• Title/Summary/Keyword: isogenic lines

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Cooking and Milling Characteristics of Several Barley Starch Isogenic Lines (보리의 전분 Isogenic line들의 취반 및 제분 특성)

  • 송현숙;이홍석;정태영
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.1
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    • pp.63-72
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    • 1994
  • The relationships among the endosperm structure, physicochemical characteristics and cooking and milling properties were examined in this study by using the isogenic lines which have the same genetic background except starch characteristics. The isogenic lines were bread by combining three pairs of genes, of waxy or non-waxy, fractured or round starch granule, and shrunken or plump endosperm. Although grains weight and chemical compositions of the endosperms did not differ widely, but cooking qualities, amylose contents, $\beta$-glucan viscosities were significant differences between isogenic types. Water absorptions and expansibilities were highest in waxy lines, and lowest in fratured starch granular lines; the smaller the seed sizes were, the higher the water absorptions were. Mixogram pattern of cooked barley varied with the starch properties and milling properties were excellent in fractured granular lines, whereas those properties of the waxy and shrunken endosperm lines were not good.

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Comparison among Traits Related to Heading of Barley Near-isogenic Lines for Growth Habit (보리 파성 Near-isogenic Line 간의 출수 및 주요 형질비교)

  • Chun, Jong-Un;Park, Moon-Woong;Lee, Eun-Sup
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.1
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    • pp.16-22
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    • 1990
  • Four near-isogenic lines of growth habit derived from the breeders' seeds of Oweolbori and Jogangbori were obtained, respectively. From the analysis of pedigree charts, the genes for the pertinent growth habit in Oweolbori and Jogangbori seemed to be derived from the Milyang 6 and Hiproly, or Joshushirohadaka, respectively. The spring habit grades of the isogenic lines were classified: Oweolbori #1 and #2, Jogangbori #1 and #2 as I : Oweolbori #3 and #4, Jogangbori #3 and #4 as III, respectively. The spring habit of Oweolbori #1 and #2 might be controlled by one pair of recessive gene, shsh. The photoperiodic response, earliness in a narrow sense, and ratio of heading retardation significantly different in the isogenic lines with the same genetic backgrounds. The heading mature time differed in the isogenic lines sown in fall.

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The Physico-Chemical Properties and Cooking Qualities of Barley Isogenic Lines (보리 Isogenic Lines의 이화학적 품질과 취반특성)

  • Lee, Min-Jae;Kwon, Kyoung-Soohn;Chang, Hak-Gil
    • Applied Biological Chemistry
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    • v.40 no.4
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    • pp.301-306
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    • 1997
  • This study is presented to investigate the physico-chemical properties and qualities for the starch isogenic lines bred in barley(Hordeum vulgare L.).The pearling yield showed higher value in Franubet and Wafranubet than others, but the whiteness of pearled barley varied with the cultivars. The milling rate showed excellence in Franubet, fractured granular lines, whereas that of the waxy and round endosperm isogenic lines is poor. Franubet, the lines having fractures starch granule, was highest in starch content. The highest value in swelling power and water-binding capacity of the barley was proved by the lines having waxy and fractured starch endosperms. The amylogram patterns indicated that the waxy lines such as Wanubet and Wafranubet have lower initial gelatinization temperature and higher maximum viscosity than those of the normal lines. ${\beta}-Glucan\;viscosity$ was generally higher in waxy barley but changed greatly with the cultivars. The water absorption, soluble solid and expansibility in cooking property showed the highest value in waxy and fractured starch granular lines, and the whiteness of cooked barley did not differ with cultivars.

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Development of Near Isogenic Lines and Genetic Analysis for Alkali Digestibility of Rice Grain

  • Kim, Kwang-Ho;Kim, Doo-Hwan;Lee, Jeong-Heui
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.1
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    • pp.12-16
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    • 2001
  • In order to develop near isogenic lines (NILs) the heterozygous rice plants for alkali digestibility value(ADV) were selected and tested in every generation from $F_5$to $F_9$ of a cross, Seratus malam/Suwon 345. Finally several sets of NILs, which were six low ADV lines and four medium-high ADV lines, were selected among $F_{10}$ lines. No differences of the plant growth characteristics, amylose content and protein content of rice grain were found between low and medium-high ADV lines. Rice flour of low ADV lines showed longer gel length in gel consistency test than medium-high ADV lines, and also showed different gelatinization characteristics measured by rapid visco analyzer compared with medium-high ADV lines. The result of genetic analysis indicated that ADV-NILs developed were differed in a pair of major gene controlling ADV, and low ADV was dominant over medium-high ADV.

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The Gene Effect of Culm Length and Ear Length Using Isogenic Lines in Barley (동질유전자계통을 이용한 보리의 간장 및 수장유전자효과)

