• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.29-29
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• 2015

Clubroot disease is caused by the soil-born obligate plant pathogen Plasmodiophora brassicae. This pathogen can infect all cruciferous vegetables and oil crops, including Brassica rapa, B. oleracea, B. napus, and other Brassica species. Clubroot disease is now considered to be a major problem in Chinese cabbage production in China, Korea, and Japan. We collected several hundreds of P. brassicae infected galls from Korea, and isolated the single spore from the collection. For establishment of novel isolation, and mass-propagation methods for singe spore isolates of P. brassicae pathogen, we developed new filtration method using both cellulose nitrate filter and syringe filter. Accurate detection of P. brassicae pathogen in the field was done by using real-time PCR in the potential infested soil. When we tested the different pathogenicity on commercial Chinese cabbage varieties, P. brassicae from collected galls showed various morphological patterns about clubroot symptom on roots. To date, 8 CR loci have been identified in the B. rapa genome using the quantitative trait loci (QTL) mapping approach, with different resistant sources and isolates. We are trying to develop the molecular marker systems for detect all 8 CR resistant genes. Especially for the study on the interaction between pathogens and CR loci which are not well understood until now, genome wide association studies are doing using the sequenced inbred lines of Chinese cabbage to detect the novel CR genes.

• Korean Journal of Computational Design and Engineering
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• v.17 no.2
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• pp.97-110
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• 2012

High performance sensors and modern data logging technology with real-time telemetry facilitate system fault diagnosis in a very precise manner. Fault detection, isolation and identification in fault diagnosis systems are typical steps to analyze the root cause of failures. This systematic failure analysis provides not only useful clues to rectify the abnormal behaviors of a system, but also key information to redesign the current system for retrofit. The main barriers to effective failure analysis are: (i) the gathered data (event) logs are too large in general, and further (ii) they usually contain noise and redundant data that make precise analysis difficult. This paper therefore applies suitable pre-processing techniques to data reduction and feature extraction, and then converts the reduced data log into a new format of event sequence information. Finally the event sequence information is decoded to investigate the correlation between specific event patterns and various system faults. The efficiency of the developed pre-filtering procedure is examined with a terminal box data log of a marine diesel engine.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.3
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• pp.411-418
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• 1992

Recently, onset of bacterial disease, especially infiltration of chronic respiratory diseases have been increasing at high rate. The main cause of these diseases are originated from inappropriate sanitary management and slow progress in introducing system and herd free system, which are the base of productivity improvement in bigger pig management, Methods for the prevention and removal of these diseases, are divided into four categories. The first category includes prevention and removal of infectious diseases by organizing strict animal quarantine, enforcing vaccination, and legal regulation and disposal at the time of outbreak. The second category includes improvement of production systems. This purpose can be achieved by discontinuing the open herd system, which brings on the invasion of foreign pathogens and replacing the system by the closed type of farm. To Continue eradication process step-wisely by performing the all-in all-out system at every pig pen or herd level is also effective for this purpose. The third category includes introduction of herd free system can be achieved by repopulating conventional pigs with SPF pigs. If these means are unrealizable from economic or technical viewpoint, medicated early weaning system is perfomed. This system consists of disinfection of pigsty at pre-postpartum stage, application of drugs to pregnant sows and newborn piglets in accordance with the medication program, and early weaning and rearing of newborn piglets in isolation. The fourth category is sanital leading by veterinarians, aimed to eliminating incentives for diseases through diagnoses. Their main activities include periodical monitoring and improvement leading on each responsible subject.

• Smart Structures and Systems
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• v.20 no.1
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• pp.23-33
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• 2017

Variable Curvature Friction Pendulum (VCFP) bearing is one of the alternatives to control excessive induced responses of isolated structures subjected to near-fault ground motions. The curvature of sliding surface in this isolator is varying with displacement and its function is non-spherical. Selecting the most appropriate function for the sliding surface depends on the design objectives and ground motion characteristics. To date, few polynomial functions have been experimentally tested for VCFP however it needs comprehensive parametric study to find out which one provides the most effective behavior. Herein, seismic performance of the isolated structure mounted on VCFP is investigated with two different polynomial functions of the sliding surface (Order 4 and 6). By variation of the constants in these functions through changing design parameters, 120 cases of isolators are evaluated and the most proper function is explored to minimize floor acceleration and/or isolator displacement under different hazard levels. Beside representing the desire sliding surface with adaptive behavior, it was shown that the polynomial function with order 6 has least possible floor acceleration under seven near-field ground motions in different levels.

• Smart Structures and Systems
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• v.16 no.4
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• pp.725-742
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• 2015

Tuned mass dampers (TMD) are devices employed in vibration control since the beginning of the twentieth century. However, their implementation for controlling the seismic response in civil structures is more recent. While the efficiency of TMD on structures under far-field earthquakes has been demonstrated, the convenience of its employment against near-fault earthquakes is still under discussion. In this context, the study of this type of device is raised, not as an alternative to the seismic isolation, which is clearly a better choice for new buildings, but rather as an improvement in the structural safety of existing buildings. Seismic records with an impulsive character have been registered in the vicinity of faults that cause seismic events. In this paper, the ability of TMD to control the response of structures that experience inelastic deformations and eventually reach collapse subject to the action of such earthquakes is studied. The results of a series of nonlinear dynamic analyses are presented. These analyses are performed on a numerical model of a structure under the action of near-fault earthquakes. The structure analyzed in this study is a steel frame which behaves as a single degree of freedom (SDOF) system. TMD with different mass values are added on the numerical model of the structure, and the TMD performance is evaluated by comparing the response of the structure with and without the control device.

