• Korean Journal of Environment and Ecology
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• v.18 no.3
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• pp.326-332
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• 2004

A study of freshwater ichthyofauna was conducted in order to clarify its significance at 14 upstream sites in Muju, Jangsu, Geochang and Hamyang districts in Deogyusan National Park, enclosing Baekdudaegan. The freshwater fish fauna of the Park was measured at the 14 sampling locations from May 2003 to September 2003. The fifteen species gauged included four Korean endemic species and or subspecies (Squalidus japonicus coreanus, Iksookimia longicorpa, Coreoperca herzi, Odontobutis platycephala). Fourteen species were found in the Keum River and seven species in the Nakdong River. However, common species were Punchocypris, Rhynchocypris oxycephalus, Zacco temmincki, Misgurnus anguillicaudatus, Coreoperca herzi, and Odontobutis pzatycephala. The dominant species of these regions were Zacco temmincki and Rhynchocypris oxycephalus. Past studies conducted in Baekdudaegan mostly concernedflora and vegetation. In the future, new study concepts for stream corridors involving both animal and plant should be introduced.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.5
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• pp.433-441
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• 1995

A series of experiments was conducted to determine the influence of various pepsin-HCL pretreatment factor, hereby the factors of duration of washing for the retrieved bags, inherent to the mobile nylon bag technique (MNBT), on apparent ileal digestibility of crude protein (AIDCP) and apparent ileal digestibility of dry matter (AIDDM). At last, the AIDCP and apparent ileal digestibility of amino acids (AIDAA) in maize, barley, wheat, rapeseed meal, cottonseed meal and three mixed diets were determined with the MNBT and ileo-rectal anastomis pigs (IRAT). For the MNBT techniques, bag measuring $25{\times}40$ MM and containing 0.75 g feedstuff samples, after pre-digestion in vitro, were introduced into the ileo-rectal anastomis pigs (IRAT) gastrointestinal tract through a duodenal cannula and recovered in the ileal digesta between 6 and 12 h. later. 1. The apparent ileal digestibility of dry matter (AIDDM) and crude protein (AIDCP) of the tested samples, with the exception of fish meal, determined by MNBT were not affected by the different pepsin-HCL pretreatment times in vitro between 2.5 h. and 4 h. 2. There was no significant (p > 0.05) difference of the AIDCP and AIDDM of maize determined by the MNBT among different pepsin concentration (0.03%, 0.07% and 0.1 %) treatment in vitro. 3. The AIDCP determined with the MNBT was affected by the washed and unwashed recovered bags from the ileal digesta. 4. The AIDCP and AID amino acids (AIDAA) of maize, barley, wheat, rapeseed meal, soya-bean meal, cottonseed meal and three mixed diets from the MNBT, with a solution of 0.01N HCL (PH 2) and 0.1% of pepsin concentration, a pepsin-HCL pretreatment time in vitro or 4h. and a washing time of the recovered bag from the ileal digesta compared well with those from the IRAT. The linear regression analysis showed a significant correlation (p < 0.01) of AIDCP and AIDDA between the IRAT and MNBT.

• The Journal of the Convergence on Culture Technology
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• v.2 no.1
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• pp.1-12
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• 2016

This article examines the types and cooking methods of Emandoo as recorded in 15 royal palace studies and 8 old literature texts of the Joseon dynasty (1392-1909). Early Joseon Dynasty kind of Emandoo one kind, two kinds of mid and late 15 kinds, a total of 18 paper was introduced. A Emandoo ingredient materials of a pheasant, chicken, beef, abalone, sea cucumber, etc. were used. Emandoo were able to see that the current 'mulmandu' form of life that icing on the water after the starch into a small thin sliced fish flesh. The size of a Emandoo made small clams. The use of 'Gocho (苦椒)' on Chojang(醋醬), starting with 'emandoo" of Muhsin jinchan Euigwe"(1848) Further study will be conducted on recipes and ingredients recorded in these Euigwe and old books in order to develop a standardized recipe to make Emandoo appealing to a global palate.

