• 생태와환경
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• 제35권3호통권99호
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• pp.220-231
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• 2002

2000년 4월부터 2001년 9월까지 남강댐 하류의 본류와 유입하천의 어류상 및 군집구조를 조사하였다. 조사기간 중 채집된 어류는 11과 30속 39종이었으며, 그 중 잉어과 어류가 58.9%, 기름종개과 어류가 12.8%로 높은 값을 나타내었다. 채집된 종에서 한국 고유종은 R. uyekii, A. koreensis, A. yamatsutae, C. leuciscus, S. chankensis tsuchigae, S. gracilis majimae, S. variegatus wakiyae, M. yaluensis, M. rapidus, k. nakdongensis, N. multifasciata, L. mediadiposalis, C. herzi, O. platycephala의 14종이었다. 본류에서만 채집된 종은 P. esocinus, S. scherzeri, P. altivelis, C. argus의 4종이었으며, 지류에서만 채집된 종은 R. ocellatus, C. splendidus, S. gracilis majimae, H. longirostris, M. yaluensis, M. rapidus, Z. temmincki, M. mizolepis, K. nakdongensis, N. multifasciata, P. fulvidraco, L. mediadiposalis, C. herzi, O. platycephala의 14종이었다. 본류의 다양도　지수는 0.979로 지류의 다양도　지수 0.966보다 약간 높은 값을 나타내었다. 본류의 St.3과 지류의 St. 1, 16은 조사지역 중에서도 각각 높은 다양도 지수 (1.05, 0.962,0.956)와 낮은 우점도(0.102,0.369,0.200)를 보여 안정된 군집구조를 가지고 있는 것으로 나타났다. 국외 및 국내의 다른 수계에서 도입된 종은 M. salmoides, L. macrochirus, O. bidens와 P. fulvidraco의 4종이었다.

• 한국환경복원기술학회지
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• 제13권3호
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• pp.23-35
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• 2010

A Sustainable Structured wetland Biotop (SSB) system was constructed on the floodplain of Kyungan stream in December, 2006. It purifies polluted water of Keumeo stream which flows into the stream. Water were sampled once a month at inlet and outlet from December, 2007 to December, 2008. $BOD_5$, SS, T-N and T-P were analyzed. Plant and fish species of the system were monitored twice during the period. Average influent and effluent BOD5 concentration was 6.2 and 2.2 mg/L, respectively and BOD5 removal was 50.8%. SS concentration of influent and effluent was averaged 10.1mg/L and 1.5mg/L, respectively and SS abatement amounted to 77.0%. Average influent and effluent T-N concentration was 4.9mg/L and 2.9 mg/L, respectively and T-N retention was 50.8%. T-P concentration of influent and effluent was averaged 0.386mg/L and 0.107mg/L, respectively and T-P removal amounted to 77.0%. Twenty two plant species were naturally introduced into the system, however, they didn't make up a significant portion of the plant populations compared with the planted species. Dominant plant species were in the following order; Phragmites communis > Typha latifolia > Iris pseudoacorus > Persicaria thunbergii. Five families and 15 species of fish were observed in the system including Chinese minnow (Moroco oxycephalus) which inhabits in clean water. Six more fish species were monitored in the system compared with ones living in Kyungan stream. Amphibia and reptiles accounted for 11 species of 4 orders and 7 families including Korean Salamander (Hynobius leechi) which also lives in cleanwater.

• 한국어병학회지
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• 제23권1호
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• pp.1-8
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• 2010

무지개 송어 발안란의 이동 이력과 IHNV G gene 염기서열의 계통학적 분석에 의해 IHNV의 국내 이동에 대해서 처음으로 조사되었다. IHNV RtWanju09 분리주는 IHNV RtPy91과 RtJe00의 JRt Shizuoka lineage에 속하는 것으로 나타났으며, 강원도에서 분리된 IHNV RtPy91과 RtWanju09 사이의 G gene의 유전적 차이는 1.77%였고, 약 20년간 우리나라의 JRt Shizuoka lineage에 속하는 한국 분리주들은 최대 3.03%의 유전적 차이를 나타내 계속적으로 바이러스가 진화하는 것을 알 수 있었다. 또한 전라북도 지역에서 분리된 IHNV RtWanju09 분리주는 IHNV가 오염된 강원도 지역산 발안란이 전라북도에 전파되었을 것으로 사료된다.

