• Proceedings of the Korean Geotechical Society Conference
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• 1998.05a
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• pp.83-114
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• 1998

The most widely accepted method for understanding the deformation mechanism of rock is from the use of computer simulation. However, if the changes in rock properties after excavation are significant this will prevent the computer simulation kent predicting the deformation with acceptable accuracy. If the deformations are, however, carefully measured in situ, the resulting data can be more useful far predicting the deformational behavior of underground openings, since the effect of the parameters which influence the deformational behavior are included in the measurement. In this study, extensive data analyses were carried out using the deformation measurements from the Waste Isolation Pilot Plant (WIPP), which is a permanent nuclear waste repository The results from computer simulations were compared with field measurements to evaluate the assumptions used in the computer simulations, For better description of the deformational behavior around underground excavations, several techniques were developed, namely: (a) the calculation of the zero strain boundary; (b) the evaluation of the influence of adjacent excavations on the deformational behavior of pre-excavated openings; (c) the description of the deformational behavior using in situ measurements; (d) the calculation of the shear stress distribution; and (e) the application of a Neural Network for the prediction of opening deformation.

• Bulletin of the Korean Chemical Society
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• v.28 no.8
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• pp.1289-1293
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• 2007

Persicaside has been isolated as a new alkaloid natural compound from a methanol (EtOA)-soluble extract of Prunus persica seed. It was purified by a combination of chromatographic techniques and recrystallization. The structure of Persicaside was determined by extensive NMR experiments and mass ppectroscopic data. It inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) production via suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2 expression in rat osteoblast sarcoma cells (ROS 17/2.8) in concentration-dependent manner whereas it spares the COX-1 enzyme activity.

• Mycobiology
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• v.42 no.4
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• pp.311-316
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• 2014

Lichen studies, including biodiversity, phylogenetic relationships, and conservation concerns require definitive species identification, however many lichens can be challenging to identify at the species level. Molecular techniques have shown efficacy in discriminating among lichen taxa, however, obtaining genomic DNA from herbarium and fresh lichen thalli by conventional methods has been difficult, because lichens contain high proteins, polysaccharides, and other complex compounds in their cell walls. Here we report a rapid, easy, and inexpensive protocol for extracting PCR-quality DNA from various lichen species. This method involves the following two steps: first, cell breakage using a beadbeater; and second, extraction, isolation, and precipitation of genomic DNA. The procedure requires approximately 10 mg of lichen thalli and can be completed within 20 min. The obtained DNAs were of sufficient quality and quantity to amplify the internal transcribed spacer region from the fungal and algal lichen components, as well as to sequence the amplified products. In addition, 26 different lichen taxa were tested, resulting in successful PCR products. The results of this study validated the experimental protocols, and clearly demonstrated the efficacy and value of our KCl extraction method applied in the fungal and algal samples.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2008.11a
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• pp.638-641
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• 2008

Design is an iterative, generative, and multidisciplinary process by its nature. Iteration is frequent in most of the engineering design and development projects including construction. Design iterations cause rework, and extra efforts are required to get the optimal sequence and to manage the projects. Contrary to simple design, isolation of the generative iterations in complex design systems is very difficult, but reduction in overall iterations is possible. Design depends upon the information flow within domain and also among various design disciplines and organizations. Therefore, it is suggested that managers should be aware about the crucial iterations causing rework and optimal sequence as well. In this way, managers can handle design parameters related to such iterations proactively. Numbers of techniques are available to reduce iterations for various kinds of engineering designs. In this paper, parameter based Design Structure Matrix (DSM) is chosen. To create this DSM, a survey was performed and then partitioned using a model. This paper provides an easy approach to those companies involved in or intend to be involved in "design and build projects."

• Child Health Nursing Research
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• v.12 no.3
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• pp.368-376
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• 2006

Purpose: The purpose of this research was to examine the degree of care stress and the quality of life of grandmothers caring for their grandchildren while mother was employed. Method: A convenience sample of 107 grandmothers were measured for grandmother's care stress using a revised for of the PSI/SF and the quality of life tool by Andrew and Weinert. Descriptive statistics, t-test, and Pearson correlation coeffcients were used to analyze the data using SPSS/PC 10.0 Results: The results found that grandmothers' care stress was high according to their age and religion. Their quality of life was low according to their age, their present health status, income and sleep pattern. Their subjective care stress was a sense of social isolation, burden of caring for grandchildren, health problems, and conflict with adult children. Care stress and quality of life were found to be negatively correlated. This result was statistically significant. Conclusion: In conclusion, it was found that the care stress was high and affected quality of life. From this study it is proposed that there is a need to develop programs which consider grandmother' techniques in child care and health care to improve their quality of life. Ultimately, a good care environment for children will be good for the children's growth and development.