• Journal of Life Science
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• v.27 no.5
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• pp.524-529
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• 2017

This study was aimed to produce a transgenic zebrafish expressing the human KCNE1 gene. Initially, the entire CDS of the human KCNE1 gene was amplified from a human genomic DNA sample by polymerase chain reaction using a primer set engineered with restriction enzyme sites (EcoRI, BamHI) at the 5' end of each primer. The resultant 402 bp KCNE1 amplicon flanked by EcoR1 and BamH1 was obtained and subsequently cloned into a plasmid vector pPB-CMVp-EF1-GreenPuro. The integrity of the cloned CDS sequence was confirmed by DNA sequencing analysis. Next, the recombinant vector containing the human KCNE1 (pPB-CMVp-hKCNE1-EF1-GreenPuro) was introduced into fertilized eggs of zebrafish by microinjection. Successful expression of the recombinant vector in the eggs was confirmed by the expression of the fluorescence protein encoded in the vector. Finally, in order to assure that the stable expression of the human KCNE1 gene occurred in the transgenic animal, RNAs were extracted from the animal and the presence of KCNE1 transcripts was confirmed by RT-PCT as well as DNA sequencing analysis. The study provides a methodology to construct a useful transgenic animal model applicable to the development of diagnostic technologies for gene therapy of LQTS (Long QT Syndrome) as well as tools for cloning of useful genes in fish.

• Fisheries and Aquatic Sciences
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• v.23 no.4
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• pp.10.1-10.10
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• 2020

Background: Butaphosphan (BTP) has recently been introduced into the Korean aquaculture sector as a stressattenuating agent. In this study, a sensitive chemical analytical method was established for the detection of BTP in the olive flounder (Paralichthys olivaceus) tissues. Methods: Utilizing a method employing liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), detection sensitivity, specificity, and precision were satisfactorily established. Temporal changes in the BTP plasma and muscle concentrations were assessed after a single intramuscular injection of BTP (50 and 150 mg/kg) to the olive flounder maintained at 13 ℃ or 22 ℃. Results: High BTP plasma levels were achieved immediately after the injection, and the drug was rapidly eliminated. Additionally, plasma BTP levels were markedly dependent on the elimination rate, which, in turn, seemed dependent on the water temperature, with the drug elimination half-life and mean residence time significantly shorter at 22 ℃ than 13 ℃. Overall, muscle BTP levels were markedly lower than the plasma levels. Notably, muscle levels were not influenced by water temperatures. Muscle BTP concentrations were used to estimate the necessary withdrawal period for drugs used in food fish, with BTP levels maintained far below the possible hazardous limit. Conclusions: In conclusion, the established LC-MS/MS method can be used for BTP residue detection with high sensitivity and reproducibility.

• Korean Journal of Ichthyology
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• v.32 no.3
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• pp.182-190
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• 2020

The endangered Pseudopungtungia tenuicorpa were reintroduced to the Jojongcheon Stream in the Hangang River 2011~2012 as part of the restoration project. Since then, the restoration project has been evaluated as a failure because the P. tenuicorpa were not collected from the discharge site 2014~2016. This study was conducted 2017~2019 to verify that the P. tenuicorpa restoration project failed. Six locations were selected, including the discharge site, and the fish collection method used skimming nets, cast nets, long bag set nets, and scuba diving. In the survey results, 13 individuals were collected in a 0.5 km section, including the discharge station. The P. tenuicorpa habitat was approximately at a water depth of 0.5 m to 2 m in run water flow with the boulders and rocks. The age of the collected P. tenuicorpa were 0+ to 3-years-old, and the generation alternation was occurring stably. Thus, the P. tenuicorpa restoration project was evaluated as partially-successful, as the P. tenuicorpa, re-introduced to the Jojongcheon Stream, settled in its habitat with stable generation alternation.