• Smart Structures and Systems
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• v.16 no.5
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• pp.961-980
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• 2015

Magnetorheological (MR) fluids are capable of changing their rheological properties under the application of external fields. When MR fluids operate in the so-called squeeze mode, in which displacement levels are limited to a few millimetres but there are large forces, they have many potential applications in vibration isolation. This paper presents an experimental and a numerical investigation of the performance of an MR fluid under tensile and compressive loads and oscillatory squeeze-flow. The performance of the fluid was found to depend dramatically on the strain direction. The shape of the stress-strain hysteresis loops was affected by the strength of the applied field, particularly when the fluid was under tensile loading. In addition, the yield force of the fluid under the oscillatory squeeze-flow mode changed almost linearly with the applied electric or magnetic field. Finally, in order to shed further light on the mechanism of the MR fluid under squeeze operation, computational fluid dynamics analyses of non-Newtonian fluid behaviour using the Bingham-plastic model were carried out. The results confirmed superior fluid performance under compressive inputs.

• Journal of Magnetics
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• v.21 no.3
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• pp.348-355
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• 2016

Precision motion stages used in the manufacturing process of flat-panel displays have inevitably low settling performance due to their huge mass and bulky structures. In order to improve the settling performance, several methods of frame vibration compensation have been developed so far. These methods are used to cancel the vibration by imposing a counter force or modifying the resonance mode of the frame of the stage. To compensate the frame vibration, high force actuators are required. In this paper, a mighty voice coil actuator is proposed to generate the counter force against the frame vibration. The proposed voice coil actuator has an axis-symmetric rectangular structure to achieve a large force with simple and low cost fabrication. Also, the voice coil actuator allows radial clearance up to ${\pm}4mm$. Using an optimized design process and a magnetic circuit model, the power consumption is minimized while the required force is obtained. With a power of 322 W, the VCA has been designed to have a maximum force of 574 N with a force constant of 164 N/A. Experimental results verified the force constant of the fabricated voice coil actuator is well matched with the designed value.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.89-92
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• 2001

Aspergillus niger has been used as a host system to express many heterologous proteins. It has various advantages over other expression systems in that it is a small eukaryotic GRAS (Generally Recognized aS Safe) organism with a capacity of secreting large amount of foreign proteins. However, it has been known that the presence of an abundant protease is a limiting factor to express a heterologous protein. The proteases deficient mutants of A. niger were obtained using UV -mutagenesis. A total of 1 ${\times}$ $10^5$ spores were irradiated with 10-20% survival dose of UV, 600J/M2 at 280nm, and the resulting spores were screened on the casein -gelatin plates. Ten putative protease deficient mutants were further analyzed on the starch plates to differentiate the pro from the secretory mutant. An endogenous extracellular enzyme, glucose oxidase, was also examined to confirm that the mutant phenotype was due to the proteases deficiency rather than the mutation in the secretory pathway. The reduced proteolytic activity was measured using SDS-fibrin zymography gel, casein degradation assay, and bio-activity of a supplemented hGM -CSF (human Granulocyte-Macrophage Colony Stimulating Factor). Comparing with the wild type strain, less than 30 % of proteolytic activity was observed in the culture filtrate of the protease deficient mutant (pro -20) without any notable changes in cell growth and secretion.

• Proceedings of the KIPE Conference
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• 2001.10a
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• pp.683-686
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• 2001

This paper presents a novel prototype of the utility­interactive voltage source type sinewave pulse modulated power inverter using a high-frequency flyback transformer link. The proposed power conditioner circuit for the solar photovoltaic generation and small scale fuel cell has an isolation function due to the safety of the power processing system, which is more cost effective and acceptable for the small-scale distributed renewal energy conditioning and processing systems. The discontinuous current mode(DCM) of this power processing conversion circuit is applied to implement a simple circuit topology and pulse modulated control scheme. Its operation principle is described on the basis of simulation evaluations and theoretical considerations. The simulation results obtained herein prove that the proposed inverter outputs with sinusoidal waveforms and unity power factor currents are synchronized to the main voltage in utility power source grid. In this paper, the soft switching topology of high­frequency linked sinewave pulse modulation inverter is proposed and discussed.

• KSBB Journal
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• v.30 no.6
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• pp.275-282
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• 2015

Dried samples of Ligustrum japonicum were extracted twice: with methylene chloride and with methanol (MeOH), respectively. The combined crude extracts were successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by liquid-liquid partition. Antioxidant activities of crude extract and its solvent fractions were evaluated by measuring authentic $ONOO^-$, and $ONOO^-$ generated from 3-morpholinsydnonimine (SIN-1) as well as degree of occurrence of intracellular ROS in HT 1080 cells, and genomic DNA oxidation. The 85% aq.MeOH and n-BuOH fractions exhibited the good antioxidant activity. Further purification of the 85% aq.MeOH fracition led to the isolation of Oleanolic acid (1), Maslinic acid (2), and Ursolic acid (3). All compounds showed the significant antioxidant effects in all assay systems.