• Applied Biological Chemistry
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• v.47 no.2
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• pp.270-273
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• 2004

Two prenylated flavonoids were isolated from a chloroform extract of the root bark of Cudrania tricuspidata. Both compounds (1, 2) showed antibacterial activity against Gram positive bacteria, Staphylococcus aureus, Bacillus subtilis and Bacillus cereus. Their structures were determined as euchrestaflavanone B (1) and euchrestaflavanone C (2) on the basis of $^1H\;NMR,\;^{13}C\;NMR$ and long-range coupling NMR techniques.

• Molecular & Cellular Toxicology
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• v.2 no.1
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• pp.11-14
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• 2006

In this study we attempted to develop a novel genomic method to selectively isolate target functional microbial genomes from environmental samples. For this purpose, stable isotope probing (SIP) was applied in selectively isolating organic pollutant-assimilating populations. When soil microbes were fed with $^{13}C-labeled $ biphenyl, biphenyl-utilizing cells were incorporated with the heavy carbon isotope. The heavy DNA portion was successfully separated by CsCl equilibrium density gradient. And the diversity in the heavy DNA was sufficiently reduced, being suitable for the current DNA microarray techniques to detect biphenyl-utilizing populations in the soil. In addition, we proposed a new way to get more genetic information by combining this SIP method with selective metagenomic approach. The increased selective power of these new DNA isolation methods will be expected to provide a good quality of new genetic information, which, in turn, will result in development of a variety of biomarkers that may be used in assessing ecotoxicology issues including the impacts of organic hazards, and antibiotic-resistant pathogens on human and ecological systems.

• ETRI Journal
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• v.43 no.3
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• pp.414-426
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• 2021

In the task of data routing in Internet of Things enabled volatile underwater environments, providing better transmission and maximizing network communication performance are always challenging. Many network issues such as void holes and network isolation occur because of long routing distances between nodes. Void holes usually occur around the sink because nodes die early due to the high energy consumed to forward packets sent and received from other nodes. These void holes are a major challenge for I-UWSANs and cause high end-to-end delay, data packet loss, and energy consumption. They also affect the data delivery ratio. Hence, this paper presents an energy efficient watchman based flooding algorithm to address void holes. First, the proposed technique is formally verified by the Z-Eves toolbox to ensure its validity and correctness. Second, simulation is used to evaluate the energy consumption, packet loss, packet delivery ratio, and throughput of the network. The results are compared with well-known algorithms like energy-aware scalable reliable and void-hole mitigation routing and angle based flooding. The extensive results show that the proposed algorithm performs better than the benchmark techniques.

• Human Ecology Research
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• v.57 no.1
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• pp.73-89
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• 2019

This study explores the social phenomenon of the universalization of one-person households through a literature analysis and text mining in order to explore a future direction for Home Economics Education(HEE) development in the one-person household era. From 2010 to 2018, texts from newspaper articles and book content of one-person households were analyzed through R program. The results of the study are as follows. In order to develop students' competency to live a happy life in the one-person household era, it is necessary to: (1) expand the preemptive and collaborative research of HEE, (2) develop and operate a curriculum to raise the living competency to live alone, (3) expand opportunities for secondary school students as well as off-campus youth, middle-aged, and elderly students, and (4) develop various HEE's elective curriculum focusing on the ability to live as one-person household. Also, (5) in order to overcome the psychological and social poverty and isolation of one-person households, HEE should strengthen the learner's ability to form relationships through self-esteem, care of others, community life, communication and conflict resolution education. In conclusion, HEE's independent living competency, relationship formation competency, and practical problem solving competency are all necessary competencies to live in one-person households. In this study, it is meaningful to suggest a future direction for HEE and to use new research methods such as word cloud techniques in the absence of HEE's previous research in relation to the increase of one-person households.

• BMB Reports
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• v.52 no.9
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• pp.540-547
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• 2019

Immune repertoire is a collection of enormously diverse adaptive immune cells within an individual. As the repertoire shapes and represents immunological conditions, identification of clones and characterization of diversity are critical for understanding how to protect ourselves against various illness such as infectious diseases and cancers. Over the past several years, fast growing technologies for high throughput sequencing have facilitated rapid advancement of repertoire research, enabling us to observe the diversity of repertoire at an unprecedented level. Here, we focus on B cell receptor (BCR) repertoire and review approaches to B cell isolation and sequencing library construction. These experiments should be carefully designed according to BCR regions to be interrogated, such as heavy chain full length, complementarity determining regions, and isotypes. We also highlight preprocessing steps to remove sequencing and PCR errors with unique molecular index and bioinformatics techniques. Due to the nature of massive sequence variation in BCR, caution is warranted when interpreting repertoire diversity from error-prone sequencing data. Furthermore, we provide a summary of statistical frameworks and bioinformatics tools for clonal evolution and diversity. Finally, we discuss limitations of current BCR-seq technologies and future perspectives on advances in repertoire sequencing.