  • Cho, Chang-Hwan;Lee, Eun-Sup;Sung, Yeol-Kyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.3
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    • pp.270-280
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    • 1988
  • This experiment was conducted to evaluate the main effects and pleiotropic effects of two comparable isogenic lines for culm and spike length in hulled barley and observed various agronomic characteristics, yield and yield components under the conditions of heavy fertilizer application and high planting densities. Three isogenic lines, culm, spike and culm and spike, were planted and to obtain basic data for improving high-yielding and quality of hulled barley at Experiment Farm, Dankook Univ., Cheonan city in 1985. Differences of culm length between two comparable isogenic lines, short culmed line (SCL) and medium culmed line (MCL), in isogenic lines for culm length showed highly significant differences. And differences of spike length between two comparable isogenic lines, short spike line (SSL) and medium spike line (MSL), in isogenic lines for spike length showed highly significant differences. Differences of culm and spike length between two comparable isogenic lines, short culmed and spike line (SCSSL) and medium culmed and spike line (MCMSL), short culmed and spike line (SCSSL) and long culmed and spike line (LCLSL), in isogenic lines for culm and spike length showed highly significant differences. Characteristics affected highly by the genes of culm length in isogenic lines for culm length were heading days, the 1st and 2nd internode length, No. of grains per spike, No. of spikes/m$^2$, and one litre weight. Characteristics affected highly by the genes of spike length in isogenic lines for spike length were No. of spike nodes, heading days, maturity days, thousand grain weight and one litre weight. Characteristics affected highly by the genes of culm and spike length in isogenic lines for culm and spike length were No. of spike nodes, the 2nd, 3rd and 4th internode length, thousand grain weight and grain yield. Grain yield of SCL, MSL, MCMSL and SCSSL was more than grain yield of MCL, SSL, SCSSL and LCLSL, respectively. Maturity days of SCL, MSL, SCSSL and LSLSL were faster than maturity days of MCL, SSL, MCMSL and SCSSL, respectively.

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Change of Agronomic Traits of Tillering Isogenic Lines under Different Seedling Density in Barley (보리 분얼성 동질유전자계통의 재식밀도 조절에 따른 특성 변화)

  • 천종은
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.1
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    • pp.34-44
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    • 1996
  • Several major agronomic traits of 4 isogenic lines for tillering(Kindred, Morex)were investigated under the different seeding rates and seedling mortality grown in the pots. The normal-tillering phenotypes had higher grain yield(46%) and leaf area index(56%) than their uniculm isogenic counterparts. The assortment rate(69%) and 1,000 grain weight(27%) in uniculm type were greater than the normal type. Uniculm plants were earlier in heading(4∼8days) and maturing(2∼3 days) dates compared with their counterparts. The grain yield was fitted to linear regression, ranged 0.659g to 0.5g per spike number. On increasing the seedling mortality rates, the grains per spike and 1,000 grain weight were slightly decreased, but the grain yield per plot was decreased markedly because of decrease of spikes per plot. The assortment rate, leaf photosynthetic capacity and reproductive tiller number were increased with increase of seedling mortality. The grain yield was largely dependent on the number of spikes per plot on occurrence of seedling mortality. For the uniculm genotypes to be introduced and bred into a present leading variety, the isogenic lines were precisely tested in the open field for a couple of years.

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Effects of Hulled and Hulless Barley Isogenic Lines on Germination and Emergence Rate (보리 피과성이 발아 및 출아율에 미치는 영향)

  • Lee, Eun-Sup;Jeong, Duk-Hyun;Chun, Jong-Eun;Nam, Jung-Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.1
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    • pp.78-83
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    • 1986
  • To obtain the information about the reasons why hulless barley varieties generally have poor germination and emergence rate in the field, hulled and hulless isogenic lines were bred and used. The germination rates of hulless isogenic lines were 4-6 percent lower than those of hulled lines at artificial precipitation treatment, and seriously dropped down at 5 day precipitation treatment. As the thresher rotation speeds up 600 to 1,000 rpm, the differences of germination rate were 10 percent in hulled, but 22 percent in hulless lines. Also emergence rates of hulless lines became seriously low at deep seeding. The emergence rates of hulless isogenic lines became low in the order of deep seeding, high speed thresher, and rain-fall. These results suggest that barley breeders especially for hulless varieties should plan their breeding programs to improve the emergence rate or seedling vigour in the field.

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Comparisons of Growth and Yield Characters between Near-isogenic Lines with Dark and Pale Green Leaves in Rice (수도 농녹색엽과 담녹색엽 Near-isogenic 계통의 생장특성 및 수량형질 비교)

  • Park, Sun-Zik;Im, Byeung-Gi;Lee, Byun-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.2
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    • pp.226-230
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    • 1986
  • Two near-isogenic lines with dark and pale green leaves, derived from the F$\_$7/ generation of wxl26 cross were compared on photosynthetic capacity, growth parameters, grain yield and yield-related characters. Dark green-leaved lines contained much greater content of chlorophyll a and b than pale green-leaved ones, but chlorophyll a to b ratio showed no difference between them. The photosynthetic nte per unit leaf area was higher in dark green-leaved lines than in pale green-leaved ones in the flag leaves at heading stage, but that per unit chlorophyll content showed reversed result. The crop growth rate from transplanting to heading was consistantly higher in the dark green-leaved lines, resulting from their greater net assimilation rate. Dark green-leaved lines produced greater number of panicles and spikelets per hill, out yielding pale green-leaved lines, but ripened grain ratio and 1000-grain weight showed no differences between those lines.

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Breeding of Near Isogenic Lines of Silkworm (Bombyx mori L.)

  • Li, Muwang;Xu, Anying;Hou, Chengxiang;Zhang, Yuehua;Huang, Junting;Guo, Xijie
    • International Journal of Industrial Entomology and Biomaterials
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    • v.6 no.2
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    • pp.207-210
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    • 2003
  • Four different backcrossing methods were designed and 23 near isogenic lines (NILs) of 22 linkage groups were obtained using Hb as recurrent parent, the mutant gene lines which held markers as donor parents. Eleven of them had been mated with the recurrent parent for 10 times, and the others for 7∼8 times. The NILs of other 6 linkage groups are under way and had been backcrossed to the recurrent for 3∼4 times. These NILs will act important roles in the construction of molecular linkage map and gene location and positional cloning.