• Title/Summary/Keyword: internal transcribed spacer(ITS)

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Geographic homogeneity and high gene flow of the pear psylla, $Cacopsylla$ $pyricola$ (Hemiptera: Psyllidae), detected by mitochondrial COI gene and nuclear ribosomal internal transcribed spacer 2

  • Kang, Ah-Rang;Baek, Jee-Yeon;Lee, Sang-Hyun;Cho, Young-Sik;Kim, Wol-Soo;Han, Yeon-Soo;Kim, Ik-Soo
    • Animal cells and systems
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    • v.16 no.2
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    • pp.145-153
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    • 2012
  • The pear psylla, $Cacopsylla$ $pyricola$ (Hemiptera: Psyllidae), is a serious insect pest of commercial pear crops. The species, which resides on pear trees throughout its life cycle, is rapidly spreading in some regions of the world. The population genetic structure of the species collected from several pear orchards in Korea was studied to understand the nature of dispersal and field ecology of the species. The 658-bp region of mitochondrial COI gene and the 716-bp long complete internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA were sequenced. Unlike other previously studied insect pests, the COI-based genetic diversity of the pear psylla was extremely low (maximum sequence divergence of 0.15%). This finding allowed us to conclude that the species may have been introduced in Korea relatively recently. ITS2 sequence-based analyses of phylogeny, population differentiation, gene flow, and hierarchical population structure all concordantly suggested that the pear psylla populations in Korea are neither genetically isolated nor hampered for gene flow. These genetic data are concordant with the dispersal of an overwintering winterform morph outside the non-pear habitat in the fall.

Authentication of Traded Traditional Medicine Ogapi Based on Nuclear Ribosomal DNA Internal Transcribed Spacers and Chloroplast DNA Sequences (nrDNA ITS 및 엽록체 DNA 염기서열 분석에 의한 유통 한약재 오가피 판별)

  • Kim, Jeong Hun;Byeon, Ji Hui;Park, Hyo Seop;Lee, Jeong Hoon;Lee, Sang Won;Cha, Sun Woo;Cho, Joon Hyeong
    • Korean Journal of Medicinal Crop Science
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    • v.23 no.6
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    • pp.489-499
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    • 2015
  • Background : Plants belonging to 5 species of the genus Eleutherococcus are currently distributed in the Korean peninsula. The traditional medicine 'Ogapi', derived from Eleutherococcus sessiliflorus and other related species, and 'Gasiogapi', derived from Eleutherococcus senticosus, are frequently mixed up and marketed. Therefore, accurated identification of their origins in urgently required. Methods and Results : Candidate genes from nuclear ribosomal DNA (nrDNA) and chloroplast DNA (cpDNA) of Eleutherococcus plants were analyzed. Whereas the nrDNA-internal transcribed spacer (ITS) regions were useful in elucidating the phylogenetic relationships among the plants, the cpDNA regions were not as effective. Therefore, a combined analysis with nrDNA-ITS was performed. Various combinations of nrDNA and matK were effective for discriminating among the plants. However, the matK and rpoC1 combination was ineffective for discriminating among some species. Based on these results, it was found that OG1, OG4, OG5, OG7, GS1, GS2, and GS3 were derived from E. sessiliflorus. In particular, it was confirmed that GS1, GS2, and GS3 were not derived from E. senticosus. However, more samples need to be analyzed because identification of the origins of OG2, OG3, OG6 and GS4 was not possible. Conclusion : The ITS2, ITS5a, and matK combination was the most effective in identifying the phylogenetic relationship among Eleutherococcus plants and traditional medicines based on Eleutherococcus.

The First Report of Penicillium georgiense in Malaysia

  • Yee, Teh Li;Zakaria, Latiffah
    • Mycobiology
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    • v.42 no.3
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    • pp.274-278
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    • 2014
  • Penicillium georgiense was isolated from sandy beach soil from Batu Ferringhi beach, Penang Island, Malaysia. The identification was based on morphological characteristics and phylogenetic analysis of internal transcribed spacer regions and ${\beta}$-tubulin sequences. To the best of our knowledge, this is the first report of P. georgiense in Malaysia.

First Report of Botrytis cinerea as a Postharvest Pathogen of Blueberry in Korea

  • Kwon, Jin-Hyeuk;Cheon, Mi-Geon;Choi, Ok-Hee;Kim, Jin-Woo
    • Mycobiology
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    • v.39 no.1
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    • pp.52-53
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    • 2011
  • Gray mold of blueberry caused by Botrytis sp. is reported for the first time in Korea. A detailed description of the fungus is given, along with its rDNA internal transcribed spacer sequence. The fungus was identified as Botrytis cinerea based on mycological characteristics and molecular data.

First Report of Stem Rot on Asiatic Dayflower (Commelina communis L.) Caused by Sclerotium rolfsii in Korea

  • Choi, Ok-Hee;Kwon, Jin-Hyeuk;Min, Yong-Sik;Kim, Jin-Woo
    • Mycobiology
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    • v.39 no.1
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    • pp.57-58
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    • 2011
  • Stem rot was found for the first time on the Asiatic dayflower plant (Commelina communis L.) in Korea. A detailed description of this Korean specimen is given, along with its rDNA internal transcribed spacer sequence. The fungus was identified as Sclerotium rolfsii Saccardo based on mycological characteristics and molecular data.

Soft Rot on Cucumis melo var. makuwa Caused by Rhizopus oryzae

  • Kwon, Jin-Hyeuk;Kim, Jin-Woo;Lee, Yong-Hwan;Shim, Hong-Sik
    • Mycobiology
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    • v.38 no.4
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    • pp.336-338
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    • 2010
  • Rhizopus oryzae is reported for the first time on Cucumis melo L. var. makuwa Makino. A detailed description of this Korean specimen is given, along with its rDNA internal transcribed spacer sequence. On the basis of mycological characteristics and molecular data, the fungus was identified as R. oryzae Went & Prinsen Geerligs.

Two Species of Endophytic Cladosporium in Pine Trees in Korea

  • Paul, Narayan Chandra;Yu, Seung-Hun
    • Mycobiology
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    • v.36 no.4
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    • pp.211-216
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    • 2008
  • During our studies on the diverse endophytic fungi resident on conifer needles, many species of Cladosporium previously unreported in Korea were encountered. In this paper, we report on two species of Cladosporium from the needles of pine trees (Pinus spp.). Based on analyses of internal transcribed spacer gene sequence, and cultural and micromorphological characteristics, they were identified as C. oxysporum and C. sphaerospermum. Both species have not been hitherto reported in Korea.