• 한국간호교육학회지
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• 제14권2호
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• pp.262-272
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• 2008

Purpose: This study examined the validity and reliability of the Korean version of the Revised Process of Change for Weight Control Scale (POC-WCS) in adults with metabolic syndrome. Method: A methodological research design with an exploratory factor analysis for validity and correlational coefficients for reliability was used. The Korean version of the Revised POC-WCS was translated into Korean and a translation equivalency was obtained. It was tested with one hundred and fifty-one obese adults with metabolic syndrome in a university hospital. The data were analyzed using Cronbach's alpha and Guttman coefficients and a principal component factor analysis with SPSS/WIN 12.0. Result: The factor analysis identified eight factors explaining 64.7% of the total variance. The Korean version of the Revised POC-WCS included stimulus control (9 items), dramatic relief (6 items), reinforcement management (6 items), helping relationships (4 items), consciousness raising (3 items), self liberation (3 items), self reevaluation (3 items), and social liberation (4 items). The internal consistency was acceptable with Cronbach's alpha (.94) and Guttman coefficient (.92). Conclusion: The Korean version of the Revised POC-WCS had adequate validity and reliability in adults with metabolic syndrome. It can be used to assess the strategies and processes for weight control in a variety of populations with obesity.

• Bulletin of the Korean Chemical Society
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• 제33권5호
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• pp.1681-1685
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• 2012

A sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to determine valproic acid in human red blood cell (RBC). It is important to measure the drug concentration of the RBC as well as that of the plasma because of drug partitioning for pharmacokinetic and pharmacodynamic study. The method was linear over the dynamic range of 1-100 ${\mu}g$/mL with a correlation coefficient $r$ = 0.9997. The linearity of this method was established from 1 to 100 ${\mu}g$/mL for valproic acid in red blood cell with accuracy and precision within 15% at all concentrations. The intra-run and inter-run assay accuracy and coefficient of variations are all within 15% for all QC samples prepared in plasma and red blood human samples. Then, valproic acid amount by protein precipitation in plasma was quantified by LC-MS/MS mass spectrometry. The distribution ratio of VPA in RBC and plasma was analyzed by clinical samples. Based on measurement of the valproic acid in human red blood cell, this method has been applied to clinical research for study of distribution ratio of valproic acid in blood.

• Parasites, Hosts and Diseases
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• 제54권1호
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• pp.1-8
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• 2016

Laboratory workers, in resource-poor countries, still consider PCR detection of Giardia lamblia more costly and more time-consuming than the classical parasitological techniques. Based on 2 published primers, an in-house one-round touchdown PCR-RFLP assay was developed. The assay was validated with an internal amplification control included in reactions. Performance of the assay was assessed with DNA samples of various purities, 91 control fecal samples with various parasite load, and 472 samples of unknown results. Two cysts per reaction were enough for PCR detection by the assay with exhibited specificity (Sp) and sensitivity (Se) of 100% and 93%, respectively. Taking a published small subunit rRNA reference PCR test results (6%; 29/472) as a nominated gold standard, G. lamblia was identified in 5.9% (28/472), 5.2%, (25/472), and 3.6% (17/472) by PCR assay, $RIDA^{(R)}$ Quick Giardia antigen detection test (R-Biopharm, Darmstadt, Germany), and iodine-stained smear microscopy, respectively. The percent agreements (kappa values) of 99.7% (0.745), 98.9% (0.900), and 97.7% (0.981) were exhibited between the assay results and that of the reference PCR, immunoassay, and microscopy, respectively. Restriction digestion of the 28 Giardia-positive samples revealed genotype A pattern in 12 and genotype B profile in 16 samples. The PCR assay with the described format and exhibited performance has a great potential to be adopted in basic clinical laboratories as a detection tool for G. lamblia especially in asymptomatic infections. This potential is increased more in particular situations where identification of the parasite genotype represents a major requirement as in epidemiological studies and infection outbreaks.

