• Mycobiology
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• v.48 no.4
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• pp.296-303
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• 2020

Three yeast strains (Hue-1, Hue-8, and Hue-19) with strong heavy metal tolerance were isolated from mangosteen from Hue city, Vietnam. They exhibited identical phenotype and phylogeny. Sequence analysis of the D1/D2 region of the LSU rRNA gene and the internal transcribed spacer (ITS) region demonstrated that the closest relative of these strains is Papiliotrema sp. with 2.12% and 3.55-3.7% divergence in the D1/D2 domain, and ITS domain, respectively. Based on the physiological, biochemical, and molecular data, the three strains belong to a novel species of Papiliotrema genus, for which the name Papiliotrema huenov sp. nov. is proposed. These strains are highly tolerant of heavy metals compared to other yeasts, being able to grow in the presence of 2 mM Pb (II), 2 mM Cd (II), and up to 5 mM Ni (II), but no growth was observed in the presence of 1 mM As (III).

• The Korean Journal of Mycology
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• v.45 no.1
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• pp.63-67
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• 2017

Through a survey of symbiotic endophytic fungi, we isolated an endophyte fungal strain from the roots of Glycyrrhiza uralensis in Korea. The isolated fungal strain was identified using its morphological characteristics and through phylogenetic analysis of the internal transcribed spacer, the large subunit rDNA region, and the translation elongation factor region. The strain was identified as Neocosmospora rubicola. This species has not been previously reported in Korea. In this study, we report its isolation from the roots of Glycyrrhiza uralensis in Korea, followed by the characterization and identification of the strain.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.471-472
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• 2001

Recently many studies show that genetic characters can be used to provide unambiguous taxonomic discrimination when morphological characters are not practical for routine identification to species level (Bucklin et al., 1996, 1998; Lindeque et al., 1999). The internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA are also one of appropriate markers for species-level studies because it contains sufficient diversity to address intra- and interspecific phylogenetic relationships in invertebrates (Odorico and Miller,1997; Schizas et al., 1999). (omitted)

• Mycobiology
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• v.49 no.5
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• pp.498-506
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• 2021

An endophytic fungus strain DYSJ3 was isolated from a stem of Aphanamixis grandifolia Blume, which was identified as Aspergillus versicolor based on the morphological characteristics, internal transcribed spacer (ITS) and calmodulin gene sequences analyses. A. versicolor DYSJ3 exhibited strong antagonistic activity against Colletotrichum musae, C. gloeosporioides and Fusarium oxysporum f. sp. cubense with the inhibition rates of 61.9, 51.2 and 55.3% respectively. The antifungal metabolites mainly existed in the mycelium of A. versicolor DYSJ3, and its mycelial crude extract (CE) had broad-spectrum antifungal activities against plant pathogenic fungi. The CE had a good thermal stability, and the inhibition rate of 100 mg/mL CE against C. musae was above 70.0% after disposing at 120 ℃ for 1 h. Five secondary metabolites were isolated from the CE and identified as averufanin, ergosterol peroxide, versicolorin B, averythrin and sterigmatocystin. Activity evaluation showed versicolorin B exhibited inhibitory effects on the mycelial growth and conidial germination of C. musae, and sterigmatocystin had a weak inhibitory effect on the mycelial growth of C. musae.

• The Korean Journal of Mycology
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• v.51 no.2
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• pp.127-133
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• 2023

We report the first discovery of Penicillium mexicanum in Korea. Fungal strains were isolated from air samples collected in Taean-gun, Chungcheongnam-do, Korea. The strain was identified based on its morphological characteristics, as well as molecular phylogenetic analysis of the internal transcribed spacer (ITS), β-tubulin (BenA), and calmodulin (CaM) regions. This strain exhibited a high sequence similarity to the reference sequences of P. mexicanum. These findings enhance our understanding of fungal biodiversity in Korea and underscore the importance of continuous monitoring of fungal species.

• Mycobiology
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• v.51 no.4
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• pp.210-215
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• 2023

The aim of this study was to characterize a new fungal species, Acremonium conglutinatum, isolated from air samples collected in Wando, South Korea. Phylogenetic analysis based on the internal transcribed spacer and large subunit regions revealed its unique position within the genus Acremonium. The isolated strain displayed distinct morphological characteristics, including ellipsoid or bent-ellipsoid conidia formed in clusters on the phialides. These features differentiate the new species from closely related species within the genus. This study describes the morphological and molecular characteristics of A. conglutinatum and emphasizes its phylogenetic relationships with other Acremonium spp. The identification of this novel species contributes to our understanding of the diversity and ecological role of Acremonium.

