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Mass Cultivation and Secondary Metabolite Analysis of Rhodobacter capsulatus PS-2 (광합성세균 Rhodobater capsulatus PS-2의 대량배양 최적화 및 대사산물 분석)

  • Bong, Ki Moon;Kim, Jong Min;Yoo, Jae-Hong;Park, In Chul;Lee, Chul Won;Kim, Pyoung Il
    • KSBB Journal
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    • v.31 no.3
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    • pp.158-164
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    • 2016
  • Plant growth promoting (PGP) hormones, which are produced in a small quantity by bacteria, affect in plant growth and development. PGPs play an important role on the crop productivity in agricultural field. In this study, a photosynthetic bacterial strain producing the PGP was isolated from paddy soil. Bacterial isolate was gram negative, rod-shaped and motility positive. From the 16s rRNA gene sequence analysis, the isolate was identified as Rhodobacter capsulatus PS-2. The mass cultivation of R. capsulatus PS-2 was optimized by considering of the carbon, nitrogen and inorganic salt sources. Optimal medium composition was determined as Na-succinate 4.5 g, yeast extract 5 g, $K_2HPO_4$ 1 g, $MgSO_4$ 5 g, per liter. From the result of 500 L fermentation for 2 days using the optimal medium, the viable cells were $8.7{\times}10^9cfu/mL$. R. capsulatus PS-2 strain produced the carotenoid and indole-3-acetic acid (IAA). The carotenoid extraction and quantitative analysis were performed by HCl-assisting method. Total carotenoid contents from R. capsulatus PS-2 culture broth were measured as $7.02{\pm}0.04$ and $6.93{\pm}0.05mg/L$ under photoheterotrophic and chemoheterotrophic conditions, respectively. To measure the productivity of IAA, colorimetric method was employed using Salkowski reagent at optical density 535 nm. The results showed that the highest content of IAA was $197.44{\pm}5.92mg/L$ in the optimal medium supplemented with 0.3% tryptophan.

Factor Analysis of the Seawater Quality of the Southern Coastal Waters of Korea

  • Lee Yong-Hwan;Jung Kyoo-Jin;Kim Hak-Kook
    • Fisheries and Aquatic Sciences
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    • v.6 no.3
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    • pp.140-148
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    • 2003
  • On the basis of factor analysis, stations were grouped according to their similar characteristics of seawater quality. The data for factor analysis were collected from the 15 stations from Dukryang Bay to Ulsan Bay on the southern cost of Korea. The study was based on the data from 1991 to 2000. The 8 water quality items analyzed were temperature, salinity, pH, DO, COD, DIN (dissolved inorganic nitrogen), DIP (dissolved inorganic phosphorus), and SS (suspended solid). Analysis of 6 water quality items including DO with the exception of temperature and salinity showed that 15 stations were grouped into two zones, i.e., the western and the eastern coast, by the axis of Samcheonpo-Jinju Bay-south of Geoje, 3 seawater zones in all. The adjacent stations to the southward or northward but not those to the eastward or westward were classified into the same group. On the analysis of all of the 8 water quality items, the stations of Dukryang Bay and Goheung; and those of Onsan and Ulsan Bay were classified into the same group. Yeosu and Namhae stations were sectioned into 1 group on the all seawater quality items but DIP, Samcheonpo and south of Geoje stations another group on all seawater quality items but water temperature, and Masan and Busan stations in the other group on all seawater quality items but DO. The stations from Dukryang Bay through Goheung to east of Geoje were grouped together on the COD item, and this showed somewhat different tendency in other seawater quality items.

Qualitative Development of Herbicide Use in Crop Production -On the Compatibility of Herbicide Use in Soybean Production (농약(제초제)의 품질개발에 관한 연구 -대두 제초제의 사용 적합성을 중심으로)

  • Guh, J.O.;Kim, Y.J.;Choi, W.Y.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.24 no.2
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    • pp.89-103
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    • 1979
  • The compatibility of herbicide use for soybean cultivation was evaluated from the field and pot trials. Comparing with the check plot, the effects of herbicides at their several doses on the phytotoxic stability of crop plants and on the weeding efficacy were studied. Statistically significant differences were found among the doses, and not among herbicides and interactions between herbicide and dose. Risks on the compatibility for crop plant and weeding efficacy were recognized from the excess-dosed treatments of herbicides tried.