• 한국어병학회지
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• 제15권2호
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• pp.49-56
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• 2002

Effects of Temperature on the Pharmacokinetics of norfloxecin (NFX) were studied in the cultured carp,Cyprinus carpio, and cel, Anguilla japonica, using high performance liquid chromatography (HPLC) originally developed for quinolone determination in livesrocks. Pharmacokinetics of NFX was apparently affected by ambiem water temperature. In a two-compaament model for carp after oral dosage of 20 mg/K01 at $13^{\circ}C$ and $23^{\circ}C$ and 5.20/hr, respectively. In carp the $K_{\iota\nu}$, $T_{max}$and $C_{max}$ for carp at $13^{\circ}C$ were 13.30/hr, 17.44 ${\mu}g$/$m\ell$ and 7.00 ${\mu}g$/$m\ell$, respectively. The" correspoeding values at $23^{\circ}C$ were 3.93/hr, 15.40 ${\mu}g$/$m\ell$ and 9.44 ${\mu}g$/$m\ell$, respectively. The AUC and T were 355.66 ${\mu}g$ hr/$m\ell$, and 12.70 hr at $13^{\circ}C$ and 417,24 ${\mu}g$ hr/$m\ell$ and 13.86 hrs at $23^{\circ}C$, respectively. Similar trends were revealed in the NFX pharmacokinetics of eel kept under the two water temperature regimes aftee oral NFX dosage of 20 mg/kg. These pharmacokinetkal results have some implication in the optimal usage of recently introduced antibacterials in farmed fish, which were originally adapted for poultry and mammalian species.

• 한국가축번식학회지
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• 제25권3호
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• pp.277-286
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• 2001

기존의 미세주입 및 정자 electroporation에 의한 보다 효율적인 유전자 도입방법을 개선하여 간단하고 고효율성의 유전자 변환기술을 위한 유전자 도입장치의 시제품 개발로 다수의 난자를 전기적으로 단순화할 수 있는 상업화의 가능성을 보여주었다. 도입된 유전자는 모든 초기배에서 발현됨을 보여 주었다. 특히 난황내의 합포체세포(syncytium)에서의 transient성의 강한 발현은 전기자극에 의해 많은 수의 난자에 유전자를 도입하고 100% 발현되는 체계를 이용하여 transient 시기에서 인간 유용단백질 생산의 가능성을 타진할 수 있는 결과를 보여 주었다. 어류유전자 발현의 작동되는가를 검색하기 위해 신경세로조직특이 tubulin promoter 를 이용한 결과 gfp의 발현이 뇌주변과 척추를 중심으로 체내 전반의 신경세포내에 발현이 강하게 나타남을 보여 주었다. 한편 reporter 유전자 이외에 간세포로부터 전체 RNA를 분리시켜 vitellogenin의 분해산물인 phosvitin cDNA의 길이와 promoter지역인 1.6 kb에 대한 primer쌍들을 선정한 상태에서 PCR에 의해 각각 cDNA와 gDNA로부터 cloning 중에 있으며 human factor Ⅶ과 epidermal growth factor, vitellogenin의 3종의 target 단백 질유전자를 구축 및 검정 화인 중에 있다.

• 한국임상수의학회지
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• 제29권1호
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• pp.58-62
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• 2012

To protect aquatic animal health of importing countries from the potential risks associated with exotic diseases introduced through international trade of live aquatic animals, inspection of designated commodities at ports of entry is a critical component of the safeguarding system. The only way to be 100% confident that no fishes in a shipment are infected with a specific agent is to test every fish in the commodity imported with a perfect diagnostic test. For the majority of cases, this is unrealistic since the group of interest may very large particularly for aquatic animals, or imperfect tests are often available. It is, therefore, more common to test a fixed proportion of a group by preplanned sampling schemes. However, decision making based on results of testing the sample can provide quite a chance that infected groups may be misclassified as uninfected, depending on sampling strategy employed. The objective of this study was to determine the possibility that one or more fishes in the group imported being infected but tests negative after inspecting samples. This question is critical to government authorities to examine whether sampling plan is sufficient to achieve the purpose intended for. At fixed population size, the maximum number of infected fishes when all tests negative was decreased as the sampling fraction increased. The probability of including at least one undetected but infected fish in a group for negative tests increased with the number of fish tested or true prevalence. The risk was much lesser where high sensitivity test was assumed; when increasing test sensitivity from 0.9 to 0.99, this risk was dramatically reduced to about a tenth or a fourth for prevalence ranges from 2 to 10%, given sample size ranges from 10 to 200. Based on the preliminary analysis, the author concluded that current sampling plan testing 4-8% of the import proposal for human consumption still can yield high false negative results. Therefore, from the quarantine inspection point of view, an enforced commodity-specific sampling design that accounts for the cost of testing with an imperfect test at the specified design prevalence is urgent.

