• Journal of The Korean Society of Grassland and Forage Science
• /
• v.36 no.4
• /
• pp.333-339
• /
• 2016

The objective of this study was to optimize analytical methods for ochratoxin A (OTA) and zearalenone (ZEA) in rice straw silage and winter forage crops using ultra-high performance liquid chromatography (UHPLC). Samples free of mycotoxins were spiked with $50{\mu}g/kg$, $250{\mu}g/kg$, or $500{\mu}g/kg$ of OTA and $300{\mu}g/kg$, $1500{\mu}g/kg$, or $3000{\mu}g/kg$ of ZEA. OTA and ZEA were extracted by acetonitrile and cleaned-up using an immunoaffinity column. They were then subjected to analysis with UHPLC equipped with a fluorescence detector. The correlation coefficients of calibration curves showed high linearity ($R^2{\geq_-}0.9999$ for OTA and $R^2{\geq_-}0.9995$ for ZEA). The limit of detection and quantification were $0.1{\mu}g/kg$ and $0.3{\mu}g/kg$, respectively, for OTA and $5{\mu}g/kg$ and $16.7{\mu}g/kg$, respectively, for ZEA. The recovery and relative standard deviation (RSD) of OTA were as follows: rice straw = 84.23~95.33%, 2.59~4.77%; Italian ryegrass = 79.02~95%, 0.86~5.83%; barley = 74.93~97%, 0.85~9.19%; rye = 77.99~96.67%, 0.33~6.26%. The recovery and RSD of ZEA were: rice straw = 109.6~114.22%, 0.67~7.15%; Italian ryegrass = 98.01~109.44%, 1.65~4.81%; barley = 98~113.53%, 0.25~5.85%; rye = 90.44~108.56%, 2.5~4.66%. They both satisfied the standards of European Commission criteria (EC 401-2006) for quantitative analysis. These results showed that the optimized methods could be used for mycotoxin analysis of forages.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.30 no.2
• /
• pp.115-120
• /
• 2010

This study was carried out to determine the forage production and quality of Italian ryegrass (IRG) and forage barley sown in early spring in Suwon, 2009. The five treatments used in this experiment were IRG Kowinearly (early maturity), IRG Kowinmaster (medium maturity), Yuyeon barley, Kowinearly + Yuyeon mixture, and Kowinmaster + Yuyeon mixture. The lodging was observed in IRG, but no lodging was found IRG + barley mixtures. The heading date of Kowinearly and Kowinmaster were 16 May and 22 May, respectively, and that of barley was 13 May. The dry matter (DM) percentage at harvest was 22.2~27.6%. The forage quality among treatments were similar, but the crude protein (CP) content of IRG was higher than that of barley, and in vitro DM digestibility was a little low in Kowinmaster. The yields of DM, CP and digestible DM were high in Kowinearly + Yuyeon barley mixtures as a 13,816 kg, 1,384 kg and 10,387 kg per ha, respectively (p<0.05). In conclusion, the mixture cultivation of IRG and forage barley was very effective, because of preventing of IRG lodging, increasing of forage yield, and stable production of forages. The optimum harvest date for silage manufacture of IRG and barley sown in early spring was recommended early June instead of May.

• Korean Journal of Weed Science
• /
• v.11 no.1
• /
• pp.50-59
• /
• 1991

Residual period and carry-over effect of some herbicides were determined using a bioassay method in six summer crops(potato, carrot, corn, water melon, soybean, and sesame). The effects were measured at regular time intervals after applying different rates of the herbicides. There were no great differences in residual period and carry-over injury between the soils and kinds of crops used. However, the residual period varied with the herbicides studied and the carry-over injury was dependent upon season and rate of the herbicide application, sampling depth of soil, and kind and seeding date of the test plant. When the residual herbicides were applied, the carry-over injury could be minimized by selecting tolernet crops, delaying seeding of the crops after application of the herbicides, and regulating the cultivation depth. Herbicides which showed no residual effect by the end of the cropping period(100-120 days for summer crops) and no carry-over effect were alachlor, trifluralin, ethalfluralin, metribuzin, and prometryn. When pendimethalin, metolachlor, linuron, methabenzthiazuron, and simazine were applied at the recommended rate or less, there was no carry -over injury at harvesting time. With doubling the recommended rate, however, the carry-over effect was found in sensitive crops. Napropamide applied at the rate of 300 g a.i./10 a brought about carry-over injury for Italian ryegrass and barley at 140 days in summer crops, whereas the injury was not found in Cruciferae (radish, Chinese cabbage). Nitralin applied at the rate of 150-300 g a.i./10a caused the carry-over injury for Italian ryegrass and barley at 140 days in summer crops. However, there was no injury for Cruciferae.

