• Animal cells and systems
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• v.2 no.2
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• pp.239-242
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• 1998

Arabidopsis trp1 mutant plants, deficient in phosphoribosyI anthranilate transferase (PAT) activity, accumulate anthranilate compounds, which render them blue fluorescence. The visible phenotype of trp1 makes the PAT gene an excellent reporter gene in the mutant. In order to develop a system for the homologous recombination using the phenotypic characteristic of trp1-100, we established optimum conditions for the isolation and regenera tion of protoplast from auxin-conditioned, trp1-100 root cultures. Trvptophan had to be supplemented in the germination medium for the efficient cell division and subsequent plant regeneration. When 10 uM tryptophan was added to the germination medium, we obtained the highest yield of protoplasts ($3{\times}10^6 cells/g$) and the best viability (92%). Thirty percent of root protoplast derived from meristematic cells underwent cell division within 5 days in callus-induction medium. Regenerated rosette leaves (2-3 mm) were transferred to rooting medium and finally acclimated to the soil for flowering.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.48 no.1
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• pp.52-60
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• 1999

This paper deals with a fault detection and isolation scheme for a DC motor driven centrifugal pump system. The emphasis is placed on the design and implementation of the residual generatorm, based on parity relation, that provides decision logic unit with residuals that will be further processed to detect and isolate three important faults in the system;brush fault, impeller fault, and the speed sensor fault. Two process faults are modelled as multiplicative type faults, while the sensor fault as an additive one. With multiplicative fault, the implementation of the residual generator needs the time varying transformation matrix that must be computed on-line. Typical implementation methods lack in generality because only a numerical approximation around the assumed fault levels is employed. In this paper, a new implementation method using well tranined neural network is proposed to improve the generality of the residual generator. Application results show that the fault detection and isolation scheme with the proposed residual generator effectively isolates three major faults in the centrifugal pump system even with a wide range of fault magnitude.

• Earthquakes and Structures
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• v.13 no.2
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• pp.119-130
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• 2017

This paper presents a numerical investigation on the seismic behavior of isolated bridges with supplemental viscous damping. Usually very large displacements make seismic isolation an unfeasible solution due to boundary conditions, especially in case of existing bridges or high risk seismic regions. First, a suggested optimal design procedure is introduced, then seismic performance of three real bridges with different isolation systems and damping levels is investigated. Each bridge is studied in four different configurations: simply supported (SSB), isolated with 10% damping (IB), isolated with 30% damping (LRB) and isolated with optimal supplemental damping ratio (IDB). Two of the case studies are investigated under spectrum compatible far-field ground motions, while the third one is subjected to near-fault strong motions. With respect to different design strategies proposed by other authors, results of the analysis demonstrated that an isolated bridge equipped with HDLRBs and a total equivalent damping ratio of 70% represents a very effective design solution. Thanks to confirmed effective performance in terms of base shear mitigation and displacement reduction under both far field and near fault ground motions, as well as for both simply supported and continuous bridges, the suggested control system provides robustness and reliability in terms of seismic performance also resulting cost effective.

• International journal of steel structures
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• v.18 no.5
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• pp.1741-1753
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• 2018

Metallic dampers are sacrificial devices (fuses) that dissipate significant energy during earthquakes while protecting other parts of structures from possible damage. In addition to numerous implementation opportunities of other base isolation systems, U-shaped dampers (UD) are one of the widely investigated and used devices in practice especially in Japan. The present study focuses on enhancing seismic performance of these types of dampers by changing their geometric properties. UDs with perforated (i.e. with holes) and/or nonparallel arms are developed for this purpose. For a better comparison, the criterion of equal material volume (or mass) has been utilized. Three dimensional finite element models of the new type of UDs are formed and investigated numerically under selected displacement histories. Based on the obtained hysteretic curves; dissipated energy intensities, effective stiffness ratios, reaction forces, effective damping ratios are evaluated in this parametric study. It is found that both damper types have merits in use of seismic applications and that the selection of the damper configuration is dependent on the design specific issues.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.6
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• pp.449-452
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• 2001

Legume seed isoflavones may have a variety of desirable physiological effect on the human health including both the circulatory and skeletal systems. The present study was performed to determine the isoflavone content of leaf and seed as well as to purify and identify the types of isoflavone from leaf extract of hyacinth bean (Lablab purpureus (L.) Sweet). Reverse phase HPLC revealed six different types of isoflavone such as daidzin, genistin, daidzein, genistein, 6"-o-acetyl genistin and 6"-o-acetyl daidzin in aqueous methanol extract from seeds and leaves of the hyacinth bean. Relatively, leaf isoflavone content of hyacinth bean was greater than seed isoflavone content. Using DiAion HP-20 silica gel and sephadex LH-20 chromatography, pure daidzein was identified in the ether layer, whereas genistin was in the EtOAC fraction.

