• Applied Biological Chemistry
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• 제42권1호
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• pp.39-44
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• 1999

각각 다른 성장기의 한우 등심에서 추출한 단백질을 이차원 전기영동법으로 분리하여 젤 상의 단백질 전개 양상을 비교하였다. 성장 0, 6, 12, 24 개월령의 한우 등심 단백질들을 길이 16 cm 튜브젤에서 등전점에 따라 분리하고, 이차원적으로 $18{\times}20$ cm, 12% SDS-polyacrylamide gel 전기영동 하여 단백질을 분리하였다. 등전점 3.0에서 9.0 그리고 분자량 15,000에서 100,000 Da 사이의 단백질들이 분리되어 Silver 염색법으로 명확히 구분할 수 있었다. 흥미롭게, 성장과정에서 단백질 발현이 증가했거나 감소한 단백질들은 저분자 단백질들 이었다. 성장 과정 중 증가된 단백질들을 분리하기 위해 수용성 단백질들을 조직으로부터 1% Triton X-100 으로 추출하였다. 그리고 이를 30%와 50% 황산암모니아로 분획하였다. 이와 같이하여 각 단백질들의 분리조건을 결정하였다. 이들 조건을 이용하여 발현이 증가된 단백질들을 분리하고 PVDF membrane에 옮겨서 아미노산 서열을 결정하여 단백질을 규명하였다.

• 한국미생물·생명공학회지
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• 제48권2호
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• pp.167-178
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• 2020

Lipase-producing fungi have been isolated from environments containing lipids. The non-dairy creamer industrial waste has a high amount of lipids so it is a potential source for the isolation of lipase-producing fungi. However, the study of fungi that secrete lipase from this industrial waste has not been reported. The purpose of this study was to obtain lipase-producing filamentous fungi from non-dairy creamer industrial waste. Mineral salt and potato dextrose agar were used as media for the isolation process. The qualitative screening was conducted using phenol red agar medium and the quantitative screening using broth medium containing glucose and olive oil. Isolates producing the highest amounts of lipase were identified with molecular methods. We found that 5 out of 19 isolated filamentous fungi are lipase producers. Further analysis showed that isolate Ms.11 produced the highest amount of lipase compared to others. Based on ITS sequence Ms.11 was identified as Aspergillus aculeatus. The lipase activity in medium containing 1% glucose + 1% olive oil at pH 7.0 and 30℃ after 96 and 120 h of incubation was 5.13 ± 0.30 U/ml and 5.22 ± 0.59 U/ml, respectively. The optimum lipase activity was found at pH 7.0, 30℃ and using methanol or ethanol in the reaction tube. Lipase was more stable at 20-30℃ and maintained 85% of its activity. It was concluded that isolate Ms.11 is a potential source of lipase that catalyzes transesterification reactions. Further studies are required to optimize lipase production to make the strain suitable for industry purposes.

• Earthquakes and Structures
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• 제27권1호
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• pp.17-29
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• 2024

The traditional double story isolated structure is a derivative of the base isolated and inter-story isolated structures, while the new double story isolated structure represents a novel variation derived from the traditional double story isolated structure. In order to investigate the seismic response of the new double story isolated structure, a comprehensive structural model was developed. Concurrently, models for the basic fixed, base isolated, inter-story isolated, and traditional double story isolated structures were also established for comparative analysis. The nonlinear dynamic time-history response of the new double story isolated structure under rare earthquake excitations was analyzed. The findings of the study reveal that, in comparison to the basic fixed structure, the new double story isolated structure exhibits superior performance across all evaluated aspects. Furthermore, when compared to the base isolated and inter-story isolated structures, the new double story isolated structure demonstrates significant reductions in inter-story shear force, top acceleration, and inter-frame displacement. The horizontal displacement of the new double story isolated structure is primarily localized within the two isolation layers, effectively dissipating the majority of input seismic energy. In contrast to the traditional double story isolated structure, the new design minimizes displacements within the inter-isolation layer situated in the central part of the frame, as well as mitigates the overturning forces acting on the lower frame column. Consequently, this design ensures the structural integrity of the core tube, thereby preventing potential collapse and structural damage.

