• Title/Summary/Keyword: internal transcribed spacer(ITS)

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Four Species of Montagnulaceae Unrecorded in Korea and Isolated from Plant Litter in Freshwater

  • Goh, Jaeduk;Mun, Hye Yeon;Oh, Yoosun;Chung, Namil
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.263-270
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    • 2016
  • Freshwater is a diverse and complex environment for fungi and provides several types of habitat including plant litter, sediment, and carcasses. We collected plant litter from the main stream and branch streams of Nakdong River, Sohancheon in Samcheok, and Geumoreum in Jeju. From several samples of plant litter, we isolated 8 fungal strains belonging to 4 Montagnulaceae species unrecorded in Korea: Paraconiothyrium archidendri, Paraphaeosphaeria sporulosa, Paraphaeosphaeria michotii, and Paraphaeosphaeria viridescens. These fungi were identified by phylogenetic analysis using the internal transcribed spacer (ITS) region and examination of morphological characteristics. Montagnulaceae is known as one of the families in Pleosporales and includes coniothyrium-like fungi. In this study, we described phylogenetic analysis and mycological characteristics of these species, and this is the first report of these taxa in Korea.

First Report of Neopestalotiopsis australis Isolated from Soil in Korea

  • Park, Sangkyu;Lee, Seung-Yeol;Lee, Jae-Jin;Back, Chang-Gi;Ten, Leonid;Lee, Hyang Burm;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.360-364
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    • 2016
  • Fungal strain KNU16-005 was isolated from field soil in Jeollabuk-do, Korea. Based on its morphological characteristics and phylogenic analysis using internal transcribed spacer regions of rDNA, ${\beta}$-tubulin and translation elongation factor 1-alpha gene sequences of KNU16-005 were identified as Neopestalotiopsis australis. This species has not been previously reported in Korea.

Molecular Characterization and Morphology of Two Endophytic Peyronellaea Species from Pinus koraiensis in Korea

  • Deng, Jian Xin;Paul, Narayan Chandra;Li, Mei Jia;Seo, Eun-Young;Sung, Gi-Ho;Yu, Seung-Hun
    • Mycobiology
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    • v.39 no.4
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    • pp.266-271
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    • 2011
  • Species of Phoma and its allies were isolated during a survey on the diversity of endophytic fungi associated with pine trees in Korea. Based on the phylogenetic analyses of internal transcribed spacer and ${\beta}$-tubulin gene sequences, two Phoma-like species from the needles of Pinus koraiensis were identified as Peyronellaea calorpreferens and P. glomerata. They were also morphologically identified based on the previous descriptions. Here, we report P. calorpreferens and P. glomerata being present in Korea as endophytic fungi in Pinus koraiensis.

First Record of Mattirolomyces terfezioides and Tricholoma bakamatsutake in Korea (한국에서 Mattirolomyces terfezioides와 Tricholoma bakamatsutake의 보고)

  • Ka, Kang-Hyeon;Jeon, Sung-Min;Ryoo, Rhim;Kang, Jung-A;Hong, Ki-Sung
    • The Korean Journal of Mycology
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    • v.43 no.2
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    • pp.125-128
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    • 2015
  • Mattirolomyces terfezioides and Tricholoma bakamatsutake, commercially important mycorrhizal mushrooms, were found for the first time in the forests of Robinia pseudoacacia and Quercus mongolica of the Korean peninsula, respectively. Morphological and molecular characteristics were discussed in the paper. We have also given the Korean name to the fungi here.

Entomopathogenicity of Simplicillium lanosoniveum Isolated in Korea

  • Lim, Sung Yeol;Lee, Sehee;Kong, Hyun Gi;Lee, Jungkwan
    • Mycobiology
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    • v.42 no.4
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    • pp.317-321
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    • 2014
  • Fruiting bodies similar to those of the ascomycete fungi Podostroma cornu-damae and Cordyceps militaris were collected from Mt. Seunghak in Busan, Korea on August 21, 2012. The fruiting bodies were cylindrical, with tapered ends and golden red in color. The fruiting bodies contained abundant conidiophores bearing single-celled conidia, but no perithecia or asci. Pure culture of the fungal isolates was obtained through single-spore isolation. Analyses of morphological characteristics, including conidia shape, and phylogenetic traits, using internal transcribed spacer sequences, showed that these isolates belonged to the species Simplicillium lanosoniveum. Although this fungal species is known to be mycoparasitic, the isolates obtained in this study were unable to infect fungi. However, silkworms (Bombyx mori) inoculated with the fungal isolates died during the larval or pupal stages, as has been shown for the strongly entomopathogenic fungus Beauveria bassiana. This study is the first report of the entomopathogenicity of S. lanosoniveum and indicates its potential for use in biological control of insects.

