• Title/Summary/Keyword: internal transcribed spacer(ITS)

Search Result 650, Processing Time 0.023 seconds

First Report of Rhizopus oryzae as a Postharvest Pathogen of Apple in Korea

  • Kwon, Jin-Hyeuk;Kim, Jin-Woo;Kim, Won-Il
    • Mycobiology
    • /
    • v.39 no.2
    • /
    • pp.140-142
    • /
    • 2011
  • Soft rot in apple caused by Rhizopus oryzae was found for the first time in Korea. A detailed description of the specimen is given along with its internal transcribed spacer rDNA sequence. The fungus was identified as Rhizopus oryzae based on the mycological characteristics, molecular data, and pathogenicity testing.

A New Record of Penicillium pimiteouiense from Beach Soil in Malaysia

  • Teh, Li Yee;Latiffah, Zakaria
    • Mycobiology
    • /
    • v.41 no.4
    • /
    • pp.256-259
    • /
    • 2013
  • Three isolates of Penicillium pimiteouiense were recovered from sandy beach soil samples in Penang Island, Peninsular Malaysia. All the isolates were identified based on morphological characteristics and phylogenetic analysis of internal transcribed spacer regions and ${\beta}$-tubulin gene. This is a first record of P. pimiteouiense in Malaysia.

Molecular Identification of Mycorrhizae of Cymbidium kanran (Orchidaceae) on Jeju Island, Korea

  • Hong, Ji Won;Suh, Hyoungmin;Kim, Oh Hong;Lee, Nam Sook
    • Mycobiology
    • /
    • v.43 no.4
    • /
    • pp.475-480
    • /
    • 2015
  • A fungal internal transcribed spacer region was used to identify the mycorrhizae of Cymbidium kanran. The family Russulaceae was found to be the most frequently occurring group in both root and soil samples. In phylogenetic analyses, the majority of the Russulaceae clones were clustered with Russula brevipes and R. cyanoxantha. Therefore, C. kanran may form symbiotic relationships with the genus Russula.

First Report of Leptosphaerulina australis Isolated from Soil in Korea

  • Li, Weilan;Back, Chang-Gi;Lee, Seung-Yeol;Ten, Leonid N.;Jung, Hee-Young
    • The Korean Journal of Mycology
    • /
    • v.46 no.4
    • /
    • pp.369-374
    • /
    • 2018
  • The fungal strain KNU16-004 was isolated from a field soil sample collected in Seoul. The isolate was identified as Leptosphaerulina australis based on morphological characterization and phylogenetic analysis using the internal transcribed spacer (ITS), large subunit (LSU) rDNA regions, and ${\beta}-tubulin$ (Tub2). This is the first report of Leptosphaerulina australis in Korea.

One Unrecorded Endophytic Fungi from Sub-alpine Conifer, Rhizosphaera pini

  • Eo, Ju-Kyeong;Park, Eunsu
    • The Korean Journal of Mycology
    • /
    • v.47 no.2
    • /
    • pp.121-124
    • /
    • 2019
  • An endophytic fungus, Rhizosphaera pini strain NIE7426, was isolated from the sub-alpine coniferous tree Abies nephrolepis in Mt. Nochu of Gangwon Province. It was characterized by macroscopic and microscopic features, as well as the internal transcribed spacer (ITS) 1, 2 and 5.8S sequences. All morphological and molecular features support the first recognition of this taxon in Korea. In addition, this study adds A. nephrolepis as a host plant R. pini.

First Reports of Unrecorded Mortierellomycetes and Umbelopsidomycetes Fungi from Freshwater Ecosystems in Korea

  • Jaeduk Goh;Yoosun Oh;Hye Yeon Mun
    • The Korean Journal of Mycology
    • /
    • v.51 no.3
    • /
    • pp.155-165
    • /
    • 2023
  • In this study, we isolated several fungal strains from filtered water and sediment collected from rivers and streams. The strains were identified by molecular phylogenetic analyses of rDNA sequences (internal transcribed spacer [ITS], large subunit of ribosomal DNA [LSU]). The morphological characteristics of the fungi were investigated using microscopy, and the culture characteristics of fungi grown on several media were examined. We identified four species previously unknown in South Korea, namely, Dissophora globulifera, Linnemannia exigua, Mortierella rishikesha and Umbelopsis autotrophica.

