• 한국표면공학회지
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• 제42권4호
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• pp.179-185
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• 2009

Diamond-like carbon(DLC) films were deposited by linear ion source(LIS)-physical vapor deposition method changing the anode voltages from 800 V to 1800 V, and characteristics of the films were investigated using residual stress tester, nano-indentation, micro raman spectroscopy, scratch tester and Field Emission Scanning Electron Microscope(FE-SEM). The results showed that the residual stress and hardness increased with increasing the ion energy up to anode voltage of 1400 V. It was also found that the content of $SP^3$ carbon increased with increasing the anode voltage $SP^3/SP^2$ ratio through investigation of $SP^3/SP^2$ ratio by the micro-raman analysis. From these results, it can be concluded that the physical properties of DLC films such as residual stress and hardness are increased with increasing the anode voltage. These results can be explained that 3-dimensional cross-links between carbon atoms and Dangling bond are enhanced and the internal compressive stress also increased with increasing the anode voltage. The optimal anode voltage is considered to be around 1400 V in these experimental conditions.

• 한국구조물진단유지관리공학회 논문집
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• 제14권2호
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• pp.121-127
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• 2010

퍼포본드 FRP 판을 영구 거푸집 및 인장 보강재로 이용한 합성보에 대한 실험을 수행하였다. FRP 판을 영구 거푸집 및 인장 보강재의 합성이용은 재래적인 방법에서 필요한 거푸집 조립 및 탈형 등의 작업이 수반되지 않으므로 빠른 시공이 가능하다. FRP 판과 콘크리트가 합성 구조체로 작용하기 위해서는 FRP 판의 표면처리가 필요하다. 이를 위해 FRP 판에 잔골재를 현장에서 많이 사용하는 에폭시를 이용하여 부착하고 FRP 판의 웨브에 구멍을 천공하였다. 합성보에 추가적인 휨 및 전단보강은 하지 않았다. 비교를 위해 두가지 종류의 비교 실험시편을 제작하였다. FRP 판의 웨브에 구멍을 천공하지 않은 비교 실험시편과 FRP 판 대신에 철근으로 보강한 비교 실험시편이다. 모든 시험체는 중앙에 집중하중을 재하하여 파괴까지 실험하였다. 본 연구 결과 퍼포본드 FRP 판은 콘크리트 구조물의 영구 거푸집 대용 및 인장 보강재로도 활용 할 수 있는 가능성을 보여주었다.

• 한국자기공명학회논문지
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• 제8권2호
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• pp.77-85
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• 2004

$^1$H-detected $^{15}$N NMR was employed to investigated the effect of mutation (Y14F) on the dynamic properties of catalytic residues in ${\Delta}^5$-3- ketosteroid isomerase (KSI) from Conamonas testosteroni. In particular, the backbone dynamics of the catalytic residues have been studied in free enzyme and its complex with a steroid ligand, 19-nortestosterone hemisuccinate, by $^{15}$N relaxation measurements. The relaxation data were analyzed using the model-free formalism to extract the model-free parameters (S$^2$, ${\tau}_e$, and R$_{ex}$). The results show that the mutation causes a significant decrease in the order parameter (S$^2$) for the catalytic residues of free Y14F KSI, presumably due to breakdown of the hydrogen bond network by mutation. In addition, the order parameters of Phe-14 and Asp-99 increased slightly upon ligand binding, indicating a slight restriction of the high-frequency (pico- to nanosecond) internal motions of the residues in the complexed Y14F KSI, while the order parameter of Tyr-55 decreased significantly upon ligand binding.

• Journal of the Korean Wood Science and Technology
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• 제21권2호
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• pp.57-65
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• 1993

The aim of this research was to manufacture sludge-particle board using paper sludge with wood particle and to investigate physical and mechanical properties of various sludge-particle boards, fabricated with ratios of sludge to particle of 10 to 90, 20 to 80, 30 to 70, 40 to 60 and 50 to 50(oven dry weight based). Sludge-particle boards were manufactured by urea-formaldehyde resin, 0.8 target specific gravity, and 10mm thickness. It was possible to manufacture sludge-particle board as the same processing in the present particleboard manufacturing system. This sludge-particle board have different properties as composition ratios of sludge and particle. And sludge-particle board made from 10 percent to 20 percent of sludge mixing ratio have similar mechanical properties compared with control particleboard. Especially, the sludge-particle board made from 10 percent to 40 percent mixing ratios of sludge have superior to control particleboard in internal bond, screw withdrawal holding strength and modulus of elasticity. In the case of dimensional stability, water absorption was increased and thickness swelling was decreased as increased with sludge mixing proportion. The sludge-particle board made of different mixing ratios of our laboratory design was able to concluded that there is possibility of partial substitution of wood particle materials.

