• 한국식품과학회지
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• 제40권1호
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• pp.118-121
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• 2008

본 연구는 소금의 첨가수준을 저수준(1.5%)으로 고정시키고, 인산염 대체가능성이 있는 다양한 기능성 소재들의 수준을 달리하여 원료돈육의 가공특성, 유화안정성 및 육색 등에 미치는 첨가효과를 구명하였다. 기능성 물질의 첨가는 원료돈육의 일반성분과 pH에는 유의적인 영향을 미치지 않았다. 보수력과 가열 감량에 있어 후보 물질들과 인산염 처리구 간에 유의적인 변화가 나타났고, 구아검, 카라기난, 알긴산이 인산염 대체에 적적한 특성을 보였다. 유화안정성에서는 인산염, 알긴산과 구아검 첨가구가 유의적으로 높은 안정성을 나타냈어 이후 실제 가공품에의 적용가능성을 나타냈다. 이상의 결과에서 카라기난과 구아검의 경우인산염과 유사한 가공특성, 유화안정성과 육색을 나타내어 육제품 제조용 인산염 대체첨가물질로 가능성을 확인할 수 있었다.

• Parasites, Hosts and Diseases
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• 제33권1호
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• pp.45-54
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• 1995

한국산 Cryptosporidium baileyi를 2일령의 각 청둥오리와 병아리에 $2{\;}{\times}{\;}10^6$개의 오오시스트와 그 10배단계 희석계열을 $2{\;}{\times}{\;}10^2$까지 5단계를 만들어 경구접종시켜 오오시스트 배설상황과 여러 장기내 발육기의 분포상황을 조사하고나서 초감염내과 조류에 처음에 40 mg/30 g 그 24시간 후에 10 mg/30 g의 carrageenan을 복강내 투여하고 그 6시간 후에 다시 $2{\;}{\times}{\;}10^6$개의 오오시스트를 재차접종하여 면역원성을 조사하였다. 오오시스트 투여량이 많을수록 patent period가 길었고(27-30일간, 병아리: 10-18일간 청둥오리), 오오시스트 배설량도 많았으나(극기에 있어서 $6{\;}{\times}{\;}10^5-3.5{\;}{\times}{\;}10^6$, 병아리: $2{\;}{\times}{\;}10^5-4{\;}{\times}{\;}10^5$, 청둥오리) prepatent period는 짧았다(2-3일간. 병아리: 3-4일간, 청둥오리). 한편, 청둥오리에서는 $2{\;}{\times}{\;}10^3{\;}및{\;}2{\;}{\times}{\;}10^3$의 오오시스트 접종군에서는 오오시스트가 전혀 검출되지 않았다. 오오시스트 경구접종 후 12일에 총배설강과 파브리시우스낭에서 다수의 발육기를 검출할 수 있었는데 병아리가 청둥오리보다 현저하였다. Carrageenan 투여군은 모두 대조군은 병아리에서 $2{\;}{\times}{\;}10^5{\;}및{\;}2{\;}{\times}{\;}10^3$, 그리고 청둥오리에서 이 밖에 $2{\;}{\times}{\;}10^4$의 오오시스트 투여군에서도 재차 접종후 4일에서 14일 및 5일에서 15일까지 분변으로부터 오오시스트가 다소 검출되었다 병아리 및 청둥오리에 있어서 재차접종 직전의 말초혈액내 대식세포수 및 복강내 대식세포 활성은 대조군에 비하여 carrageenan 투여군이 현저하게 낮았다(P < 0.05) 병아리와 청둥오리는 Meblocker인 carrageenan을 투여하였음에도 불구하고 이 원충의 재차감염이 미약하였다는 것은 이 원충의 면역원성이 극히 강력하였다는 것을 뜻한다.