• 한국어류학회지
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• 제20권1호
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• pp.36-44
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• 2008

2006년부터 2007년까지 옥정호에 도입된 외래어종 배스 Micropterus salmoides를 채집하여 식성을 조사하였다. 채집된 배스의 체장범위는 13~511 mm (n= 419)였다. 배스는 강한 육식성 어종으로 주로 어류를 섭식하였으며 그밖에 수서곤충과 새우류 등을 섭식하였다. 배스는 성장하면서 식성이 변하였는데, 10~20mm는 주로 지각류를 섭식하였고, 30~50 mm은 수서곤충과 소형어류를, 50 mm 이상의 개체는 피라미, 치리, 밀어와 같은 어류를 주로 섭식하였다. 1981년부터 옥정호의 어류상을 분석한 결과, 강한 육식성 어종인 배스의 도입은 옥정호 토착종의 다양도와 출현비율의 감소를 초래하는 것으로 판단된다.

• 수산경영론집
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• 제36권1호
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• pp.139-153
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• 2005

Marine fish aquaculture has been introduced on the East sea since 1964. The production volume of the marine aquaculture has been increasing drastically since 1990 with the technological improvements and the introduction of new species. However, the increasing costs of feed, seedlings and medical supplies and the decreasing prices due to the increase in the imports and domestic production have decreased the profits in the aquaculture business. Futhermore, the damage from the natural disasters such as red tides and typhoons has accelerated the exit from the business. Even though managing abilities to overcome the business crisis caused by the above factors are required, business managers based on the small scale family businesses do not have enough managing abilities to deal with the difficulties in the business. On the other hand, advanced countries in the marine aquaculture such as Norway have reached the stage where the managing abilities of marine aquaculture are similar to those in the manufacturing industry. And the number of large scale aquaculture farms with developed technologies and advanced marketing strategies in those countries is increasing. Considering that the marine aquaculture in Japan under the similar fishery systems of Korea has developed the state-of-the-art management skills or lead to large scale management, it is difficult to expect the decrease in the production costs under the small scale family business in Korea and this will lead to the decreasing competitive advantage over the imported seafood. Therefore marine aquaculture in Korea needs to increase the economy of scale to acquire the competitive advantage. This study addresses the possibility of introducing the large scale aquaculture system in Korea by analysing the factors and process of leading to large scale businesses in the aquaculture in Japan.

• 한국식생활문화학회지
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• 제23권4호
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• pp.438-447
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• 2008

"Gyuhapchongseo" was published in 1809, and introduced the cooking method of the noble class in the late Joseon dynasty. The characteristics of the side dishes in "Gyuhapchongseo" are as follows. Firstly, red pepper was used as whole red pepper, shredded red pepper, powdered red pepper, or Korean hot pepper paste. Secondly, salt-fermented fish was used in some forms of Kimchi, including Sukbakgi, Dong A Sukbakgi, and Gyochimhae. Thirdly, to retain the juiciness of meat during roasting, meat was spread cold water on the surface, dipped into the washing water of rice or wrapped with wet paper. Fourth, to improve the visual effect of a dish, cooked foods were displayed with various color schemes, panfried foods with two tones (egg white and yellow) of color on each side and the use of radish pigmented with deep red color. On examination of the characteristics of food in "Gyuhapchongseo", I would suggest applicable practices for the present cuisine. The use of gravy produced from the boiling down of fish flesh could raise the nutritive value of Kimchi. In "Gyuhapchongseo", Yak po (semi-dried minced beef) is noted as being good for elderly people with bad teeth. A steamed dish with dog meat in Dong A in "Gyuhapchongseo" is made by hollowing out Dong A and putting a dog in it to cook the dog meat to well done in a fire made with the hulls of rice. This technique could be used to present cuisine for steamed and roasted dishes using food ingredients such as pumpkin, sweet pumpkin, and overripe cucumber.

• 현장농수산연구지
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• 제25권3호
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• pp.35-46
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• 2023

It was in the 1982 that artificial seed production research for olive flounder (Paralichthys olivaceus) farming was first conducted in Korea (Currently, National Institute of Fisheries Science, Fish Breeding Research Center). In 1985, fertilized eggs were obtained from natural olive flounder adapted to land tanks, and artificial seed production technology was established and fertilized eggs were distributed. In the late 1980s, halibut aquaculture began to prosper in land-based tank farming in Jeju Island and Busan's Gijang region, where water temperatures are relatively high in winter. Currently, aquaculture is being carried out all over the country, centering on Jeju Island and Wando, Jeollanam-do. However, olive flounder farming, which started with a small group in the 1980s, reduced genetic diversity through inbreeding over generations, resulting in side effects such as slow growth, reduced resistance to disease and environmental conditions. In order to solve these genetic problems of farmed olive flounder in Korea, the Fish Breeding Research Center of the National Institute of Fisheries Science introduced a wild-caught parent fish group to the existing aquaculture group from 2003 to 2004. Genetic diversity was secured and KingNupchi with fast growth and improved body shape was developed. In this study, the current status of breeding technology development of olive flounder, a major aquaculture breed in Korea, is reviewed and future research directions are suggested.