• Title/Summary/Keyword: karyotypes

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The Karyotype of a Riceash, Oryzias latipes from Southern Korea (한국 남부지방에 서식하는 송사리 Oryzias latipes (Temminck et Schlegel)의 핵형)

  • 김익수;문교정
    • The Korean Journal of Zoology
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    • v.30 no.4
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    • pp.379-386
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    • 1987
  • Karyotype of the ricessh Orygias latipes (Temminck et Schlegel) was investigated using 151 specimens of eleven populations in the southern Korea. Based on the karyotype analysis, two distinct chromosomal groups were found. One group, occurring in the Kum River, the Mankyong River, the Dongjin River, the Ysngsan River and the SEmjin River had a diploid chromosome number of 46 and arm number of 70, which contained one pair of "large" metacentric chromosomes. The other group, occurring in the Tamjin River, the Hyongsan River andthe all four island populations observed had a diploid chromosome number of 48 and arm number of 68. These results showed that 0. latipes of 6 inland populations was identical with the Chinese population in diploid number, arm number and presence of "large" metacentric chromosome, while the present species of island populations ana populations of the Tamjn R. and Hyongsan R. was similar to the Japanese population in chromosome number and arm number. The karyotype variation in populations indicated that karyotypes will probably provide useful information in studying zoogeography of this species.phy of this species.

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Karyotype of Korean Birds VI. Karyological Anaiysis on Two Species of the Genus Dendrocopos by C-Banding Method (한국산 조류의 핵형 VI. C-Banding방법에 의한 딱다구리의 두 종의 핵형분석)

  • 이혜영;이성근;유성림
    • The Korean Journal of Zoology
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    • v.33 no.2
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    • pp.217-221
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    • 1990
  • The chromosomal analyses of Dendmcopos major hondoensis and D. leucotos Ieucotos(Picidae:Piciformes) in Korea were performed by conventional Giemsa staining and C-banding method. The diploid number of two species was 2n=90-92 and arm number was AN= 92-96.The conventional karyotypes were very similiar but distribution of constitutive heterochromatin were differ in the first chromosome. The second and several pairs of macro-telocentric chromosbmes have telomeric constitutive heterochromatin.

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Taxonomic Study on Spider Mites (Tetranychidae ; Acarina ) of Korea (한국산 응애과 (거미속 : 진드기목) 의 분류학적 연구)

  • 이원구;이병훈;김영진
    • Animal Systematics, Evolution and Diversity
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    • v.2 no.2
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    • pp.13-26
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    • 1986
  • A Taxonomic investigation was carried out on the following seven spider mites (Family tetranychidae) by comparison of morphological characters, cuticular lobes and karyotypes. Materials used in this study were collected and reared at Jeonju, Jorea during October 1985-June 1986. Subfamily Bryobiinae ; Bryobia praetiosa Koch, 1836 ; Petrobia latens (Mller, 1776). Subfamily Tetranychinae ; Panonychus citri (McGregor , 1919) ; P.ulmi (Koch, 1836): T vienensis tranychus Zacher , 1920 ; T.urticae (Koch, 1836) ; T. kanzawai Kishida , 1927. Of them two species, P. latens and T. kanzawai , are newly recorded from Korea.

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Monosomal Karyotypes among 1147 Chinese Patients with Acute Myeloid Leukemia: Prevalence, Features and Prognostic Impact

  • Yang, Xiao-Fei;Sun, Ai-Ning;Yin, Jia;Cai, Cheng-Sen;Tian, Xiao-Peng;Qian, Jun;Chen, Su-Ning;Wu, De-Pei
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.11
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    • pp.5421-5426
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    • 2012
  • A monosomal karyotype (MK), defined as ${\geq}2$ autosomal monosomies or a single monosomy in the presence of additional structural abnormalities, was recently identified as an independent prognostic factor conveying an extremely poor prognosis in patients with acute myeloid leukemia (AML). In the present study, after excluding patients with t(15;17), t(8;21), inv(16) and normal karyotypes, 324 AML patients with cytogenetic abnormalities were the main subject of analysis. The incidences of MK were 13% in patients aged 15 to 60 years and 18% in those between 15 and 88 years old. MK was much more prevalent among elderly patients (p < 0.001) and was significantly associated with the presence of -7, -5, del(5q), abn12p, abn17p, -18 or 18q-, -20 or 20q- and CK (for all p < 0.001 except for abn12p p=0.009), and +8 or +8q was less frequent in MK+ AML(p=0.007). No correlation was noted between monosomal karyotype and FAB subtype (p > 0.05); MK remained significantly associated with worse overall survival among patients with complex karyotype (p=0.032); A single autosomal monosomy contributed an additional negative effect in OS of patients with structural cytogenetic abnormalities (P=0.008). This report presents the prevalence, feature and prognostic impact of MK among a large series of Chinese AML patients from a single center for the first time.

