• Title/Summary/Keyword: jasmonate

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Elicitors for the Regulation of baccatin III Biosynthesis in Plant Cell Culture System (Elicitor에 의한 baccatin III의 세포내 생합성 조절연구)

  • Shin, Seung-Won;Kim, You-Sun;Lim, Sook
    • YAKHAK HOEJI
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    • v.44 no.1
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    • pp.60-65
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    • 2000
  • The yeast extract, coconut water, safflower seed oil, arachidonic acid, linolenic acid, jasmonic acid and methyl jasmonate were added to Gamborg's B$_{5}$ medium. The changes on productivity of baccatin III were estimated every 30 minutes and the results were compared using the selected high yielding cell culture system of Taxus cuspidata. In most cases, the peaks of baccatin III productivity occured at 90~120 min after addition of elicitors. Among the compound elicitors, safflower seed oil showed the highest productivity of baccatin III. Also arachidonic acid and linolenic acid increased the baccatin III production.

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Induction of methylnissolin in the adventitious root of Astragalus by methyl jasmonate

  • Kim, Jong-Kwun;Im, Seung-Yun;Lee, Hyun-Jin;Sung, Chung-Ki
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.266.3-267
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    • 2003
  • In order to induce production of methylnissolin, sterol biosyntheis inhibitor, in the adventitious root of Astragalus membranaceus the effect of methyl jasmonate(MeJ), a growth regulator of plant, was investigated. After treatment of MeJ (0$\mu\textrm{m}$, 10$\mu\textrm{m}$, 100$\mu\textrm{m}$) to the adventitious root which was harvested in the time interval of 0, 7, 14, 21, 28days and the fresh weight, dry weight and the contents methylnissolin was determined. (omitted)

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Elicitor-induced accumulation of stilbenes in cell suspension cultures of Cayratia trifolia (L.) Domin

  • Roat, Chetana;Ramawat, K.G.
    • Plant Biotechnology Reports
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    • v.3 no.2
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    • pp.135-138
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    • 2009
  • Cell cultures of Cayratia trifolia (Vitaceae), a tropical lianas, were maintained in Murashige and Skoog's medium containing $0.25mg\;1^{-1}$ NAA, $0.2mg\;1^{-1}$ kinetin and casein hydrolysate $250mg\;1^{-1}$. Cell suspension cultures of C. trifolia accumulate stilbenes (piceid, resveratrol, viniferin, ampelopsin), which on elicitation by any of $500{\mu}M$ salicylic acid, $100{\mu}M$ methyl jasmonate, $500{\mu}M$ ethrel and $500mg\;1^{-1}$ yeast extract, added on the 7th day, were enhanced by 3- to 6-fold ($5-11mg\;1^{-1}$) by the 15th day.

Effect of Methyl Jasmonate on in vitro Bulblet Formation and Enlargement from Shoot Clump of Allium victorialis (산마늘 다신초 덩어리로부터 인경 형성과 비대에 미치는 methy jjasmonate의 영향)

  • Park, So-Young;Ahn, Jin-Kwon;Lee, Wi-Young;Park, Hae-Chin
    • Journal of Plant Biotechnology
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    • v.31 no.1
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    • pp.79-82
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    • 2004
  • The effect of abscisic acid (ABA) and methyl jasmonate (MeJA) on bulblet formation from the culture of Allium victorialis var. platyphyllum Makino was studied. Shoot clumps were cultured on MS medium containing ABA (0, 0.01∼2.0mg/L) and MeJA (0.01∼5.0mg/L). ABA at low concentrations (0.01mg/L) induced shoot proliferation without bulblet formation. However, bulblet formation started on the medium containing MeJA approximately in 4-6 weeks of culture. Furthermore, 1.0mg/L MeJA resulted in bulblet formation at high frequency (100%) as compare to the control (46.1%). Cortical cells of the bulblets enlarged on medium with MeJA had dense protein-like substance in expanded and round cells when examined under the microscope. The data described here show that formation and enlargement of bulblets from Allium victorialis can be improved by addition of appropriate concentration of MeJA.

Effects of Light and Some Plant Growth Regulators on Ecdysteroids Contents in Polypodium vulgare L. and Achyranthes japonica Nakai (식물의 Ecdysteroids 함량에 미치는 광과 식물생장조절제의 영향)

  • Chae, Hyun-Byung;Boo, Kyung-Hwan;Jin, Seong-Beom;Lee, Do-Seung;Kim, Dae-Woon;Cho, Moon-Jae;Riu, Key-Zung
    • Applied Biological Chemistry
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    • v.44 no.3
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    • pp.162-166
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    • 2001
  • Effects of light, methyl jasmonate(MJ), 6-benzylaminopurine(BA), thidiazouron(TDZ), and 2,4-dichlorophenoxyacetic acid(2,4-D) on the contents of ecdysteroids (${\beta}$-ecdysone+polypodine B) in Polypodium vulgare L. and Achyranthes japonica Nakai were studied. When the plants of P. vulgare were cultured under light control, the ecdysteroids contents in both leaves and rhizomes decreased with increasing light intensity. The ecdysteroids contents in A. japonica were also lower when cultured under light than under dark. Among the tested plant growth regulators, MJ, BA, and TDZ increased the ecdysteroids contents in both P. vulgare and A. japonica.

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Physico-chemical properties of green leaf volatiles (GLV) for ascertaining atmospheric fate and transport in fog

  • Vempati, Harsha;Vaitilingom, Mickael;Zhang, Zenghui;Liyana-Arachchi, Thilanga P.;Stevens, Christopher S.;Hung, Francisco R.;Valsaraj, Kalliat T.
    • Advances in environmental research
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    • v.7 no.2
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    • pp.139-159
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    • 2018
  • Green Leaf Volatiles (GLVs) is a class of biogenically emitted oxygenated hydrocarbons that have been identified as a potential source of Secondary Organic Aerosols (SOA) via aqueous oxidation. The physico-chemical properties of GLVs are vital to understanding their fate and transport in the atmosphere via fog processing, but few experimental data are available. We studied the aqueous solubility, 1-octanol/water partition coefficient, and Henry's law constant ($K_H$) of five GLVs at $25^{\circ}C$: methyl jasmonate, methyl salicylate, 2-methyl-3-buten-2-ol, cis-3-hexen-1-ol, and cis-3-hexenyl acetate. Henry's law constant was also measured at temperatures and ionic strengths typical of fog. Experimental values are compared to scarcely-available literature values, as well as estimations using group and bond contribution methods, property-specific correlations and molecular dynamics simulations. From these values, the partition coefficients to the air-water interface were also calculated. The large Henry's law constant of methyl jasmonate ($8091{\pm}1121M{\cdot}atm^{-1}$) made it the most significant GLV for aqueous phase photochemistry. The HENRYWIN program's bond contribution method from the Estimation Programs Interface Suite (EPI Suite) produced the best estimate of the Henry's constant for GLVs. Estimations of 1-octanol/water partition coefficient and solubility are best when correlating an experimental value of one to find the other. Finally, the scavenging efficiency was calculated for each GLV indicating aqueous phase processing will be most important for methyl jasmonate.