• Korean Journal Plant Pathology
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• v.14 no.2
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• pp.157-163
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• 1998

Genetic diversity among 27 isolates of Rhizoctonia solani, which were obtained from diseased crops in Korea and classified into 9 intraspecific groups by anastomosis test and cultural characteristics, was studied by PCR-RFLP. Gene regions of nuclear 17S ribosomal DNA and internal transcribed spacers including 5.8S rDNA of the isolates were amplified with polymerase chain reaction and digested with 12 restriction enzymes. Differences of restriction patterns were not shown among isolates within each intraspecific groups, however, each anastomosis group and culturala type sowed unique restriction fragment length polymorphisms by restriction patterns using HaeIII, Cfr13I and MspI. The results suggest that PCR-FRLP of rDNA using three restriction enzymes could be used to differentiate intraspecific groups of Rhizoctonia solani in Korea.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.519-525
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• 1998

Intraspecific genetic diversity of Korean isolates of Phytophthora drechsleri was investigated based on PCR-RFLP of rDNA along with closely related species in the genus; P. cryptogea, P. melonis, P. erythroseptica, P. cinnamomi, P. cambivora and P. cactorum. Gene regions of nuclear small subunit and internal transcribed spacer (ITS) in rDNA were amplified with polymerase chain reaction and digested with 9 restriction enzymes. Phytophthora species was readily differentiated from each other based on the digestion patterns, however, P. cryptogea was not separable from some isolates of P. drechsleri. Twenty one isolates of P. drechsleri originated from 15 host plants were divided into three distinct groups designated as PdG1, PdG2 and PdG3, respectively. Four isolates in PdG1 were originated from green vegetables and tomato and nine isolates in PdG2 were mainly isolated from medicinal plants. The two groups showed 95.3% homology and four isolates of P. cyptogea came under the groups. However, Eight isolates in PdG3 collected from cucurbits were clearly differentiated from those of PdG1 and PdG2 by 66.5% homology, but completely matched with a Taiwan isolate of P. melonis. Results indicated that three distinct groups exist in Korean isolates of P. drechleri and each group has host preference. In addition, reclassification of the cucurbits isolates are reserved because of their distinct genetic characters from other intraspecific groups in P. drechsleri.

• Korean Journal of Environmental Biology
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• v.22 no.2
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• pp.295-299
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• 2004

The lacertid lizard Takydromus wolteri widely distributed in South Korea, but intraspecific diversity of this species was not almost studied. T. wolteri represented by 8 specimens collected from 5 localities were analyzed by the Randomly Amplified Polymorphic DNA (RAPD) method with 28 random decamer primers and the phenogram constructed by using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method based on RAPD data. The populations of T. wolteri using in this paper were collected from Gyeonggi-do, Chungcheongbuk-do, Jeju-do, Jeollanam-do and Gyeongsangnam -do in South Korea. Among 68 different bands detected in RAPD analysis, 59 bands (87％) showed polymorphism. The phenogram showed that the populations of T. wolteri were grouped into two. These results suggest that T. wolteri were supported intraspecific diversity.

• Korean Journal of Plant Resources
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• v.13 no.2
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• pp.104-110
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• 2000

To analyse the genetic relationship and intraspecific variations among the Eleutherococcus senticosus population, the polymerase chain reaction(PCR) was performed total genomic DNAs of 10 E. senticosus collections by random 10 primers. The genetic diversity and genetic distance among 10 collections of Eleutherococcus spp. were used to describe the dendrogram showing phylogenic relationship. Ten collections were classfied into two group(group I, II) at the similarity coefficient value of 0.50. Group I included E. senticosus of Bukhado(Japanese), youngwal(Korea), E. seoulense, and E. chiisanesis while group II included several internal and Russia collection. The range of polymorphism was from 66.7 to 90.9% in 87 amplified DNA fragments. The similarity value of all collections ranged from 0.41 to 0.92. The average of genetic distance was 0.61.

• The Plant Pathology Journal
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• v.26 no.1
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• pp.83-89
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• 2010

Taxonomic position of 46 Korean isolates of Rhizoctonia solani which were classified into nine intraspecific groups by anastomosis and cultural characteristics was analyzed by randomly amplified polymorphic DNA (RAPD) and sequence analyses of the internal transcribed spacer (ITS) regions of ribosomal DNA. All the isolates within each group showed highly similar band patterns in RAPD. The ITS regions of the isolates within the same groups showed a high level of sequence similarity above 96.0% whereas similarities among different groups were below 94.4%. When compared with several reference strains of R. solani from foreign countries, all the Korean isolates were clustered with the foreign isolates belonging to the same groups in the phylogenetic tree. All six Korean strains of AG-4 were identified as HG-1 out of 3 subgroup of AG-4. We discussed taxonomic position of Korean isolates of R. solani and showed that sequence analysis with ITS regions could be a rapid and useful method for identification of intraspecific group of R. solani.