Isolation and Identification of Wild Yeasts from Soils of Fields in Daejeon Metropolitan City and Chungcheongnam-do, Korea (대전광역시와 충청남도 밭 토양으로부터 야생효모의 분리 및 동정)

  • Han, Sang-Min;Han, Jae-Won;Bae, Sang-Min;Park, Won-Jong;Lee, Jong-Soo
    • The Korean Journal of Mycology
    • /
    • v.44 no.1
    • /
    • pp.1-7
    • /
    • 2016
  • This study focused on isolation and identification of wild yeasts from soils in fields near mountains and elucidation of its yeast distribution. Several kinds of yeasts were isolated from various soils of Daejeon metropolitan city and Chungcheongnam-do in Korea and identified by BLAST search of nucleotide sequences of internal transcribed spacer (ITS) region including 5.8S rRNA and D1/D2 region of 26S rDNA. Ninety-seven strains of 20 species from 61 soil samples were isolated, of which Cryptococcus podzolicus (11 strains), Debaryomyces hansenii (6 strains), and Trichosporon asahii (6 strains) were dominant species.

Phylogeny of Korean Isolates of Phytophthora Species Based on Sequence Analysis of Internal Transcribed Spacer of Ribosomal DNA

  • Hong, Seung-Beom;Jee, Hyeong-Jin;Kim, Sang-Hee;Go, Seung-Joo
    • The Plant Pathology Journal
    • /
    • v.16 no.1
    • /
    • pp.29-35
    • /
    • 2000
  • The internal transcribed spacer regions (ITS I, 5.8S and ITS II) of the ribosomal DNAs were amplified from Korean isolates of Phytophthora spp. and sequenced to characterize them. Sequences from 33 isolates previously identified as P. boehmeriae, P. cactprum, P. cambivora, P. capsici, P. cinnamomi, P. erythroseptica, P. infestans, P. megasperma, P. melonis, P. nicotianae, P. palmivora and P. sojae were compared with published sequences, and a phylogenetic tree was produced. All isolates belonging to 10 species, P. cactorum, P. cambivora, P. capsici, P. cinnamomi P. citricola, P. infestans, P. nicotianae, P. palmivora and P. sojae were clearly clustered into published isolates of each species above 97% bootstrap value. Cucurbits isolates of Phytophthora previously identified as either P. melonis or P. drechsleri showed distinct evolutionary lineages from the P. megasperma was closely related to isolates of P. cryptogea-P. drechsleri showed distinct evolutionary lineages from the P. cryptogea-P. drechsleri complex group, indicating that P. melonis is a valid species. A Korean isolate of P. megasperma was closely related to isolates of P. erythroseptica showed distant genetic relationship with published isolates of P. erythroseptica (CBS 956.87). It is probable that the two Korean isolates could be genetically different from foreign isolates or misidentified. A grouping of species according to ITS sequence divergence matched, to some degree, the broad classification based on type of papilla. However, a separation of semi-papillate species and papillate species was not wvident in this study.

  • PDF

Identification of Unrecorded Endophytic Fungi Isolated from Leaves of Woody Plants in Jejudo, Korea (제주도에 서식하는 목본 식물의 잎에서 분리한 미기록 내생균)

  • Lee, Bong-Hyung;Kim, Dong-Yeo;Park, Hyeok;Eo, Ju-Kyeong;Lee, Hyang Burm;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.44 no.4
    • /
    • pp.252-258
    • /
    • 2016
  • In this study, endophytic fungi were isolated from the leaves of five species of woody plants in Jeju, Korea, namely Chamaecyparis obtusa, Cryptomeria japonica, Torreya nucifera, Ilex crenata, and Camellia japonica. The isolated fungal endophytes were identified based on their morphological and molecular characteristics including a sequence analysis of the internal transcribed spacer and 26S regions of rDNA and ${\beta}$-tubulin genes. Ten species of fungal endophytes have not been previously reported in Korea, namely Mycosphaerella aleuritidis, Neofusicoccum eucalyptorum, Neofusicoccum parvum, Phyllosticta citrichinensis, Phyllosticta cryptomeriae, Phomopsis cotoneastri, Sphaerulina rhododendricola, Guignardia mangiferae, Lophodermium jiangnanense, and Lophodermium minus.

Notes on Five Unrecorded Endophytic Fungi Isolated from Coniferous Leaves and Orchid Roots in Korea (한국에 서식하는 침엽수의 잎과 난초과 식물의 뿌리에서 분리한 5종의 국내 미기록 내생균)

  • Park, Hyeok;Lee, Bong-Hyung;Bae, Yu-Ra;Kim, Dong-Yeo;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.44 no.4
    • /
    • pp.365-370
    • /
    • 2016
  • We collected leaves of Pinus koraiensis and Thuja koraiensis and roots of Bletilla striata from various sites in Korea. The leaf and root samples were surface-sterilized and endophytic fungi were isolated. Fungal isolates were identified based on their morphological characteristics and a phylogenetic analysis of internal transcribed spacer regions, large subunit regions, and the ${\beta}$-tubulin gene. Consequently, we identified five species of endophytic fungi, namely Colletotrichum simmondsii, Fusarium sterilihyphosum, Diatrypella pulvinata, Ochroconis globalis, and Sphaeria chrysosperma. These species have not been previously reported in Korea and we report them here with descriptions and illustrations.