• 약학회지
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• 제44권6호
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• pp.588-594
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• 2000

To investigate the effects of iota-carrageenan (CAR) and/or alum on the adjuvancity as well as the structural difference of oligosaccharide on the IgG2b in the adjuvant effect, C57BL/6 mice were immunized twice with fetuin as a model antigen. CAR alone showed no significant effect on induction of antibody except IgG1. In contrast, Alum-CAR (after mixing of antigen-Alum, CAR adjuvant was prepared) and CAR-Alum (after formulation of antigen-CAR, Alum adjuvant was prepared) enhanced production of antibody, especially, IgG2b. After separation of IgG2b, changes of glycosylation were investigated using enzymelinked lectin assay. High affinity of IgG2b to N-acetylneuraminic acid, galactose and mannose-specific lectin were induced by CAR-Alum adjuvant, however, the affinity of IgG2b induced by CAR-Alum to GlcNAc and GalNAc-specific lectin were much less than that induced by Alum-CAR.

• 한국산학기술학회논문지
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• 제21권3호
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• pp.215-222
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• 2020

메트포르민은 제2형 당뇨병의 1차 치료제로 사용되는 약물로 다른 당뇨병 치료제에 비해 투여 용량이 크고 용해도는 높으나 위장관 투과도가 낮은 특성을 갖고 있으며 주로 위장관 상부에서만 흡수되는 이유로 생체이용률이 40~60%로 낮은 편이다. 따라서 본 연구를 통해 위체류 약물전달시스템을 적용하여 제제가 위에 머무르는 시간을 증가시키고 제제로부터 방출된 약물을 서서히 소장으로 이동시킨다면 생체이용률을 증가시킬 수 있을 것으로 기대된다. 팽윤성 시스템은 다른 위체류 약물전달시스템에 비해 안정성이 높아 개발목표기술로 선정하였고, 팽윤성 기제로는 기존의 연구와 다르게 카라기난과 히프로멜로오스를 병용하여 차별성을 확보하였다. 카라기난과 히프로멜로오스 병용 시스템은 각각의 함량이 15/110 질량분율일 때 가장 높은 팽윤성과 적절한 서방성 용출패턴을 나타내었다. 또한 각각의 함량이 15 %와 14 %가 되도록 제조한 메트포르민 정제를 시판 정제와 비교 시 더 우수한 팽윤성이 나타남을 확인하였다. 결론적으로 본 연구 결과에 의해 메트포르민의 서방출을 위한 새로운 팽윤성 위체류 약물전달시스템이 개발되었으며 다양한 주성분에 대한 추가적인 연구가 수행되면 의약품, 화장품, 건강기능식품 등의 분야에서 효과적인 약물전달시스템으로 응용될 수 있을 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• 제18권10호
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• pp.1641-1647
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• 2008

Amygdalin is a cyanogenic glycoside plant compound found in the seeds of rosaceous stone fruits. We evaluated the anti-inflammatory and analgesic activities of amygdalin, using an in vitro lipopolysaccharide (LPS)-induced cell line and a rat model with carrageenan-induced ankle arthritis. One mM amygdalin significantly inhibited the expression of TNF-$\alpha$ and IL-l$\beta$ mRNAs in LPS-treated RAW 264.7 cells. Amygdalin (0.005, 0.05, and 0.1 mg/kg) was intramuscularly injected immediately after the induction of carrageenan-induced arthritic pain in rats, and the anti-arthritic effect of amygdalin was assessed by measuring the weight distribution ratio of the bearing forces of both feet and the ankle circumference, and by analyzing the expression levels of three molecular markers of pain and inflammation (c-Fos, TNF-$\alpha$, and IL-l$\beta$) in the spinal cord. The hyperalgesia of the arthritic ankle was alleviated most significantly by the injection of 0.005 mg/kg amygdalin. At this dosage, the expressions of c-Fos, TNF-$\alpha$, and IL-l$\beta$ in the spinal cord were significantly inhibited. However, at dosage greater than 0.005 mg/kg, the pain-relieving effect of amygdalin was not observed. Thus, amygdalin treatment effectively alleviated responses to LPS-treatment in RAW 264.7 cells and carrageenan-induced arthritis in rats, and may serve as an analgesic for relieving inflammatory pain.

