• Journal of Microbiology and Biotechnology
• /
• 제20권7호
• /
• pp.1156-1162
• /
• 2010

During ethanol fermentation, bacterial strains may encounter various stresses, such as ethanol and acid shock, which adversely affect cell viability and the production of ethanol. Therefore, ethanologenic strains that tolerate abiotic stresses are highly desirable. Bacteria of the genus Deinococcus are extremely resistant to ionizing radiation, ultraviolet light, and desiccation, and therefore constitute an important pool of extreme resistance genes. The irrE gene encodes a general switch responsible for the extreme radioresistance of D. radiodurans. Here, we present evidence that IrrE, acting as a global regulator, confers high stress tolerance to a Zymomonas mobilis strain. Expression of the gene protected Z. mobilis cells against ethanol, acid, osmotic, and thermal shocks. It also markedly improved cell viability, the expression levels and enzyme activities of pyruvate decarboxylase and alcohol dehydrogenase, and the production of ethanol under both ethanol and acid stresses. These data suggest that irrE is a potentially promising gene for improving the abiotic stress tolerance of ethanologenic bacterial strains.

• Journal of Microbiology and Biotechnology
• /
• 제25권12호
• /
• pp.2125-2134
• /
• 2015

IrrE is a highly conserved global regulator in the Deinococcus genus and contributes to survival from high doses of UV radiation, ionizing radiation, and desiccation. Drad-IrrE and Dgob-IrrE from Deinococcus radiodurans and Deinococcus gobiensis I-0 each share 66% sequence identity. However, Dgob-IrrE showed a stronger protection phenotype against UV radiation than Drad-IrrE in the D. radiodurans irrE-deletion mutant (ΔirrE), which may be due to amino acid residues differences around the DNA-binding HTH domain. Site-directed mutagenesis was used to generate a Drad-IrrE A184S single mutant, which has been characterized and compared with the ΔirrE mutant complemented strain with Drad-irrE, designated ΔirrE-E. The effects of the A184S mutation following UV radiation and mitomycin C (MMC) shock were determined. The A184S mutant displayed significantly increased resistance to UV radiation and MMC shock. The corresponding A184 site in Dgob-IrrE was inversely mutated, generating the S131A mutant, which exhibited a loss of resistance against UV radiation, MMC shock, and desiccation. qPCR analysis revealed that critical genes in the DNA repair system, such as recA, pprA, uvrA, and ddrB, were remarkably induced after UV radiation and MMC shock in the ΔirrE-IE and A184S mutants. These data suggested that A184S improves the ability against UV radiation and MMC shock, providing new insights into the modification of IrrE. We speculated that the serine residue may determine the efficiency of DNA binding, leading to the increased expression of IrrE-dependent genes important for protection against DNA damage.

• 수산해양기술연구
• /
• 제17권2호
• /
• pp.109-113
• /
• 1981

Most existing theories on ship waves and wave resistance are based on the perturbation of the flow field by a small pararr.eter which specifies the slenderness of the ship hull. Since however, ship hulls in practice are neither so slender nor thin enough to secure the validity of the linearized theory, the agreen:ent between the theoretical prediction and the experimental result is not generally satisfactory. The author pointed out that the contribution by the non-linear term in the free surface condition can be represented by sorr.e source distribution over the still water plane. This paper leads to a forrr.ula for the wave resistance of not slender ships at low Froude nurr.bers. and deals with the asynptotic expression. As a nurr.erical example, the wave resistance of Wigley model is calculated, and the result is compared with experimental values. It is concluded that the wave resistance coefficient varies in the rate of Fn6 at low speed limit in general. A comparison with the result derived from the linearized free surface condition shows that the non-linearity of the free surface is irr portant at low speed.