• Korean Journal of Weed Science
• /
• v.11 no.1
• /
• pp.32-49
• /
• 1991

Residual period and carry-over effect of some herbicides were determined using a bioassay method in five winter crops (chinese cabbage, radish, spinach, onion and garlic). The effects were measured at regular time intervals after applling different rates of the herbicides. There were no great differences in residual period and carry-over injury between the soils and kinds of crops used. However, the residual period varied with the herbicides studied and the carry-over injury was rate of the herbicide application, sampling depth of soil, and kind and seeding date of the test plant. When the residual herbicides were applied, the carry-over injury could be minimized by selecting tolerant crops, delaying seeding of the crops after application of the herbicides, and regulating the cultivation depth. Herbicides which showed no residual effect by the end of the cropping period (200-240 days for winter crops) and no carry-over effect were alachlor, trifluralin, ethalfluralin and prometryn. When pendimethalin, metolachlor, linuron and methabenthiazuron were applied at the recommended rate or less, there was no carry-over injury at harvesting time. With doubling the recommended rate, however, the carry-over effect was found in sensitive crops. Napropamide applied in winter crops at rate of 150-300g a.i./10a brought about carry-over injury for such Gramineae as Italian ryegrass, direct-seeded rice and barley, whereas the injury was not found in lowland-transplanted rice, Cruciferae, Cucurbitaceae and Solanaceae. Long residual herbicide nitralin applied at the rate of 75g a.i./10a caused the carry-over injury for Italian ryegrass, direct-seeded rice, baley and lowland-transplanted rice at 275 days in winter crops. In addition, a slight injury occurred in sesame, perilla and spinach, However, there was no injury for Cruciferae, Cucurbitaceae and Solanaceae.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.27 no.1
• /
• pp.29-36
• /
• 2007

A study was conducted to determine the trace minerals (Cu, Fe, Cd, Zn, Mo, Se, Mn, Cr, Co, Ni) levels in pasture soils and forages collected in Jeju area. Chemical characteristics and total or soluble trace minerals levels in 187 pasture soil samples (76 very dark brown and 111 black soils) were measured. Total trace minerals contents in hay samples of 60 Italian ryegrass, alfalfa and mixture grasses each were assessed. The pasture soils of Jeju were characterized to have low pH (5.1) and to contain low levels of available $P_2O_5$ (20.5 mg/kg) and exchangeable Ca, Mg, K, Na (2.6, 0.9, 0.5, 0.2 cmol+/kg, respectively) when comparing to upland soils of Jeju or Korean mainland. All trace elements in total or soluble analysis of pasture soils were detected, and there was a big difference between total and soluble levels. The pasture soils tended to have the higher total Fe, Mn, Ni and Zn contents. Cr in all forages was not detected, but other trace minerals levels showed normal range. The hay samples of Italian ryegrass and mixture grasses produced in Jeju tended to contain higher Mn (105 vs 23 mg/kg) and lower Mo (2.7 vs 4.9 mg/kg) than those of alfalfa hay imported from USA. Results show that trace minerals of pasture soils and forages in Jeju seem to be not deficient, indicating that supplementation of some trace minerals are not always necessary in diets for grazing animals and should be done after careful evaluation of diets with regard to concentrations and biological availability of essential elements.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.31 no.1
• /
• pp.39-46
• /
• 2011

