weaned Piglets

  • Yin, Fugui;Yin, Yulong;Kong, Xiangfeng;Liu, Yulan;He, Qinghua;Li, Tiejun;Huang, Ruilin;Hou, Yongqing;Shu, Xugang;Tan, Liangxi;Chen, Lixiang;Gong, Jianhua;Kim, Sung Woo;Wu, Guoyao
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.9
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    • pp.1330-1338
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    • 2008
  • This study was conducted to investigate the effects of Acanthopanax senticosus extract (ASE) as a dietary additive on gut microflora in weaned piglets. A total of sixty pigs were weaned at 21 d of age (BW = $5.64{\pm}0.23kg$) and allocated on the basis of BW and litter to three dietary treatments in a randomized complete block design. The dietary treatments were: control group (basal diet), antibiotics group (basal diet+0.02% colistin), and ASE group (basal diet+0.1% ASE). On d 7, 14 and 28 after consuming the experimental diets, five piglets per group were sacrificed and then the contents from the jejunum, ileum and cecum were collected to determine changes in the microbial community by using a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique and estimating the contents of Lactobacillus and E. coli by in vitro culturing methods. The results showed that the ASE promoted the microflora diversity in the cecum. Enumeration of bacteria in the gut contents showed that the number of Lactobacillus increased (p<0.05), while that of E. coli decreased (p<0.05) when compared with the other 2 groups as the days of age progressed post-weaning. These findings suggested that the ASE, as a substitute for dietary antimicrobial products, could improve the development of the normal gut microflora and suppress bacterial pathogens, and effectively promote a healthy intestinal environment.

Association of UDP-galactose-4-epimerase with milk protein concentration in the Chinese Holstein population

  • Li, Cong;Cai, Wentao;Liu, Shuli;Zhou, Chenghao;Cao, Mingyue;Yin, Hongwei;Sun, Dongxiao;Zhang, Shengli;Loor, Juan J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.11
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    • pp.1725-1731
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    • 2020
  • Objective: An initial RNA-Sequencing study revealed that UDP-galactose-4-epimerase (GALE) was one of the most promising candidates for milk protein concentration in Chinese Holstein cattle. This enzyme catalyzes the interconversion of UDP-galactose and UDP-glucose, an important step in galactose catabolism. To further validate the genetic effect of GALE on milk protein traits, genetic variations were identified, and genotypes-phenotypes associations were performed. Methods: The entire coding region and the 5'-regulatory region (5'-UTR) of GALE were re-sequenced using pooled DNA of 17 unrelated sires. Association studies for five milk production traits were performed using a mixed linear animal model with a population encompassing 1,027 Chinese Holstein cows. Results: A total of three variants in GALE were identified, including two novel variants (g.2114 A>G and g.2037 G>A) in the 5'-UTR and one previously reported variant (g.3836 G>C) in an intron. All three single nucleotide polymorphisms (SNPs) were associated with milk yield (p<0.0001), fat yield (p = 0.0006 to <0.0001), protein yield (p = 0.0232 to <0.0001) and protein percentage (p<0.0001), while no significant associations were detected between the SNPs and fat percentage. A strong linkage disequilibrium (D' = 0.96 to 1.00) was observed among all three SNPs, and a 5 Kb haplotype block involving three main haplotypes with GAG, AGC, and AGG was formed. The results of haplotype association analyses were consistent with the results of single locus association analysis (p<0.0001). The phenotypic variance ratio above 3.00% was observed for milk protein yield that was explained by SNP-g.3836G >C. Conclusion: Overall, our findings provided new insights into the polymorphic variations in bovine GALE gene and their associations with milk protein concentration. The data indicate their potential uses for marker-assisted breeding or genetic selection schemes.

