• Title/Summary/Keyword: isolation systems

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Isolation and Characterization of an Immunopotentiating Factor from Lactobacillus plantarum in Kimchi: Assessment of Immunostimulatory Activities

  • Lee, Jong-Hwa;Kweon, Dae-Hyuk;Lee, Seung-Cheol
    • Food Science and Biotechnology
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    • v.15 no.6
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    • pp.877-883
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    • 2006
  • The immunostimulatory activities of Lactobacillus plantarum, the major microorganism in kimchi fermentations were investigated. Five strains of L. plantarum exhibited weak immunopotentiating activity, but L. plantarum PS-21 showed as strong a mitogenic activity as Bifidobacterium adolescentis M101-4, a known positive strain. It is of interest that, L. plantarum PS-21 stimulated proliferation of Peyer's patch cells, one of the most important tissues in the gut-associated lymphoreticular system. Cell' wall fractions from L. plantarum PS-21 also showed strong mitogenic activity compared with the soluble cytoplasmic fraction. A peptidoglycan fraction (PG) extracted from the cell wall of L. plantarum PS-21 was identified as an active mitogenic component when used in murine lymph node and spleen cell test systems. PG showed dose-dependent mitogenic activity and significantly enhanced antibody production in lymph node cells when studied in vitro. The lysosomal enzyme activity of murine peritoneal macrophages was increased when analyzed following injection of PG to the host animal. Furthermore, PG enhanced the production of cytokines such ($TNF-{\alpha}$ and IL-6) in the in vitro culture of RAW 264.7 macrophage cells.

Seismic behavior of structures isolated with a hybrid system of rubber bearings

  • Chen, Bo-Jen;Tsai, C.S.;Chung, L.L.;Chiang, Tsu-Cheng
    • Structural Engineering and Mechanics
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    • v.22 no.6
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    • pp.761-783
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    • 2006
  • The enlargement of interest in base isolators as an earthquake-proof design strategy has dramatically accelerated experimental studies of elastomeric bearings worldwide. In this paper, a new base isolator concept that is a hybrid system of rubber bearings is proposed. Uniaxial, biaxial, and triaxial shaking table tests are also performed to study the seismic behavior of a 0.4-scale three-story isolated steel structure in the National Center for Research on Earthquake Engineering in Taiwan. Experimental results demonstrate that structures with a hybrid system of rubber bearings composed of stirruped rubber bearings and laminated rubber bearings can actually decrease the seismic responses of the superstructure. It has been proved through the shaking table tests that the proposed hybrid system of rubber bearings is a very promising tool to enhance the seismic resistance of structures. Moreover, it is demonstrated that the proposed analytical model in this paper can predict the mechanical behavior of the hybrid system of rubber bearings and seismic responses of the base-isolated structures.

Solidification Analysis Characteristics of Back Flow Prevention Check Valve (역류방지 체크밸브의 응고해석 특성)

  • Yoon, Jung-In;Moon, Jung-Hyun;Son, Chang-Hyo;Lee, Jung-Jin
    • Journal of Power System Engineering
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    • v.19 no.3
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    • pp.69-74
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    • 2015
  • Check valves used in vessels include shock-release function on piping system, aside from basic back flow prevention. However, proper and enough protection of system is not obtainable due to use of high-pressure and bulk fluids, resulting from enlargement of vessels. In this study, casting analysis of check valves protecting systems in flow path from water hammering or back flow is conducted, using Z-CAST program. Also, molten metal filling, flow analysis, solidification analysis and shrinkage cavity analysis are conducted. The main results are as following. Regarding filling of each risering, molten metal showed stable supply condition without being isolated. It was identified that the final solidification exists on risering, but shrinkage cavity possibly might happens at the point of isolation solidification.