• The Journal of Fisheries Business Administration
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• v.35 no.2
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• pp.31-51
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• 2004

The reduction of fishing grounds due to the establishment of EEZ system among China, Japan and Korea together with the depletion of fish resources by pollution of waters and successive reclamation projects along the east and the south coastal lines has made many problems in coastal communities including the decrease of population, the deepening aging phenomenon in the fishery society and the fall of relative income level compared with those of rural and urban residents. Especially, the income level of a fishing household is 90%. of a rural resident and 70% of a urban resident. The income of a fishing household consists of 55% of fishing income, 20% of a agricultural source, 20% of income from non-fishing areas, and 10% of transferred income. Compared with that of a Japanese fishing household which has more diverse income sources such as 62% of income coming from non-fishing areas through being hired in manufacturing firms, etc., that of Korean ones is highly dependent upon fishery and agriculture, so that the diversification of income sources is urgently needed, especially in non-fishing areas. This paper shows that as a model to upgrade fisherman's income level, firstly, it is necessary to enhance the value-added of fishing products through processing and new innovation of distribution process and, secondly, to promote tourism in fishing villages. To ascertain this model, a questionnaire survey to fishermen was carried out and showed that they expressed a strong support for the increase of income by the value-added process through processing and innovative distribution system and the active introduction of tourism in fishing villages. A case study on Gosan cooperative in Jeju was also introduced to identify the rationale of the suggested model and this study proved the validity of the model again. Conclusively speaking, to level up the fisherman's income requires the value- added activities through the introduction of product processing and new distribution system together with the introduction of marine tourism in fishing villages.

• Journal of Digital Convergence
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• v.15 no.12
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• pp.543-548
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• 2017

$360^{\circ}$panoramic image can give people an unprecedented visual experience, and there are many different ways to make a $360^{\circ}$panoramic image. In this paper, we will introduce two easy and effective methods from those many ways. The first one is through 48 photos to make a $360^{\circ}$panoramic image, the second way is through 6 photos to make a $360^{\circ}$panoramic image. We will compare those methods and tell the audience which one suits themselves. Through those easy design methods introduced above, we can see VR works design became easy and popular, normal people can also make $360^{\circ}$panoramic image, and it promotes the industry of VR image contents.

• Korean Journal of Construction Engineering and Management
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• v.4 no.3 s.15
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• pp.119-127
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• 2003

Among project delivery methods, Design-Build method is becoming an increasingly preferable alternative to traditional contracting method. The Design-Build delivery method was first introduced in Korea, 1975. But it still has been producing many problems. The previous researches had been suggested several ways to improve the Design-Build project delivery but it seemed to have had no effect whatsoever. This research proposes the success model for Design-Build delivery method through the 4 steps. First, find the proper modeling method. Second, establish the As-Is model for analyzing the circumstances of Design-Build method. Third, to begin with analyzing the As-Is Design-Build model and previous researches, establish the problem model. Finally, find success factors and circumstances through the overseas Design-Build system, and then establish the success Design-Build model.

• Corrosion Science and Technology
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• v.9 no.1
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• pp.48-55
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• 2010

The many vessels are built with FRP(Fiber-Reinforced Plastic) material for small boats and medium vessels. However, FRP is impossible to be used for recyclable material owing to environmental problems and causes large proportion of collision accidents because radar reflection wave is so weak that large vessels could not detect FRP ships during the sailing. Hence, Al alloy comes into the spotlight to solve these kinds of problems as a new-material for next generation instead of FRP. Al alloy ships are getting widely introduced for fish and leisure boats to save fuel consumption due to lightweight. In this study, it was selected 6061-T6 Al alloy which are mainly used for Al-ships and carried out various electrochemical experiment such as potential, anodic/cathodic polarization, Tafel analysis, potentiostatic experiment and surface morphologies observation after potentiostatic experiment for 1200 sec by using the SEM equipment to evaluate optimum corrosion protection potential in sea water. It is concluded that the optimum corrosion protection potential range is -1.4 V ~ -0.7 V(Ag/AgCl) for 6061-T6 Al alloy, in the case of application of ICCP(Impressed current cathodic protection), which was shown the lowest current density at the electrochemical experiment and good specimen surface morphologies after potentiostatic experiment for Al-Mg-Si(6061-T6) Al alloy in seawater environment.