Effect of Shear Key and Edge Length of Near Surface-Mounted FRP Plate in Concrete (콘크리트에 표면매입 보강된 FRP판의 전단키 및 연단거리 효과)

  • Seo, Soo-Yeon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.20 no.1
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    • pp.41-47
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    • 2016
  • This paper presents a bond test to find the effect of shear key and edge length from the bonded FRP in near surface-mounted(NSM) retrofit using FRP plate. Main parameters in the test are the location and size of shear key and the edge length. For the test, 10 specimens were made by embedding FRP plate of $3.6mm{\times}16mm$ into $400mm{\times}200(300)mm{\times}400mm$ concrete block and fixing it by using epoxy. Tensile load was applied to the FRP of the specimens until failure and was recorded at each load increase. In addition, the bond slip and elongation of FRP were measured during the test. From the test, it was found that the further the shear key located from the loading, the higher strength we could get. The bond strength inversely depended on the size of shear key. Especially, when the size of shear key was to be lagger than certain size, the bond strength decreased to very low value; even less than that of the case without shear key. The bond strength somewhat increased corresponding to the increase of edge length from the bonded end of FRP to loading in spite of same bond length. The bond-slip between FRP and concrete governed overall deformation in the bond test of NSM FRP so that the effect of excessive slip is necessary to be considered in the design.

Fast Motion Estimation for Variable Motion Block Size in H.264 Standard (H.264 표준의 가변 움직임 블록을 위한 고속 움직임 탐색 기법)

  • 최웅일;전병우
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.41 no.6
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    • pp.209-220
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    • 2004
  • The main feature of H.264 standard against conventional video standards is the high coding efficiency and the network friendliness. In spite of these outstanding features, it is not easy to implement H.264 codec as a real-time system due to its high requirement of memory bandwidth and intensive computation. Although the variable block size motion compensation using multiple reference frames is one of the key coding tools to bring about its main performance gain, it demands substantial computational complexity due to SAD (Sum of Absolute Difference) calculation among all possible combinations of coding modes to find the best motion vector. For speedup of motion estimation process, therefore, this paper proposes fast algorithms for both integer-pel and fractional-pel motion search. Since many conventional fast integer-pel motion estimation algorithms are not suitable for H.264 having variable motion block sizes, we propose the motion field adaptive search using the hierarchical block structure based on the diamond search applicable to variable motion block sizes. Besides, we also propose fast fractional-pel motion search using small diamond search centered by predictive motion vector based on statistical characteristic of motion vector.

A Study on the Reasonable Application Method of Steel Fiber Reinforced Shotcrete in NATM Tunnel (NATM 터널에서 합리적인 강섬유보강 숏크리트 적용방안에 관한 연구)

  • Seok, Chongken;Ahn, Taebong
    • Journal of the Korean GEO-environmental Society
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    • v.15 no.9
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    • pp.19-28
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    • 2014
  • Recently, the proportion of long tunnel has increased for efficient use of land due to limited land area, driving convenience and high speed. RMR and Q-System of empirical methods has been mainly used for tunnel support design in domestic. Although shotcrete is the key to NATM tunnel, the related studies are insufficient. So, steel fiber reinforced shotcrete is applied to II~V grade rocks on domestic and foreign applications. And same amount of shotcrete is equally applied to tunnel roof and wall regardless of the applied rock load. Shotcrete is applied excessively rather than the original proposed value of RMR and Q-System. Thus, this study is to reevaluate the application part of plain shotcrete and steel fiber reinforced shotcrete of tunnel in Daebo granite, and to propose the reasonable application method of steel fiber reinforced shotcrete. Field test and numerical back analysis using measurements were performed to verify stability. According to results, if RMR values are the upper class in the III grade, it can be designed in accordance with upper grade. In addition, if rock condition is good as a mountain tunnel in Daebo granite, it can be applied for plain shotcrete to III grade rocks because there is also no stability problems. And although steel fiber reinforced shotcrete is applied only crown of the tunnel in IV grade rocks, it is possible to secure stability for falling rock by key-block.