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The Linkage of Health Care and Social Services for the Elderly in Jeonnam Province and its Related Factors (전라남도 노인보건복지에 있어서 방문간호사와 사회복지전담공무원의 서비스 연계실태 및 관련요인)

  • Rhee, Jung-Ae;Chung, Eun-Kyung;Ham, Cheol-Ho;Lee, Young-Hun
    • Health Policy and Management
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    • v.16 no.1
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    • pp.41-53
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    • 2006
  • This study was carried out to investigate the linkage between health care and social services for the elderly in Jeonnam Province and its related factors. The subjects were 831 persons of visiting health service workers and social welfare workers in Jeonnam Province who responded the mail questionnaire (85.5% respondse rate). The data were collected from 15th May to 30th June, 2004 using the structured questionnaire. It was composed of the socio-demographic characteristics, the content and frequency of services referred between health care and social welfare workers, and interdisciplinary perception. Data was analysed by SPSS for Windows 12.0. 374(45.0%) reported having experience of service cooperation between health care and social welfare workers for the last three month. The most service that health care workers requested to social welfare workers was to get information for the client, on the other hand the most service that social welfare workers requested to health care workers was to visit and treat the elderly. These service providers in rural area and good attitude toward the cooperation between health care and social welfare service showed more linkage. The findings of this study could provide the basic data for the development of efficient coordinating program of health and welfare.

Effect of Packing Methods on Green Powder Tea Quality during storage at Cold Temperature (가루차 저온 저장 중 포장방법별 품질변화)

  • Park Jang-Hyun;Bac Chang-Nam;Kim Yong-Ok;Choi Hyeong-Kuk
    • Food Science and Preservation
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    • v.12 no.1
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    • pp.1-7
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    • 2005
  • The effect of packing methods on the green powder tea were investigated by examining quality changes during cold stroage. Packing was performed with aluminum packs having an antioxidant and a wateiproofing agent in vacuum Alumiunum packing with treatment or not showed little changes till 2 months but after this period remarkable quality degradation were found in total nitrogen, total amino acids, tannin, caffeine, chlorophyll and fatty acids. The color changes of gieeness were -16.43 in one month and -10.11 in five months, respectively. Alumininum packing in vacuum was showed extention of storage period above one month.

Studies on Status and hematological Values of Reproductive Disorders in Dairy Cattle of Jeonnam Area (전남지방(全南地方) 유우(乳牛)에 있어서 번식장해(繁殖障害)의 실태(實態) 및 그 혈액치(血液値)에 관(關)한 조사연구(調査硏究))

  • Park, Young Jun
    • Korean Journal of Veterinary Research
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    • v.14 no.2
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    • pp.253-268
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    • 1974
  • A total of 331 dairy cattle in Jeonnam area were examined for the breeding status and hematological values during the period from June to August. 1971 and 1974. The data obtained were analysed according to the status of breeding and the type of farm management. The results obtained in this work were summarized as follows: 1. 331 dairy cows were grouped as pregnant (63.81%) anestrus after parturition (12.45%), pregnancy unknown (11.48%), repeat breeder (10.32%), and others (1.94%). 2. The summery of reproductive histories and clinical examination were as follows. Average of calving interval was 16.5 months, interval from parturition to first breeding 97 days and postpartum interval to first estrus 72 days. Services per conception was 1.6 rate of postpartum estrus (60 days) 12.0%, and the rate of repent breeder 10.3%. 3. Generally, the blood values of RBC, Hb, serum total protein and A/G ratio were lower than those normal values, especially, the cows which showed abnormal values belonged to the repeat breeder and the unknown to conception group. The mean value for serum inorganic phosphorus was the normal value or hyperphosphatemia, on the other hand, the mean value for serum calcium of the repeat breeder group was the lowest than the other group. 4. Follow-up evaluations on the results of the laboratory tests strongly suggest that the problems of repeat breeder had a tendency to occur more frequently in the large herd (A and B type farm), and the Ca/P ratio of almost all the cows showed abnormal values.

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Chemical Composition and Physiological Activity of Native Pear c.v. ‘Baekwoon’ (백운배의 화학성분과 생리활성 효과)

  • Heo, Buk-Gu;Park, Yong-Seo;Park, Yun-Jum;Jung, Kyoo-Jin;Cho, Ja-Yong;Oh, Kyung-Taek;Chung, Ung-Seo;Lee, Kyung-Dong
    • The Korean Journal of Community Living Science
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    • v.20 no.4
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    • pp.549-558
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    • 2009
  • This study examined the basic information on the native pear c.v. 'Baekwoon' grown in Mt. Baekwoonsan, Gwangyang, to develop as a local specialty product. the sensory characteristics, chemical compositions, and physiological activities of 'Baekwoon' pear were examined and compared with those of 'Niitaka' pear. Hardness of 'Baekwoon' pear was 19.30N, which was higher than 'Niitaka'. The sugar content of 'Baekwoon' was 11.5 $^{\circ}Brix$, which was lower than 'Niitaka'. The total amino acid contents of the fruit skin and flesh of 'Baekwoon' were 222 mg/100g dry weight (DW) and 130 mg/100g DW, respectively, which were much higher than those of 'Niitaka'. Especially, serine contents in fruit skin and flesh of 'Baekwoon' were 75.4 mg/100g DW and 40.2 mg/100g DW, respectively, which were significantly (p<0.05) higher than those of 'Niitaka'. However, physiological activities, such as total phenol content, DPPH radical scavenging activity, total flavonoid content and nitrite scavenging activity, of 'Baekwoon' were lower than or similar to those of 'Niitaka'. Consequently, vitamin and amino acid contents of 'Baekwoon' were better than those of 'Niitaka'. It is suggested to use fruit skin because of its good composition and physiological activities so that it helps to improve the quality and the biological activity.