Cytogenetic Analysis of Seven Angelica Species (Angelica속 식물 7종의 세포유전학적 분석)

  • Choi, Hae-Woon;Koo, Dal-Hoe;Lee, Woo-Kyu;Kim, Soo-Young;Sung, Jung-Sook;Seong, Nak-Sul;Suh, Young-Bae;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.3
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    • pp.118-121
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    • 2005
  • Karyotypes were established in seven Angelica species cultivated in Korea. The somatic chromosome numbers were 2n = 2x = 22 with the basic number of x = 11 in all Angelica plants examined. Their metaphase chromosomes ranged from 3.56 ${\mu}M$. to 8.91 x. in length. Distinctive Karyotypes were found in two species, A. tenuissima with all metacentries, K(2n) = 2x = 22m, and A. genuflexa with all subtelocentrics, K(2n) = 2x = 22st. Karyotype formulas of A. gigas, A. acutiloha, A. sinensis, A. decursiva and A. dahurica were K(2n) = 2x = 20m + 2sm, K(2n) = 2x = 12m + 10sm, K(2n) = 2x = 16m + 6sm, K(2n) = 2x = 18m + 4sm and K(2n) = 2x = 10m + 10sm + 2st, respectively. Cytological data showed that chromosomal polymorphisms within species were observed in Angelica plants compare to other regions.

A cytotaxonomic study of Vicia L. (Fabaceae) in Korea (한국산 나비나물속(콩과)의 세포분류학적 연구)

  • Nam, Bo Mi;Park, Myung Soon;Oh, Byoung Un;Chung, Gyu Young
    • Korean Journal of Plant Taxonomy
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    • v.42 no.4
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    • pp.307-315
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    • 2012
  • Somatic chromosome numbers for 10 taxa and karyotypes analysis for 6 taxa of Korean Vicia were investigated. Somatic chromosome numbers of treated taxa were 2n = 12, 14 or 24 and therefore they proved to be diploid or tetraploid with basic chromosome numbers of x = 6 or 7. The chromosome number of V. hirticalycina (2n = 2x = 12) was reported for the first time in this study. The chromosome numbers of nine taxa were the same as in previous studies; V. angustifolia (2n = 2x = 12), V. cracca (2n = 4x = 24), V. hirsuta (2n = 2x = 14), V. tetrasperma (2n = 2x = 14 + 2B), V. amurensis (2n = 2x = 12), V. chosenensis (2n = 2x = 12, 12 + 2B), V. unijuga (2n = 4x = 24), V. unijuga f. minor (2n = 4x = 24), V. venosa var. cuspidata (2n = 4x = 24). The karyotypes of V. cracca, V. amurensis, V. hirticalycina, V. unijuga, V. unijuga f. minor, V. venosa var. cuspidata were observed as 2 m + 8 sm + 2 st, 2 m + 2 sm + 2 st, 3 m + 1 sm + 2 st, 4 m + 6 sm + 2 st, 4 m + 6 sm + 2 st, 4 m + 8 sm, respectively.

Karyotype and Reproductive Characteristics of the Diploid Brackish Water Clam, Corbicula japonica and the Triploid Freshwater Marsh Clam, C. fluminea (2배체 기수산 일본재첩 Corbicula japonica와 3배체 담수산 재첩 C. fluminea의 핵형분석 및 번식 특성)

  • Choi, Ki-Ho;Chung, Ee-Yung;Kwak, Oh-Yeol
    • The Korean Journal of Malacology
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    • v.23 no.1
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    • pp.39-49
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    • 2007
  • We investigated to understand the differences in the karyotypes and the reproductive characteristics between the diploid clam, Corbicula japonica in the brackish water and the triploid clam, C. fluminea in the freshwater. The number of chromosomes of the diploid C. japonica were 38, which can be grouped into 19 pairs of two homologues. These can be divided into 11 pairs of telocentric, 4 pairs of subtelocentric, 3 pairs of submetacentric and one pair of metacentric chromosomes. C. japonica is oviparous and dioecious. The clam has two pairs of gills, with the inner and outer-demibranchs, however, they do not act mainly as marsupia. External fertilization occurs in the brackish water. The triploid marsh clam, C. fluminea has 54 chromosomes, which can be grouped into 18 sets of three homologues. These 18 sets can be divided into one metacentric group, five submetacentric groups and 12 subtelo- or telocentric groups. C. fluminea is ovoviviparous and functional hermaphrodite. The triploid condition may be closely related to hermaphroditism. This species has the inner-demibranchs acting mainly as marsupia. The depletion of ripe eggs occurred in the inner-demibranchs of the gill during the incubatory periods, and ripe eggs produced in the hermaphroditic follicles in the gonad during the non-incubatory periods. The gonad developmental stages of two marsh clams (C. japonica and C. fluminea) can be divided into five stages: early active stage, late active stage, ripe stage, partially spawned stages, and spent/inactive stage.