Comparisons of Certification Standards for Mask and Review on Filtration Efficiency for Viruses (마스크의 인증기준 비교와 바이러스 여과효율에 대한 고찰)

  • Yoon, Chungsik;Go, Sulbee;Park, Jihoon
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.30 no.2
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    • pp.109-123
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    • 2020
  • Objectives: The aims of this study were to review the standards and key components of the standards for disposable masks in Korea, the US, EU, Japan, and China and to evaluate the appropriateness of disposable masks during a virus pandemic. Methods: We reviewed the standards in the above countries and compared their key elements for each standard. For the second purpose, systemic paper gathering using key words like 'mask', 'respirator' 'virus', and 'coronavirus' in the PubMed search engine was performed. Fifty-three papers were selected and reviewed in regard to the appropriateness of test protocols with sodium chloride(NaCl) particles for virus filtration and the effectiveness against viruses. Results: The standards for masks are largely divided into two categories: US standards and EU standards. In Korea, the Ministry of Employment and Labor adapted the EU standards for workers and the Health Masks adopted the Ministry of Employment and Labor standards by the Ministry of Food and Drug Safety. Regarding airborne viral infections, WHO emphasizes only droplet infection, while many studies have shown that small particles enter the air through coughing or sneezing, which increases the possibility of airborne infection. Compared to other particles, various factors such as airborne viability and the ability to replicate the virus in the body are further involved in the virus's airborne infection rate. Airborne infection is classified into absolute air infection, preferential air infection, and opportunistic air infection. The NaCl-certified N95 mask showed good filtration efficiency against viruses and NaCl particles were proved to be a surrogate material for viruses. From this, KF94 is also expected to be effective in blocking viruses. Conclusion: The N95 test method could be used as a surrogate test method for virus filtration. N95-class masks have been found to effectively block viral infections in the air. However, surgical or medical masks are only partially effective against airborne virus infection though they could effectively block large droplet infection. However, most studies considered in this study targeted N95 in foreign countries and studies on masks actually used in Korea are very limited, so studies on microorganisms and reuse on domestic masks should be conducted in the future.

Assessment of mulberry leaf as a potential feed supplement for animal feeding in P.R. China

  • Cai, Ming;Mu, Lan;Wang, Zong-li;Liu, Jian-yong;Liu, Tian-liang;Wanapat, Metha;Huang, Bi-zhi
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.8
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    • pp.1145-1152
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    • 2019
  • Objective: Mulberry (Morus alba L.) is a cultivated shrub grown widely in the sub-tropical and tropical areas. It has been shown that mulberry leaf contains high levels of protein while having polyphenols as phytonutrients. Therefore, it is important to conduct an experiment to assess potential toxic level from mulberry on behavior, blood hematological and coagulation parameter using Sprague-Dawley (SD) rats. Methods: Both male and female SD rats were given an intragastric administration of respective treatments of mulberry leaf intakes (control, low and high levels). Parameters of feed intake, hematological and coagulation of blood parameters, as well as liveweight changes were taken during the 7 d of adaptation, 28 d of treatment exposure, and 14 d of recovery periods, respectively. All treatment data were statistically analyzed using analysis of variance of SPSS17.0 for Windows Statistical Software following the Randomized complete block design with sex as a block. Results: Most of the parameters of the physical symptoms of the SD rats, were not significantly different (p>0.05) when compared with that of the control group. Those which remain unchanged in each dose group were, body weight (BW) gain, feed intake, the hematology and coagulation indexes. Although, there were a few individual indicators that were abnormal, but the overall physiological appearance of the rats were normal. Conclusion: Results under this experiment revealed that most hematological and coagulation parameters of the SD rats in both male and female were normal, although the weight gain of female rats in high-dose group was significantly reduced than those of the male rats. Under this study, the use of mulberry leaf up to 2 g/kg BW did not result in abnormal phenomenon in the SD rats. These findings would offer useful information for further in vivo feeding trials in animals to extensively use of mulberry leaf to improve animal production, particularly in P.R. China.