• 대한화학회지
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• 제36권4호
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• pp.523-535
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• 1992

ECEPP/2 및 MM2 방법을 이용하여 고리형 헥사펩티드의 형태 및 알칼리 금속 양이온과의 착물형성 그리고 DPH에 의한 양이온 전달방해를 이론적으로 살펴보았다. 착물을 형성하지 않은 고리형 펩티드에 대하여 여러 가지 안정한 형태가 얻어졌으며, 대부분의 아미드 수소원자는 카르보닐 산소와 분자내 수소결합을 이루고 있어 조밀한 구조를 나타내었다. 펩티드와 Na$^+$ 이온 및 DPH간의 착물형성에너지는 각각 -60kcal/mol 및 -18kcal/mol이었다. 그러나 헥사펩티드의 국부최소에너지 구조들에는 금속 양이온을 결합할 수 있는 공동이 존재하지 않았고 공동이 존재하는 헥사펩티드의 에너지는 본 연구의 최저에너지 구조보다 10kcal/mol 정도 높은 에너지를 주었으며, 이러한 에너지 차이와 금속 양이온의 탈수 과정을 고려하면 DPH에 의한 양이온 전달방해를 설명할 수 있었다. 또한 DPH와 결합하는데 있어서의 가장 중요한 아미노산 잔기는 글리신이었으며 이를 사코신(N-메틸 글리신) 으로 치환할 때 양이온 전달이 저해되는 실험적 사실을 설명할 수 있었다.

• Journal of the Korean Wood Science and Technology
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• 제48권1호
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• pp.32-40
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• 2020

While the utilization of wood as a raw material in related industries has been increasing with the population increasing, the availability of wood from natural forests has continued to decline. An alternative to this situation is the manufacture of particleboard from non-wood lignocellulose materials through the modification of sandwich particleboard (SPb) using bamboo strands as reinforcement. In this study, strandsof belangke bamboo (Gigantochloa pruriens W) and tali bamboo (Gigantochloa apus) were utilized. The non-wood particles included sugar palm fibers, cornstalk, and sugarcane bagasse. The board was made in a three-layer composition of the face, back, and core in a ratio of 1: 2: 1. The binder used was 8% isocyanate resin. The sheet was pressed at a temperature of 160℃ for 5 min under a pressure of 3.0 N/㎟. Testing included physical and mechanical properties based on the JIS A 5908 (2003) standard, while acoustic testing was based on ISO 11654 (1997) standards. The results showed that using bamboo strands as reinforcement has an effect on the mechanical and physical properties of SPb. Almost all the types of boards met the JIS A 5908 (2003) standards, with the exception of thickness swelling (TS) and internal bond (IB) parameters. Based on the thickness swelling parameter, the C-type board exhibited the best properties. Overall, the B-type board thatused a belangke bamboo strand for the surface and sugarcane bagasse as the core underwent the best treatment. Based on the acoustical parameter, boards using a tali bamboo strand for the surface and sugar palm fiber as the core (E-type board) exhibited good sound absorption properties.

• Journal of Microbiology and Biotechnology
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• 제28권12호
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• pp.2079-2094
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• 2018

Sunflower trypsin inhibitor (SFTI) is a 14-amino-acid bicyclic peptide that contains a single internal disulfide bond. We initially constructed chimeras of SFTI with N-terminal secretion signals from the Escherichia coli OmpA and Pseudomonas aeruginosa ToxA, but only detected small amounts of protease inhibition resulting from these constructs. A substantially higher degree of protease inhibition was detected from a C-terminal SFTI fusion with E. coli YebF, which radiated more than a centimeter from an individual colony of E. coli using a culture-based inhibitor assay. Inhibitory activity was further improved in YebF-SFTI fusions by the addition of a trypsin cleavage signal immediately upstream of SFTI, and resulted in production of a 14-amino-acid, disulfide-bonded SFTI free in the culture supernatant. To assess the potential of the secreted SFTI to protect the ability of a cytotoxic protein to kill tumor cells, we utilized a tumor-selective form of the Pseudomonas ToxA (OTG-PE38K) alone and expressed as a polycistronic construct with YebF-SFTI in the tumor-targeted Salmonella VNP20009. When we assessed the ability of toxin-containing culture supernatants to kill MDA-MB-468 breast cancer cells, the untreated OTG-PE38K was able to eliminate all detectable tumor cells, while pretreatment with trypsin resulted in the complete loss of anticancer cytotoxicity. However, when OTG-PE38K was co-expressed with YebF-SFTI, cytotoxicity was completely retained in the presence of trypsin. These data demonstrate SFTI chimeras are secreted in a functional form and that co-expression of protease inhibitors with therapeutic proteins by tumor-targeted bacteria has the potential to enhance the activity of therapeutic proteins by suppressing their degradation within a proteolytic environment.