Design of Elastomeric Bearing System and Analysis of it Mechanical Properties

  • Moon, Byung-Young;Kang, Gyung-Ju;Kang, Beom-Soo;Cho, Dae-Seung
    • Journal of Mechanical Science and Technology
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    • v.18 no.1
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    • pp.20-29
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    • 2004
  • This paper proposes a new type of bearing system. In this study, a method for design of on elastomeric bearing system and its mechanical property analysis are carried. Experimental and theoretical studies of the elastomeric bearings with fiber reinforcement were proved effective new lightweight bearing system. The fibers in the bearings for isolation are assumed to be flexible in extension, in contrast to the steel plates in the conventional bearing system. Several kinds of bearing systems in the form of long strips are designed, fabricated and tested. The results suggest that it is possible to produce the economical and effective fiber-reinforced elastomeric bearing that matches the behavior of a steel-reinforced bearing. Feasibility and advantages of the proposed bearings are illustrated by the application of the analytic procedure to the structure system. Results obtained here are reported to be an efficient approach with respect to bearing system and design of bearing against shock absorbing system when compared with other conventional one.

Preparedness of Siddha system of medicine in practitioner perspective during a pandemic outbreak with special reference to COVID-19

  • Rajalakshmi, S.;Samraj, K.;Sathiyarajeswaran, P.;Kanagavalli, K.
    • CELLMED
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    • v.10 no.4
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    • pp.29.1-29.6
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    • 2020
  • COVID-19 (Corona Virus Disease-2019) is an infectious respiratory disease caused by the most recently discovered coronavirus, SARS-CoV-2 (Severe Acute Respiratory Syndrome Corona virus-2). This new viral disease was unknown before the outbreak began in Wuhan, China, in December 2019. As of November 16th 2020, it affects about 54.3 million populations, death troll increased to 1.32 million cases in worldwide. Whereas in India 8.85 cases are infected with COVID-19, of which 1, 30, 112 cases were died. Till now there has been no specific anti-virus drug or vaccines are available for the treatment of this disease, the supportive care and non-specific treatment to the symptoms of the patient are the only options in Biomedicine, the entire world turns its attention towards alternative medicine or Traditional medicine. Siddha medicine is one of the primordial systems of medicine practiced in the southern part of India, it dealt a lot about pandemic, and its management. This review provides an insight into Pandemic in Siddha system and its management in both ancient history and modern history, National and state level Government policies related to current pandemic, World Health Organization (WHO) guidelines on usage of unproven drug during infectious disease outbreak, Preparedness of Siddha system during a pandemic outbreak Challenges and Recommendations.

Etching of Silicon Wafer Using Focused Argon lon Laser Beam (집속 아르곤 이온 레이저 빔을 이용한 실리콘 기판의 식각)

  • Cheong, Jae-Hoon;Lee, Cheon;Park, Jung-Ho
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.48 no.4
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    • pp.261-268
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    • 1999
  • Laser-induced thermochemical etching has been recognized as a new powerful method for processing a variety of materials, including metals, semiconductors, ceramics, insulators and polymers. This study presents characteristics of direct etching for Si substrate using focused argon ion laser beam in aqueous KOH and $CCl_2F_2$ gas. In order to determine process conditions, we first theoretically investigated the temperature characteristics induced by a CW laser beam with a gaussian intensity distribution on a silicon surface. Major process parameters are laser beam power, beam scan speed and reaction material. We have achieved a very high etch rate up to $434.7\mum/sec$ and a high aspect ratio of about 6. Potential applications of this laser beam etching include prototyping of micro-structures of MEMS(micro electro mechanical systems), repair of devices, and isolation of opto-electric devices.

