• Biomedical Science Letters
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• v.19 no.2
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• pp.164-167
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• 2013

Osteoclasts are originated from hemopoietic progenitors of the monocyte/macrophage lineage and resorb mineralized tissues. Elevated osteoclast numbers and activity result in bone disease such as osteoporosis, Paget's disease, and tumor osteolysis. In order to identify the genes that are involved in osteoclast differentiation, microarray was performed after treated with RANKL for 12 h and 24 h in osteoclast precursors. The genes that changed by RANKL treatment were grouped by biological process or molecular function. Among them, the number of genes involved in signal transduction and nucleic acid binding was 6065 and 3066, respectively. When analyzed the number of genes changed more than 1.5 fold in the cells treated with RANKL for 12 h or 24 h compared to when RANKL was not treated, 83 and 62 genes were up-regulated; 56 and 62 genes were downregulated, respectively. To verify the microarray results, real-time RT-PCR for Cxcl1 and Slfn1genes that have not been reported yet related to osteoclast differentiation, as well as Ccl2 gene associated with osteoclast differentiation were carried out. Both experiments showed a similar result of more than 1.5 fold induction of these genes by RANKL treatment. These results suggest the possibility that Cxcl1 and Slfn1 may associate with osteoclastogenesis and provide that microarray is a useful tool to analyze the profile of genes changed during osteoclast differentiation by RANKL. Moreover, this gene profile contributes to understand the regulatory mechanisms involved in osteoclast differentiation and the pathogenesis, thus developing therapeutics of bone diseases such as osteoporosis.

• International Journal of Oral Biology
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• v.37 no.4
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• pp.167-173
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• 2012

This study investigated the genes involved in the differentiation of odontoblasts derived from human dental pulp stem cells (hDPSCs). hDPSCs isolated from human tooth pulp were validated by fluorescence activated cell sorting (FACS). After odontogenic induction, hDPSCs were analyzed investigated by Alizaline red-S staining, ALP assay, ALP staining and RT-PCR. Differential display-polymerase chain reaction (DD-PCR) was performed to screen differentially expressed genes involved in the differentiation of hDPSCs. By FACS analysis, the stem cell markers CD24 and CD44 were found to be highly expressed in hDPSCs. When hDPSCs were treated with agents such as ${\beta}$-glycerophosphate (${\beta}$-GP) and ascorbic acid (AA), nodule formation was exhibited within six weeks. The ALP activity of hDPSCs was found to elevate over time, with a detectable up-regulation at 14 days after odontogenic induction. RT-PCR analysis revealed that dentin sialophosphoprotein (DSPP) and osteocalcin (OC) expression had increased in a time-dependent manner in the induction culture. Through the use of DD-PCR, several genes were differentially detected following the odontogenic induction. These results suggest that these genes may possibly be linked to a variety of cellular process during odontogenesis. Furthermore, the characterization of these regulated genes during odontogenic induction will likely provide valuable new insights into the functions of odontoblasts.

• Journal of the Korean Operations Research and Management Science Society
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• v.28 no.2
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• pp.17-33
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• 2003

The explosive growth of the internet-based transactions requires not only a secure payment system but also an appropriate trust measuring methodology and secure transaction protocols to guarantee the minimal risk for the transacting entities involved in specific transactions. Especially, in internet auction systems where either buyers or sellers or both can be more than one in one transaction, providing those systems that make sure no one transacting entity takes a major risk becomes critical. In this paper, an improved trust measuring method using a relationship-based internet community for an auction system is proposed. The proposed system incorporates fuzzy set and calculation concepts to help build trust matrices and models, which is used to measure the level of risk involved in a specific auction trade concerned. Also, to optimize the auction trade process in terms of cost and time, the proposed system recommends a differentiated trade protocol according to the risk level involved in each auction trade. To test the appropriateness of the proposed trusted auction system, a prototype system has been developed under a Windows-NT environment.

• Development and Reproduction
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• v.21 no.4
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• pp.467-473
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• 2017

Ascidian embryos have become an important model for embryological studies, offering a simple example for mechanisms of cytoplasmic components segregation. It is a well-known example that the asymmetric segregation of mitochondria into muscle lineage cells occurs during ascidian embryogenesis. However, it is still unclear which signaling pathway is involved in this process. To obtain molecular markers for studying mechanisms involved in the asymmetric distribution of mitochondria, we have produced monoclonal antibodies, Mito-1, Mito-2 and Mito-3, that specifically recognize mitochondria-rich cytoplasm in cells of the ascidian Halocynthia roretzi embryos. These antibodies stained cytoplasm like reticular structure in epidermis cells, except for nuclei, at the early tailbud stage. Similar pattern was observed in vital staining of mitochondria with DiOC2, a fluorescent probe of mitochondria. Immunostaining with these antibodies showed that mitochondria are evenly distributed in the animal hemisphere blastomeres at cleavage stages, whereas not in the vegetal hemisphere blastomeres. Mitochondria were transferred to the presumptive muscle and nerve cord lineage cells of the marginal zone in the vegetal hemisphere more than to the presumptive mesenchyme, notochord and endoderm lineage of the central zone. Therefore, it is suggested that these antibodies will be useful markers for studying mechanisms involved in the polarized distribution of mitochondria during ascidian embryogenesis.

