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Isolation and Taxonomical Characterization of Streptomyces sp. JR-24 with Antibacterial Activity of Bacterial Leaf Spot of Pepper (Xanthomonas axonopodis pv. vesicatoria) (고추 세균성 점무늬병원균(Xanthomonas axonopodis pv. vesicatoria)의 항균활성 Streptomyces sp. JR-24 균주의 분리 및 분류학적 특성)

  • Han, Song-Ih;Lee, Hyo-Jin;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.46 no.4
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    • pp.359-365
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    • 2010

  • Fifty Actinobacteria strains were isolated from rhizosphere soil of Sasa borealis. In the course of screening for antibacterial activity against bacterial leaf spot of pepper (Xanthomonas axonopodis pv. vesicatoria) of isolates, 12 isolates showed strong antibiotic activity. Basis on the 16S rRNA gene sequence, they were belonging to Streptomyces cluster II. Strain JR-24 exhibited strong antibiotic activity against X. axonopodis pv. vesicatoria, had a minimum inhibitory concentration of 10 ${\mu}l$/disc. The strain JR-24 was most closely related to Streptomyces galbus $DSM40089^T$ (98.1%), Streptomyces longwoodensis $LMG20096^T$ (98%) and Streptomyces capoamus $JCM4734^T$ (97.8%). When assayed with the API 20NE and 50 CHE kit, it is positive for utilization of L-arabinose, D-fructose, D-glucose, D-galactose and hydrolysis of gelatin, protein, starch. The strains contained iso-$C_{14:0}$ (25.93%), iso-$C_{15:0}$ (10.13%), anteiso-$C_{15:0}$ (19.29%) and iso-$C_{16:0}$ (20.35%) as major fatty acids and MK-9 (H4), MK-9 (H6), and MK-9 (H8) as the isoprenoid quinone. Strain JR-24 was suggested new species of genus Streptomyces by nearest neighbors of genotypic relationships and phenotypic characterization. This study was important to microbial resources investigation for environment-friendly agriculture.

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Evaluation on Expectation of Deflection of Floor Damping Materials Subjected to Long-Term Load (장기하중을 받는 바닥완충재의 처짐 예측 평가)

  • Kim, Jung-Min;Hong, Yoon-Ki;Kim, Jin-Koo;Lee, Jung-Yoon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.20 no.4
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    • pp.19-26
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    • 2016

  • Floor damping materials used in floating floor system to diminish the floor noise have been made with low density and dynamic stiffness. Owing to this low density and dynamic stiffness, the deflection in these materials under long-term loading and cracking of the floor finishing mortar in the floating floor system may occur. This paper presents the results of long-term loading effects on the deflection of different types of floor damping materials. The experimental program involved the long-term loading tests for 490 days loading period on sixteen specimens. Specimens were classified as DM1(Damping Materials) to DM8, depending upon the four main parameters; types, bottom shapes and densities of floor damping materials and amount of loading. Results indicated that the long-term deflection of all specimens of damping materials remained unchanged after 200 days at all loading amounts, except the specimens made up of Polystrene, in which long-term deflection remained unchanged after 160 days at 250 N load and 100 days 500 N load. In this paper, two types of correlation expressions were shown in the deflection range prior to the range where deflection remained constant; two analyses by ISO 20392 and linear regression. In comparison of two analyses and experimental results on the difference of deflection of 16 specimens, the difference of deflection was below 0.4 mm in those analyses in case of that total deflection was below 10 mm. Restrictively, it was judged that the analysis for the deflection of specimens made up of Polystrene is more appropriate using ISO 20392.

  • https://doi.org/10.11112/jksmi.2016.20.4.019 인용 PDF KSCI