This study was carried out to determine the forage yield, quality, and regrowth of Italian ryegrass (IRG) and barley sown on 2 March 2009 in Suwon. The five treatments were two IRG cultivars (Kowinearly with early maturity and Kowinmaster with medium maturity), one barley cultivar (Yuyeon), and two mixtures (Kowinearly + Yuyeon and Kowinmaster + Yuyeon). The first harvest date was 26 May, which was at late heading, heading and early dough stage of Kowinearly, Kowinmaster and barley, respectively. Regrowth yield was investigated on 29 June. The heading dates of Kowinearly and Kowinmaster were 16 May and 22 May, respectively, and that of barley was 13 May. The dry matter (DM) percentage were 13.0~18.4% at first harvest, and 22.5~24.8% at regrowth in all treatments. The forage yield of barley and Kowinmaster + Yuyeon mixture at first harvest was higher than that of IRG (p<0.05), but higher regrowth yield was observed in IRG, and then IRG + barley mixtures (p<0.05). The crude protein (CP) content and in vitro DM digestibility (IVDMD) of IRG at first harvest were 16.7~17.1% and 78.3~80.4%, respectively, which were higher than those of barley (CP 12.2% and IVDMD 72.6%) and IRG + barley mixtures. The total yields of DM, CP and digestible DM were high in Kowinmaster + Yuyeon mixture as 11,628 kg, 1,669 kg and 8,457 kg per ha, respectively. In conclusion, spring seeding of IRG + barley mixtures and/or barley were recommended when early harvest. Regrowth of IRG sown in early spring was vigorous. Mixture cultivation of IRG and barley was effective, because of forage yield and stable production, and harvest at June instead of May was desirable for forage productivity of spring sown IRG and barley.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.27 no.2
• /
• pp.85-92
• /
• 2007

This study was carried out to select the promising forage crops fer forage production in paddy field of YARI, RDA, Milyang from 1999 to 2001. The species of forage crop used in this experiment were barley(5 varieties), wheat(2), rye(3) and Italian ryegrass(IRG, 3). Stages of heading, milk and yellow ripe of barley were $20{\sim}24$ April, $6{\sim}10$ May and $21{\sim}24$ May, respectively. In milk to yellow ripe stage, dry matter(DM) yield of barley was $7.89{\sim}9.66$ MT per ha, and averaged ADF and NDF contents were $26.9{\sim}33.3%\;and\;53.1{\sim}59.2%$, respectively. Albori and Naehanssalbori were the most promising varieties among the barley. The growth of wheat was a little late compared to that of barley, but it seemed to be crop having higher forage yield and nutritive value. In milk to yellow stage, DM yield of wheat was $9.13{\sim}10.38$ MT per ha, and nutritive value of wheat was very good(ADF $25.9{\sim}31.7%$ and NDF $53.3{\sim}55.8%$). Heading stage of rye was $20{\sim}24$ April, and it seemed to be suitable far harvest at heading to flowering stage because of heavy lodging after flowering. In flowering stage, DM yield of rye was 13.64 MT per ha, and nutritive value of rye was the lowest among 4 species(ADF $33.1{\sim}38.0%$ and NDF $56.4{\sim}65.0%$). Heading stage of early maturing IRG was 2 May and it seemed to be suitable for harvest at flowering stage of middle May in cropping system. In flowering stage, DM yield of early type IRG was 5.51 MT per ha, and averaged ADF and NDF contents were $30.1{\sim}34.7%\;and\;59.7{\sim}60.5%$, respectively. The results demonstrated that the promising forage crops far forage production in paddy field were rye, barley, early maturing IRG and wheat in Yeongnam region. In Southern region, rye harvesting at early May was recommended when considered middle May of rice transplanting period, and barley(Albori and Naehanssalbori) and early maturing IRG harvesting at middle May were recommended when considered late May of rice planting period. And also, wheat with high nutritive value and possibility for harvesting at late May were recommended in case of transplanting period at early June.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.24 no.3
• /
• pp.207-216
• /
• 2004

This study was carried out to select the promising forage crops for forage production in paddy field of NLRI, RDA, Suwon from 1999 to 2001. The species of forage crop used in this experiment were barley(3 varieties), wheat(2), rye(3) and Italian ryegrass(IRG, 3). Stages of heading, milk and yellow ripe of barley were 1 May, middle May and late May, respectively. In milk to yellow ripe stage, dry matter(DM) and total digestible nutrient(TDN) yield of barley were $7.30{\sim}9.58$ MT and $4.75{\sim}6.24$ MT per ha, and Albori was the most promising variety among the barley. The growth of wheat was a little late compared to that of barley. but it seemed to be crop having higher forage yield and nutritive value. In milk to yellow stage, DM and TDN yield of wheat were $8.17{\sim}10.82$ MT and $5.33{\sim}7.31$ MT per ha. Heading stage of rye was 27 to 29 April, and it seemed to be suitable for harvest at heading to flowering stage because of heavy lodging after flowering. In flowering stage, DM and TDN yield of rye were 10.18 and 6.03 MT per ha. Heading stage of early maturing IRG was 7 May and it seemed to be good for harvest at flowering stage of middle May in cropping system. In flowering stage, DM and TDN yield of early type IRG were 4.48 and 2.96 MT per ha. The results demonstrated that the promising forage crops for forage production in paddy. field were rye and barley(Albori) in Middle region, and rye harvesting at early May was recommended when considered middle May of rice transplanting period, and rye and barley considered late May of rice planting period. Also early maturing and high yielding IRG variety was strongly needed.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.24 no.3
• /
• pp.217-224
• /
• 2004