• Journal of the Korea Safety Management & Science
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• v.10 no.4
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• pp.173-179
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• 2008

The portion of air logistics is getting growing in the global logistics and customer need the rapidity and accuracy for freight delivery. But, the process of air logistics is so complicated because there are too many stakeholders involved in the process. So we suggest the way to make simple the process for improving ability of handling air freights. First, we analyze the AS-IS process and problems by studying previous documents and researching the field. Also we offer how to adopt the ways that are solutions concerning AS-IS process. Second, we suggest integrated information system that will make it simple the air logistics process and mitigate the intensive work load of air cargo. Also we show the reliance about our suggestion by simulation comparing TO-BE process to AS-IS process. We result in that this study will contribute to improve air logistics process by making simple the process.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2000.10a
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• pp.37-41
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• 2000

Extruded Profiles of Aluminum alloys have been widely used as parts and frames in mechanical and construction structures. Nowadays, mechanical processing of extruded Al alloy profiles is often employed for various industrial applications. Especially, the bending process is more and more applied and the process is greatly influenced by the distributed mechanical properties in the extruded profiles. Due to large reduction of area or extrusion ratio in ordinary production of extruded profiles, anisotropy is naturally induced by large severe deformation during the extrusion process. Therefore, the anisotropy properties play a great role in the bending process, as a post processing of extruded profiles and errors will be involved when the extruded profiles are treated as isotropic material, ignoring the induced anisotropy in the thin-walled extruded product. In the present work, the anisotropic material change is simulated, as a simplified method, employing Barlats six-component yield criterion in the rigid-plastic finite element method. Finite element computations are carried out for extrusion of a thin-walled part.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2000.11a
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• pp.595-599
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• 2000

This paper describes an Axiomatic Design Process enhanced by the Combinative Index that represents combinative strength between function requirements and design parameters. This method combines the advantages of these two methods : 1) Combinative Index that represents combinative strength between function requirements and design parameters so that we clearly understand these information. 2) engineering specifications are categorized into strategies, constraints and Functional Requirements. In this paper, relationship of FR's and DP's is regarded as one in which uncertainty of information are fundamentally involved. In the reduction of problem with uncertainty, we propose an enhanced Axiomatic Design Process using Combinative Index.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1995.03a
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• pp.224-232
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• 1995

A process is designed by the backward tracing scheme of the rigid-plastic FEm for net-shape shell nosing component without machining after forming. The current process of the shell nosing industry requires cost-consuming machining to produce final product . The backward tracing scheme of the rigid-plastic FEM, a novel method in preform design of metal forming processes , derives a sound preform for net-shape shell nosing product . The current process is simulated to check the metal flow involved informing with a trial preform and its modified preform. It is found that the two preforms are not suitable for net-shape shell nosing product. Finally, a preform is desinged by the backward tracing scheme, which is suitable for net-shape manufacturing of the shell nosing component.

• Journal of the Korean Ceramic Society
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• v.47 no.4
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• pp.338-342
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• 2010

Co, $Fe_3O_4$ and Co/$Fe_3O_4$ nanoparticles were synthesized by a polyol process in order to develop their new applications and improve chemical, magnetic properties. The synthesis involved a polyol process using Fe, Co acetylacetonate as precursors and 1-2 hexadecanediol as the polyol. The synthesized $Fe_3O_4$ and Co/$Fe_3O_4$ nanocomposite particles were monodispersed and self arrayed ranging in size of 8~10 and 10~25 nm, respectively. The Co nanoparticle has a crystallite size of 10~40 nm. The synthesized nanoparticles were characterized by their structural, morphological, compositional and magnetic properties using TEM-EDS, XRD, and PPMS techniques.

• Industrial Engineering and Management Systems
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• v.10 no.3
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• pp.191-196
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• 2011

The purpose of this paper is to study user's involvement in new product development (NPD). It seeks to identify the factors involving user and design practices in the design process of new product development. A survey was conducted on 20 respondents consisting of designers involved in product development from various industries. The study focused on the early activities of the product design process which is called product specification. The analysis performed considers the importance of involving users in design decision. The outcome of this research is the significance of involving users and its effect on product development activities. The research also provides a model for an integrated user, designer and product knowledge activity in the product development process.