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Ten-year Clinical Study of Chorionic Villus Sampling (융모막 융모생검 511례에 대한 임상적 연구: 10년(2000-2010년)간의 경험)

  • Kim, Soo-Hyun;Shim, Sung-Han;Baek, Jong-Woo;Cha, Dong-Hyun
    • Journal of Genetic Medicine
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    • v.8 no.1
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    • pp.35-43
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    • 2011
  • Purpose: We evaluated indications for chorionic villus sampling (CVS), the positive predictive value of CVS for fetal chromosomal abnormalities, and the fetal loss rate after CVS at CHA Medical Center. Materials and Methods: We reviewed the medical records of 511 cases of CVS performed between 67 and 120 days of gestation for prenatal cytogenetic diagnosis from April 2000 to April 2010. Fetal karyotypes were obtained by direct and indirect culture methods. Results: The most common indications for CVS were abnormal ultrasonic findings including increased nuchal translucency (294/635, 46.3%). The positive predictive value of abnormal karyotyping according to indication for CVS was highest in cases with abnormal parental karyotypes (14/21, 66.7%). Mosaicism revealed by CVS comprised 3.1% of the sample (16/509). Amniocentesis revealed two cases of true mosaicism and 11 cases of confined placental mosaicism. The fetal loss rate within 4 weeks of the procedure was 1.2% (6/511). Conclusion: If CVS is performed by an expert clinician, it is a feasible and reliable procedure for prenatal genetic diagnosis. When CVS indicates mosaicism, the finding should be confirmed by amniocentesis to distinguish true mosaicism from confined placental mosaicism.

Prevalence of Balanced Chromosomal Translocations in Couples with Abnormal Reproductive Outcomes and Prenatal Cytogenetic Diagnosis in the Carriers (비정상 산과력을 가진 부부에서의 균형전좌형 염색체 보인자의 빈도 및 그 보인자들에서의 산전 세포유전학적 진단)

  • Part, So-Yeon;Kang, Inn-Soo;Ryu, Hyun-Mee;Jun, Jong-Young;Lee, Moon-Hee;Kim, Jin-Mi;Choi, Soo-Kyung
    • Clinical and Experimental Reproductive Medicine
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    • v.24 no.3
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    • pp.393-398
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    • 1997
  • Cytogenetic analysis was performed in 1321 couples and 141 women with history of abnormal reproductive outcome during 1988-1996. The use of high resolution banding technique and fluorescence in situ hybridization (FISH) in the chromosome analysis has made the precise evaluation of chromosome aberrations. The prevalence of balanced chromosomal translocation carriers were 3.74% (104/2783 patients). 70 cases (2.52%) were reciprocal translocation carriers and 34 (1.22%) had Robertsonian translocations. Chromosome aberrations were more frequent in women (73 cases) than in men (31 cases). No phenotypical abnormalities were found in all carriers, but they experienced abnormal reproductive outcomes such as recurrent spontaneous abortions, anomalous offsprings or infertility problem. Prenatal diagnosis was carried out on 36 subsequent pregnancies in balanced translocation carriers. The fetal karyotypes showed that 12 cases (33%) were normal, 22 (61%) were balanced translocations, and two (6%) were unbalanced translocations. It is concluded that the prevalence of balanced chromosomal translocations in patients with abnormal reproductive outcome is higher than that of the normal population. Most of the fetal samples showed normal karyotypes or balanced translocations. Although the incidence of chromosomal imbalance in the fetuses was relatively low in prenatal diagnosis, individuals with balanced translocations are predisposed to abnormal offspring with partial trisomy or monosomy. Therefore we recommend that genetic counselling and cytogenetic prenatal diagnosis for translocation carriers have to be offered to prevent recurrent chromosomal abnormal babies.

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Cytogenetic Analysis of Spotty Belly Greenling (Hexagrammos agrammus) and Greenling (H. otakii) (노래미 (Hexagrammos agrammus)와 쥐노래미 (H. otakii)의 세포유전학적 연구)

  • Sim Mi A;Noh Jae Koo;Nam Yoon Kwon;Kim Dong Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.6
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    • pp.682-685
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    • 2002
  • Cytogenetic analysis was conducted to obtaining informations for genetic improvement of spotty belly greenling (Hexagrmmos agrammus) and greenling (H. otakii) in aquaculture. Erythrocytes of spotty belly greenling were slightly larger than those of greenling (p<0.05). The nuclear volume of spotty belly greening erythrocytes averaged 15.14 $\pm$ 0.92 ${\mu}m^3$ while that of greening averaged 14.61 $\pm$ 0.15 $\mu$m^3 the difference was not significant (p>0.05). Consequently, genome size of spotty belly greenling was also slightly larger than those of greenling. DNA content per cell of spotty belly greenling and greenling were 2.15 pg and 2.10 pg, respectively. The modal chromosome number of both greenling species were same as 2n=48 and karyotypes were also identical as 2 metacentrics, 11 snbrnetacentrics and 11 acrocentric pairs $(W: 74), There was no evidence of polymorphism including aneuploidy or sex-related heterornorphisrn for all specimens examined. The nuclear organizer regions (NOR_s)$ were localized on a small acrocentric chromosome pair in both species, Spotty belly greenling showed large sizes of active rRNA coding regions in their chromosomes. However, greenling examined only small sizes of active rRNA coding regions with dimorphism.