• 한국식품영양과학회지
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• 제24권5호
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• pp.779-784
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• 1995

5'-GMP(5'-Guanylic acid) 생산 융합균조 fusant RC102를 고정화하여 5'-GMP을 연속적으로 생산하고자 하였다. 균체 고정화용 담체로서 k-carrageenan, polyacrylamide, Ca-alginate 및 agar 등 4가지 담체에 고정화한 결과 최적 담체로서 3% k-carrageenan을 선정하였으며, 이담체에 고정화한 균체를 이용하여 5'-GMP 연속적으로 생산하였다. 고정화 균체에 의한 5'-GMP 생산의 최적 온도 및 pH은 $32^{\circ}C$, pH 8.0, 미량온소로서 $Mn^{2+}과\;Zn^{2+}$의 농도는 각각 $30\mu\textrm{g}/L,\;1{\times}10^{-6}%$이었다. $Mn^{2+}$이 풍부하고 값싼 CSL(Corn Steep Liquor)을 배지로서 사용하기 위해서 penicillin G와 D-cycloserine 및 POSEA(polyoxyethylene stearylamine) 농도 최적 농도는 각각 0.8unit/ml, 0.8unit/ml, 그리고 5mg/ml으로 나타났으며, 항생물질 보다 계면활성제가 효과적이었다. 이상의 최적 조건에서 고정화 fusant RC102는 15일간 안정되게 5'-GMP를 생산하였다.

• 약학회지
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• 제42권6호
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• pp.621-626
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• 1998

Cactus (Opuntia ficus-indica var saboten Makino) is a tropical or subtropical plant, which is widely used as folk medicine for burned wound, edema and indigestion. We previously found that the ethanol extract of cactus stem showed anti-inflanunatory action. This investigation was designed to isolate the active fraction of anti-inflanimatory action from cactus stem by solvent extraction and colunm chromatography. Carrageenan-induced paw edema in rats and acetic acid-induced writhing test in mice were used as animal models to search anti-inflammatory and analgesic activities. respectively. The ethanol extract of cactus stem was consecutively extracted with hexane, ethyl acetate, and n-butanol. The hexane fraction was the most effective in carrageenan-induced paw edema, and then was separated in colunm chromatography of silica gel by the elution with hexane/ethyl acetate mixture. The most effective fraction 1 was separated in a second colunm chromatography by eluting with hexane/diethyl ether mixture. The most effective fraction 1-5 was obtained, and separated in a third column chromatography by eluting with hexane/chloroform mixture. It produced the most effective fraction 1-5-1. Moreover, fraction 1-5-1 showed an inhibitory effect on acetic acid-induced writhing in the doses of 30mg/kg and 60mg/kg,p.o.,indicating that it also contained analgesic activity.

• Korean Journal of Acupuncture
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• 제23권1호
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• pp.67-85
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• 2006

Obiecnves : The purpose of this study was to examine the analgesic effect of moxi-tar on a rat model of carrageenan-induced arthritis and the relations between moxi-tar-induced analgesia and endogenous NO and iNOS, cyclooxygenase-2 (COX-2), and c-Fos protein expression in the spinal cord. Methods : Carrageenan-induced arthritis rat model was used to test the effect of moxi-tar as a chronic pain model. After the induction of arthritis, rats subsequently showed a reduced stepping force of the affected limb for at least the next 4 days. The reduced stepping force of the limb was presumably due to a painful knee, since oral injection of indomethacin produced temporary improvement of weight bearing. Moxi-tar dissolved in ethyl alcohol was injected several acupoints. After the treatment, behavioral tests measuring stepping force were periodically conducted during the next 12 hours. Results : Moxi-tar produced significant improvement of stepping force of the hindlimb affected by the arthritis lasting at least 9 hours. The magnitude of this improvement was equivalent to that obtained after an oral injection of 3 mg/kg of indomethacin and this improvement of stepping force was interpreted as an analgesic effect. Moxi-tar produced the improvement of stepping force of the affected hindlimb in a dose-dependent manner. Both NO production and iNOS, COX-2 protein expression increased by arthritis were suppressed by moxi-tar. moxi-tar on combination with electroacupuncture (EA) produced more powerful and longer lasting improvement of stepping force of the hindlimb affected by the arthritis than either moxi-tar or EA did. Conclusion : The present study suggest that moxi-tar produces a potent analgesic effect on the chronic knee arthritis pain model in the rat and that moxi-tar-induced analgesia modulate endogenous NO through the suppression of iNOS/COX-2 protein expression.

