• Restorative Dentistry and Endodontics
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• v.20 no.2
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• pp.549-576
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• 1995

Bacteria are one of the most important causative agents of the pulpal and periapical diseases. Streptococci are one of the most frequently isolated facultative anarerobic bacteria in the infected root canals. Bacterial cell wall components have a direct effect in the pathogenesis of the pulpal and periapical infections. Hyaluronidase produced by bacteria has been implicated in dissemination of the diseases. The purpose of this study was to evaluate the effect of cell wall extract of streptococci on the L929 cells using inverted microscope and the transmission electron microscopy (TEM). Hyaluronidase production of streptococcal strains were investigated to determine the correlation between the severity of cell damage and the activity of enzymes. Bacterial cell wall extracts of S. sanguis, S. mitis and S. uberis isolated from infected root canals and ATCC type strains of S. mutans (ATCC 10449) and E. faecalis (ATCC 19433) were prepared by sonication and confirmed with SDS-PAGE. Silver stain of SDS-PAGE of sonic extract was efficient at $100{\mu}g$/ml concentration of cell wall protein, while Coomasie blue stain was efficient at $100{\mu}g$/ml concentration. Inverted microscope showed that sonic extract-treated L929 cells were round and detached from the substratum while others lost their fibroblastic shapes. Transmission electron microscopic examination revealed that streptococcal extracts induced death of L929 cells. Sonic extracts of streptococci had variable effect on microstructure of L929 cells. significant chromatin condensation was observed in the nucleus of the cells. Disappearance of cell surface microvilli and nuclear fragments with dense chromatin were observed. The cell nucleus had an irregular shape and numerous large vacuoles were seen in the cytoplasm and some breaks of the cell membrane could be seen. Cell organelles were in various stages of destruction and cristae of mitochondria were disoriented or disappeared. Eighteen strains of streptococci did not produce hyaluronidase.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.6
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• pp.1277-1285
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• 2015

It has been known that the conventional retaining wall system with timber lagging and H pile has several problems such as the irregular gap between H-piles, cutting or adding to standard timber, back fill over first step excavation, and especially break-down accident at the disjoint of wall system. In the practical excavation, these problems may lead to worker's accident and the inefficiency of construction economy. To solve the above problems, a new method using prefabricated retaining wall was proposed and verified. The characteristics of the new method is to replace timber wall as free-sliding steel-lagging and connector. To check its verification and application, laboratory tests such as bending strength, tensile strength, and fatigue strength were carried out. Also, a pilot test in the field and numerical simulations under various ground conditions were performed. From the researches, it is found that the prefabricated retaining wall plate can be superior to the conventional timber lagging plate in the strength. It is also found that the proposed methods can be effective in the reuse of retaining wall plate and safe in the disjoint of wall system. Finally, it is desired that the proposed method will be effective in the reduction of the imported timbers and helpful in the safety of retaining wall construction.

• Journal of the Society of Naval Architects of Korea
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• v.38 no.2
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• pp.1-9
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• 2001

Based on the linear potential theory with the side wall reflection. the directional spectrum waves are numerically simulated by a source distribution method and these together with long-crested irregular waves are also generated at the towing tank of Pusan National University by considering the transfer function of the wave maker obtained from the regular waves. In the numerical simulation, the characteristics of the directional spreading function are investigated by changing the breadth of the wave-maker unit. the width of the towing tank and the wave period. In the experimental generation, the statistical properties and the power spectrums of the long-crested irregular and directional waves are compared along the towing tank length. The directional spreading functions are also investigated at various positions in the tank.

• Journal of Veterinary Clinics
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• v.35 no.4
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• pp.146-149
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• 2018

A 7-year-old, castrated, male Maltese dog presented with hyporexia and depression for 3 days. Elevated serum amylase, lipase activities, and liver enzyme values were found upon blood examination. An abdominal mass was seen on radiographs caudal to the gastric body in the left middle abdomen. In the left middle abdomen, abdominal ultrasonography also revealed a massive, irregularly marginated, heterogeneous mass of unknown origin, and in the right cranial abdomen, heterogeneously hypoechoic pancreatic tissue and hyperechoic change of adjacent mesenteric fat were observed. Contrast-enhanced computed tomography showed an irregular contour of the left pancreatic limb as well as heterogeneously enhanced parenchyma. A low-attenuating peripancreatic fluid collection with a thin and irregular wall was also seen. Based on these findings, an atypical pancreatic abscess with necrotizing pancreatitis which manifested as walled-off necrosis was suspected. The mass was excised, and the pancreatic abscess was confirmed by histopathologic examination. No complications were found in the patient after two months of follow-up examination.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.6
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• pp.814-821
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• 2000

The Discrete Ordinates Interpolation Method (DOIM) is tested in three-dimensional enclosures. The radiative transfer equation (RTE) is solved for a linear source term and the DOIM is formulated for a gray medium. Several interpolation methods can be applied to the DOIM scheme. Among them, the interpolation method applicable to an unstructured grid system is discussed. In a regular hexahedron enclosure, radiative wall heat fluxes are calculated and compared with exact solutions. The enclosure has an absorbing, emitting and nonscattering medium and a constant temperature distribution. These results are obtained with varying optical depths (xD = 0.1, 1.0, 10.0). Also, the same calculations are performed in an irregular hexahedron enclosure. The DOIM is applied to an unstructured grid system as well as a structured grid system for the same regular hexahedron enclosure. They are compared with the exact solutions and the computational efficiencies are discussed. When compared with the analytic solutions, results of the DOIM are in good agreement for three-dimensional enclosures. Furthermore, the DOIM can be easily applied to the unstructured grid system, which proves the reliability and versatility of the DOIM.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2021.05a
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• pp.6-7
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• 2021

