• 한국식품과학회지
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• 제2권2호
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• pp.49-55
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• 1970

대두단백육(textured soybean protein food)의 제조 과정에서 분리한 대두단백의 건조공정은 단백 gel의 성질과 보수력, 점성도 같은 단백질 특성에 영향을 미쳤다. 모델시스템의 실험에서 질소용해계수로 표시한 대두단백의 변성도는 분리대두단백 gel의 강도의 중요한 변수로 나타났다. gel strength는 질소용해계수가 43이었을 때 최대가 되었고 질소 용해계수가 그보다 증가할 때나 감소할 때는 gel strength가 감소되었다. 이러한 사실은 분리대두단백의 건조공정 중 적당한 단백의 변성은 단백육제조에 필요하며 그 이상의 높은 질소용해계수를 갖도록 할 필요는 없다.

• Journal of Nutrition and Health
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• 제16권2호
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• pp.81-88
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• 1983

This study was performed to investigate the influence of the amino acid composition of diet and environment on RNA, protein content in brain and learning ability in rats. Forty-two Sprague-Dawley male rats were divided into six groups according to type of diet, casein, soybean meal, or corn gluten and rearing condition, isolated or enriched. They were fed foods ad libitum for 6 weeks. A water maze was used to test behavioral performance for 3 weeks from 4th week. The rats were sacrificed at 6th week and their whole brains were taken and frozen for assay of RNA and protein. The results were summarized as follows : 1) The body weight gain for the experimental periods of corn gluten group was significantly lower than the casein and the soybean meal group. 2) The brain weight of the corn gluten group was significantly lower than the casein and the soybean meal group and the environmental enrichment slightly increased it among rats fed the corn gluten diet. 3) The total RNA contents were the greatest in the environmentally enriched casein group. The brain protein contents of the isolated corngluten group was the smallest. However, the contents of the enriched corn gluten group were similar to those of the others. 4) In the water maze test, the isolated corngluten group spent significantly more time than the others. Environmental enrichment could decrease time to perform the task of the maze.

• Food Science and Biotechnology
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• 제18권2호
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• pp.442-448
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• 2009

In this study Bacillus subtilis PTCC 1023 was used for the production of protopectinase using soybean based media. The use of isolated soybean protein (ISP) and soybean flour resulted in similar protopectinase production and growth rates. The effect of medium composition on protopectinase production was studied using central composite design (CCD) methodology. The change in the concentration of ISP (1-7%), glucose (0-10%), and phosphate (0.1-0.3 M) was found to affect the protopectinase activity (response variable) after 24 hr of cultivation. In the range studied, ISP and glucose had a negative effect on the response variable, whereas phosphate had a positive effect. A statistically significant interaction was identified between phosphate and ISP, suggesting that correct optimization of medium formulation in this case can only be obtained using factorial design of experiments. Protopectinase activity exceeding 215 U/mL was obtained in a medium containing 4% ISP, 0.3M phosphate, and no added sugar.

• 한국식품과학회지
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• 제24권4호
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• pp.311-314
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• 1992

역삼투 방법을 이용한 두부순물의 정화시 역삼투 잔사의 활용 가능성을 검토하기 위해 두부순물 농축물의 성분 특성 및 분리 단백질의 기능성을 조사하였다. 두부순물의 역삼투 잔사 농축물에는 자당, 라피노오스 및 스타치오스가 건물 중으로 각각 32.59%, 4.76% 및 9.99% 함유되어 있었고, 무기물중 칼륨이 5.23%로 회분의 42.7%를 점하였다. 또한 조단백질 함량은 18.69%이었으며 아미노산 조성 및 전기영동상의 sub-unit 양상은 분리 콩단백질과 다소 차이를 보였다. 한편 두부순물로부터 분리한 단백질의 기능성중 pH에 따른 용해도는 분리 콩단백질에 비해 전반적으로 다소 높은 값을, 유화능과 유화안정성 및 점도는 분리 콩단백질보다 비교적 낮은 값을 나타냈다.

• 한국식품영양과학회지
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• 제26권6호
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• pp.1128-1134
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• 1997

Lactobacillus helveticus was inoculated to the fermentation liquid containing skin milk powder(SMT) plus soymilk, SMP plus isolated soybean protein(ISP), SMP plus ISP plus whey powder(WP) to increase the nutritional and economic value of commercial soy yogurt. The yogurt fermented with soymilk and SMP showed the lower acid production than of SMP and had significant beany flavor in the product. The yogurt prepared with ISP and SMP showed the higher cell number and lower acid production than that of SMP. Also, the partial substitution of SMP with ISP over 6%(w/w) produced less acceptable product due to gel production. The yogurt prepared by the partial substitution of SMP with ISP, WP and SMP showed the higher cell number and lower acid production than that of SMP and not bring about gel formation unlike the case of ISP. Sensory properties of yogurt substituted SMP with ISP and WP(38:62 mixture) below 4% were not significantly different from that of SMP and the sample containing the mixture over 6% and 0.067% artificial flavor showed lower sensory score due to beany taste than that of SMP. But increase of yogurt flavor up to 0.1% resulted in significantly high score in organoleptic acceptability. The separation of water occured in yogurt prepared by the combined mixture of ISP, WP and SMP, and this problem could be resolved by addition of Na-alginate and PGA at the concentration of 0.1%(w/w).