• 한국산학기술학회논문지
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• 제15권7호
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• pp.4318-4328
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• 2014

본 연구는 임상간호사의 감정노동, 직무 스트레스와 섭식태도의 정도를 파악하고 이들 간의 관련성을 파악하는 연구이다. 자료수집은 2013년 2월 21일부터 3월 31일까지이며 G시의 2개 대학병원의 간호사 434명을 대상으로 하였고 자료분석은 SPSS 17.0 프로그램을 이용하여 분석하였다. 임상간호사의 감정노동, 직무 스트레스와 섭식태도의 평균 평점은 3.53, 3.81 그리고 2.27 이었다. 감정노동은 직무스트레스((r= .570, p=<.001), 섭식태도(r= .114, p=.05)와 통계적으로 유의한 정의 상관관계가 있었다. 또한 섭식태도에 가장 큰 영향력을 미치는 요인으로는 BMI(${\beta}$=262, p<.001), 체중감량 운동을 하는 경우(${\beta}$=.203, p<.001)였으며 그다음은 특수부서 대비 내과계 병동(${\beta}$=178, p<.001), 외과계 병동(${\beta}$=.102, p=.043), 감정노동(${\beta}$=106, p=0.49)이었으며, 이 변수는 섭식태도를 16.8% 설명하였다. 따라서 섭식태도를 개선하기 위한 BMI별, 근무부서별 차별화된 관리와 프로그램이 필요할 것으로 사료된다.

• 한국전문물리치료학회지
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• 제23권2호
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• pp.84-92
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• 2016

Background: Strengthening the supraspinatus is an important aspect of a rehabilitation program for subacromial impingement and tendinopathy. Many authors recommended empty-can (EC), full-can (FC), and prone full-can (PFC) exercises to strengthen the supraspinatus. However, no ultrasonography study has yet investigated supraspinatus muscle architecture (muscle thickness; MT, pennation angle; PA, fiber bundle length; FBL) in relation to supraspinatus strengthening exercises. Objects: The purpose of this study was to compare the architecture (MT, PA, and FBL) of the supraspinatus muscle during three different types of exercises (EC, FC, and PFC) using diagnostic ultrasound. Methods: Participants performed three different exercises: (A) EC; the arm was maintained at $60^{\circ}$ abduction with full internal rotation in the sitting position, (B) FC; the arm was maintained at $60^{\circ}$ abduction with full external rotation in the sitting position, and (C) PFC; the arm was maintained at $60^{\circ}$ abduction with full external rotation in the prone position. Ultrasonography was used to measure the MT, PA and FBL of the supraspinatus. One-way repeated analysis of variance with Bonferroni's post-hoc test was used to compare between the three exercises and the initial position of each exercise. Results: Compared with each initial position, the FC exercise showed the greatest mean difference in muscle architecture properties and the PFC exercise showed the least mean difference. Conclusion: The findings suggest that the FC exercise position may have an advantage in increasing the amount of contractile tissue or producing muscle power and the PFC exercise position may be useful in a rehabilitation program because it offers the advantage of maintaining the muscle architecture properties.

• 생물정신의학
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• 제22권2호
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• pp.63-77
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• 2015

Objectives The combination extract of four kinds of herbs, Gastrodia elata, Liriope platyphylla, Dimocarpus longan, and Salvia miltiorrhiza, has shown to have memory improving effects in mice. The aim of this study was to investigate the efficacy and safety of the herbal mixture for improving working memory as well as microstructural changes in white matter integrity in individuals with subjective memory complaints. Methods Seventy-five individuals with subjective memory complaints were assigned to receive either placebo (n = 15) or herbal mixture (low-dose group, n = 30 and high-dose group, n = 30) supplementation in an 8-week, randomized, double-blind, placebo-controlled clinical trial. Changes in working memory performance and fractional anisotropy (FA) values reflecting white matter integrity from baseline to 8-week endpoint were assessed. Results The herbal mixture group showed an increase in working memory performance compared to the placebo group (p for interaction = 0.001). In addition, the herbal mixture group showed an increase in FA values in the temporo-parietal regions (corrected p < 0.05), which are crucially involved in working memory function and are among the most affected regions in patients with cognitive impairments. Conclusions Findings from this study indicate that the herbal mixture may be a promising therapeutic option for individuals with subjective memory complaints.

• 약학회지
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• 제57권5호
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• pp.330-336
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• 2013

"The Act on Medication Treatment of Sexual Impulse of Sex Offenders" known as chemical castration has been effective since July 2011 in Korea. According to the law, monitoring of male sex hormone in urine is enforced to request National Forensic Service more than once a month after injection of medicine designed to reduce sex impulse. We established a rapid and sensitive method for the monitoring of testosterone (T) and epitestosterone (E) in human urine by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Three mL of urine was pretreated by solid-phase extraction for purification and performed enzymatic hydrolysis. The pretreated samples were extracted twice with 2 ml of ethyl acetate and n-hexane (2 : 3). The separation was applied on Thermo Hypersil GOLD C18 column ($1.9{\mu}m$, $100{\times}2.1mm$). A gradient elution of methanol and water of 0.1% formic acid were used as mobile phase and the retention time was less than 10 min. LC-MS/MS system coupled with an electrospray ionization source was performed in multiple reaction monitoring mode. The transitions of the analytes executed as following: m/z $289{\rightarrow}97$, 109 for T and E, m/z $292{\rightarrow}109$ for $T-d_3$ and $E-d_3$ as internal standards. The validation results of the method were satisfactory. The limits of detection were 0.05 ng/ml and the limits of quantification were 0.1 ng/ml. This method was successfully applied to real human urine sample. The developed method will be useful for monitoring T/E ratio in urine of sex offenders.