• 생약학회지
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• 제35권2호통권137호
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• pp.110-115
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• 2004

For the evaluation of anti-tumor activity of Korean Oldenlandia Herb (KOH) and Radix (KOR), our experiment was performed with methanol extracts of KOH and KOR. They did not shown any cytotoxicity against HT1080 cell lines. However, they effectively showed anti-metastatic activity through inhibition of the adhesion of HT1080 cells to gelatin, downregulated the expression of MMP2 and uPA and upregulated the expression of TIMP2. They also inhibited tube formation of HUVECs induced by bFGF. However, they did not affect DNA topoisomerase I activity. Simiarly, the T/Cs % in KOH and KOR treated mice were increased 134.9% and 171 %, respectively at 2500 mg/kg. These results suggest that KOH and KOR exert anti-tumor activity via anti-metastatic and anti-angiogenic activities. The further study for isolation of effective compounds and its exact mechanism and comparative study with Chinese Oldenlandia Herba will be required.

• Applied Biological Chemistry
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• 제28권2호
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• pp.98-105
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• 1985

소와 양의 Rumen에서 Hungate's roll tube 방법에 의해 통성 혐기성 섬유소 분해세균을 분리하였다. 가장 강한 섬유소 분해력을 갖는 균주를 선발하여 형태학적 배양학적 생리학적 특성 및 전자현미경사진을 검토하여 Cellulomonas fimi C-14으로 동정(同定)하였다. 분리된 C. fimi C-14의 균생육 및 효소생산은 $30^{\circ}C$, pH6.5에서 최대이었으며, 그의 조효소(組酵素)는 pH6.0, $40^{\circ}C$에서 최대 역가를 보였으며 그 $C_1,\;C_x,\;{\beta}-glucosidase$의 Activity 는 20.6, 227.6, 0.56 $(unit{\times}10^3/ml)$ 이었다. 곰팡이 Cellulase의 첨가로 Enzyme Activity가 증가하였으며, 현사시나무의 톱밥을 기질로 하여, 분리균과 S. cerevisiae DY2를 접종하여 Ethanol 발효생산시 ${\beta}-glucosidase$의 첨가로 상승효과를 보였으며, 이단계 발효법보다 동시 당화 발효법에 의한 Ethanol 생산이 더 좋았다.

• 대한수의학회지
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• 제45권2호
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• pp.279-285
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• 2005

One-lung ventilation (OLV) is the isolation and selective ventilation of one lung field. OLV allows the collapse of lung lobes on the side of the thoracic surgical approach to facilitate observation of intrathoracic structures and to achieve lung immobility. OLV be achieved by endotracheal intubation with double lumen tubes or bronchial blockers. In this study, cardiopulmonary consequences of two-lung ventilation (TLV), OLV and Re-TLV (TLV after OLV) were evaluated in 5 dogs. The dogs were anesthetized with mask induction and maintained with isoflurane in oxygen. Tidal volume and respiratory rates were set to maintain end-tidal $CO_2$ at $40{\pm}2mmHg$ during instrumentation. Following instrumentation, the dogs were placed in right lateral recumbency and induced spontaneously respiration state. Effect of TLV on hemodynamic and pulmonary variables were recorded. Then, the left bronchus was obstructed by endotracheal intubation with double lumen endotracheal tube to achieve OLV state and recording was continued. After OLV, double lumen endotracheal tube was extubated, and standard endotracheal tubes was intubated again. In this study, spontaneous OLV caused significant decrease in $PaO_2$, arterial oxygen saturation, mixed-venous oxygen saturation, and increase in $PaCO_2$. Especially, a significant elevation in $PaCO_2$ and respiratory acidosis were remarkable findings. So spontaneous ventilation in OLV affected gas exchange and hemodynamic function.

