• Title/Summary/Keyword: internal transcribed spacer(ITS)

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A Survey of Macrofungal Diversity in Da-Lat, Southern Vietnam

  • Lee, Jin Sung;Park, Jae Young;Kim, Nam Kyu;Nguyen, Truong Binh;Kim, Minkyeong;Woo, Eun Ju;Kim, Changmu
    • The Korean Journal of Mycology
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    • v.48 no.2
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    • pp.135-149
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    • 2020
  • Da-Lat is a hilly area located in southern Vietnam. Macrofungal diversity of Da-Lat was investigated from 2018 to 2019. A total of 468 macrofungal specimens was collected and identified using the modern species concept and taxonomic and phylogenetic analyses. Among them, internal transcribed spacer(ITS) region of 401 specimens were successfully sequenced and compared with those of related species retrieved from GenBank. In total, 180 specimens were identified at the species level. The sequenced specimens were classified into 2 phyla, 13 orders, 38 families, 93 genera, and 124 species. The remaining 221 specimens (175 species) did not match the species level. This study is the first well-documented taxonomic list of macrofungi collected from southern Vietnam.

A New Record on Umbelopsis vinacea and Mucor hiemalis f. corticola Isolated from Korea

  • Das, Kallol;Ha, Ki-Hun;Suh, Sang-Jae;Lee, Seung-Yeol;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.48 no.3
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    • pp.197-207
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    • 2020
  • In the screening of fungal diversity, two strains were collected from the soil of Yeongcheon and dissected guts from the bodies of Chinese rice grasshopper (Oxya chinensis), Chinese grasshopper (Acrida cinerea), and Far eastern devil grasshopper (Oedaleus infernalis) from Daejeon in Korea. They were identified as Umbelopsis vinacea (KNU-YC-1801B) and Mucor hiemalis f. corticola (KNU-20F7, KNU-20F8, KNU-20F9). Multigene phylogenetic analyses of the internal transcribed spacer (ITS) regions, and large subunit (LSU) sequence data confirmed two unreported taxa along with their morphology. The results of molecular phylogeny firmly supported the detailed description and illustration for each taxon. As far as we know, both Umbelopsis vinacea and Mucor hiemalis f. corticola are the first reported taxa in Korea.

First Record of Endophytic Paraconiothyrium brasiliense Isolated from Chinese Maple Leaves in Korea

  • Paul, Narayan Chandra;Lee, Hyang Burm
    • The Korean Journal of Mycology
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    • v.42 no.4
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    • pp.349-352
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    • 2014
  • The fungal endophyte EML-CM25 was isolated in Korea from surface sterilized Chinese maple leaf tissues. Internal transcribed spacer sequence analysis indicated that the isolate showed 99% sequence similarity with Paraconiothyrium brasiliense, a finding that was confirmed by morphological analyses. The fungal colonies did not express aerial hyphae. Conidiomata formation was observed in the fungus cultured on potato dextrose agar at $25^{\circ}C$ for 7 days. Visible scattered black dots consisting of pycnidia were present throughout the colony. This is the first record of P. brasiliense in Korea.

New Rust Disease of Korean Willow (Salix koreensis) Caused by Melampsora yezoensis, Unrecorded Pathogen in Korea

  • Yun, Yeo Hong;Ahn, Geum Ran;Yoon, Seong Kwon;Kim, Hoo Hyun;Son, Seung Yeol;Kim, Seong Hwan
    • Mycobiology
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    • v.44 no.4
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    • pp.335-337
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    • 2016
  • During the growing season of 2015, leaf specimens with yellow rust spots were collected from Salix koreensis Andersson, known as Korean willow, in riverine areas in Cheonan, Korea. The fungus on S. koreensis was identified as the rust species, Melampsora yezoensis, based on the morphology of urediniospores observed by light and scanning electron microscopy, and the molecular properties of the internal transcribed spacer rDNA region. Pathogenicity tests confirmed that the urediniospores are the causal agent of the rust symptoms on the leaves and young stems of S. koreensis. Here, we report a new rust disease of S. koreensis caused by the rust fungus, M. yezoensis, a previously unrecorded rust pathogen in Korea.

Prionchulus oleksandri (Nematoda: Mononchida) from Korea

  • Kim, Jiyeon;Kim, Taeho;Ryu, Shi Hyun;Park, Joong-Ki
    • Animal Systematics, Evolution and Diversity
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    • v.34 no.4
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    • pp.194-198
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    • 2018
  • The genus Prionchulus Cobb, 1916 represents a group of predaceous nematodes belonging to the family Mononchidae Chitwood, 1937, and is found worldwide. However, only five species have been reported thus far from Korea. Prionchulus oleksandri Winiszewska and Susulovsky, 2003 is reported for the first time from Korea, from sediments collected from the Nakdong River. This species is distinguished from other Prionchulus species by its truncated lip region with small cephalic papillae and refringens vaginae. In this study, morphological characters(detailed morphometrics) of P. oleksandri are described and illustrated using optical microscopy. DNA barcode sequence information (the D2-D3 region of 28S rDNA, 18S rDNA, and internal transcribed spacer rDNA) is also provided for the molecular identification of the species.