First Report of Simplicillium cylindrosporum Isolated from Soil in Korea

  • Park, Sangkyu;Lee, Seung-Yeol;Lee, Jae-Jin;Lee, Hyang Burm;Ten, Leonid;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.45 no.2
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    • pp.139-144
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    • 2017
  • The fungal strain KNU16-006 was isolated from field soil in Daegu, Korea. The isolate was identified as Simplicillium cylindrosporum on the basis of its morphological characterization and phylogenetic analysis using internal transcribed spacer regions of rDNA. This species has not been previously reported in Korea.

Test of the hybrid origin of Broussonetia × kazinoki (Moraceae) in Korea using molecular markers

  • WON, Hyosig
    • Korean Journal of Plant Taxonomy
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    • v.49 no.4
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    • pp.282-293
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    • 2019
  • Broussonetia × kazinoki Siebold has long been utilized as a major component in the manufacturing of Korean traditional paper, hanji, and has been suggested as a hybrid species of B. papyrifera and B. monoica. By applying three molecular markers, chloroplast (cp) ndhF-rpl32 IGS, a nuclear ribosomal internal transcribed spacer, and the TOPO6 gene, the hybrid origin of B. × kazinoki is tested. As a result, B. × kazinoki in Korea is demonstrated to be a hybrid of B. monoica × B. papyrifera, most likely formed naturally in Korea. The cp haplotypes detected provided information about the origins and genetic diversity of the maternal lineage B. monoica and paternal lineage B. papyrifera. The two nuclear markers were supplemented to each other, leading to the discovery of introgression in Broussonetia.

Diversity of Ectomycorrhizal fungi of Abies koreana at Mt. Halla (한라산 구상나무(Abies koreana)의 외생균근의 다양성)

  • Sim, Mi-Young;Eo, Ju-Kyeong;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.37 no.2
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    • pp.134-138
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    • 2009
  • This study was conducted to investigate colonization of ectomycorrhizal fungi(ECM) in roots of Abies koreana which is an endemic and endangered species in Korea. Roots of A. koreana were collected at Mt. Halla. ECM root tips were classified using morphotyping and identified using sequences of internal transcribed spacer (ITS) region of the fungal rDNA. Total 8 species of ECM fungi were identified from roots of 11 seedlings of A. koreana : Cenococum geophilum, Russula brevipes, 2 species of Russula, 2 species of Thelephora, Cortinarius camphorates and 2 species of Helotiales. These species were known to be typical ectomycorrhizal fungi found in coniferous mature forests.

Diversity and Seasonal Variation of Endophytic Fungi Isolated from Three Conifers in Mt. Taehwa, Korea

  • Kim, Chang-Kyun;Eo, Ju-Kyeong;Eom, Ahn-Heum
    • Mycobiology
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    • v.41 no.2
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    • pp.82-85
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    • 2013
  • The needled leaves of three conifer species were collected in Mt. Taehwa during different seasons of the year. Total 59 isolates and 19 species of endophytic fungi were isolated from the leaves and identified using morphological and molecular characteristics. As a result, Shannon index was different in its host plant; Larix kaempferi had a highest value of species diversity. According to the sampling season, 9 species of 19 species were isolated during fall season. The results suggest that the existing of host plant and sampling season are major factors of distribution of endophytic fungi.

A New Record of Pseudeurotium bakeri from Crop Field Soil in Korea

  • Adhikari, Mahesh;Kim, Sangwoo;Yadav, Dil Raj;Um, Yong Hyun;Kim, Hyun Seung;Lee, Hyang Burm;Lee, Youn Su
    • The Korean Journal of Mycology
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    • v.44 no.3
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    • pp.145-149
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    • 2016
  • Pseudeurotium bakeri KNU14-21 isolated from crop field soil is a previously unreported fungal species in Korea. This fungal species was identified through morphological and molecular characteristics. Based on the shape and size of conidia and conidiophores as well as the internal transcribed spacer region of rDNA, the isolate was confirmed to be Pseudeurotium bakeri. This species has not been previously reported from Korea, and this is the first report of its incidence in Korea.