• Journal of the Korea institute for structural maintenance and inspection
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• v.12 no.1
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• pp.127-134
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• 2008

For more districted seismic design and attemped multi-bridge continuity, the existing seismic design is difficulted to treat seismic activity. So, many company applied multi-fixed point and damper or isolator, which is effective for decreasing seismic energy, on period shift, decentralization and damping. But, there is hard to design special bridge with adjusted seismic system because of absence seismic device and insufficient design experience. Therefore, the study on behavior characteristics of designed bridge with various seismic device is performed to utilize the result of this for selection of adequate seismic device.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.13 no.5
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• pp.375-383
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• 2003

For a performance analysis of vibration isolation systems, the concept of vibration power flow can be employed preferably when noise radiated from the supporting structure with finite impedances is of interest. The idea is basically simple to understand and formulas for precise estimation of the vibration power are easy to derive. However, It is often required to simplify the process of experimentation under several assumptions due to instrumental limitations. For an example, rotational degree of freedom has not been well treated in bending vibrations of beam or plate-like structures. Yet, several recent studies showed that the moments and rotations play an important role in power transmission and should be taken into consideration carefully as the frequency range of interest goes to audibly high. Therefore, it is readily agreed that reduction of the noise radiation over the high frequency range can be effectively accomplished by adjusting the rotational stiffness of the isolator without changing the vibration isolator efficiency in low frequency range relevant to the translational stiffness of the isolator In this paper, the vibration power flow approach is applied to an AC motor installed on a finite plate in order to illustrate the contribution of the rotational vibration power to the total vibration power transmission. The effects of rotational stiffness of the isolator on the vibration power transmission are investigated by inserting various shapes of Isolators with different rotational stiffness but with $ame translational stiffness between the motor and the plate. The resultant noise radiation from the plate is presented to verify the proposed approach.

• Fisheries and Aquatic Sciences
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• v.19 no.4
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• pp.22.1-22.7
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• 2016

One of the main compounds in Ishige okamurae, diphlorethohydroxycarmalol (DPHC), is known to exhibit antiviral and anti-inflammatory effects. However, it has not been investigated extensively. In this study, preparative centrifugal partition chromatography (CPC) coupled with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ($ABTS^+$) online HPLC was employed for effectively separating considerable amounts of antioxidant compounds from marine algae. Two main antioxidant compounds, DPHC and octaphlorethol A (OPA), respectively, were confirmed and isolated from the ethyl acetate (EtOAc) fraction of I. okamurae by $ABTS^+$ online HPLC and preparative CPC systems. The presence of DPHC and OPA was confirmed in the EtOAc fraction of I. okamurae by both liquid chromatography with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI/MS) and $ABTS^+$ online HPLC systems: DPHC (39 mg) and OPA (23 mg) were successfully isolated from I. okamurae (500 mg) with optimum solvent composition (0.5:10:4:6; n-hexane/EtOAc/MeOH/water, v/v) with corresponding partition coefficients (K) of 1.62 and 2.71, respectively, by preparative CPC. Hence, CPC coupled with $ABTS^+$ online HPLC is convenient for the efficient and simple isolation of these antioxidant compounds from I. okamurae.

• Aerospace Engineering and Technology
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• v.3 no.1
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• pp.179-187
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• 2004

We consider some problems of the Modified SPRT(Sequential Probability Ratio Test) method for fault detection and isolation of inertial redundant sensor systems and propose an Advanced SPRT method which solves the problems of the Modified SPRT method. The problems of the Modified SPRT method to apply to inertial sensor system come from the effect of inertial sensor errors and the correlation of parity vector components. We use a two-stage Kalman filter to remove effects of the inertial sensor errors and propose the modified parity vector and the controlled parity vector which reduces the effect of correlation of parity vector components. The Advanced SPRT method is derived form the modified parity vector and the controlled party vector. Some simulation results are presented to show the usefulness of the Advanced SPRT method to redundant inertial sensor systems.

• Structural Engineering and Mechanics
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• v.91 no.5
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• pp.469-486
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• 2024

Electric transformers are major components of electrical systems, and damage to them caused by earthquakes can result in significant financial loss. The current study modeled a three-dimensional (3D) isolated electrical transformer under horizontal and vertical records from different earthquakes. Instead of using fixed coefficients, an improved wavelet method has been used to create the greatest compatibility between the response spectra and the target spectrum. This method has primarily been used for dynamic analysis of isolated structures with spring-damper devices because it has shown greater accuracy in predicting the response of such structures. The effect of the nonlinear soil-structure interaction on the probability of transformer failure also has been investigated. Soil and structure interaction modeling was carried out using a beam on a nonlinear Winkler foundation. The effect of the nonlinear soil-structure interaction during dynamic analysis of transformers revealed that the greatest increase in the probability of transformer failure was in the fixed-base condition when the structure was located on soft soil. This intensified the response of the structure and increased the probability of transformer failure by up to 27% for far-field and up to 95% for near-field ground motions. A comparison of the results indicates that the use of 3D isolation systems in transformers in areas with soft clay that are subject to near-field ground motions can strongly reduce the probability of failure and improve the seismic performance of the transformer.