First Report of Albifimbria verrucaria and Deconica coprophila (Syn: Psylocybe coprophila) from Field Soil in Korea

  • Gurung, Sun Kumar;Adhikari, Mahesh;Kim, Sang Woo;Lee, Hyun Goo;Jun, Ju Han;Gwon, Byeong Heon;Lee, Hyang Burm;Lee, Youn Su
    • The Korean Journal of Mycology
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    • v.47 no.3
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    • pp.209-218
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    • 2019
  • During a survey of fungal diversity in Korea, two fungal strains, KNU17-1 and KNU17-199, were isolated from paddy field soil in Yangpyeong and Sancheong, respectively, in Korea. These fungal isolates were analyzed based on their morphological characteristics and the molecular phylogenetic analysis of the internal transcribed spacer (ITS) rDNA sequences. On the basis of their morphology and phylogeny, KNU17-1 and KNU17-199 isolates were identified as Albifimbria verrucaria and Deconica coprophila, respectively. To the best of our knowledge, A. verrucaria and D. coprophila have not yet been reported in Korea. Thus, this is the first report of these species in Korea.

Seven New Records of Penicillium Species Belonging to Section Lanata-Divaricata in Korea

  • Pangging, Monmi;Nguyen, Thuong T.T.;Lee, Hyang Burm
    • Mycobiology
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    • v.49 no.4
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    • pp.363-375
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    • 2021
  • Penicillium species are known to be ubiquitous environmental saprophytes. In the survey of diversity of genus Penicillium, seven new records of Penicillium species belonging to section Lanata-Divaricata were isolated from freshwater and soil samples collected from different locations in Korea. Based on morphological characteristics and multilocus phylogenetic analysis of the rDNA internal transcribed spacer region (ITS), β-tubulin (BenA), and calmodulin (CaM) genes, the isolated strains were identified as P. annulatum, P. camponotum, P. echinulonalgiovense, P. globosum, P. limosum, P. onobense, and P. yunnanense, respectively. This study presents detailed phylogenetic analyses and morphological descriptions of these species that contribute to section Lanata-Divaricata in Korea.

Acaulospora jejuensis, a New Species in Glomeromycota from Korea

  • Park, Hyeok;Ka, Kang-Hyeon;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.49 no.4
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    • pp.425-431
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    • 2021
  • New species of arbuscular mycorrhizal fungi (Glomeromycota), Acaulospora jejuensis, was isolated from rhizosphere soils of Miscanthus sinensis in the grassland in Jeju Island of Korea. The species was identified using the morphological characteristics of the spores and the molecular phylogenetic analysis using partial DNA sequences from small subunit rDNA (SSU), internal transcribed spacer (ITS), and large subunit rDNA (LSU). Phylogenetic analysis placed the fungal species in a distinct clade within genus Acaulospora. Also, the species exhibited the morphological characteristics distinct from the other members of the genus. Therefore, Acaulospora jejuensis was described as a novel species from Korea.

Fulvifomes nonggangensis and F. tubogeneratus (Hymenochaetales, Basidiomycota): Two New Species from Southern China Based on Morphological and Molecular Evidences

  • Zheng, Hai-Fu;Huang, Fu-Chang;Liu, Bin;Shao, Yuan-Yuan;Qin, Pei-Sheng
    • Mycobiology
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    • v.49 no.3
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    • pp.213-222
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    • 2021
  • Two new species of Fulvifomes are described from specimens collected in rainforests of Nonggang Nature Reserve of southern China, based on morphological characteristics and molecular phylogenetic analysis of the internal transcribed spacer (ITS) and nuclear large subunit ribosomal DNA (nLSU) sequences. Fulvifomes nonggangensis sp. nov. is characterized by perennial, sessile and solitary basidiocarps, applanate pileus, small cystidioles of 9.9-15.4 × 2.9-3.5 ㎛, large pores of 5-6 per mm, a dimitic hyphal system, and broadly ellipsoid basidiospores of 4.3-5.3 × 3.3-4.2 ㎛. F. tubogeneratus sp. nov. is characterized by perennial, sessile, and imbricate basidiocarps, a duplex context, small pores of 7-8 per mm, a dimitic hyphal system, and ovoid to subglobose basidiospores of 5.72 × 5.00 ㎛.

Diversity of Endophytic Fungi Isolated from Pinus densiflora and Juniperus rigida Distributed in Mt. Baekryeonsan and Mt. Johangsan, Korea

  • Eo, Ju-Kyeong;Park, Hyeok;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.46 no.4
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    • pp.437-446
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    • 2018
  • We investigated the biodiversity of endophytic fungi in Pinus densiflora and Junipreus rigida from Mt. Baekryeonsan and Mt. Johangsan in Korea. In total, 59 isolates were discovered from 26 host plants and identified using sequences from the rDNA internal transcribed spacer (ITS) region. Twenty taxa of endophytic fungi were identified in this study; 62% of isolates belonged to Leotiomycetes, 34% belonged to Sordariomyetes, and 4% belonged to Agaricomycetes. Results confirmed three previously unreported endophytic fungi in Korea: Phanerochaete concrescens, Creosphaeria sassafras, and Scopuloides rimosa. Community analysis showed that the fungal diversity patterns of the samples from Mt Baekryeonsan and Mt. Johangsan are similar to those from previous studies and varied depending upon host and locality.