• Journal of the Korean Wood Science and Technology
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• 제32권2호
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• pp.65-72
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• 2004

요소-멜라민수지 접착제를 실험실에서 수지 접착제 고형분량에 대해 5% 멜라민을 첨가하여 파티클보드 제조용으로 합성하였다. 합성한 요소-멜라민 수지를 이용하여 전건 목재파티클의 무게에 대해 접착제 첨가량 6, 8%와 열압시간 3, 4, 5분에서 실험실 파티클보드를 접착하는 열압시간동안 배출되는 가스를 증류수에 용해·포집하여 얻어진 용액으로부터 포름알데히드 배출량을 미국 National Institute of Occupational Safety and Health (NIOSH) 3500의 방법에 의해 분석·비교하였다. 포름알데히드 배출량을 측정하여 분석한 결과는 파티클보드를 열압하는 시간동안 배출되는 가스 중에서 포름알데히드량은 요소-멜라민수지 접착제의 첨가량과 열압시간에 대해 통계적으로 유의하게 차이가 있다는 것을 보여줬다. 파티클보드의 성능평가 결과는 3분의 열압시간에서 접착된 파티클보드의 박리강도는 KS F 3104 파티클보드 8.0형의 최소요구치를 만족시키는 것을 보여줬다.

• Structural Engineering and Mechanics
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• 제85권3호
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• pp.337-351
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• 2023

In this paper, the shear behavior of soft filling in rectangular-hollow concrete specimens was simulated using the 2D particle flow code (PFC2D). The laboratory-measured properties were used to calibrate some PFC2D micro-properties for modeling the behavior of geo-materials. The dimensions of prepared and modeled samples were 100 mm×100 mm. Some disc type narrow bands were removed from the central part of the model and different lengths of bridge areas (i.e., the distance between internal tips of two joints) with lengths of 30 mm, 50 mm, and 70 mm were produced. Then, the middle of the rectangular hollow was filled with cement material. Three filling sizes with dimensions of 5 mm×5 mm, 10 mm×5 mm, and 15 mm×5 mm were provided for different modeled samples. The parallel bond model was used to calibrate and re-produce these modeled specimens. Therefore, totally, 9 different types of samples were designed for the shear tests in PFC2D. The shear load was gradually applied to the model under a constant loading condition of 3 MPa (σc/3). The loading was continued till shear failure occur in the modeled concrete specimens. It has been shown that both tensile and shear cracks may occur in the fillings. The shear cracks mainly initiated from the crack (joint) tips and coalesced with another one. The shear displacements and shear strengths were both increased as the filling dimensions increased (for the case of a bridge area with a particular fixed length).

• Journal of the Korean Wood Science and Technology
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• 제32권1호
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• pp.67-72
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• 2004

은수원사시나무 파티클과 저밀도 폴리에틸렌을 50:50, 60:40, 70:30의 3종류 혼합비율로 열압온도 145℃와 열압시간 5분에서 목질플라스틱패널을 제조하였다. 본 연구에서 목질플라스틱패널을 제조하는데 왁스와 접착제 등 어떤 첨가제도 사용하지 않았다. 제조한 목질플라스틱패널의 밀도, 박리강도, 휨탄성계수, 휨파괴계수, 두께팽창률 및 물흡수율에 대한 성질을 측정하여 패널의 성능을 평가하였다. 성능평가한 data를 SAS programing package에 의해 통계분석한 결과에 의하면 목질플라스틱패널의 박리강도, 휨파괴계수 등의 성질은 목재/폴리에틸렌 혼합비율이 50:50으로 제조된 패널이 다른 혼합비율인 60:40과 70:30으로 제조된 패널보다 5% 수준에서 높게 나타났다. 목질플라스틱패널의 치수안정화를 기준으로 볼 때, 목재/폴리에틸렌 최적의 혼합 비율이 60:40이라는 결과를 보여줬다.