• 한국식품영양학회지
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• 제22권4호
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• pp.633-638
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• 2009

In this study, pork meat oligopeptides and ISP oligopeptides were prepared from purified meat protein and, isolated soybean protein, respectively. These oligopeptides were added to porridge. Then their manufacturing suitability and quality characteristics were evaluated. The porridge which included meat oligopeptides and ISP oligopeptides satisfied the 20% RI (recommended intake) of protein and 40% RI of EAA for man between the ages of 20 to 29. According to measurements of the physicochemical characteristics of porridge, the degree of viscosity, spreadability, pH, and lightness L value, were acceptable for consumption. In addition, the oligopeptide powders had good solubility. and were easy to add when cooking. The above results indicate that pork meat oligopeptides and ISP oligopeptides are excellent dietary nitrogen sources for a variety of applications.

• 한국식품영양과학회지
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• 제22권5호
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• pp.643-649
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• 1993

대두의 조성은 다른 두류나 곡류와는 달리 35~40%의 단백질, 15~20%의 유지 및 20~25%의 당을 함유하고 있다. 예로부터 대두는 두부, 간장, 된장과 같은 전통식품의 원료로써 넓게 쓰여져왔다. 초원심분리에 의한 대두 단백의 성분은 침강정수에 의해 2, 7, 11 및 15S의 4가지 주요한 성분으로 나타난다. 저당잔백질 중 중요한 2성분인 7S 및 11S globulin은 많은 연구자들에게 의해 분리되고, 특성이 알려졌다. 미생물 효소에 의해 제조된 응고물은 금속이온 및 산처리에 의해 만들어진 것보다 훨씬 조밀한 구조를 나타내었다. 효소작용으로 얻어진 curd는 식품재료로써 개발되어지고, 식품가공분야에서도 폭넓게 이용될 것으로 기대된다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.283-307
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• 1987

Glycinin and $\beta$-conglycinin are the most abundant storage protein in soybean. These proteins are known to be synthesized predominantly during germination and cell expansion phase of seed development for short period, and synthesized not in other tissues. Genes encoding these storage proteins are useful system to study the mechanism of development stage and tissue specific gene expression in eukaryotes, especially plants, at the molecular level. The cDNA and genomic clones coding for glycinin have been isolated and regulation mechanism of the gene expression has been studied. Initially, development and tissue-specific expression of the glycinin gene is regulated at the level of transcription. Post-transcriptional processing is also responsible for delayed accumulation of the mRNA. Translational control of the storage protein gene has not been reported. Post-translational modification is another strategic point to regulate the expression of the gene. It is possible to identify positive and/or negative reguratory clements in vivo by producing transgenic plants agter gene manipulation. Elucidation of activation and repression mechanism of soybean storage protein genes will contribute to the understanding of the other plant and eukaryotic genes at molecular level.

• 한국식품영양과학회지
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• 제23권6호
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• pp.973-979
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• 1994

Microorganisms, including the strain IJ-3 isolated from soil, were found to secrete an extracellular soybean protein coagulating enzyme and the strain, IJ-3 was identified as Genus Bacillus according to the Bergey's manual . The enzyme coagulated protein in soymilk , thus forming a curd at pHs 5.8-6.4 and at 55-75℃. The optimum temperature for soybean protein coagulating activity was 65-75℃ and the enzyme was stable at temperature below 50℃ and was found to be stable with about 60-100% of the original activity at a with pH ranges(pH6-7). The molecular weight of enzyme was estimated to be 28,000 by SDS-PAGE. The curd formed with the enzyme from Bacilus sp. IJ-3 has a smooth texture, and a mild taste without any bitterness or a beany flavor.

• 한국식품과학회지
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• 제21권6호
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• pp.780-787
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• 1989

쌀과 분리대두단백 혼합에 따른 조직화 특성에서 쌀 혼합량 30%까지 증가할수록 토출구온도, 수용성 질소지수 및 조직잔사지수가 떨어지며 씹힘성과 검성이 감하여 부드러운 조직감이 형성되었다. 또한 쌀혼합량의 증가에 따라 최종 제품의 수분함량은 증가되었으나 밀도에는 변화가 없었고 재흡수력은 감소하는 경향이었다. 한편 복원 후에는 제품의 명도가 밝아졌다. 압출성형 준의 압력분포는 $15{\sim}100kg/cm^3$의 범위였다. 미세구조에서는 쌀혼합량이 증가할수록 기공의 크기가 증대하였으며 쌀 전분질이 호화된 표면구조를 지니고 있었다.