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Sporulation and Dissemination of Pycnidiospores of Diaporthe citri in Yuzu Tree (Citrus junos Sieb) in Jeonnam Area (전남지역 유자과원의 검은점무늬병균 포자 형성과 비산)

  • Hur, Kil-Hyun;Park, Seur-Kee
    • Research in Plant Disease
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    • v.11 no.1
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    • pp.16-20
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    • 2005
  • Several time-course experiments were carried out to understand seasonal development of melanose on yuzu trees at koehung area, Jeonnam province, during May to October. The occurrence of dead twigs, known as a source of infection, was much more in older trees, and from June to August, mostly in July. In the experiment of pycnidia development on dead twigs seasonally collected, the number of developed pycnidia was highest on July-collected dead twigs especially with the diameter of 1.1~1.5 cm. In the collection survey of disseminated pycnidiospores, although the collected number of pycnidiospores was affected with amount of precipitation, the number of observed pycnidiospores in rainwater was relatively high from June to August, with highest in early August in 1997 and late July in 1998. In the inoculation tests on 3-year-old trees and fruits in natural condition, disease occurrences were mostly affected on twigs by inocula treatment in June, and on fruits by inocula treatment in July, respectively.

Aspect of Occurrence of Melanose (Diaporthe citri Wolf) on Yuzu Trees (Citrus junos Sieb) in Jeonnam Area, in Korea (전남지역 유과자원에서의 검은점무늬병 발생 양상)

  • Hur, Kil-Hyun;Park, Seur-Kee
    • Research in Plant Disease
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    • v.11 no.1
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    • pp.10-15
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    • 2005
  • The occurrence of melanose was investigated on yuzu at Goheung and Boseong in Jeonnam province in 1997 and 1998 seasons. The disease occurrence on leaf was initiated from late May in 1997 and early May in 1998, severest in late of June on leaf, and in case of fruit, initiated from early July in 1997 and late June in 1998 with a peak in early August. The disease was occurred in twenty days more earlier in 1998 season than 1997 season. We think this was caused by temperatures during April and May. And it was supposed that the disease severity on the severest late of June was closely related to the rainfall and relative humidity during June. The disease severity was severer in old tree than the young.

A Study on the Refresher Training of Physical Therapist in Gwangju and Jeonnam

  • Yu, Seong-Hun;Kim, Seung-Rae;Cho, Sung-Hyoun;Jang, Il-Yong;Hwang, Jin-Ah;Kim, Yong-Seong;Kim, Hyun-Jin
    • The Journal of Korean Physical Therapy
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    • v.28 no.3
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    • pp.165-175
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    • 2016
  • Purpose: The current study seeks to examine the continuing education of physical therapist in Gwangju, Jeonnam. Methods: A survey was conducted during the continuing education of physical therapist held in Gwangju in 2015 with 297 participants. The survey questionnaire consisted of 13 questions on characteristics of physical therapist, 7 questions on the level of satisfaction with continuing education, 11 questions regarding the need for continuing education, and 8 questions concerning how to improve continuing education. Results: As for the level of satisfaction with the content of continuing education, the survey results indicated that there were significant differences across respondents' age and career period. Regarding the level of satisfaction with the environment in which continuing education was provided; significant differences were observed across respondents' age, education, marital status, monthly pay, career period, and service period. Regarding the level of satisfaction with the expense in which continuing education was provided; significant differences were observed across respondents' sex, age, education, marital status, dependent family, monthly pay, career period, and position. In terms of the level of satisfaction with the operation method in which continuing education was carried out, there were significant differences across respondents' age, education, and career period. Conclusion: The survey found the level of satisfaction with continuing education to be average among physical therapist in Gwangju and Jeonnam. In addition, as for ways to take continuing education courses for those who have never taken it, online education was mentioned the most. Therefore, there is a need for advertising online continuing education.