• Mycobiology
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• v.51 no.5
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• pp.281-287
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• 2023

The symbiotic association between fungus-gardening termites Macrotermes and its fungal symbiont has a moderate degree of specificity-although the symbiotic fungi (Termitomyces) form a monophyletic clade, there is not a one-to-one association between termite species and their fungus-garden associates. Here, we aim to determine the origin and phylogenetic relationships of Termitomyces in Oman. We used sequences of the internal transcribed spacer region (ITS) and the nuclear large subunit ribosomal RNA (LSU rRNA, 25S) gene and analyzed these with sequences of Termitomyces from other geographic areas. We find no evidence for more than a single colonization of Oman by Termitomyces. Unexpectedly, we find Termitomyces in Oman is most closely related to the symbiont of M. subhyalinus in West Africa rather than to those of geographically closer populations in East Africa.

• Korean journal of applied entomology
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• v.48 no.3
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• pp.369-375
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• 2009

A commercial egg parasitoid, Trichogramma sp. Nabis101, was released into agricultural cultivating areas in Korea due to its wide host spectrum against insect pests. Moreover, an application technique has been recently developed to enhance its control efficacy by mixture treatment with a microbial control agent. Despite its expansion of commercial availability, any genetic identification on this commercial strain was not determined. Also, to meet inconsistent demands from consumers, the live parasitoids need to be stored without significant loss of their survival and parasitic activity. This study determined nucleotide sequence of internal transcribed spacer (ITS) of the wasp species. The identified ITS sequences indicate that this wasp species is most similar to T brasiliensis. Optimal storage condition of this wasp required young parasitized stage at $10^{\circ}C$. Under these conditions, survival, sex ratio, longevity, and parasitic behavior were not much impaired for 5 weeks.

• Korean Journal of Plant Taxonomy
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• v.36 no.3
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• pp.189-207
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• 2006

Goodyera rosulacea, which is morphologically similar to G. repens, is described recently as a new species based on its distinct morphological characters such as rosette-formed leaves, short rhizome and habitat. To verify the taxonomic identity of G. rosulacea and its taxonomic relationship within Korean Goodyera taxa, sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA and the trnL region of cpDNA from 24 accessions including 1 outgroup accession were analyzed. Aligned sequences were analyzed using maximum parsimony and distance method, and the taxonomic identity and the taxonomic relationships among the related taxa were estimated by the existence of private marker gene and the phylogenetic tree of the aligned sequences. Molecular data indicate that G. rosulacea gas several private marker genes and shows monophyly in phylogenetic trees of both ITS and trnL sequences. the pairwise distance between G. rosulacea and the orher taxa of Korean Goodyera was 3.49-6.68% for ITS region and 5.05-9.53% for trnL region, indicating that G. rosulacea could be treated as an independent species. Therefore, our molecular data support the taxonomic of G. rosulacea as a distinct species of Korea. In phylogenetic trees, G. rosulacea formed same clade with G. repens, which has similar morphological characters with G. rosulacea, and showed the lowest pairwise distance with G. repens among Korean Goodyera taxa. These molecular data sugguested that G. rosulacea and G. repens are closely related taxa.

• The Korean Journal of Mycology
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• v.27 no.6 s.93
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• pp.406-411
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• 1999

The phylogenetic relationships of black-spored basidioid taxa (mainly coprinoid taxa) and an agaric-like gastroid taxon were studied. The sequences of internal transcribed spacer regions (ITS) partially including 17S, 25S and 5.8S from 14 species (Coprinus comatus, C. atramentarius, C. micaceus, C. cinereus, C. disseminatus, C. rhizophorus, C. radians, C. echinosporus, Psathyrella candolleana, Podaxis pistillaris, Conocybe lactea, Bolbitius demangei, Agaricus balzei, and Stropharia rugosoannulata) were compared. The reciprocal homologies of ITS sequences among these species were in the range of $38.7{\sim}77.2%$. Black-spored taxa were classified into four clusters. Cluster I comprised C. micaceus, C. radians, and C. disseminata. Cluster II is consisted of C. cinereus, C. echinosporus, C. rhizophorus, and C. atramentarius. On the other hand, C. comatus is in cluster III with Agaricus balzei and Podaxis pistillaris even though this species is belonging to the section Coprinus in morphological aspect. Psathyrelloid taxon is included in cluster II. The question of the origin of secotioid (agaric-like) fungi has been taken, though largely on theoritical suggestions as to whether gastroid taxa give rise to agarics with secotioid taxa as intermediates or whether secotioid taxa are evolutionary novelities arising from many distinct groups of agarics. In this relationship, it was shown that secotioid taxon evolved from within agaric species.