• 한국전통의학지
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• 제15권1호
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• pp.113-127
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• 2006

Objectives: This study was produced to examine the effects of moxibustion that had been played important role to traditional oriental medical treatment on disease. Recently, it was reported that moxi-tar which is generated in the process of moxibustion as burning combustibles decreased nitric oxide(NO) and inducible NO synthase (iNOS) generation in cellular experiments. Methods: Carrageenan-induced arthritis rat model was used to test the effect of moxi-tar as a chronic pain model. Diluted moxi-tar was single injected in several acupoints or combined with electroacupuncture (l ms, 2 Hz, and 2 mA) into contralateral ST36 acupoint for 30 min to assess the synergic effects. After the treatment, behavioral tests measuring stepping force were periodically conducted during the next 12 hours. Endogenous NO and iNOS, cyclooxygenase-2 (COX-2), and c-Fos protein expression in the spinal cord were examined on a rat model of carrageenan-induced arthritis. Results : After the induction of arthritis, rats subsequently showed a reduced stepping force of the affected limb for at least the next 4 days. The reduced stepping force of the limb was presumably due to a painful knee, since oral injection of indomethacin produced temporary improvement of weight bearing. Maxi-tar produced significant improvement of stepping force of the hindlimb affected by the arthritis lasting at least 9 hours. The magnitude of this improvement was equivalent to that obtained after an oral injection of 3 mg/kg of indomethacin and this improvement of stepping force was interpreted as an analgesic effect. Maxi-tar produced the improvement of stepping force of the affected hindlimb in a dose-dependent manner. Both NO production and iNOS, COX-2 protein expression increased by arthritis were suppressed by maxi-tar. Moxi-tar on combination with electroacupuncture (EA) produced more powerful and longer lasting improvement of stepping force of the hindlimb affected by the arthritis than either moxi-tar or EA did. Conclusion : The present study suggest that maxi-tar produces a potent analgesic effect on the chronic knee arthritis pain model in the rat and that moxi-tar-induced analgesia modulate endogenous NO through the suppression of iNOS/COX-2 protein expression.

• 한국식품영양학회지
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• 제26권3호
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• pp.428-437
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• 2013

The physical properties of jellified soybean curd residue (Biji) with the addition of various gums were investigated to optimize the direct availability of a by-product of the tofu production process. The lightness (L value) of the jellified Biji prepared with various gums was approximately 77~80, regardless of the concentration of gum used. Especially at the concentration of 0.1% and 0.3%, a pectin showed a significantly higher value compared with other gelling agents (carrageenan and agar). The strength of samples prepared on the basis of carrageenan showed the value of 335~569 $g/cm^2$ and 234~335 $g/cm^2$ according to the addition of locust bean gum and xanthan gum, respectively. On the other hand, the samples made with pectin and agar were 134~272 $g/cm^2$ and 50~215 $g/cm^2$, respectively. Where locust bean gum was used as a viscous agent, the adhesiveness of sample prepared by adding carrageenan was greater than those made with pectin or agar. The values were -6.0~-11.0 g, -2.7~-10.2 g and -3.7~-7.0 g, respectively. Overall, the strength and adhesiveness of samples according to increase of the added amount of Biji showed constantly increasing trend regardless of the type of gelling agent used. In the sample containing carrageenan this trend was most noticeable.