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Study on Satellite Vibration Control using Adaptive Control Scheme

  • Oh, Se-Boung;Oh, Choong-Seok;Bang, Hyo-Choong
    • International Journal of Aeronautical and Space Sciences
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    • v.6 no.2
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    • pp.1-16
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    • 2005
  • Adaptive control methods are studied for the Satellite to isolate vibration in spite of the nonlinear system dynamics and parameter uncertainties of disturbance. First, a centralized control scheme is developed based on the particle swarm optimization(PSO) algorithm and feedback theory to automatically tune controller gains. A simulation study of a 3 degree-of-freedom device was conducted to evaluate the performance of the proposed control scheme. Next, since a centralized control scheme is hard to construct model dynamics and not goad at performance when controller and systems environment are easily changed, a decentralized control scheme is presented to avoid these defects of the centralized control scheme from the point of view of production and maintenance. It is based on the adaptive control methodologies to find PID controller parameters. Experiment studies were conducted to apply the adaptive control scheme and evaluate the performance of the proposed control scheme with those of the conventional control schemes.

Wafer-Level Package of RF MEMS Switch using Au/Sn Eutectic Bonding and Glass Dry Etch (금/주석 공융점 접합과 유리 기판의 건식 식각을 이용한 고주파 MEMS 스위치의 기판 단위 실장)

  • Kang, Sung-Chan;Jang, Yeon-Su;Kim, Hyeon-Cheol;Chun, Kuk-Jin
    • Journal of Sensor Science and Technology
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    • v.20 no.1
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    • pp.58-63
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    • 2011
  • A low loss radio frequency(RF) micro electro mechanical systems(MEMS) switch driven by a low actuation voltage was designed for the development of a new RF MEMS switch. The RF MEMS switch should be encapsulated. The glass cap and fabricated RF MEMS switch were assembled by the Au/Sn eutectic bonding principle for wafer-level packaging. The through-vias on the glass substrate was made by the glass dry etching and Au electroplating process. The packaged RF MEMS switch had an actuation voltage of 12.5 V, an insertion loss below 0.25 dB, a return loss above 16.6 dB, and an isolation value above 41.4 dB at 6 GHz.

A Web Server Load Balancing Mechanism for Supporting Service Level Agreement (SLA를 지원하는 웹 서버 부하 분산 기법)

  • Go Hyeon-Joo;Park Kie-Jin;Park Mi-Sun
    • Journal of KIISE:Computer Systems and Theory
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    • v.33 no.8
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    • pp.505-513
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    • 2006
  • To satisfy SLA(Service Level Agreement) contract between a client and a service provider, the client requests are classified and processed by priorities. In providing differentiated service, a request from a client who has low priority can be dealt with less important. In this paper, we study static and dynamic partitioning mechanism of web servers and also admission control policy for multiclass request distribution. Through simulation experiments, we analyze web server throughput and response time considering SLA.

Molecular Characterization of a Protein Kinase Gene in Chiness Cabbage(Brassica campestrics subsp. napus var. pekinensis)

  • Jeong, Sang-Ho;Ahn, Ji-Hoon;Lee, June-Seung;Lee, Jong-Seob
    • Animal cells and systems
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    • v.1 no.1
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    • pp.135-142
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    • 1997
  • Random sequencing of expressed sequence tags in roots of Chinese cabbage led to isolation of a partial cDNA clone, BR77, which encoded a putative protein kinase. Using the BR77 cDNA as a probe, we isolated a full-length cDNA encoding the Brassica campestris protein kinase 1 (Bcpk1). The Bcpt1 cDNA contained one open reading frame encoding a polypeptide of 439 amino acids. The putative polypeptide consisted of a short N-terminal region and a protein kinase catalytic domain. The catalytic domain of Bcpkl showed a high homology to cAMP- and calcium- phospholipid-dependent subfamilies of serine/threonine protein kineses. Eleven major catalytic domains in protein kineses were well conserved in Bcpk1. However, Bcpk1 contained a unique nonhomologous intervening sequence between subdomains VII and VIII, which was not found in protein kineses of animals and lower eukaryotes. Genomic DNA gel blot analysis showed that Bcpt1 genes might be present as three copies in the Chinese cabbage genome. These imply that Bcpk1 belongs to a plant-specific serine/threonine protein kinase subfamily.

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