This study was carried out to select the promising forage crops for forage production in paddy field of HARI, RDA, Iksan from 1999 to 2001. The species of forage crop used in this experiment were barley(5 varieties), wheat(2), rye(3) and Italian ryegrass(IRG, 3). Stage of heading, milk and yellow ripe of barley were 26 $April{\sim}l$ May, middle May and late May, respectively. In milk to yellow ripe stage, dry matter(DM) yield of barley was $6.64{\sim}10.47$ MT per ha, and averaged ADF and NDF contents were $31.6\%\;and\;62.2\%$ respectively. Albori was the most promising variety among the barley). The growth of wheat was a little late compared to that of barley, but it seemed to be crop having higher forage yield md nutritive value. In milk to yellow stage, DM yield of wheat was $7.51{\sim}11.96$ MT per ha, and nutritive value of wheat was high(ADF $30.6\%$ and NDF $60.1\%$). Heading stage of rye was $25{\sim}27$ April, and it seemed to be suitable to harvest at heading to flowering stage because of heavy lodging after flowering. In flowering stage, DM yield of rye was 17.26 MT per ha, and nutritive value of rye was the lowest among 4 species(ADF $38.6\%$ and NDF $65.5\%$). Heading stage of early maturing IRG was 30 April and it seemed to be suitable for harvest at flowering stage of middle May in cropping system. In flowering stage, DM yield of early type IRG was 10.77 MT per ha, and averaged ADF and NDF contents were $31.6\%\;and\;59.2\%$, respectively. The results demonstrated that the promising forage crops for forage production in paddy field were rye, barley, early maturing IRG and wheat in Honam region. In Southern region, rye harvesting at early May was recommended when considered middle May of rice transplanting period, and barley(Albori) and early maturing IRG harvesting at middle May were recommended when considered late May of rice planting period. And also, wheat with high nutritive value and possibility for harvesting at late May were recommended in case of transplanting period at early June.

• Journal of Animal Environmental Science
• /
• v.18 no.sup
• /
• pp.81-90
• /
• 2012

This study was evaluated high end research grade Near infrared spectrophotometer (NIRS) to low end popular field grade multiple Near infrared spectrophotometer (NIRS) for rapid analysis at forage quality at sight with 241 samples of Italian ryegrass silage during 3 years collected whole country for evaluate accuracy and precision between instruments. Firstly collected and build database high end research grade NIRS using with Unity Scientific Model 2500X (650 nm~2,500 nm) then trim and fit to low end popular field grade NIRS with Unity Scientific Model 1400 (1,400 nm~2,400 nm) then build and create calibration, transfer calibration with special transfer algorithm. The result between instruments was 0.000%~0.343% differences, rapidly analysis for chemical constituents, NDF, ADF, and crude protein, crude ash and fermentation parameter such as moisture, pH and lactic acid, finally forage quality parameter, TDN, DMI, RFV within 5 minutes at sight and the result equivalent with laboratory data. Nevertheless during 3 years collected samples for build calibration was organic samples that make differentiate by local or yearly bases etc. This strongly suggest population evaluation technique needed and constantly update calibration and maintenance calibration to proper handling database accumulation and spread out by knowledgable control laboratory analysis and reflect calibration update such as powerful control center needed for long lasting usage of forage analysis with NIRS at sight. Especially the agriculture products such as forage will continuously changes that made easily find out the changes and update routinely, if not near future NIRS was worthless due to those changes. Many research related NIRS was shortly study not long term study that made not well using NIRS, so the system needed check simple and instantly using with local language supported signal methods Global Distance (GD) and Neighbour Distance (ND) algorithm. Finally the multiple popular field grades instruments should be the same results not only between research grade instruments but also between multiple popular field grade instruments that needed easily transfer calibration and maintenance between instruments via internet networking techniques.