• 분석과학
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• 제22권5호
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• pp.395-406
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• 2009

사용 후 핵연료시료 중의 요오드를 정량하고 용해과정 중 요오드의 휘발거동을 조사하기 위하여 중성자 방사화 분석(NAA) 및 전자미세탐침분석(EPMA)을 이용하였다. 모의 사용 후 핵연료시료(SIMFUELs)를 준비하여 $HNO_3$(1+1) 용액으로 $90^{\circ}C$에서 8시간 용해하고 용해 후 용해용액 중에 잔류된 요오드, 용해장치에 응축된 요오드 및 휘발하여 흡착체에 포집된 요오드 각각을 정량하였다. 응축된 요오드는 장치내 용해용액을 옮긴 후 $HNO_3$(1+1) 용액으로 재증류하여 회수하였다. 용해 및 재증류 용액중의 요오드는 용매추출과 이온교환 및 침전법으로 분리한 후 방사화학적 중성자 방사화 분석(RNAA)으로 정량하였다. 요오드 분리에 사용한 이온교환분리관 및 여과키트는 폴리에틸렌 관으로 제작하여 중성자 조사를 위한 이송관 내부의 삽입체(Insert)로 이용하였다. 핵연료용해 중 휘발된 요오드는 제조한 흡착체(Ag-Silica gel)를 담은 흡착관에 포집하였다. 흡착체를 구간별로 나누어 균질시료로 만든 다음 비파괴 중성자 방사화 분석(INAA)으로 정량하였다. 흡착된 요오드의 분포를 EPMA 분석으로 조사하였다. 모의 사용 후 핵연료 및 원자력발전소로부터의 실제 사용 후 핵연료 시료로부터 휘발된 요오드의 흡착특성을 비교하였다.

• 한국환경과학회지
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• 제12권12호
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• pp.1293-1301
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• 2003

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the 47 point at west and south coast of Korea in September 2001. Separated 13 samples of changes with red color under 28-32$^{\circ}C$, 3000 lux, anaerobe conditions for 7 days cultivated in basal medium. For pure isolation from 13 samples, we used agar-shake tube method (0.4 ％ agar) and separated 5 strains through 13-repetition test. EGH-24 and EGH-30 was identified as the same strain through the RAPD(Random Amplified Polymorphic DNA)-PCR of strain EGH-9, EGH-13, EGH-23, EGH-24, EGH-30. Four isolates cultivated in synthesis wastewater for wastewater biodegradation test. EGH-24 was selected with efficient wastwater treating strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 16S-rDNA phylogenetic analysis, EGH-24 strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.

• 대한미생물학회지
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• 제11권1호
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• pp.79-85
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• 1976

There are many of anaerobic culture methods and equipments for isolation and cultivation of anaerabic bacteria, but most of these methods are used without pre-reduced media. Gas-jet method is a recommend. able method for the culture of anaerobes, resently developed. Bacteriological studies were experimented of liver abscess of cattle by the use of gas. jet method. The results were summarised as follows; 1. Gas-jet method for anaerobic culture are expedient for the making of pre-reduced media, maintaining of oxygen free condition in the culture tube, picking of bacteria from colony and colony counting etc. 2. A 121 strains of facultative anaerobic and anaerobic bacteria were isolated from liver abscess of 27 head of cattle, and the isolated anaerobic bacteria were as follows. Peptostreptococcus spp. 7 strains Acid aminococcus fermentans 1 Veillonella spp. 1 Bacterioides spp. 6 Bifidobacterium spp. 4 Arachinia propionica 2 Lactobacillus spp. 4 Propionibacterium acnes 1 3. Liver abscess were infected with many of bacteria, about $10^3-10^9$ numbers per gram of abcessed tissue. Almost of abscess were mixed infection of various bacterial species rather than simple species.

• The Plant Pathology Journal
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• 제25권3호
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• pp.291-293
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• 2009

The low virus titer in woody plant tissues and the presence of inhibitor compounds such as polyphenols, tannins and polysaccharides are common difficulties that compromise purification of plant viroids from their woody hosts. A simple, reliable method of RNA isolation using CF11 cellulose column on a microcentrifuge tube scale for detecting Apple scar skin viroid (ASSVd) in apple was developed. Total RNA extracted from leaf, woody bark and the fruit skin was used for reverse transcription. RT-PCR products could be detected from RNA prepared from dormant woody bark, fruit skin and fresh leaves with both the CF11 cellulose column method and NucliSens extractor in February, August and November. Meanwhile, with the RNeasy kit RT-PCR, products were detected only in leaves and not from bark or fruit skin. The PCR product, about 330 base pairs, was analyzed by agarose gel electrophoresis. The CF11 cellulose column method was effective for detecting ASSVd. The method enabled the processing of a large numbers of samples of dormant woody bark, leaf and fruit skin of apple.