• Fisheries and Aquatic Sciences
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• v.21 no.11
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• pp.31.1-31.7
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• 2018

Three economic marine algae that have been used as food and carrageenan sources were collected from Korea during a survey of marine algal flora. They share the generic features of Hypnea, and three major clades supported by the sectional features were confirmed in a phylogenetic tree based on rbcL sequences. The first species, which belongs to a species group corresponding to the sect. Spinuligerae, nests in the same clade with Hypnea yamadae in a genetic distance of 0%. It is morphologically characterized by an entangled base, subcompressed or subterete to terete axes, somewhat percurrent main axis, irregularly alternately branching with wide angle, and rarely hooked spinous branchlets. The second one is also referred to the sect. Spinuligerae and formed the same clade as Hypnea cenomyce. The genetic distance between both sequences was calculated as 0.0-0.1%, which is considered to be intraspecific. This species is distinct by somewhat entangled thallus at the basal part, percurrent axis, short spine-like branchlets densely covering the axis, and medullary lenticular thickenings. The third alga, which forms a species group corresponding to the sect. Pulvinatae, nests in the same clade as Hypnea nidulans (no intraspecific divergence). It shows occasionally epiphytic habitat rather than epilithic habitat of low mat-forming growth and percurrent erect main axes with dense lateral branchlets. Based on these morphological and molecular data, the three Korean species are identified as H. yamadae, H. cenomyce, and H. nidulans. This is the first record of the Hypnea species in Korea.

• Animal cells and systems
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• v.11 no.2
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• pp.255-263
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• 2007

A taxonomic study on the genus Tropocyclops has been accomplished as a part of the series of studies on the freshwater cyclopoid copepods in South Korea. As a result, the Tropocyclops prasinus, hitherto known from Korea in many reports and papers, turns out to be a species complex of three sibling species: T. prasinus (Fischer, 1860), T. ishidai n. sp., and T. setulifer n. sp. This paper deals with description of two of these new species with taxonomic accounts on their inter- or intraspecific morphological discrepancies.

• Journal of Microbiology
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• v.35 no.4
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• pp.259-263
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• 1997

To study the phylogenetic relationships of species of Trichaptum and to infer intraspecific dibergence of T. abietinum, partial mitochondrial small subunit rDNA sequences were determined. Six strains of T. abietinum, two of T. biforme, and one of T. fusco-violaceum were examined. Parsimony and distance analyses showed that each Trichaptum species forms a distinct group and that T. abietinum consists of two or more subgroups. Strains from North America were distantly related to one another but the European strain formed an independent group with three Korean strains, suggesting the possibility that Korean taxa may be phylogenetically closer to European taxa than to North American taxa.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.1
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• pp.72-78
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• 2014

We investigated the molecular phylogeny and genetic differences among local populations of Korean Hemiculter fishes based on their mitochondrial cytochrome b gene sequences. Our results indicated that Hemiculter leucisculus populations in China were clearly divided into two groups. The first group (Group 1) included the populations of the Yangtze River and its surrounding areas (including the Qiantangjiang, Lingjiang, Jiulongjiang, and Minjiang rivers); the second group (Group 2) contained local populations from southern China (including the Nanliujiang, Zhujiang, Wanquanhe, Qianjiang, and Nandujiang rivers). The Korean Hemiculter eigenmanni differed in its cytochrome b gene sequence by 0.6-1.0% from the Chinese H. leucisculus (Group 1), which inhabited the Yangtze River and its surrounding areas, suggesting they were phylogenetically close and likely to be the same species. The Korean H. leucisculus differed from the Chinese H. leucisculus (both Groups 1 and 2) by 8.1-9.5%, indicating a very distant phylogenetic relationship; however, the Korean H. leucisculus differed from Hemiculter bleekeri by only 0.5-0.7%, showing intraspecific nucleotide differences. We conclude that the taxonomic relationship between the Korean H. leucisculus and H. bleekeri requires further investigation using type specimens.

• Animal Systematics, Evolution and Diversity
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• v.21 no.2
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• pp.137-156
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• 2005

A taxonomic study on the Eucyclops serrulatus species group has been accomplished as one of the serial researches on the freshwater cyclopoid copepods in Korea. As a result, the 'Eucyclops serrulatus', hitherto known from Korea through many reports and papers, turns out to be a species complex of six sibling species: E. serrulatus (Fischer), E. roseus Ishida, E speratus (Lilljeborg), E. pacificus Ishida, E. ohtakai Ishida, and I tsushimensis Ishida Taxonomic accounts on their detailed interspecific discrepancies and intraspecific variabilities are presented. A key to the Eucyclops serrulatus group from the Far East is also provided.