S-PRESENT Cryptanalysis through Know-Plaintext Attack Based on Deep Learning (딥러닝 기반의 알려진 평문 공격을 통한 S-PRESENT 분석)

  • Se-jin Lim;Hyun-Ji Kim;Kyung-Bae Jang;Yea-jun Kang;Won-Woong Kim;Yu-Jin Yang;Hwa-Jeong Seo
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.33 no.2
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    • pp.193-200
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    • 2023
  • Cryptanalysis can be performed by various techniques such as known plaintext attack, differential attack, side-channel analysis, and the like. Recently, many studies have been conducted on cryptanalysis using deep learning. A known-plaintext attack is a technique that uses a known plaintext and ciphertext pair to find a key. In this paper, we use deep learning technology to perform a known-plaintext attack against S-PRESENT, a reduced version of the lightweight block cipher PRESENT. This paper is significant in that it is the first known-plaintext attack based on deep learning performed on a reduced lightweight block cipher. For cryptanalysis, MLP (Multi-Layer Perceptron) and 1D and 2D CNN(Convolutional Neural Network) models are used and optimized, and the performance of the three models is compared. It showed the highest performance in 2D convolutional neural networks, but it was possible to attack only up to some key spaces. From this, it can be seen that the known-plaintext attack through the MLP model and the convolutional neural network is limited in attackable key bits.

Efficient FPGA Implementation of AES-CCM for IEEE 1609.2 Vehicle Communications Security

  • Jeong, Chanbok;Kim, Youngmin
    • IEIE Transactions on Smart Processing and Computing
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    • v.6 no.2
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    • pp.133-139
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    • 2017
  • Vehicles have increasingly evolved and become intelligent with convergence of information and communications technologies (ICT). Vehicle communications (VC) has become one of the major necessities for intelligent vehicles. However, VC suffers from serious security problems that hinder its commercialization. Hence, the IEEE 1609 Wireless Access Vehicular Environment (WAVE) protocol defines a security service for VC. This service includes Advanced Encryption Standard-Counter with CBC-MAC (AES-CCM) for data encryption in VC. A high-speed AES-CCM crypto module is necessary, because VC requires a fast communication rate between vehicles. In this study, we propose and implement an efficient AES-CCM hardware architecture for high-speed VC. First, we propose a 32-bit substitution table (S_Box) to reduce the AES module latency. Second, we employ key box register files to save key expansion results. Third, we save the input and processed data to internal register files for secure encryption and to secure data from external attacks. Finally, we design a parallel architecture for both cipher block chaining message authentication code (CBC-MAC) and the counter module in AES-CCM to improve performance. For implementation of the field programmable gate array (FPGA) hardware, we use a Xilinx Virtex-5 FPGA chip. The entire operation of the AES-CCM module is validated by timing simulations in Xilinx ISE at a speed of 166.2 MHz.

Data-Hiding for Halftone Images Using an Improved CPT scheme

  • Phan, Trung Huy;Nguyen, Hai Thanh;Kim, Cheonshik;Yang, Ching-Nung
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.7 no.2
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    • pp.405-424
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    • 2013
  • In publishing applications, it is advantageous to embed data in halftone images. The CPT scheme (Chen-Pan-Tseng, 2000) is a steganographic data hiding scheme that was proposed for binary images, e.g., facsimiles. The CPT scheme uses a secret key and weight matrix to protect the hidden data and can hide as many as $r={\lfloor}{\log}_2(m{\times}n+1){\rfloor}$ bits of data in the image by changing at most 2 bits in the image. Our proposed scheme also uses a secret key to protect it from being compromised and a weight matrix to increase the data hiding rate. Our scheme improves the performance of the CPT scheme by using the simple principle of splitting each block into two parts. Our proposed scheme is called improved CPT (ICPT) and has a very high embedding capacity compared to previous schemes. Experimental results demonstrate that our proposed scheme generally has higher performance than previous schemes.