3D printing has the unique advantage of the ability to customize freeform products even in small quantities. It was recently applied successfully in the node connection system for the irregular shaped glass design of Gwanggyo Galleria Department Store. This was achieved by applying 3D printing technology for an innovative smart node design. The novel system offers flexibility to address various design challenges in addition to maximizing construction quality and reducing construction period. In this paper, we aim to examine the use of 3D printing based innovative technologies in the construction industry. With this aim in mind, we present the case of the Gwanggyo Galleria Department Store's smart nodes. The key objective of this study is creating awareness in the construction industry of the need to utilize fourth industrial revolution technology in architecture.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.13 no.4
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• pp.263-272
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• 2001

Recently, some attempts to construct slit caisson-type breakwaters are made in Korea. Since slit caisson-type breakwaters are suitable for relatively deep sea areas, a lot of theoretical and experimental researches have been performed. In this study, the reflection characteristics of vertical slit caisson breakwaters are investigated based on the measured data in two-dimensional hydraulic model tests with irregular waves. The experiments were conducted for various cases; variation of porosity of perforated-wall, width of wave chamber, number of slits for single-and double-chamber, respectively. It is found that in the case when the wave steepness (H/L$_{s}$ ) is small, the reflection coefficients are large. The existing researches have shown that the wave reflection is minimized when the nondimensional width of wave chamber B/L is about 0.2~0.25 for the regular waves. However, for the irregular waves the reflection is lowest when $B/L_2$, is 0.13~0.15. For a same porosity condition, the wave dissipation is stronger as the width of s1it is larger. The double-chamber caisson is superior to single- chamber caisson in the wave dissipating effects.

• Proceedings of the KWS Conference
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• 2002.10a
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• pp.639-643
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• 2002

The main purpose of this study was to investigate the formation mechanisms of imperfections such as irregular humps, outer cavity and inner cavity in the laser fusion zone of diamond saw blade. Laser beam welding was conducted to join two parts of blade; mild steel shank and Fe-Co-Ni sintered tip. The variables were beam power and travel speed. The microstructure and elements distributions of specimens were analyzed with SEM, AES, EPMA and so on. It was found that these imperfections were responded to heat input. Irregular humps were reduced in 10.4∼17.6kJ/m heat input range. However there were no clear evidences, which could explain the relations between humps formation and heat input. The number of outer cavity and inner cavity decreased as heat input was increased. Considering both possible defects formations mechanisms, it could be thought that outer cavity was caused by insufficient refill of keyhole, which was from rapid solidification of molten metal and fast molten metal flow to the rear keyhole wall at low heat input. More inner cavities were found near the interface of the fusion zone and sintered segment and in the bottom of the fusion zone. Inner cavity was mainly formed in the upper fusion zone at high heat input whereas was in the bottom at low heat input. Inner cavity was from trapping of coarsened preexist pores in the sintered tip and metal vapor due to rapid solidification of molten metal before the bubbles escaped.

• Journal of Ocean Engineering and Technology
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• v.21 no.1 s.74
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• pp.7-17
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• 2007

Based on the Boussinesq wave model, the wave distribution in the Chagui-Do sea area in Jeju was simulated by applying the directional irregular waves at an incident boundary. The time and spatial variations of monthly mean wave height and period were investigated, which aims to provide basic information on optimal sites for wave power generation. The grid size and time interval of the Boussinesq wave model were validated by examining wave distributions around a surface piercing wall, fixed at sea bottom with a constant slope. Except for the summer season, the significant wave height is dominated by wind waves and appears to be relatively high at the north sea of Chagui-Do, which is open to the ocean, while it is remarkably reduced at the rear sea of Chagui-Do because of its blocking effect on incident waves. In the summer, the significant wave height is higher at the south sea, and it is dominated by the swell waves, which is contributed by the strong south-west wind. The magnitude of significant wave height is the largest in the winter and the lowest in the spring. Annual average of the significant wave height is distinctively high at the west sea close to the Chagui-Do coast, due to a steep variation of water depth and corresponding wave focusing effect. The seasonal and spatial distribution of the wave period around Chagui-Do sea reveals very similar characteristics to the significant wave height. It is suggested that the west sea close to the Chagui-Do coast is the mast promising site for wave power generation.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.20 no.1
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• pp.41-49
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• 1990

The author observed the changes of vasculature of pre-and post-irradiation on DMBA induced rat tongue cancer. The study was performed by using vascular corrosion resin casting, and scanning electron microscopy. The results were as follows. 1. The capillaries runned parallely and formed bundles and, sometimes, plexus. The endothelial cells were arranged regularly and small pores were observed. 2. In irradiated normal tongue the capillaries were curved slightly and formed plexus on initial day of post-irradiation. On third day the capillaries and capillary pores were dilated and the endothelial cell arrangement was irregular. The effects of irradiation were gradually increased from initial to the 3rd day, though it was decreased after 7th day. 3. The vasculature of DMBA induced tongue cancer group were very irregular, and large avascular lesions were formed according to the cancer necrosis or tumor cell nest and the vasculature was narrowed and paralleled around the avascular lesion by compression of cancer cell nest. The vascular wall was roughened and dilated, forming club shaped or varix. 4. The vessels were curved and formed reticular network in irradiated DMBA induced tongue carinoma group. The free end of newly formed capillaries had regular width, and also irregular club shaped or aneurysmal dilatation were observed. The vascular structures were destroyed and vessels were fused in tumor necrosis lesion. The radiation effects were marked on the first and third day of irradiation and the effects were decreased after seventh day and showed capillary regeneration.