• 한국응급구조학회지
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• 제13권1호
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• pp.61-72
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• 2009

Purpose : This study was conducted to understand the unforeseen phone calls placed through the 1339 emergency medical communication system by residents of the Gwangju/Jeonnam area during the Lunar New Year and Chuseok holidays. Methods : A descriptive research method was implemented using SPSS to analyze 9,047 reported and received consultation phone calls made during the 2008 Lunar New Year holiday (February 6-10) and the 2008 Chuseok holiday (September 13-15) for frequency and percentages. Results : Among the general characteristics of subjects during the Lunar New Year and Chuseok holidays, an examination of the distribution for gender showed more men than women and the distribution for age showed most subjects were thirties, followed by forties, and finally under ten years of age. According to the status of situational calls during the Lunar New Year holiday most were used for in the following order : providing resource information, misdialing and prank calls, and additional consultations. According to the status of situational calls during the Chuseok holiday most were used for in the following order : providing resource information, additional consultations, misdialing and prank calls. According to region most came from Gwangju Metropolitan City Bukgu, followed by Yeosu, Suncheon, and Mokpo in Jeonnam. According to callers, the majority were non-medical persons. According to time most were made between 9:01 and 12:00, while the fewest were made during 3:01 and 6:00. According to treatment department most were made in the following order : internal medicine, pharmacy, and pediatrics. Most medical resource information provided regarded areas outside the callers location. Conclusion : Emergency medical technician will play a central role in the emergency medical information system in the near future.

• 한국멀티미디어학회논문지
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• 제18권5호
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• pp.652-662
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• 2015

It is necessary to develop small, implantable bio-telemetry systems which can measure and transmit patients' bio-signals from internal body to external receiver. When measuring bio-signals, like electrical bio-signals, acoustic bio-signal measurement has also a big clinical usefulness. But, sound signal has larger frequency bandwidth than any other bio-signals. When considering these issues, a wireless telemetry system which has rapid data transmission rate proportional to wide frequency bandwidth is necessary to be developed. The bluetooth module is used to overcome the data rate limitation caused by the large frequency bandwidth. In this paper, a novel multimedia bluetooth biotelemetry system was developed which consists of transmitter module located in the body and receiver device located outside of the body. The transmitter consists of microphone, bluetooth, and wireless charging device. And the receiver consists of bluetooth and codec system. The sound inside the skin is captured by microphone and sent to receiver by bluetooth while charging. The wireless charging system constantly supplies the electric power to the system. To verify the performance of the developed system, an in vitro experiment has been performed. The results show that the proposed biotelemetry system has ability to acquire the sound signals under the skin.

• Journal of Microbiology and Biotechnology
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• 제21권1호
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• pp.5-13
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• 2011

In this study, seven Trichoderma species (33 strains) were classified using secondary metabolite profile-based chemotaxonomy. Secondary metabolites were analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS-MS) and multivariate statistical methods. T. longibrachiatum and T. virens were independently clustered based on both internal transcribed spacer (ITS) sequence and secondary metabolite analyses. T. harzianum formed three subclusters in the ITS-based phylogenetic tree and two subclusters in the metabolitebased dendrogram. In contrast, T. koningii and T. atroviride strains were mixed in one cluster in the phylogenetic tree, whereas T. koningii was grouped in a different subcluster from T. atroviride and T. hamatum in the chemotaxonomic tree. Partial least-squares discriminant analysis (PLS-DA) was applied to determine which metabolites were responsible for the clustering patterns observed for the different Trichoderma strains. The metabolites were hetelidic acid, sorbicillinol, trichodermanone C, giocladic acid, bisorbicillinol, and three unidentified compounds in the comparison of T. virens and T. longibrachiatum; harzianic acid, demethylharzianic acid, homoharzianic acid, and three unidentified compounds in T. harzianum I and II; and koninginin B, E, and D, and six unidentified compounds in T. koningii and T. atroviride. The results of this study demonstrate that secondary metabolite profiling-based chemotaxonomy has distinct advantages relative to ITS-based classification, since it identified new Trichoderma clusters that were not found using the latter approach.